New Insights into the Taxonomy of Malacopsylloidea Superfamily (Siphonaptera) Based on Morphological, Molecular and Phylogenetic Characterization of Phthiropsylla agenoris (Malacop...

Autores
Zurita, Antonio; Lareschi, Marcela; Cutillas, Cristina
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
From a phylogenetic point of view, the Malacopsyllidae family and the Rhopalopsillidae family (comprising Parapsyllinae and Rhopalopsyllinae subfamilies) have been traditionally classified within the Malacopsylloidea superfamily, mostly restricted to South America. The phylogenetic relationships and taxonomic status of Malacopsyllidae and Rhopalopsillidae have never been assessed since no molecular loci of Malacopsyllidae have been sequenced by any authors, and the phylogeny provided so far was not based on any sort of formal quantitative analysis of flea morphology. Based on these precedents, the objective of this study was to carry out a comparative phylogenetic, molecular and morphological study of two different species belonging to each family, Phthiropsylla agenoris (Malacopsylla) and Polygenis (Polygenis) rimatus (Rhopalopsyllidae, Rhopalopsyllinae). In this study, we demonstrated the usefulness of several morphological features as diagnostic characters to differentiate between P. (P.) rimatus and P. agenoris. Using molecular and phylogenetic data, we easily discriminated between the two taxa (P. agenoris and P. (P.) rimatus) by comparing both nuclear and mitochondrial markers. This fact proves the usefulness of ITS2, EF1−α, cox1, cytb and cox2 as molecular diagnostic markers to characterize and identify different Siphonaptera taxa. Additionally, the phylogenetic results confirm, for the first time, the monophyly of the Malacopsyllidae family and suggest a clear paraphyletic position of the Paraspsyllinae subfamily and, consequently, the Rhopalopsyllidae family.
Fil: Zurita, Antonio. Universidad de Sevilla. Facultad de Farmacia.; España
Fil: Lareschi, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Cutillas, Cristina. Universidad de Sevilla. Facultad de Farmacia.; España
Materia
MALACOPSYLLIDAE
PHYLOGENY
RHOPALOPSYLLIDAE
SIPHONAPTERA
TAXONOMY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/227292
Acceder
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network_name_str CONICET Digital (CONICET)
spelling New Insights into the Taxonomy of Malacopsylloidea Superfamily (Siphonaptera) Based on Morphological, Molecular and Phylogenetic Characterization of Phthiropsylla agenoris (Malacopsyllidae) and Polygenis (Polygenis) rimatus (Rhopalopsyllidae)Zurita, AntonioLareschi, MarcelaCutillas, CristinaMALACOPSYLLIDAEPHYLOGENYRHOPALOPSYLLIDAESIPHONAPTERATAXONOMYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1From a phylogenetic point of view, the Malacopsyllidae family and the Rhopalopsillidae family (comprising Parapsyllinae and Rhopalopsyllinae subfamilies) have been traditionally classified within the Malacopsylloidea superfamily, mostly restricted to South America. The phylogenetic relationships and taxonomic status of Malacopsyllidae and Rhopalopsillidae have never been assessed since no molecular loci of Malacopsyllidae have been sequenced by any authors, and the phylogeny provided so far was not based on any sort of formal quantitative analysis of flea morphology. Based on these precedents, the objective of this study was to carry out a comparative phylogenetic, molecular and morphological study of two different species belonging to each family, Phthiropsylla agenoris (Malacopsylla) and Polygenis (Polygenis) rimatus (Rhopalopsyllidae, Rhopalopsyllinae). In this study, we demonstrated the usefulness of several morphological features as diagnostic characters to differentiate between P. (P.) rimatus and P. agenoris. Using molecular and phylogenetic data, we easily discriminated between the two taxa (P. agenoris and P. (P.) rimatus) by comparing both nuclear and mitochondrial markers. This fact proves the usefulness of ITS2, EF1−α, cox1, cytb and cox2 as molecular diagnostic markers to characterize and identify different Siphonaptera taxa. Additionally, the phylogenetic results confirm, for the first time, the monophyly of the Malacopsyllidae family and suggest a clear paraphyletic position of the Paraspsyllinae subfamily and, consequently, the Rhopalopsyllidae family.Fil: Zurita, Antonio. Universidad de Sevilla. Facultad de Farmacia.; EspañaFil: Lareschi, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Cutillas, Cristina. Universidad de Sevilla. Facultad de Farmacia.; EspañaMultidisciplinary Digital Publishing Institute2023-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/227292Zurita, Antonio; Lareschi, Marcela; Cutillas, Cristina; New Insights into the Taxonomy of Malacopsylloidea Superfamily (Siphonaptera) Based on Morphological, Molecular and Phylogenetic Characterization of Phthiropsylla agenoris (Malacopsyllidae) and Polygenis (Polygenis) rimatus (Rhopalopsyllidae); Multidisciplinary Digital Publishing Institute; Diversity; 15; 2; 2-2023; 1-171424-2818CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1424-2818/15/2/308info:eu-repo/semantics/altIdentifier/doi/10.3390/d15020308info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:08Zoai:ri.conicet.gov.ar:11336/227292instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:08.99CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv New Insights into the Taxonomy of Malacopsylloidea Superfamily (Siphonaptera) Based on Morphological, Molecular and Phylogenetic Characterization of Phthiropsylla agenoris (Malacopsyllidae) and Polygenis (Polygenis) rimatus (Rhopalopsyllidae)
title New Insights into the Taxonomy of Malacopsylloidea Superfamily (Siphonaptera) Based on Morphological, Molecular and Phylogenetic Characterization of Phthiropsylla agenoris (Malacopsyllidae) and Polygenis (Polygenis) rimatus (Rhopalopsyllidae)
spellingShingle New Insights into the Taxonomy of Malacopsylloidea Superfamily (Siphonaptera) Based on Morphological, Molecular and Phylogenetic Characterization of Phthiropsylla agenoris (Malacopsyllidae) and Polygenis (Polygenis) rimatus (Rhopalopsyllidae)
Zurita, Antonio
MALACOPSYLLIDAE
PHYLOGENY
RHOPALOPSYLLIDAE
SIPHONAPTERA
TAXONOMY
title_short New Insights into the Taxonomy of Malacopsylloidea Superfamily (Siphonaptera) Based on Morphological, Molecular and Phylogenetic Characterization of Phthiropsylla agenoris (Malacopsyllidae) and Polygenis (Polygenis) rimatus (Rhopalopsyllidae)
title_full New Insights into the Taxonomy of Malacopsylloidea Superfamily (Siphonaptera) Based on Morphological, Molecular and Phylogenetic Characterization of Phthiropsylla agenoris (Malacopsyllidae) and Polygenis (Polygenis) rimatus (Rhopalopsyllidae)
title_fullStr New Insights into the Taxonomy of Malacopsylloidea Superfamily (Siphonaptera) Based on Morphological, Molecular and Phylogenetic Characterization of Phthiropsylla agenoris (Malacopsyllidae) and Polygenis (Polygenis) rimatus (Rhopalopsyllidae)
title_full_unstemmed New Insights into the Taxonomy of Malacopsylloidea Superfamily (Siphonaptera) Based on Morphological, Molecular and Phylogenetic Characterization of Phthiropsylla agenoris (Malacopsyllidae) and Polygenis (Polygenis) rimatus (Rhopalopsyllidae)
title_sort New Insights into the Taxonomy of Malacopsylloidea Superfamily (Siphonaptera) Based on Morphological, Molecular and Phylogenetic Characterization of Phthiropsylla agenoris (Malacopsyllidae) and Polygenis (Polygenis) rimatus (Rhopalopsyllidae)
dc.creator.none.fl_str_mv Zurita, Antonio
Lareschi, Marcela
Cutillas, Cristina
author Zurita, Antonio
author_facet Zurita, Antonio
Lareschi, Marcela
Cutillas, Cristina
author_role author
author2 Lareschi, Marcela
Cutillas, Cristina
author2_role author
author
dc.subject.none.fl_str_mv MALACOPSYLLIDAE
PHYLOGENY
RHOPALOPSYLLIDAE
SIPHONAPTERA
TAXONOMY
topic MALACOPSYLLIDAE
PHYLOGENY
RHOPALOPSYLLIDAE
SIPHONAPTERA
TAXONOMY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv From a phylogenetic point of view, the Malacopsyllidae family and the Rhopalopsillidae family (comprising Parapsyllinae and Rhopalopsyllinae subfamilies) have been traditionally classified within the Malacopsylloidea superfamily, mostly restricted to South America. The phylogenetic relationships and taxonomic status of Malacopsyllidae and Rhopalopsillidae have never been assessed since no molecular loci of Malacopsyllidae have been sequenced by any authors, and the phylogeny provided so far was not based on any sort of formal quantitative analysis of flea morphology. Based on these precedents, the objective of this study was to carry out a comparative phylogenetic, molecular and morphological study of two different species belonging to each family, Phthiropsylla agenoris (Malacopsylla) and Polygenis (Polygenis) rimatus (Rhopalopsyllidae, Rhopalopsyllinae). In this study, we demonstrated the usefulness of several morphological features as diagnostic characters to differentiate between P. (P.) rimatus and P. agenoris. Using molecular and phylogenetic data, we easily discriminated between the two taxa (P. agenoris and P. (P.) rimatus) by comparing both nuclear and mitochondrial markers. This fact proves the usefulness of ITS2, EF1−α, cox1, cytb and cox2 as molecular diagnostic markers to characterize and identify different Siphonaptera taxa. Additionally, the phylogenetic results confirm, for the first time, the monophyly of the Malacopsyllidae family and suggest a clear paraphyletic position of the Paraspsyllinae subfamily and, consequently, the Rhopalopsyllidae family.
Fil: Zurita, Antonio. Universidad de Sevilla. Facultad de Farmacia.; España
Fil: Lareschi, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Cutillas, Cristina. Universidad de Sevilla. Facultad de Farmacia.; España
description From a phylogenetic point of view, the Malacopsyllidae family and the Rhopalopsillidae family (comprising Parapsyllinae and Rhopalopsyllinae subfamilies) have been traditionally classified within the Malacopsylloidea superfamily, mostly restricted to South America. The phylogenetic relationships and taxonomic status of Malacopsyllidae and Rhopalopsillidae have never been assessed since no molecular loci of Malacopsyllidae have been sequenced by any authors, and the phylogeny provided so far was not based on any sort of formal quantitative analysis of flea morphology. Based on these precedents, the objective of this study was to carry out a comparative phylogenetic, molecular and morphological study of two different species belonging to each family, Phthiropsylla agenoris (Malacopsylla) and Polygenis (Polygenis) rimatus (Rhopalopsyllidae, Rhopalopsyllinae). In this study, we demonstrated the usefulness of several morphological features as diagnostic characters to differentiate between P. (P.) rimatus and P. agenoris. Using molecular and phylogenetic data, we easily discriminated between the two taxa (P. agenoris and P. (P.) rimatus) by comparing both nuclear and mitochondrial markers. This fact proves the usefulness of ITS2, EF1−α, cox1, cytb and cox2 as molecular diagnostic markers to characterize and identify different Siphonaptera taxa. Additionally, the phylogenetic results confirm, for the first time, the monophyly of the Malacopsyllidae family and suggest a clear paraphyletic position of the Paraspsyllinae subfamily and, consequently, the Rhopalopsyllidae family.
publishDate 2023
dc.date.none.fl_str_mv 2023-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/227292
Zurita, Antonio; Lareschi, Marcela; Cutillas, Cristina; New Insights into the Taxonomy of Malacopsylloidea Superfamily (Siphonaptera) Based on Morphological, Molecular and Phylogenetic Characterization of Phthiropsylla agenoris (Malacopsyllidae) and Polygenis (Polygenis) rimatus (Rhopalopsyllidae); Multidisciplinary Digital Publishing Institute; Diversity; 15; 2; 2-2023; 1-17
1424-2818
CONICET Digital
CONICET
url http://hdl.handle.net/11336/227292
identifier_str_mv Zurita, Antonio; Lareschi, Marcela; Cutillas, Cristina; New Insights into the Taxonomy of Malacopsylloidea Superfamily (Siphonaptera) Based on Morphological, Molecular and Phylogenetic Characterization of Phthiropsylla agenoris (Malacopsyllidae) and Polygenis (Polygenis) rimatus (Rhopalopsyllidae); Multidisciplinary Digital Publishing Institute; Diversity; 15; 2; 2-2023; 1-17
1424-2818
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1424-2818/15/2/308
info:eu-repo/semantics/altIdentifier/doi/10.3390/d15020308
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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