MART-1-and gp100-expressing and-non-expressing melanoma cells are equally proliferative in tumors and clonogenic in vitro

Autores
Aris, Mariana; Rodriguez Zubieta, Mariana; Colombo, Marina; Arriaga, Juan Martín; Bianchini, Michele; Alperovich, Myriam; Bravo, Alicia I.; Barrio, Maria Marcela; Mordoh, Jose
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
MART-1 and gp100 are prototypical melanoma antigen (Ag), but their clinical use as vaccines or as targets of cytotoxic lymphocytes achieved modest success. Possible explanations could be that as MART-1 and gp100 are melanocyte differentiation Ag, clonogenic Ag-non-expressing cells would be spared by immune effectors, or that clonogenic cells would be intrinsically resistant to cytotoxic lymphocytes. We therefore analyzed the proliferative status of MART-1/gp100-expressing and-non-expressing cells in biopsies, and the clonogenicity and sensitiveness to cytotoxic lymphocytes of the human cutaneous melanoma cell lines MEL-XY1 and MEL-XY3. Analysis of MART-1/gp100 and Ki-67 expression in 22 melanoma tumors revealed that MART-1/gp100-expressing and-non-expressing cells proliferated competitively. MART-1, gp100, tyrosinase, and CD271 expression were studied in MEL-XY1 and MEL-XY3 colonies. At 7 days, colonies displayed positive, negative, and mixed expression patterns. By 14 days, colonies of different sizes developed, showing cells with different clonogenic potential, and Ag were downregulated, suggesting Ag plasticity. Subcloning of MEL-XY1 colonies showed that Ag expression varied with time without interfering with clonogenicity. Finally, clonogenic, MART-1/gp100-expressing cells were lysed by specific CD8 lymphocytes. Thus, MART-1 and gp100 expression and plasticity would not interfere with proliferation or clonogenicity, and clonogenic cells may be lysed by cytotoxic lymphocytes.
Fil: Aris, Mariana. Instituto Alexander Fleming; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Rodriguez Zubieta, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Colombo, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Arriaga, Juan Martín. Instituto Alexander Fleming; Argentina
Fil: Bianchini, Michele. Instituto Alexander Fleming; Argentina
Fil: Alperovich, Myriam. Universidad de Buenos Aires; Argentina
Fil: Bravo, Alicia I.. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos "Eva Perón"; Argentina
Fil: Barrio, Maria Marcela. Instituto Alexander Fleming; Argentina
Fil: Mordoh, Jose. Instituto Alexander Fleming; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Materia
Melanoma
Mart-1
Gp100
Clonogenicity
Antigen Plasticity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/52562
Acceder
id CONICETDig_c200a210eb383b5609206e1610ca5702
oai_identifier_str oai:ri.conicet.gov.ar:11336/52562
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling MART-1-and gp100-expressing and-non-expressing melanoma cells are equally proliferative in tumors and clonogenic in vitroAris, MarianaRodriguez Zubieta, MarianaColombo, MarinaArriaga, Juan MartínBianchini, MicheleAlperovich, MyriamBravo, Alicia I.Barrio, Maria MarcelaMordoh, JoseMelanomaMart-1Gp100ClonogenicityAntigen Plasticityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1MART-1 and gp100 are prototypical melanoma antigen (Ag), but their clinical use as vaccines or as targets of cytotoxic lymphocytes achieved modest success. Possible explanations could be that as MART-1 and gp100 are melanocyte differentiation Ag, clonogenic Ag-non-expressing cells would be spared by immune effectors, or that clonogenic cells would be intrinsically resistant to cytotoxic lymphocytes. We therefore analyzed the proliferative status of MART-1/gp100-expressing and-non-expressing cells in biopsies, and the clonogenicity and sensitiveness to cytotoxic lymphocytes of the human cutaneous melanoma cell lines MEL-XY1 and MEL-XY3. Analysis of MART-1/gp100 and Ki-67 expression in 22 melanoma tumors revealed that MART-1/gp100-expressing and-non-expressing cells proliferated competitively. MART-1, gp100, tyrosinase, and CD271 expression were studied in MEL-XY1 and MEL-XY3 colonies. At 7 days, colonies displayed positive, negative, and mixed expression patterns. By 14 days, colonies of different sizes developed, showing cells with different clonogenic potential, and Ag were downregulated, suggesting Ag plasticity. Subcloning of MEL-XY1 colonies showed that Ag expression varied with time without interfering with clonogenicity. Finally, clonogenic, MART-1/gp100-expressing cells were lysed by specific CD8 lymphocytes. Thus, MART-1 and gp100 expression and plasticity would not interfere with proliferation or clonogenicity, and clonogenic cells may be lysed by cytotoxic lymphocytes.Fil: Aris, Mariana. Instituto Alexander Fleming; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Rodriguez Zubieta, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Colombo, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Arriaga, Juan Martín. Instituto Alexander Fleming; ArgentinaFil: Bianchini, Michele. Instituto Alexander Fleming; ArgentinaFil: Alperovich, Myriam. Universidad de Buenos Aires; ArgentinaFil: Bravo, Alicia I.. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos "Eva Perón"; ArgentinaFil: Barrio, Maria Marcela. Instituto Alexander Fleming; ArgentinaFil: Mordoh, Jose. Instituto Alexander Fleming; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaWilliams & Wilkins2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/52562Aris, Mariana; Rodriguez Zubieta, Mariana; Colombo, Marina; Arriaga, Juan Martín; Bianchini, Michele; et al.; MART-1-and gp100-expressing and-non-expressing melanoma cells are equally proliferative in tumors and clonogenic in vitro; Williams & Wilkins; Journal Of Investigative Dermatology; 132; 2; 2-2012; 365-3740022-202X1523-1747CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0022202X15355858info:eu-repo/semantics/altIdentifier/doi/10.1038/jid.2011.312info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:20:03Zoai:ri.conicet.gov.ar:11336/52562instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:20:03.328CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv MART-1-and gp100-expressing and-non-expressing melanoma cells are equally proliferative in tumors and clonogenic in vitro
title MART-1-and gp100-expressing and-non-expressing melanoma cells are equally proliferative in tumors and clonogenic in vitro
spellingShingle MART-1-and gp100-expressing and-non-expressing melanoma cells are equally proliferative in tumors and clonogenic in vitro
Aris, Mariana
Melanoma
Mart-1
Gp100
Clonogenicity
Antigen Plasticity
title_short MART-1-and gp100-expressing and-non-expressing melanoma cells are equally proliferative in tumors and clonogenic in vitro
title_full MART-1-and gp100-expressing and-non-expressing melanoma cells are equally proliferative in tumors and clonogenic in vitro
title_fullStr MART-1-and gp100-expressing and-non-expressing melanoma cells are equally proliferative in tumors and clonogenic in vitro
title_full_unstemmed MART-1-and gp100-expressing and-non-expressing melanoma cells are equally proliferative in tumors and clonogenic in vitro
title_sort MART-1-and gp100-expressing and-non-expressing melanoma cells are equally proliferative in tumors and clonogenic in vitro
dc.creator.none.fl_str_mv Aris, Mariana
Rodriguez Zubieta, Mariana
Colombo, Marina
Arriaga, Juan Martín
Bianchini, Michele
Alperovich, Myriam
Bravo, Alicia I.
Barrio, Maria Marcela
Mordoh, Jose
author Aris, Mariana
author_facet Aris, Mariana
Rodriguez Zubieta, Mariana
Colombo, Marina
Arriaga, Juan Martín
Bianchini, Michele
Alperovich, Myriam
Bravo, Alicia I.
Barrio, Maria Marcela
Mordoh, Jose
author_role author
author2 Rodriguez Zubieta, Mariana
Colombo, Marina
Arriaga, Juan Martín
Bianchini, Michele
Alperovich, Myriam
Bravo, Alicia I.
Barrio, Maria Marcela
Mordoh, Jose
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Melanoma
Mart-1
Gp100
Clonogenicity
Antigen Plasticity
topic Melanoma
Mart-1
Gp100
Clonogenicity
Antigen Plasticity
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv MART-1 and gp100 are prototypical melanoma antigen (Ag), but their clinical use as vaccines or as targets of cytotoxic lymphocytes achieved modest success. Possible explanations could be that as MART-1 and gp100 are melanocyte differentiation Ag, clonogenic Ag-non-expressing cells would be spared by immune effectors, or that clonogenic cells would be intrinsically resistant to cytotoxic lymphocytes. We therefore analyzed the proliferative status of MART-1/gp100-expressing and-non-expressing cells in biopsies, and the clonogenicity and sensitiveness to cytotoxic lymphocytes of the human cutaneous melanoma cell lines MEL-XY1 and MEL-XY3. Analysis of MART-1/gp100 and Ki-67 expression in 22 melanoma tumors revealed that MART-1/gp100-expressing and-non-expressing cells proliferated competitively. MART-1, gp100, tyrosinase, and CD271 expression were studied in MEL-XY1 and MEL-XY3 colonies. At 7 days, colonies displayed positive, negative, and mixed expression patterns. By 14 days, colonies of different sizes developed, showing cells with different clonogenic potential, and Ag were downregulated, suggesting Ag plasticity. Subcloning of MEL-XY1 colonies showed that Ag expression varied with time without interfering with clonogenicity. Finally, clonogenic, MART-1/gp100-expressing cells were lysed by specific CD8 lymphocytes. Thus, MART-1 and gp100 expression and plasticity would not interfere with proliferation or clonogenicity, and clonogenic cells may be lysed by cytotoxic lymphocytes.
Fil: Aris, Mariana. Instituto Alexander Fleming; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Rodriguez Zubieta, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Colombo, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Arriaga, Juan Martín. Instituto Alexander Fleming; Argentina
Fil: Bianchini, Michele. Instituto Alexander Fleming; Argentina
Fil: Alperovich, Myriam. Universidad de Buenos Aires; Argentina
Fil: Bravo, Alicia I.. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos "Eva Perón"; Argentina
Fil: Barrio, Maria Marcela. Instituto Alexander Fleming; Argentina
Fil: Mordoh, Jose. Instituto Alexander Fleming; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
description MART-1 and gp100 are prototypical melanoma antigen (Ag), but their clinical use as vaccines or as targets of cytotoxic lymphocytes achieved modest success. Possible explanations could be that as MART-1 and gp100 are melanocyte differentiation Ag, clonogenic Ag-non-expressing cells would be spared by immune effectors, or that clonogenic cells would be intrinsically resistant to cytotoxic lymphocytes. We therefore analyzed the proliferative status of MART-1/gp100-expressing and-non-expressing cells in biopsies, and the clonogenicity and sensitiveness to cytotoxic lymphocytes of the human cutaneous melanoma cell lines MEL-XY1 and MEL-XY3. Analysis of MART-1/gp100 and Ki-67 expression in 22 melanoma tumors revealed that MART-1/gp100-expressing and-non-expressing cells proliferated competitively. MART-1, gp100, tyrosinase, and CD271 expression were studied in MEL-XY1 and MEL-XY3 colonies. At 7 days, colonies displayed positive, negative, and mixed expression patterns. By 14 days, colonies of different sizes developed, showing cells with different clonogenic potential, and Ag were downregulated, suggesting Ag plasticity. Subcloning of MEL-XY1 colonies showed that Ag expression varied with time without interfering with clonogenicity. Finally, clonogenic, MART-1/gp100-expressing cells were lysed by specific CD8 lymphocytes. Thus, MART-1 and gp100 expression and plasticity would not interfere with proliferation or clonogenicity, and clonogenic cells may be lysed by cytotoxic lymphocytes.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/52562
Aris, Mariana; Rodriguez Zubieta, Mariana; Colombo, Marina; Arriaga, Juan Martín; Bianchini, Michele; et al.; MART-1-and gp100-expressing and-non-expressing melanoma cells are equally proliferative in tumors and clonogenic in vitro; Williams & Wilkins; Journal Of Investigative Dermatology; 132; 2; 2-2012; 365-374
0022-202X
1523-1747
CONICET Digital
CONICET
url http://hdl.handle.net/11336/52562
identifier_str_mv Aris, Mariana; Rodriguez Zubieta, Mariana; Colombo, Marina; Arriaga, Juan Martín; Bianchini, Michele; et al.; MART-1-and gp100-expressing and-non-expressing melanoma cells are equally proliferative in tumors and clonogenic in vitro; Williams & Wilkins; Journal Of Investigative Dermatology; 132; 2; 2-2012; 365-374
0022-202X
1523-1747
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0022202X15355858
info:eu-repo/semantics/altIdentifier/doi/10.1038/jid.2011.312
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Williams & Wilkins
publisher.none.fl_str_mv Williams & Wilkins
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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