Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin

Autores
Rodríguez, Ernesto; Carasi, Paula; Frigerio, Sofía; da Costa, Valeria; van Vliet, Sandra; Noya, Verónica; Brossard, Natalie; van Kooyk, Yvette; García Vallejo, Juan J.; Freire, Teresa
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fasciolosis, caused by Fasciola hepatica and Fasciola gigantica, is a trematode zoonosis of interest in public health and livestock production. Like other helminths, F. hepatica modulates the host immune response by inducing potent polarized Th2 and regulatory T cell immune responses and by downregulating the production of Th1 cytokines. In this work, we show that F. hepatica glycans increase Th2 immune responses by immunomodulating TLR-induced maturation and function of dendritic cells (DCs). This process was mediated by the macrophage Gal/GalNAc lectin (MGL) expressed on DCs, which recognizes the Tn antigen (GalNAc-Ser/Thr) on parasite components. More interestingly, we identified MGL-expressing CD11c+ cells in infected animals and showed that these cells are recruited both to the peritoneum and the liver upon F. hepatica infection. These cells express the regulatory cytokines IL-10, TNFα and TGFβ and a variety of regulatory markers. Furthermore, MGL+ CD11c+ cells expand parasite-specific Th2/regulatory cells and suppress Th1 polarization. The results presented here suggest a potential role of MGL in the immunomodulation of DCs induced by F. hepatica and contribute to a better understanding of the molecular and immunoregulatory mechanisms induced by this parasite.
Fil: Rodríguez, Ernesto. Universidad de la República; Uruguay
Fil: Carasi, Paula. Universidad de la República; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Frigerio, Sofía. Universidad de la República; Uruguay
Fil: da Costa, Valeria. Universidad de la República; Uruguay
Fil: van Vliet, Sandra. VU University Medical Center; Países Bajos
Fil: Noya, Verónica. Universidad de la República; Uruguay
Fil: Brossard, Natalie. Universidad de la República; Uruguay
Fil: van Kooyk, Yvette. VU University Medical Center; Países Bajos
Fil: García Vallejo, Juan J.. VU University Medical Center; Países Bajos
Fil: Freire, Teresa. Universidad de la República; Uruguay
Materia
C-TYPE LECTIN RECEPTORS
DENDRITIC CELL
GLYCANS
HELMINTH
IMMUNE REGULATION
MACROPHAGE GAL/GALNAC LECTIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/56479
Acceder
id CONICETDig_c2c05e0de3827ef6f512c5863503296c
oai_identifier_str oai:ri.conicet.gov.ar:11336/56479
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectinRodríguez, ErnestoCarasi, PaulaFrigerio, Sofíada Costa, Valeriavan Vliet, SandraNoya, VerónicaBrossard, Natalievan Kooyk, YvetteGarcía Vallejo, Juan J.Freire, TeresaC-TYPE LECTIN RECEPTORSDENDRITIC CELLGLYCANSHELMINTHIMMUNE REGULATIONMACROPHAGE GAL/GALNAC LECTINhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Fasciolosis, caused by Fasciola hepatica and Fasciola gigantica, is a trematode zoonosis of interest in public health and livestock production. Like other helminths, F. hepatica modulates the host immune response by inducing potent polarized Th2 and regulatory T cell immune responses and by downregulating the production of Th1 cytokines. In this work, we show that F. hepatica glycans increase Th2 immune responses by immunomodulating TLR-induced maturation and function of dendritic cells (DCs). This process was mediated by the macrophage Gal/GalNAc lectin (MGL) expressed on DCs, which recognizes the Tn antigen (GalNAc-Ser/Thr) on parasite components. More interestingly, we identified MGL-expressing CD11c+ cells in infected animals and showed that these cells are recruited both to the peritoneum and the liver upon F. hepatica infection. These cells express the regulatory cytokines IL-10, TNFα and TGFβ and a variety of regulatory markers. Furthermore, MGL+ CD11c+ cells expand parasite-specific Th2/regulatory cells and suppress Th1 polarization. The results presented here suggest a potential role of MGL in the immunomodulation of DCs induced by F. hepatica and contribute to a better understanding of the molecular and immunoregulatory mechanisms induced by this parasite.Fil: Rodríguez, Ernesto. Universidad de la República; UruguayFil: Carasi, Paula. Universidad de la República; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Frigerio, Sofía. Universidad de la República; UruguayFil: da Costa, Valeria. Universidad de la República; UruguayFil: van Vliet, Sandra. VU University Medical Center; Países BajosFil: Noya, Verónica. Universidad de la República; UruguayFil: Brossard, Natalie. Universidad de la República; UruguayFil: van Kooyk, Yvette. VU University Medical Center; Países BajosFil: García Vallejo, Juan J.. VU University Medical Center; Países BajosFil: Freire, Teresa. Universidad de la República; UruguayFrontiers Research Foundation2017-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/56479Rodríguez, Ernesto; Carasi, Paula; Frigerio, Sofía; da Costa, Valeria; van Vliet, Sandra; et al.; Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin; Frontiers Research Foundation; Frontiers in Immunology; 8; MAR; 3-2017; 1-161664-3224CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2017.00264info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2017.00264/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T10:00:22Zoai:ri.conicet.gov.ar:11336/56479instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 10:00:22.953CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin
title Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin
spellingShingle Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin
Rodríguez, Ernesto
C-TYPE LECTIN RECEPTORS
DENDRITIC CELL
GLYCANS
HELMINTH
IMMUNE REGULATION
MACROPHAGE GAL/GALNAC LECTIN
title_short Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin
title_full Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin
title_fullStr Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin
title_full_unstemmed Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin
title_sort Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin
dc.creator.none.fl_str_mv Rodríguez, Ernesto
Carasi, Paula
Frigerio, Sofía
da Costa, Valeria
van Vliet, Sandra
Noya, Verónica
Brossard, Natalie
van Kooyk, Yvette
García Vallejo, Juan J.
Freire, Teresa
author Rodríguez, Ernesto
author_facet Rodríguez, Ernesto
Carasi, Paula
Frigerio, Sofía
da Costa, Valeria
van Vliet, Sandra
Noya, Verónica
Brossard, Natalie
van Kooyk, Yvette
García Vallejo, Juan J.
Freire, Teresa
author_role author
author2 Carasi, Paula
Frigerio, Sofía
da Costa, Valeria
van Vliet, Sandra
Noya, Verónica
Brossard, Natalie
van Kooyk, Yvette
García Vallejo, Juan J.
Freire, Teresa
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv C-TYPE LECTIN RECEPTORS
DENDRITIC CELL
GLYCANS
HELMINTH
IMMUNE REGULATION
MACROPHAGE GAL/GALNAC LECTIN
topic C-TYPE LECTIN RECEPTORS
DENDRITIC CELL
GLYCANS
HELMINTH
IMMUNE REGULATION
MACROPHAGE GAL/GALNAC LECTIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Fasciolosis, caused by Fasciola hepatica and Fasciola gigantica, is a trematode zoonosis of interest in public health and livestock production. Like other helminths, F. hepatica modulates the host immune response by inducing potent polarized Th2 and regulatory T cell immune responses and by downregulating the production of Th1 cytokines. In this work, we show that F. hepatica glycans increase Th2 immune responses by immunomodulating TLR-induced maturation and function of dendritic cells (DCs). This process was mediated by the macrophage Gal/GalNAc lectin (MGL) expressed on DCs, which recognizes the Tn antigen (GalNAc-Ser/Thr) on parasite components. More interestingly, we identified MGL-expressing CD11c+ cells in infected animals and showed that these cells are recruited both to the peritoneum and the liver upon F. hepatica infection. These cells express the regulatory cytokines IL-10, TNFα and TGFβ and a variety of regulatory markers. Furthermore, MGL+ CD11c+ cells expand parasite-specific Th2/regulatory cells and suppress Th1 polarization. The results presented here suggest a potential role of MGL in the immunomodulation of DCs induced by F. hepatica and contribute to a better understanding of the molecular and immunoregulatory mechanisms induced by this parasite.
Fil: Rodríguez, Ernesto. Universidad de la República; Uruguay
Fil: Carasi, Paula. Universidad de la República; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Frigerio, Sofía. Universidad de la República; Uruguay
Fil: da Costa, Valeria. Universidad de la República; Uruguay
Fil: van Vliet, Sandra. VU University Medical Center; Países Bajos
Fil: Noya, Verónica. Universidad de la República; Uruguay
Fil: Brossard, Natalie. Universidad de la República; Uruguay
Fil: van Kooyk, Yvette. VU University Medical Center; Países Bajos
Fil: García Vallejo, Juan J.. VU University Medical Center; Países Bajos
Fil: Freire, Teresa. Universidad de la República; Uruguay
description Fasciolosis, caused by Fasciola hepatica and Fasciola gigantica, is a trematode zoonosis of interest in public health and livestock production. Like other helminths, F. hepatica modulates the host immune response by inducing potent polarized Th2 and regulatory T cell immune responses and by downregulating the production of Th1 cytokines. In this work, we show that F. hepatica glycans increase Th2 immune responses by immunomodulating TLR-induced maturation and function of dendritic cells (DCs). This process was mediated by the macrophage Gal/GalNAc lectin (MGL) expressed on DCs, which recognizes the Tn antigen (GalNAc-Ser/Thr) on parasite components. More interestingly, we identified MGL-expressing CD11c+ cells in infected animals and showed that these cells are recruited both to the peritoneum and the liver upon F. hepatica infection. These cells express the regulatory cytokines IL-10, TNFα and TGFβ and a variety of regulatory markers. Furthermore, MGL+ CD11c+ cells expand parasite-specific Th2/regulatory cells and suppress Th1 polarization. The results presented here suggest a potential role of MGL in the immunomodulation of DCs induced by F. hepatica and contribute to a better understanding of the molecular and immunoregulatory mechanisms induced by this parasite.
publishDate 2017
dc.date.none.fl_str_mv 2017-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/56479
Rodríguez, Ernesto; Carasi, Paula; Frigerio, Sofía; da Costa, Valeria; van Vliet, Sandra; et al.; Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin; Frontiers Research Foundation; Frontiers in Immunology; 8; MAR; 3-2017; 1-16
1664-3224
CONICET Digital
CONICET
url http://hdl.handle.net/11336/56479
identifier_str_mv Rodríguez, Ernesto; Carasi, Paula; Frigerio, Sofía; da Costa, Valeria; van Vliet, Sandra; et al.; Fasciola hepatica immune regulates CD11c+ cells by interacting with the macrophage gal/GalNAc lectin; Frontiers Research Foundation; Frontiers in Immunology; 8; MAR; 3-2017; 1-16
1664-3224
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2017.00264
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2017.00264/full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Research Foundation
publisher.none.fl_str_mv Frontiers Research Foundation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.6313505

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