Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer
- Autores
- Fragelli, Bruna; Almeida Rodolpho, Joice Margareth de; de Godoy, Krissia Frando; Líbero, Laura Ordonho; Granone, Luis Ignacio; Churio, Maria Sandra; Muniz Rennó, Ana Claudia; Freitas Anibal, Fernanda de; Longo, Elson; Assis, Marcelo
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Bladder cancer presents a significant health challenge due to its high malignancy and rising incidence rates. Silver-based materials are well-known for their cytotoxic effects on various cell types. This study not only aimed to synthesize and characterize carbon-supported WO3:Ag heterostructures but also to evaluate their biological and physicochemical properties. The material was synthesized through the synergistic thermal decomposition of α-Ag2WO4 dispersed in chitosan, followed by WO3:Ag heterostructure formation on a carbon support, yielding samples with varying α-Ag2WO4 concentrations (SC, SC1, SC2, and SC4, for 0, 10, 20 and 40% of α-Ag2WO4 for chitosan). Characterization confirmed the successful formation of carbon-supported heterostructures with controlled ionic release and enhanced ROS generation. In vitro assays were conducted to assess the viability of non-tumor (3T3 fibroblasts) and tumor (bladder carcinoma MB49) cells using MTT salt and neutral red dye. Additional analyses included autophagy detection by correlating data from viability assays, nitric oxide and ROS quantification using the Griess reaction and fluorescent probes, and Caspase-3 activity measured with a fluorescent antibody. The results indicated that SC1 and SC2 samples were more effective against both cell types, with SC2 showing heightened effectiveness against the tumor lineage by inducing greater oxidative stress in MB49 cells compared to 3T3 fibroblasts. Additionally, the materials exhibited low ionic release (<0.01%), reducing potential adverse effects. Mechanistic analysis showed that the carbon support and synergistic interactions between WO₃ and Ag modulated ⦁OH radical production, even without light, enhancing the material's cytotoxic efficiency. These findings highlight the therapeutic potential of WO₃:Ag heterostructures as a safe and effective approach for treating aggressive cancers like bladder carcinoma, emphasizing the importance of further development in advanced biofunctional materials. This study also highlights the therapeutic potential of carbon-supported WO3:Ag heterostructures in bladder cancer treatment and underscores the importance of continued research in the development of novel anticancer strategies.
Fil: Fragelli, Bruna. Universidade Federal do São Carlos; Brasil
Fil: Almeida Rodolpho, Joice Margareth de. Universidade Federal do São Carlos; Brasil
Fil: de Godoy, Krissia Frando. Universidade Federal do São Carlos; Brasil
Fil: Líbero, Laura Ordonho. Universidade Federal do São Carlos; Brasil
Fil: Granone, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Físicas de Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Físicas de Mar del Plata; Argentina
Fil: Churio, Maria Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Físicas de Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Físicas de Mar del Plata; Argentina
Fil: Muniz Rennó, Ana Claudia. Universidade de Sao Paulo; Brasil
Fil: Freitas Anibal, Fernanda de. Universidade Federal do São Carlos; Brasil
Fil: Longo, Elson. Universidade Federal do São Carlos; Brasil
Fil: Assis, Marcelo. Universidade de Sao Paulo; Brasil - Materia
-
heterostructures
OH radicals
oxidative stress
EPR - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/271329
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Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder CancerFragelli, BrunaAlmeida Rodolpho, Joice Margareth dede Godoy, Krissia FrandoLíbero, Laura OrdonhoGranone, Luis IgnacioChurio, Maria SandraMuniz Rennó, Ana ClaudiaFreitas Anibal, Fernanda deLongo, ElsonAssis, MarceloheterostructuresOH radicalsoxidative stressEPRhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Bladder cancer presents a significant health challenge due to its high malignancy and rising incidence rates. Silver-based materials are well-known for their cytotoxic effects on various cell types. This study not only aimed to synthesize and characterize carbon-supported WO3:Ag heterostructures but also to evaluate their biological and physicochemical properties. The material was synthesized through the synergistic thermal decomposition of α-Ag2WO4 dispersed in chitosan, followed by WO3:Ag heterostructure formation on a carbon support, yielding samples with varying α-Ag2WO4 concentrations (SC, SC1, SC2, and SC4, for 0, 10, 20 and 40% of α-Ag2WO4 for chitosan). Characterization confirmed the successful formation of carbon-supported heterostructures with controlled ionic release and enhanced ROS generation. In vitro assays were conducted to assess the viability of non-tumor (3T3 fibroblasts) and tumor (bladder carcinoma MB49) cells using MTT salt and neutral red dye. Additional analyses included autophagy detection by correlating data from viability assays, nitric oxide and ROS quantification using the Griess reaction and fluorescent probes, and Caspase-3 activity measured with a fluorescent antibody. The results indicated that SC1 and SC2 samples were more effective against both cell types, with SC2 showing heightened effectiveness against the tumor lineage by inducing greater oxidative stress in MB49 cells compared to 3T3 fibroblasts. Additionally, the materials exhibited low ionic release (<0.01%), reducing potential adverse effects. Mechanistic analysis showed that the carbon support and synergistic interactions between WO₃ and Ag modulated ⦁OH radical production, even without light, enhancing the material's cytotoxic efficiency. These findings highlight the therapeutic potential of WO₃:Ag heterostructures as a safe and effective approach for treating aggressive cancers like bladder carcinoma, emphasizing the importance of further development in advanced biofunctional materials. This study also highlights the therapeutic potential of carbon-supported WO3:Ag heterostructures in bladder cancer treatment and underscores the importance of continued research in the development of novel anticancer strategies.Fil: Fragelli, Bruna. Universidade Federal do São Carlos; BrasilFil: Almeida Rodolpho, Joice Margareth de. Universidade Federal do São Carlos; BrasilFil: de Godoy, Krissia Frando. Universidade Federal do São Carlos; BrasilFil: Líbero, Laura Ordonho. Universidade Federal do São Carlos; BrasilFil: Granone, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Físicas de Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Físicas de Mar del Plata; ArgentinaFil: Churio, Maria Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Físicas de Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Físicas de Mar del Plata; ArgentinaFil: Muniz Rennó, Ana Claudia. Universidade de Sao Paulo; BrasilFil: Freitas Anibal, Fernanda de. Universidade Federal do São Carlos; BrasilFil: Longo, Elson. Universidade Federal do São Carlos; BrasilFil: Assis, Marcelo. Universidade de Sao Paulo; BrasilInternational Journal of Pharmaceutical and Bio-Medical Science2024-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/271329Fragelli, Bruna; Almeida Rodolpho, Joice Margareth de; de Godoy, Krissia Frando; Líbero, Laura Ordonho; Granone, Luis Ignacio; et al.; Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer; International Journal of Pharmaceutical and Bio-Medical Science; International Journal of Pharmaceutical and Bio-Medical Science; 04; 12; 12-2024; 1-152767-827X2767-830XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ijpbms.com/index.php/ijpbms/article/view/643info:eu-repo/semantics/altIdentifier/doi/10.47191/ijpbms/v4-i12-07info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:38:47Zoai:ri.conicet.gov.ar:11336/271329instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:38:47.336CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer |
title |
Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer |
spellingShingle |
Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer Fragelli, Bruna heterostructures OH radicals oxidative stress EPR |
title_short |
Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer |
title_full |
Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer |
title_fullStr |
Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer |
title_full_unstemmed |
Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer |
title_sort |
Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer |
dc.creator.none.fl_str_mv |
Fragelli, Bruna Almeida Rodolpho, Joice Margareth de de Godoy, Krissia Frando Líbero, Laura Ordonho Granone, Luis Ignacio Churio, Maria Sandra Muniz Rennó, Ana Claudia Freitas Anibal, Fernanda de Longo, Elson Assis, Marcelo |
author |
Fragelli, Bruna |
author_facet |
Fragelli, Bruna Almeida Rodolpho, Joice Margareth de de Godoy, Krissia Frando Líbero, Laura Ordonho Granone, Luis Ignacio Churio, Maria Sandra Muniz Rennó, Ana Claudia Freitas Anibal, Fernanda de Longo, Elson Assis, Marcelo |
author_role |
author |
author2 |
Almeida Rodolpho, Joice Margareth de de Godoy, Krissia Frando Líbero, Laura Ordonho Granone, Luis Ignacio Churio, Maria Sandra Muniz Rennó, Ana Claudia Freitas Anibal, Fernanda de Longo, Elson Assis, Marcelo |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
heterostructures OH radicals oxidative stress EPR |
topic |
heterostructures OH radicals oxidative stress EPR |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Bladder cancer presents a significant health challenge due to its high malignancy and rising incidence rates. Silver-based materials are well-known for their cytotoxic effects on various cell types. This study not only aimed to synthesize and characterize carbon-supported WO3:Ag heterostructures but also to evaluate their biological and physicochemical properties. The material was synthesized through the synergistic thermal decomposition of α-Ag2WO4 dispersed in chitosan, followed by WO3:Ag heterostructure formation on a carbon support, yielding samples with varying α-Ag2WO4 concentrations (SC, SC1, SC2, and SC4, for 0, 10, 20 and 40% of α-Ag2WO4 for chitosan). Characterization confirmed the successful formation of carbon-supported heterostructures with controlled ionic release and enhanced ROS generation. In vitro assays were conducted to assess the viability of non-tumor (3T3 fibroblasts) and tumor (bladder carcinoma MB49) cells using MTT salt and neutral red dye. Additional analyses included autophagy detection by correlating data from viability assays, nitric oxide and ROS quantification using the Griess reaction and fluorescent probes, and Caspase-3 activity measured with a fluorescent antibody. The results indicated that SC1 and SC2 samples were more effective against both cell types, with SC2 showing heightened effectiveness against the tumor lineage by inducing greater oxidative stress in MB49 cells compared to 3T3 fibroblasts. Additionally, the materials exhibited low ionic release (<0.01%), reducing potential adverse effects. Mechanistic analysis showed that the carbon support and synergistic interactions between WO₃ and Ag modulated ⦁OH radical production, even without light, enhancing the material's cytotoxic efficiency. These findings highlight the therapeutic potential of WO₃:Ag heterostructures as a safe and effective approach for treating aggressive cancers like bladder carcinoma, emphasizing the importance of further development in advanced biofunctional materials. This study also highlights the therapeutic potential of carbon-supported WO3:Ag heterostructures in bladder cancer treatment and underscores the importance of continued research in the development of novel anticancer strategies. Fil: Fragelli, Bruna. Universidade Federal do São Carlos; Brasil Fil: Almeida Rodolpho, Joice Margareth de. Universidade Federal do São Carlos; Brasil Fil: de Godoy, Krissia Frando. Universidade Federal do São Carlos; Brasil Fil: Líbero, Laura Ordonho. Universidade Federal do São Carlos; Brasil Fil: Granone, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Físicas de Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Físicas de Mar del Plata; Argentina Fil: Churio, Maria Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Físicas de Mar del Plata. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Físicas de Mar del Plata; Argentina Fil: Muniz Rennó, Ana Claudia. Universidade de Sao Paulo; Brasil Fil: Freitas Anibal, Fernanda de. Universidade Federal do São Carlos; Brasil Fil: Longo, Elson. Universidade Federal do São Carlos; Brasil Fil: Assis, Marcelo. Universidade de Sao Paulo; Brasil |
description |
Bladder cancer presents a significant health challenge due to its high malignancy and rising incidence rates. Silver-based materials are well-known for their cytotoxic effects on various cell types. This study not only aimed to synthesize and characterize carbon-supported WO3:Ag heterostructures but also to evaluate their biological and physicochemical properties. The material was synthesized through the synergistic thermal decomposition of α-Ag2WO4 dispersed in chitosan, followed by WO3:Ag heterostructure formation on a carbon support, yielding samples with varying α-Ag2WO4 concentrations (SC, SC1, SC2, and SC4, for 0, 10, 20 and 40% of α-Ag2WO4 for chitosan). Characterization confirmed the successful formation of carbon-supported heterostructures with controlled ionic release and enhanced ROS generation. In vitro assays were conducted to assess the viability of non-tumor (3T3 fibroblasts) and tumor (bladder carcinoma MB49) cells using MTT salt and neutral red dye. Additional analyses included autophagy detection by correlating data from viability assays, nitric oxide and ROS quantification using the Griess reaction and fluorescent probes, and Caspase-3 activity measured with a fluorescent antibody. The results indicated that SC1 and SC2 samples were more effective against both cell types, with SC2 showing heightened effectiveness against the tumor lineage by inducing greater oxidative stress in MB49 cells compared to 3T3 fibroblasts. Additionally, the materials exhibited low ionic release (<0.01%), reducing potential adverse effects. Mechanistic analysis showed that the carbon support and synergistic interactions between WO₃ and Ag modulated ⦁OH radical production, even without light, enhancing the material's cytotoxic efficiency. These findings highlight the therapeutic potential of WO₃:Ag heterostructures as a safe and effective approach for treating aggressive cancers like bladder carcinoma, emphasizing the importance of further development in advanced biofunctional materials. This study also highlights the therapeutic potential of carbon-supported WO3:Ag heterostructures in bladder cancer treatment and underscores the importance of continued research in the development of novel anticancer strategies. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/271329 Fragelli, Bruna; Almeida Rodolpho, Joice Margareth de; de Godoy, Krissia Frando; Líbero, Laura Ordonho; Granone, Luis Ignacio; et al.; Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer; International Journal of Pharmaceutical and Bio-Medical Science; International Journal of Pharmaceutical and Bio-Medical Science; 04; 12; 12-2024; 1-15 2767-827X 2767-830X CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/271329 |
identifier_str_mv |
Fragelli, Bruna; Almeida Rodolpho, Joice Margareth de; de Godoy, Krissia Frando; Líbero, Laura Ordonho; Granone, Luis Ignacio; et al.; Investigation of ROS-Driven Cytotoxic Mechanisms in WO3: Ag Heterostructures Supported on Carbon against Bladder Cancer; International Journal of Pharmaceutical and Bio-Medical Science; International Journal of Pharmaceutical and Bio-Medical Science; 04; 12; 12-2024; 1-15 2767-827X 2767-830X CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://ijpbms.com/index.php/ijpbms/article/view/643 info:eu-repo/semantics/altIdentifier/doi/10.47191/ijpbms/v4-i12-07 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
International Journal of Pharmaceutical and Bio-Medical Science |
publisher.none.fl_str_mv |
International Journal of Pharmaceutical and Bio-Medical Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614411653742592 |
score |
13.070432 |