Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin
- Autores
- Rodríguez, Ernesto; Carasi, Paula; Frigerio, Sofía; da Costa, Valeria; van Vliet, Sandra; Noya, Verónica; Brossard, Natalie; van Kooyk, Yvette; García Vallejo, Juan J.; Freire, Teresa
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fasciolosis, caused by Fasciola hepatica and Fasciola gigantica, is a trematode zoonosis of interest in public health and livestock production. Like other helminths, F. hepatica modulates the host immune response by inducing potent polarized Th2 and regulatory T cell immune responses and by downregulating the production of Th1 cytokines. In this work, we show that F. hepatica glycans increase Th2 immune responses by immunomodulating TLR-induced maturation and function of dendritic cells (DCs). This process was mediated by the macrophage Gal/GalNAc lectin (MGL) expressed on DCs, which recognizes the Tn antigen (GalNAc-Ser/Thr) on parasite components. More interestingly, we identified MGL-expressing CD11c+ cells in infected animals and showed that these cells are recruited both to the peritoneum and the liver upon F. hepatica infection. These cells express the regulatory cytokines IL-10, TNFα and TGFβ and a variety of regulatory markers. Furthermore, MGL+ CD11c+ cells expand parasite-specific Th2/regulatory cells and suppress Th1 polarization. The results presented here suggest a potential role of MGL in the immunomodulation of DCs induced by F. hepatica and contribute to a better understanding of the molecular and immunoregulatory mechanisms induced by this parasite.
Fil: Rodríguez, Ernesto. Universidad de la República; Uruguay
Fil: Carasi, Paula. Universidad de la República; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Frigerio, Sofía. Universidad de la República; Uruguay
Fil: da Costa, Valeria. Universidad de la República; Uruguay
Fil: van Vliet, Sandra. VU University Medical Center; Países Bajos
Fil: Noya, Verónica. Universidad de la República; Uruguay
Fil: Brossard, Natalie. Universidad de la República; Uruguay
Fil: van Kooyk, Yvette. VU University Medical Center; Países Bajos
Fil: García Vallejo, Juan J.. VU University Medical Center; Países Bajos
Fil: Freire, Teresa. Universidad de la República; Uruguay - Materia
-
C-TYPE LECTIN RECEPTORS
DENDRITIC CELL
GLYCANS
HELMINTH
IMMUNE REGULATION
MACROPHAGE GAL/GALNAC LECTIN - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/56479
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/56479 |
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CONICET Digital (CONICET) |
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Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectinRodríguez, ErnestoCarasi, PaulaFrigerio, Sofíada Costa, Valeriavan Vliet, SandraNoya, VerónicaBrossard, Natalievan Kooyk, YvetteGarcía Vallejo, Juan J.Freire, TeresaC-TYPE LECTIN RECEPTORSDENDRITIC CELLGLYCANSHELMINTHIMMUNE REGULATIONMACROPHAGE GAL/GALNAC LECTINhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Fasciolosis, caused by Fasciola hepatica and Fasciola gigantica, is a trematode zoonosis of interest in public health and livestock production. Like other helminths, F. hepatica modulates the host immune response by inducing potent polarized Th2 and regulatory T cell immune responses and by downregulating the production of Th1 cytokines. In this work, we show that F. hepatica glycans increase Th2 immune responses by immunomodulating TLR-induced maturation and function of dendritic cells (DCs). This process was mediated by the macrophage Gal/GalNAc lectin (MGL) expressed on DCs, which recognizes the Tn antigen (GalNAc-Ser/Thr) on parasite components. More interestingly, we identified MGL-expressing CD11c+ cells in infected animals and showed that these cells are recruited both to the peritoneum and the liver upon F. hepatica infection. These cells express the regulatory cytokines IL-10, TNFα and TGFβ and a variety of regulatory markers. Furthermore, MGL+ CD11c+ cells expand parasite-specific Th2/regulatory cells and suppress Th1 polarization. The results presented here suggest a potential role of MGL in the immunomodulation of DCs induced by F. hepatica and contribute to a better understanding of the molecular and immunoregulatory mechanisms induced by this parasite.Fil: Rodríguez, Ernesto. Universidad de la República; UruguayFil: Carasi, Paula. Universidad de la República; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Frigerio, Sofía. Universidad de la República; UruguayFil: da Costa, Valeria. Universidad de la República; UruguayFil: van Vliet, Sandra. VU University Medical Center; Países BajosFil: Noya, Verónica. Universidad de la República; UruguayFil: Brossard, Natalie. Universidad de la República; UruguayFil: van Kooyk, Yvette. VU University Medical Center; Países BajosFil: García Vallejo, Juan J.. VU University Medical Center; Países BajosFil: Freire, Teresa. Universidad de la República; UruguayFrontiers Research Foundation2017-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/56479Rodríguez, Ernesto; Carasi, Paula; Frigerio, Sofía; da Costa, Valeria; van Vliet, Sandra; et al.; Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin; Frontiers Research Foundation; Frontiers in Immunology; 8; MAR; 3-2017; 1-161664-3224CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2017.00264info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2017.00264/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:48Zoai:ri.conicet.gov.ar:11336/56479instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:48.507CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin |
title |
Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin |
spellingShingle |
Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin Rodríguez, Ernesto C-TYPE LECTIN RECEPTORS DENDRITIC CELL GLYCANS HELMINTH IMMUNE REGULATION MACROPHAGE GAL/GALNAC LECTIN |
title_short |
Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin |
title_full |
Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin |
title_fullStr |
Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin |
title_full_unstemmed |
Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin |
title_sort |
Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin |
dc.creator.none.fl_str_mv |
Rodríguez, Ernesto Carasi, Paula Frigerio, Sofía da Costa, Valeria van Vliet, Sandra Noya, Verónica Brossard, Natalie van Kooyk, Yvette García Vallejo, Juan J. Freire, Teresa |
author |
Rodríguez, Ernesto |
author_facet |
Rodríguez, Ernesto Carasi, Paula Frigerio, Sofía da Costa, Valeria van Vliet, Sandra Noya, Verónica Brossard, Natalie van Kooyk, Yvette García Vallejo, Juan J. Freire, Teresa |
author_role |
author |
author2 |
Carasi, Paula Frigerio, Sofía da Costa, Valeria van Vliet, Sandra Noya, Verónica Brossard, Natalie van Kooyk, Yvette García Vallejo, Juan J. Freire, Teresa |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
C-TYPE LECTIN RECEPTORS DENDRITIC CELL GLYCANS HELMINTH IMMUNE REGULATION MACROPHAGE GAL/GALNAC LECTIN |
topic |
C-TYPE LECTIN RECEPTORS DENDRITIC CELL GLYCANS HELMINTH IMMUNE REGULATION MACROPHAGE GAL/GALNAC LECTIN |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Fasciolosis, caused by Fasciola hepatica and Fasciola gigantica, is a trematode zoonosis of interest in public health and livestock production. Like other helminths, F. hepatica modulates the host immune response by inducing potent polarized Th2 and regulatory T cell immune responses and by downregulating the production of Th1 cytokines. In this work, we show that F. hepatica glycans increase Th2 immune responses by immunomodulating TLR-induced maturation and function of dendritic cells (DCs). This process was mediated by the macrophage Gal/GalNAc lectin (MGL) expressed on DCs, which recognizes the Tn antigen (GalNAc-Ser/Thr) on parasite components. More interestingly, we identified MGL-expressing CD11c+ cells in infected animals and showed that these cells are recruited both to the peritoneum and the liver upon F. hepatica infection. These cells express the regulatory cytokines IL-10, TNFα and TGFβ and a variety of regulatory markers. Furthermore, MGL+ CD11c+ cells expand parasite-specific Th2/regulatory cells and suppress Th1 polarization. The results presented here suggest a potential role of MGL in the immunomodulation of DCs induced by F. hepatica and contribute to a better understanding of the molecular and immunoregulatory mechanisms induced by this parasite. Fil: Rodríguez, Ernesto. Universidad de la República; Uruguay Fil: Carasi, Paula. Universidad de la República; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Frigerio, Sofía. Universidad de la República; Uruguay Fil: da Costa, Valeria. Universidad de la República; Uruguay Fil: van Vliet, Sandra. VU University Medical Center; Países Bajos Fil: Noya, Verónica. Universidad de la República; Uruguay Fil: Brossard, Natalie. Universidad de la República; Uruguay Fil: van Kooyk, Yvette. VU University Medical Center; Países Bajos Fil: García Vallejo, Juan J.. VU University Medical Center; Países Bajos Fil: Freire, Teresa. Universidad de la República; Uruguay |
description |
Fasciolosis, caused by Fasciola hepatica and Fasciola gigantica, is a trematode zoonosis of interest in public health and livestock production. Like other helminths, F. hepatica modulates the host immune response by inducing potent polarized Th2 and regulatory T cell immune responses and by downregulating the production of Th1 cytokines. In this work, we show that F. hepatica glycans increase Th2 immune responses by immunomodulating TLR-induced maturation and function of dendritic cells (DCs). This process was mediated by the macrophage Gal/GalNAc lectin (MGL) expressed on DCs, which recognizes the Tn antigen (GalNAc-Ser/Thr) on parasite components. More interestingly, we identified MGL-expressing CD11c+ cells in infected animals and showed that these cells are recruited both to the peritoneum and the liver upon F. hepatica infection. These cells express the regulatory cytokines IL-10, TNFα and TGFβ and a variety of regulatory markers. Furthermore, MGL+ CD11c+ cells expand parasite-specific Th2/regulatory cells and suppress Th1 polarization. The results presented here suggest a potential role of MGL in the immunomodulation of DCs induced by F. hepatica and contribute to a better understanding of the molecular and immunoregulatory mechanisms induced by this parasite. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/56479 Rodríguez, Ernesto; Carasi, Paula; Frigerio, Sofía; da Costa, Valeria; van Vliet, Sandra; et al.; Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin; Frontiers Research Foundation; Frontiers in Immunology; 8; MAR; 3-2017; 1-16 1664-3224 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/56479 |
identifier_str_mv |
Rodríguez, Ernesto; Carasi, Paula; Frigerio, Sofía; da Costa, Valeria; van Vliet, Sandra; et al.; Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin; Frontiers Research Foundation; Frontiers in Immunology; 8; MAR; 3-2017; 1-16 1664-3224 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2017.00264 info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2017.00264/full |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Research Foundation |
publisher.none.fl_str_mv |
Frontiers Research Foundation |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842270171029307392 |
score |
13.13397 |