Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer

Autores
Fragelli, Bruna; Almeida Rodolpho, Joice Margareth de; de Godoy, Krissia Frando; Líbero, Laura Ordonho; Granone, Luis Ignacio; Churio, Maria Sandra; Muniz Rennó, Ana Claudia; Freitas Anibal, Fernanda de; Longo, Elson; Assis, Marcelo
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bladder cancer presents a significant health challenge due to its high malignancy and rising incidence rates. Silver-based materials are well-known for their cytotoxic effects on various cell types. This study not only aimed to synthesize and characterize carbon-supported WO3:Ag heterostructures but also to evaluate their biological and physicochemical properties. The material was synthesized through the synergistic thermal decomposition of α-Ag2WO4 dispersed in chitosan, followed by WO3:Ag heterostructure formation on a carbon support, yielding samples with varying α-Ag2WO4 concentrations (SC, SC1, SC2, and SC4, for 0, 10, 20 and 40% of α-Ag2WO4 for chitosan). Characterization confirmed the successful formation of carbon-supported heterostructures with controlled ionic release and enhanced ROS generation. In vitro assays were conducted to assess the viability of non-tumor (3T3 fibroblasts) and tumor (bladder carcinoma MB49) cells using MTT salt and neutral red dye. Additional analyses included autophagy detection by correlating data from viability assays, nitric oxide and ROS quantification using the Griess reaction and fluorescent probes, and Caspase-3 activity measured with a fluorescent antibody. The results indicated that SC1 and SC2 samples were more effective against both cell types, with SC2 showing heightened effectiveness against the tumor lineage by inducing greater oxidative stress in MB49 cells compared to 3T3 fibroblasts. Additionally, the materials exhibited low ionic release (<0.01%), reducing potential adverse effects. Mechanistic analysis showed that the carbon support and synergistic interactions between WO₃ and Ag modulated ⦁OH radical production, even without light, enhancing the material's cytotoxic efficiency. These findings highlight the therapeutic potential of WO₃:Ag heterostructures as a safe and effective approach for treating aggressive cancers like bladder carcinoma, emphasizing the importance of further development in advanced biofunctional materials. This study also highlights the therapeutic potential of carbon-supported WO3:Ag heterostructures in bladder cancer treatment and underscores the importance of continued research in the development of novel anticancer strategies.
Fil: Fragelli, Bruna. Universidade Federal do São Carlos; Brasil
Fil: Almeida Rodolpho, Joice Margareth de. Universidade Federal do São Carlos; Brasil
Fil: de Godoy, Krissia Frando. Universidade Federal do São Carlos; Brasil
Fil: Líbero, Laura Ordonho. Universidade Federal do São Carlos; Brasil
Fil: Granone, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Físicas de Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Físicas de Mar del Plata; Argentina
Fil: Churio, Maria Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Físicas de Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Físicas de Mar del Plata; Argentina
Fil: Muniz Rennó, Ana Claudia. Universidade de Sao Paulo; Brasil
Fil: Freitas Anibal, Fernanda de. Universidade Federal do São Carlos; Brasil
Fil: Longo, Elson. Universidade Federal do São Carlos; Brasil
Fil: Assis, Marcelo. Universidade de Sao Paulo; Brasil
Materia
heterostructures
OH radicals
oxidative stress
EPR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/271329

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network_name_str CONICET Digital (CONICET)
spelling Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder CancerFragelli, BrunaAlmeida Rodolpho, Joice Margareth dede Godoy, Krissia FrandoLíbero, Laura OrdonhoGranone, Luis IgnacioChurio, Maria SandraMuniz Rennó, Ana ClaudiaFreitas Anibal, Fernanda deLongo, ElsonAssis, MarceloheterostructuresOH radicalsoxidative stressEPRhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Bladder cancer presents a significant health challenge due to its high malignancy and rising incidence rates. Silver-based materials are well-known for their cytotoxic effects on various cell types. This study not only aimed to synthesize and characterize carbon-supported WO3:Ag heterostructures but also to evaluate their biological and physicochemical properties. The material was synthesized through the synergistic thermal decomposition of α-Ag2WO4 dispersed in chitosan, followed by WO3:Ag heterostructure formation on a carbon support, yielding samples with varying α-Ag2WO4 concentrations (SC, SC1, SC2, and SC4, for 0, 10, 20 and 40% of α-Ag2WO4 for chitosan). Characterization confirmed the successful formation of carbon-supported heterostructures with controlled ionic release and enhanced ROS generation. In vitro assays were conducted to assess the viability of non-tumor (3T3 fibroblasts) and tumor (bladder carcinoma MB49) cells using MTT salt and neutral red dye. Additional analyses included autophagy detection by correlating data from viability assays, nitric oxide and ROS quantification using the Griess reaction and fluorescent probes, and Caspase-3 activity measured with a fluorescent antibody. The results indicated that SC1 and SC2 samples were more effective against both cell types, with SC2 showing heightened effectiveness against the tumor lineage by inducing greater oxidative stress in MB49 cells compared to 3T3 fibroblasts. Additionally, the materials exhibited low ionic release (<0.01%), reducing potential adverse effects. Mechanistic analysis showed that the carbon support and synergistic interactions between WO₃ and Ag modulated ⦁OH radical production, even without light, enhancing the material's cytotoxic efficiency. These findings highlight the therapeutic potential of WO₃:Ag heterostructures as a safe and effective approach for treating aggressive cancers like bladder carcinoma, emphasizing the importance of further development in advanced biofunctional materials. This study also highlights the therapeutic potential of carbon-supported WO3:Ag heterostructures in bladder cancer treatment and underscores the importance of continued research in the development of novel anticancer strategies.Fil: Fragelli, Bruna. Universidade Federal do São Carlos; BrasilFil: Almeida Rodolpho, Joice Margareth de. Universidade Federal do São Carlos; BrasilFil: de Godoy, Krissia Frando. Universidade Federal do São Carlos; BrasilFil: Líbero, Laura Ordonho. Universidade Federal do São Carlos; BrasilFil: Granone, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Físicas de Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Físicas de Mar del Plata; ArgentinaFil: Churio, Maria Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Físicas de Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Físicas de Mar del Plata; ArgentinaFil: Muniz Rennó, Ana Claudia. Universidade de Sao Paulo; BrasilFil: Freitas Anibal, Fernanda de. Universidade Federal do São Carlos; BrasilFil: Longo, Elson. Universidade Federal do São Carlos; BrasilFil: Assis, Marcelo. Universidade de Sao Paulo; BrasilInternational Journal of Pharmaceutical and Bio-Medical Science2024-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/271329Fragelli, Bruna; Almeida Rodolpho, Joice Margareth de; de Godoy, Krissia Frando; Líbero, Laura Ordonho; Granone, Luis Ignacio; et al.; Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer; International Journal of Pharmaceutical and Bio-Medical Science; International Journal of Pharmaceutical and Bio-Medical Science; 04; 12; 12-2024; 1-152767-827X2767-830XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ijpbms.com/index.php/ijpbms/article/view/643info:eu-repo/semantics/altIdentifier/doi/10.47191/ijpbms/v4-i12-07info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:38:47Zoai:ri.conicet.gov.ar:11336/271329instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:38:47.336CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer
title Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer
spellingShingle Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer
Fragelli, Bruna
heterostructures
OH radicals
oxidative stress
EPR
title_short Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer
title_full Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer
title_fullStr Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer
title_full_unstemmed Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer
title_sort Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer
dc.creator.none.fl_str_mv Fragelli, Bruna
Almeida Rodolpho, Joice Margareth de
de Godoy, Krissia Frando
Líbero, Laura Ordonho
Granone, Luis Ignacio
Churio, Maria Sandra
Muniz Rennó, Ana Claudia
Freitas Anibal, Fernanda de
Longo, Elson
Assis, Marcelo
author Fragelli, Bruna
author_facet Fragelli, Bruna
Almeida Rodolpho, Joice Margareth de
de Godoy, Krissia Frando
Líbero, Laura Ordonho
Granone, Luis Ignacio
Churio, Maria Sandra
Muniz Rennó, Ana Claudia
Freitas Anibal, Fernanda de
Longo, Elson
Assis, Marcelo
author_role author
author2 Almeida Rodolpho, Joice Margareth de
de Godoy, Krissia Frando
Líbero, Laura Ordonho
Granone, Luis Ignacio
Churio, Maria Sandra
Muniz Rennó, Ana Claudia
Freitas Anibal, Fernanda de
Longo, Elson
Assis, Marcelo
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv heterostructures
OH radicals
oxidative stress
EPR
topic heterostructures
OH radicals
oxidative stress
EPR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bladder cancer presents a significant health challenge due to its high malignancy and rising incidence rates. Silver-based materials are well-known for their cytotoxic effects on various cell types. This study not only aimed to synthesize and characterize carbon-supported WO3:Ag heterostructures but also to evaluate their biological and physicochemical properties. The material was synthesized through the synergistic thermal decomposition of α-Ag2WO4 dispersed in chitosan, followed by WO3:Ag heterostructure formation on a carbon support, yielding samples with varying α-Ag2WO4 concentrations (SC, SC1, SC2, and SC4, for 0, 10, 20 and 40% of α-Ag2WO4 for chitosan). Characterization confirmed the successful formation of carbon-supported heterostructures with controlled ionic release and enhanced ROS generation. In vitro assays were conducted to assess the viability of non-tumor (3T3 fibroblasts) and tumor (bladder carcinoma MB49) cells using MTT salt and neutral red dye. Additional analyses included autophagy detection by correlating data from viability assays, nitric oxide and ROS quantification using the Griess reaction and fluorescent probes, and Caspase-3 activity measured with a fluorescent antibody. The results indicated that SC1 and SC2 samples were more effective against both cell types, with SC2 showing heightened effectiveness against the tumor lineage by inducing greater oxidative stress in MB49 cells compared to 3T3 fibroblasts. Additionally, the materials exhibited low ionic release (<0.01%), reducing potential adverse effects. Mechanistic analysis showed that the carbon support and synergistic interactions between WO₃ and Ag modulated ⦁OH radical production, even without light, enhancing the material's cytotoxic efficiency. These findings highlight the therapeutic potential of WO₃:Ag heterostructures as a safe and effective approach for treating aggressive cancers like bladder carcinoma, emphasizing the importance of further development in advanced biofunctional materials. This study also highlights the therapeutic potential of carbon-supported WO3:Ag heterostructures in bladder cancer treatment and underscores the importance of continued research in the development of novel anticancer strategies.
Fil: Fragelli, Bruna. Universidade Federal do São Carlos; Brasil
Fil: Almeida Rodolpho, Joice Margareth de. Universidade Federal do São Carlos; Brasil
Fil: de Godoy, Krissia Frando. Universidade Federal do São Carlos; Brasil
Fil: Líbero, Laura Ordonho. Universidade Federal do São Carlos; Brasil
Fil: Granone, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Físicas de Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Físicas de Mar del Plata; Argentina
Fil: Churio, Maria Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Físicas de Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Físicas de Mar del Plata; Argentina
Fil: Muniz Rennó, Ana Claudia. Universidade de Sao Paulo; Brasil
Fil: Freitas Anibal, Fernanda de. Universidade Federal do São Carlos; Brasil
Fil: Longo, Elson. Universidade Federal do São Carlos; Brasil
Fil: Assis, Marcelo. Universidade de Sao Paulo; Brasil
description Bladder cancer presents a significant health challenge due to its high malignancy and rising incidence rates. Silver-based materials are well-known for their cytotoxic effects on various cell types. This study not only aimed to synthesize and characterize carbon-supported WO3:Ag heterostructures but also to evaluate their biological and physicochemical properties. The material was synthesized through the synergistic thermal decomposition of α-Ag2WO4 dispersed in chitosan, followed by WO3:Ag heterostructure formation on a carbon support, yielding samples with varying α-Ag2WO4 concentrations (SC, SC1, SC2, and SC4, for 0, 10, 20 and 40% of α-Ag2WO4 for chitosan). Characterization confirmed the successful formation of carbon-supported heterostructures with controlled ionic release and enhanced ROS generation. In vitro assays were conducted to assess the viability of non-tumor (3T3 fibroblasts) and tumor (bladder carcinoma MB49) cells using MTT salt and neutral red dye. Additional analyses included autophagy detection by correlating data from viability assays, nitric oxide and ROS quantification using the Griess reaction and fluorescent probes, and Caspase-3 activity measured with a fluorescent antibody. The results indicated that SC1 and SC2 samples were more effective against both cell types, with SC2 showing heightened effectiveness against the tumor lineage by inducing greater oxidative stress in MB49 cells compared to 3T3 fibroblasts. Additionally, the materials exhibited low ionic release (<0.01%), reducing potential adverse effects. Mechanistic analysis showed that the carbon support and synergistic interactions between WO₃ and Ag modulated ⦁OH radical production, even without light, enhancing the material's cytotoxic efficiency. These findings highlight the therapeutic potential of WO₃:Ag heterostructures as a safe and effective approach for treating aggressive cancers like bladder carcinoma, emphasizing the importance of further development in advanced biofunctional materials. This study also highlights the therapeutic potential of carbon-supported WO3:Ag heterostructures in bladder cancer treatment and underscores the importance of continued research in the development of novel anticancer strategies.
publishDate 2024
dc.date.none.fl_str_mv 2024-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/271329
Fragelli, Bruna; Almeida Rodolpho, Joice Margareth de; de Godoy, Krissia Frando; Líbero, Laura Ordonho; Granone, Luis Ignacio; et al.; Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer; International Journal of Pharmaceutical and Bio-Medical Science; International Journal of Pharmaceutical and Bio-Medical Science; 04; 12; 12-2024; 1-15
2767-827X
2767-830X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/271329
identifier_str_mv Fragelli, Bruna; Almeida Rodolpho, Joice Margareth de; de Godoy, Krissia Frando; Líbero, Laura Ordonho; Granone, Luis Ignacio; et al.; Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer; International Journal of Pharmaceutical and Bio-Medical Science; International Journal of Pharmaceutical and Bio-Medical Science; 04; 12; 12-2024; 1-15
2767-827X
2767-830X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ijpbms.com/index.php/ijpbms/article/view/643
info:eu-repo/semantics/altIdentifier/doi/10.47191/ijpbms/v4-i12-07
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Journal of Pharmaceutical and Bio-Medical Science
publisher.none.fl_str_mv International Journal of Pharmaceutical and Bio-Medical Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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