Efficient extraction of fructans from sotol plant (Dasylirion leiophyllum) enhanced by a combination of enzymatic and sonothermal treatments.

Autores
Pardo Rueda, Abril J.; Quintero Ramos, Armando; Genovese, Diego Bautista; Camacho Dávila, Alejandro; Zepeda Rodríguez, Armando; Contreras Esquivel, Juan C.; Bizarro, A. Patricia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effects of different extraction methods of water-soluble carbohydrates (WSC) from the sotol plant (Dasylirion leiophyllum) were investigated. Sotol fragments were extracted at 40 and 70 °C, under thermal treatment (T), pre-enzymatic thermal treatment (PET), sonothermal treatment (ST), and pre-enzymatic sonothermal treatment (PEST) conditions: fructose, glucose, sucrose, and fructans were analyzed by HPLC and the total water soluble carbohydrates was determined. At 70 °C, the highest WSC values (482 mg/gd.m.) were obtained, with a fructan proportion of 69%. Pre-enzymatic treatment at 70 °C resulted in a high WSC content with the highest fructans proportion (87%) and lowest contents of RS and sucrose. The effect of the interaction between ultrasound and enzymatic treatments was limited by the high-temperature effect (70 °C), thereby minimizing the extraction. Microscopy analyses showed cell-wall modifications with the ST and PET treatments, which caused an increase in the total soluble sugars. The combination of enzymatic and sonothermal treatments at 70 °C resulted in the extraction of fructans in a higher yield and with less degradation. This circumvents the need for traditional high-energy processes, which could be beneficial for the extraction of WSC such as fructans from sotol or other economically important plants.
Fil: Pardo Rueda, Abril J.. Universidad Autónoma de Chihuahua. Facultad de Ciencias Químicas; México
Fil: Quintero Ramos, Armando. Universidad Autónoma de Chihuahua. Facultad de Ciencias Químicas; México
Fil: Genovese, Diego Bautista. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Camacho Dávila, Alejandro. Universidad Autónoma de Chihuahua. Facultad de Ciencias Químicas; México
Fil: Zepeda Rodríguez, Armando. Universidad Nacional Autónoma de México; México
Fil: Contreras Esquivel, Juan C.. Universidad Autónoma de Coahuila. Facultad de Ciencias Químicas; México
Fil: Bizarro, A. Patricia. Universidad Nacional Autónoma de México; México
Materia
Dasylirion Leiophyllum; Carbohydrates; E; Ultrasound; E; F
Carbohydrates
Extraction
Ultrasound
Enzyme
Fructans
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13470
Acceder
id CONICETDig_c31b601fab84116c149ebcc02555aaff
oai_identifier_str oai:ri.conicet.gov.ar:11336/13470
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Efficient extraction of fructans from sotol plant (Dasylirion leiophyllum) enhanced by a combination of enzymatic and sonothermal treatments.Pardo Rueda, Abril J.Quintero Ramos, ArmandoGenovese, Diego BautistaCamacho Dávila, AlejandroZepeda Rodríguez, ArmandoContreras Esquivel, Juan C.Bizarro, A. PatriciaDasylirion Leiophyllum; Carbohydrates; E; Ultrasound; E; FCarbohydratesExtractionUltrasoundEnzymeFructanshttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2The effects of different extraction methods of water-soluble carbohydrates (WSC) from the sotol plant (Dasylirion leiophyllum) were investigated. Sotol fragments were extracted at 40 and 70 °C, under thermal treatment (T), pre-enzymatic thermal treatment (PET), sonothermal treatment (ST), and pre-enzymatic sonothermal treatment (PEST) conditions: fructose, glucose, sucrose, and fructans were analyzed by HPLC and the total water soluble carbohydrates was determined. At 70 °C, the highest WSC values (482 mg/gd.m.) were obtained, with a fructan proportion of 69%. Pre-enzymatic treatment at 70 °C resulted in a high WSC content with the highest fructans proportion (87%) and lowest contents of RS and sucrose. The effect of the interaction between ultrasound and enzymatic treatments was limited by the high-temperature effect (70 °C), thereby minimizing the extraction. Microscopy analyses showed cell-wall modifications with the ST and PET treatments, which caused an increase in the total soluble sugars. The combination of enzymatic and sonothermal treatments at 70 °C resulted in the extraction of fructans in a higher yield and with less degradation. This circumvents the need for traditional high-energy processes, which could be beneficial for the extraction of WSC such as fructans from sotol or other economically important plants.Fil: Pardo Rueda, Abril J.. Universidad Autónoma de Chihuahua. Facultad de Ciencias Químicas; MéxicoFil: Quintero Ramos, Armando. Universidad Autónoma de Chihuahua. Facultad de Ciencias Químicas; MéxicoFil: Genovese, Diego Bautista. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. Universidad Nacional del Sur; ArgentinaFil: Camacho Dávila, Alejandro. Universidad Autónoma de Chihuahua. Facultad de Ciencias Químicas; MéxicoFil: Zepeda Rodríguez, Armando. Universidad Nacional Autónoma de México; MéxicoFil: Contreras Esquivel, Juan C.. Universidad Autónoma de Coahuila. Facultad de Ciencias Químicas; MéxicoFil: Bizarro, A. Patricia. Universidad Nacional Autónoma de México; MéxicoElsevier Science2015-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13470Pardo Rueda, Abril J.; Quintero Ramos, Armando; Genovese, Diego Bautista; Camacho Dávila, Alejandro; Zepeda Rodríguez, Armando; et al.; Efficient extraction of fructans from sotol plant (Dasylirion leiophyllum) enhanced by a combination of enzymatic and sonothermal treatments.; Elsevier Science; Food And Bioproducts Processing; 94; 4-2015; 398-4040960-3085enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S096030851400056Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.fbp.2014.05.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:59:03Zoai:ri.conicet.gov.ar:11336/13470instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:59:03.559CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Efficient extraction of fructans from sotol plant (Dasylirion leiophyllum) enhanced by a combination of enzymatic and sonothermal treatments.
title Efficient extraction of fructans from sotol plant (Dasylirion leiophyllum) enhanced by a combination of enzymatic and sonothermal treatments.
spellingShingle Efficient extraction of fructans from sotol plant (Dasylirion leiophyllum) enhanced by a combination of enzymatic and sonothermal treatments.
Pardo Rueda, Abril J.
Dasylirion Leiophyllum; Carbohydrates; E; Ultrasound; E; F
Carbohydrates
Extraction
Ultrasound
Enzyme
Fructans
title_short Efficient extraction of fructans from sotol plant (Dasylirion leiophyllum) enhanced by a combination of enzymatic and sonothermal treatments.
title_full Efficient extraction of fructans from sotol plant (Dasylirion leiophyllum) enhanced by a combination of enzymatic and sonothermal treatments.
title_fullStr Efficient extraction of fructans from sotol plant (Dasylirion leiophyllum) enhanced by a combination of enzymatic and sonothermal treatments.
title_full_unstemmed Efficient extraction of fructans from sotol plant (Dasylirion leiophyllum) enhanced by a combination of enzymatic and sonothermal treatments.
title_sort Efficient extraction of fructans from sotol plant (Dasylirion leiophyllum) enhanced by a combination of enzymatic and sonothermal treatments.
dc.creator.none.fl_str_mv Pardo Rueda, Abril J.
Quintero Ramos, Armando
Genovese, Diego Bautista
Camacho Dávila, Alejandro
Zepeda Rodríguez, Armando
Contreras Esquivel, Juan C.
Bizarro, A. Patricia
author Pardo Rueda, Abril J.
author_facet Pardo Rueda, Abril J.
Quintero Ramos, Armando
Genovese, Diego Bautista
Camacho Dávila, Alejandro
Zepeda Rodríguez, Armando
Contreras Esquivel, Juan C.
Bizarro, A. Patricia
author_role author
author2 Quintero Ramos, Armando
Genovese, Diego Bautista
Camacho Dávila, Alejandro
Zepeda Rodríguez, Armando
Contreras Esquivel, Juan C.
Bizarro, A. Patricia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Dasylirion Leiophyllum; Carbohydrates; E; Ultrasound; E; F
Carbohydrates
Extraction
Ultrasound
Enzyme
Fructans
topic Dasylirion Leiophyllum; Carbohydrates; E; Ultrasound; E; F
Carbohydrates
Extraction
Ultrasound
Enzyme
Fructans
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The effects of different extraction methods of water-soluble carbohydrates (WSC) from the sotol plant (Dasylirion leiophyllum) were investigated. Sotol fragments were extracted at 40 and 70 °C, under thermal treatment (T), pre-enzymatic thermal treatment (PET), sonothermal treatment (ST), and pre-enzymatic sonothermal treatment (PEST) conditions: fructose, glucose, sucrose, and fructans were analyzed by HPLC and the total water soluble carbohydrates was determined. At 70 °C, the highest WSC values (482 mg/gd.m.) were obtained, with a fructan proportion of 69%. Pre-enzymatic treatment at 70 °C resulted in a high WSC content with the highest fructans proportion (87%) and lowest contents of RS and sucrose. The effect of the interaction between ultrasound and enzymatic treatments was limited by the high-temperature effect (70 °C), thereby minimizing the extraction. Microscopy analyses showed cell-wall modifications with the ST and PET treatments, which caused an increase in the total soluble sugars. The combination of enzymatic and sonothermal treatments at 70 °C resulted in the extraction of fructans in a higher yield and with less degradation. This circumvents the need for traditional high-energy processes, which could be beneficial for the extraction of WSC such as fructans from sotol or other economically important plants.
Fil: Pardo Rueda, Abril J.. Universidad Autónoma de Chihuahua. Facultad de Ciencias Químicas; México
Fil: Quintero Ramos, Armando. Universidad Autónoma de Chihuahua. Facultad de Ciencias Químicas; México
Fil: Genovese, Diego Bautista. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Planta Piloto de Ingeniería Química (i); Argentina. Universidad Nacional del Sur; Argentina
Fil: Camacho Dávila, Alejandro. Universidad Autónoma de Chihuahua. Facultad de Ciencias Químicas; México
Fil: Zepeda Rodríguez, Armando. Universidad Nacional Autónoma de México; México
Fil: Contreras Esquivel, Juan C.. Universidad Autónoma de Coahuila. Facultad de Ciencias Químicas; México
Fil: Bizarro, A. Patricia. Universidad Nacional Autónoma de México; México
description The effects of different extraction methods of water-soluble carbohydrates (WSC) from the sotol plant (Dasylirion leiophyllum) were investigated. Sotol fragments were extracted at 40 and 70 °C, under thermal treatment (T), pre-enzymatic thermal treatment (PET), sonothermal treatment (ST), and pre-enzymatic sonothermal treatment (PEST) conditions: fructose, glucose, sucrose, and fructans were analyzed by HPLC and the total water soluble carbohydrates was determined. At 70 °C, the highest WSC values (482 mg/gd.m.) were obtained, with a fructan proportion of 69%. Pre-enzymatic treatment at 70 °C resulted in a high WSC content with the highest fructans proportion (87%) and lowest contents of RS and sucrose. The effect of the interaction between ultrasound and enzymatic treatments was limited by the high-temperature effect (70 °C), thereby minimizing the extraction. Microscopy analyses showed cell-wall modifications with the ST and PET treatments, which caused an increase in the total soluble sugars. The combination of enzymatic and sonothermal treatments at 70 °C resulted in the extraction of fructans in a higher yield and with less degradation. This circumvents the need for traditional high-energy processes, which could be beneficial for the extraction of WSC such as fructans from sotol or other economically important plants.
publishDate 2015
dc.date.none.fl_str_mv 2015-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13470
Pardo Rueda, Abril J.; Quintero Ramos, Armando; Genovese, Diego Bautista; Camacho Dávila, Alejandro; Zepeda Rodríguez, Armando; et al.; Efficient extraction of fructans from sotol plant (Dasylirion leiophyllum) enhanced by a combination of enzymatic and sonothermal treatments.; Elsevier Science; Food And Bioproducts Processing; 94; 4-2015; 398-404
0960-3085
url http://hdl.handle.net/11336/13470
identifier_str_mv Pardo Rueda, Abril J.; Quintero Ramos, Armando; Genovese, Diego Bautista; Camacho Dávila, Alejandro; Zepeda Rodríguez, Armando; et al.; Efficient extraction of fructans from sotol plant (Dasylirion leiophyllum) enhanced by a combination of enzymatic and sonothermal treatments.; Elsevier Science; Food And Bioproducts Processing; 94; 4-2015; 398-404
0960-3085
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S096030851400056X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fbp.2014.05.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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