Sex chromosomes in the Vizcacheras’ White-lipped frog, Leptodactylus bufonius (Anura, Leptodactylidae)

Autores
Schneider, Rosio Gabriela; Ferro, Juan Martín; Reinko, Ivana Noelia; Boeris, Juan Martín; Cardozo, Dario Elbio; Baldo, Juan Diego
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cytogenetic analyses were performed on specimens of Leptodactylus bufonius from different localities in Argentina. Mitotic chromosomes were studied with Giemsa and differential staining techniques (Ag-NOR, C-banding, and CMA3 /DAPI) and fl uorescence in situ hybridization with the 18S DNAr probe. All specimens showed karyotypes with 2n = 2x = 22 and FN = 44. Secondary constrictions were present in the long arm of chromosome pair 8, coincident with Ag-NOR and hybridization signals of the 18S DNAr probe. The C-banding technique evidenced an important amount of heterochromatin with a sex-linked pericentromeric band in the short arm of chromosome pair 4. This heterochromatic band was heteromorphic in males but present in both homologues of females, and it was CMA3 positive (DAPI negative) at fl uorescence staining. The occurrence of heteromorphic XY sex chromosomes in L. bufonius is the second known case in Leptodactylus and the fi fth within the speciose family Leptodactylidae.
Fil: Schneider, Rosio Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Fil: Ferro, Juan Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Fil: Reinko, Ivana Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Fil: Boeris, Juan Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Fil: Cardozo, Dario Elbio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Fil: Baldo, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Materia
C-BANDING
CYTOGENETICS
HETEROCHROMATIN
HETEROMORPHISM
XY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/157055
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/157055
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sex chromosomes in the Vizcacheras’ White-lipped frog, Leptodactylus bufonius (Anura, Leptodactylidae)Schneider, Rosio GabrielaFerro, Juan MartínReinko, Ivana NoeliaBoeris, Juan MartínCardozo, Dario ElbioBaldo, Juan DiegoC-BANDINGCYTOGENETICSHETEROCHROMATINHETEROMORPHISMXYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cytogenetic analyses were performed on specimens of Leptodactylus bufonius from different localities in Argentina. Mitotic chromosomes were studied with Giemsa and differential staining techniques (Ag-NOR, C-banding, and CMA3 /DAPI) and fl uorescence in situ hybridization with the 18S DNAr probe. All specimens showed karyotypes with 2n = 2x = 22 and FN = 44. Secondary constrictions were present in the long arm of chromosome pair 8, coincident with Ag-NOR and hybridization signals of the 18S DNAr probe. The C-banding technique evidenced an important amount of heterochromatin with a sex-linked pericentromeric band in the short arm of chromosome pair 4. This heterochromatic band was heteromorphic in males but present in both homologues of females, and it was CMA3 positive (DAPI negative) at fl uorescence staining. The occurrence of heteromorphic XY sex chromosomes in L. bufonius is the second known case in Leptodactylus and the fi fth within the speciose family Leptodactylidae.Fil: Schneider, Rosio Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; ArgentinaFil: Ferro, Juan Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; ArgentinaFil: Reinko, Ivana Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; ArgentinaFil: Boeris, Juan Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; ArgentinaFil: Cardozo, Dario Elbio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; ArgentinaFil: Baldo, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; ArgentinaAcademia Brasilera de Ciencias2021-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/157055Schneider, Rosio Gabriela; Ferro, Juan Martín; Reinko, Ivana Noelia; Boeris, Juan Martín; Cardozo, Dario Elbio; et al.; Sex chromosomes in the Vizcacheras’ White-lipped frog, Leptodactylus bufonius (Anura, Leptodactylidae); Academia Brasilera de Ciencias; Anais da Academia Brasileira de Ciências; 93; 2; 6-2021; 1-120001-37651678-2690CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1590/0001-3765202120190426info:eu-repo/semantics/altIdentifier/url/http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000301018&tlng=eninfo:eu-repo/semantics/altIdentifier/url/https://www.ipni.org/p/3817-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:38:12Zoai:ri.conicet.gov.ar:11336/157055instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:38:13.09CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sex chromosomes in the Vizcacheras’ White-lipped frog, Leptodactylus bufonius (Anura, Leptodactylidae)
title Sex chromosomes in the Vizcacheras’ White-lipped frog, Leptodactylus bufonius (Anura, Leptodactylidae)
spellingShingle Sex chromosomes in the Vizcacheras’ White-lipped frog, Leptodactylus bufonius (Anura, Leptodactylidae)
Schneider, Rosio Gabriela
C-BANDING
CYTOGENETICS
HETEROCHROMATIN
HETEROMORPHISM
XY
title_short Sex chromosomes in the Vizcacheras’ White-lipped frog, Leptodactylus bufonius (Anura, Leptodactylidae)
title_full Sex chromosomes in the Vizcacheras’ White-lipped frog, Leptodactylus bufonius (Anura, Leptodactylidae)
title_fullStr Sex chromosomes in the Vizcacheras’ White-lipped frog, Leptodactylus bufonius (Anura, Leptodactylidae)
title_full_unstemmed Sex chromosomes in the Vizcacheras’ White-lipped frog, Leptodactylus bufonius (Anura, Leptodactylidae)
title_sort Sex chromosomes in the Vizcacheras’ White-lipped frog, Leptodactylus bufonius (Anura, Leptodactylidae)
dc.creator.none.fl_str_mv Schneider, Rosio Gabriela
Ferro, Juan Martín
Reinko, Ivana Noelia
Boeris, Juan Martín
Cardozo, Dario Elbio
Baldo, Juan Diego
author Schneider, Rosio Gabriela
author_facet Schneider, Rosio Gabriela
Ferro, Juan Martín
Reinko, Ivana Noelia
Boeris, Juan Martín
Cardozo, Dario Elbio
Baldo, Juan Diego
author_role author
author2 Ferro, Juan Martín
Reinko, Ivana Noelia
Boeris, Juan Martín
Cardozo, Dario Elbio
Baldo, Juan Diego
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv C-BANDING
CYTOGENETICS
HETEROCHROMATIN
HETEROMORPHISM
XY
topic C-BANDING
CYTOGENETICS
HETEROCHROMATIN
HETEROMORPHISM
XY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cytogenetic analyses were performed on specimens of Leptodactylus bufonius from different localities in Argentina. Mitotic chromosomes were studied with Giemsa and differential staining techniques (Ag-NOR, C-banding, and CMA3 /DAPI) and fl uorescence in situ hybridization with the 18S DNAr probe. All specimens showed karyotypes with 2n = 2x = 22 and FN = 44. Secondary constrictions were present in the long arm of chromosome pair 8, coincident with Ag-NOR and hybridization signals of the 18S DNAr probe. The C-banding technique evidenced an important amount of heterochromatin with a sex-linked pericentromeric band in the short arm of chromosome pair 4. This heterochromatic band was heteromorphic in males but present in both homologues of females, and it was CMA3 positive (DAPI negative) at fl uorescence staining. The occurrence of heteromorphic XY sex chromosomes in L. bufonius is the second known case in Leptodactylus and the fi fth within the speciose family Leptodactylidae.
Fil: Schneider, Rosio Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Fil: Ferro, Juan Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Fil: Reinko, Ivana Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Fil: Boeris, Juan Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Fil: Cardozo, Dario Elbio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
Fil: Baldo, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina
description Cytogenetic analyses were performed on specimens of Leptodactylus bufonius from different localities in Argentina. Mitotic chromosomes were studied with Giemsa and differential staining techniques (Ag-NOR, C-banding, and CMA3 /DAPI) and fl uorescence in situ hybridization with the 18S DNAr probe. All specimens showed karyotypes with 2n = 2x = 22 and FN = 44. Secondary constrictions were present in the long arm of chromosome pair 8, coincident with Ag-NOR and hybridization signals of the 18S DNAr probe. The C-banding technique evidenced an important amount of heterochromatin with a sex-linked pericentromeric band in the short arm of chromosome pair 4. This heterochromatic band was heteromorphic in males but present in both homologues of females, and it was CMA3 positive (DAPI negative) at fl uorescence staining. The occurrence of heteromorphic XY sex chromosomes in L. bufonius is the second known case in Leptodactylus and the fi fth within the speciose family Leptodactylidae.
publishDate 2021
dc.date.none.fl_str_mv 2021-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/157055
Schneider, Rosio Gabriela; Ferro, Juan Martín; Reinko, Ivana Noelia; Boeris, Juan Martín; Cardozo, Dario Elbio; et al.; Sex chromosomes in the Vizcacheras’ White-lipped frog, Leptodactylus bufonius (Anura, Leptodactylidae); Academia Brasilera de Ciencias; Anais da Academia Brasileira de Ciências; 93; 2; 6-2021; 1-12
0001-3765
1678-2690
CONICET Digital
CONICET
url http://hdl.handle.net/11336/157055
identifier_str_mv Schneider, Rosio Gabriela; Ferro, Juan Martín; Reinko, Ivana Noelia; Boeris, Juan Martín; Cardozo, Dario Elbio; et al.; Sex chromosomes in the Vizcacheras’ White-lipped frog, Leptodactylus bufonius (Anura, Leptodactylidae); Academia Brasilera de Ciencias; Anais da Academia Brasileira de Ciências; 93; 2; 6-2021; 1-12
0001-3765
1678-2690
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1590/0001-3765202120190426
info:eu-repo/semantics/altIdentifier/url/http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000301018&tlng=en
info:eu-repo/semantics/altIdentifier/url/https://www.ipni.org/p/3817-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academia Brasilera de Ciencias
publisher.none.fl_str_mv Academia Brasilera de Ciencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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