PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium meliloti

Autores
Sorroche, Fernando Guido; Giordano, Walter Fabian
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Exopolysaccharide (EPS) production by the rhizobacterium Sinorhizobium meliloti is essential for root nodule formation on its legume host (alfalfa), and for establishment of a nitrogen-fixing symbiosis between the two partners. Production of EPS II (galactoglucan) by certain S. meliloti strains results in a mucoid colony phenotype. Other strains that are unable to produce EPS II display a dry phenotype, due to the presence of an insertion element in the gene expR, a key regulator involved in many important cellular processes, including production of low-molecular-weight EPS II. We describe a series of three programmed undergraduate biochemistry laboratory classes teaching PCR and electrophoresis procedures to detect non-functional expR loci in S. meliloti.
Fil: Sorroche, Fernando Guido. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Giordano, Walter Fabian. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Materia
EXOPOLYSACCHARIDES
EXPR GEN
PCR
RHIZOBACTERIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/189254
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium melilotiSorroche, Fernando GuidoGiordano, Walter FabianEXOPOLYSACCHARIDESEXPR GENPCRRHIZOBACTERIAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Exopolysaccharide (EPS) production by the rhizobacterium Sinorhizobium meliloti is essential for root nodule formation on its legume host (alfalfa), and for establishment of a nitrogen-fixing symbiosis between the two partners. Production of EPS II (galactoglucan) by certain S. meliloti strains results in a mucoid colony phenotype. Other strains that are unable to produce EPS II display a dry phenotype, due to the presence of an insertion element in the gene expR, a key regulator involved in many important cellular processes, including production of low-molecular-weight EPS II. We describe a series of three programmed undergraduate biochemistry laboratory classes teaching PCR and electrophoresis procedures to detect non-functional expR loci in S. meliloti.Fil: Sorroche, Fernando Guido. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Giordano, Walter Fabian. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaJohn Wiley & Sons2012-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189254Sorroche, Fernando Guido; Giordano, Walter Fabian; PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium meliloti; John Wiley & Sons; Biochemistry and Molecular Biology Education; 40; 2; 4-2012; 108-1111470-8175CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://iubmb.onlinelibrary.wiley.com/doi/10.1002/bmb.20560info:eu-repo/semantics/altIdentifier/doi/10.1002/bmb.20560info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:52:56Zoai:ri.conicet.gov.ar:11336/189254instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:52:57.059CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium meliloti
title PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium meliloti
spellingShingle PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium meliloti
Sorroche, Fernando Guido
EXOPOLYSACCHARIDES
EXPR GEN
PCR
RHIZOBACTERIA
title_short PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium meliloti
title_full PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium meliloti
title_fullStr PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium meliloti
title_full_unstemmed PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium meliloti
title_sort PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium meliloti
dc.creator.none.fl_str_mv Sorroche, Fernando Guido
Giordano, Walter Fabian
author Sorroche, Fernando Guido
author_facet Sorroche, Fernando Guido
Giordano, Walter Fabian
author_role author
author2 Giordano, Walter Fabian
author2_role author
dc.subject.none.fl_str_mv EXOPOLYSACCHARIDES
EXPR GEN
PCR
RHIZOBACTERIA
topic EXOPOLYSACCHARIDES
EXPR GEN
PCR
RHIZOBACTERIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Exopolysaccharide (EPS) production by the rhizobacterium Sinorhizobium meliloti is essential for root nodule formation on its legume host (alfalfa), and for establishment of a nitrogen-fixing symbiosis between the two partners. Production of EPS II (galactoglucan) by certain S. meliloti strains results in a mucoid colony phenotype. Other strains that are unable to produce EPS II display a dry phenotype, due to the presence of an insertion element in the gene expR, a key regulator involved in many important cellular processes, including production of low-molecular-weight EPS II. We describe a series of three programmed undergraduate biochemistry laboratory classes teaching PCR and electrophoresis procedures to detect non-functional expR loci in S. meliloti.
Fil: Sorroche, Fernando Guido. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Giordano, Walter Fabian. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
description Exopolysaccharide (EPS) production by the rhizobacterium Sinorhizobium meliloti is essential for root nodule formation on its legume host (alfalfa), and for establishment of a nitrogen-fixing symbiosis between the two partners. Production of EPS II (galactoglucan) by certain S. meliloti strains results in a mucoid colony phenotype. Other strains that are unable to produce EPS II display a dry phenotype, due to the presence of an insertion element in the gene expR, a key regulator involved in many important cellular processes, including production of low-molecular-weight EPS II. We describe a series of three programmed undergraduate biochemistry laboratory classes teaching PCR and electrophoresis procedures to detect non-functional expR loci in S. meliloti.
publishDate 2012
dc.date.none.fl_str_mv 2012-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/189254
Sorroche, Fernando Guido; Giordano, Walter Fabian; PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium meliloti; John Wiley & Sons; Biochemistry and Molecular Biology Education; 40; 2; 4-2012; 108-111
1470-8175
CONICET Digital
CONICET
url http://hdl.handle.net/11336/189254
identifier_str_mv Sorroche, Fernando Guido; Giordano, Walter Fabian; PCR Analysis of expR Gene Regulating Biosynthesis of Exopolysaccharides in Sinorhizobium meliloti; John Wiley & Sons; Biochemistry and Molecular Biology Education; 40; 2; 4-2012; 108-111
1470-8175
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://iubmb.onlinelibrary.wiley.com/doi/10.1002/bmb.20560
info:eu-repo/semantics/altIdentifier/doi/10.1002/bmb.20560
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24909

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