B-casein hydrolysate generated by the cell envelope-associated proteinase of Lactobacillus delbrueckii subsp. lactis CRL 581 protects against trinitrobenzene sulfonic acid-induced...
- Autores
- Espeche Turbay, Maria Beatriz; de Moreno, Maria Alejandra; Perdigon, Gabriela del Valle; Savoy, Graciela; Hebert, Elvira Maria
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Lactobacillus delbrueckii subsp. lactis CRL 581, a thermophilic lactic acid bacterium used as a starter culture for the manufacture of several fermented dairy products, possess an efficient proteolytic system which is able to release a series of potentially bioactive peptides (i.e. antihypertensive and phosphopeptides) from alpha- and beta-caseins. Considering the potential beneficial health effects of the peptides released by L. delbrueckii subsp. lactis CRL 581 from milk proteins, the aim of this work was to analyze the antimutagenic and anti-inflammatory properties of the casein hydrolysates generated by the cell envelope-associated proteinase of this bacterium. The ability of alpha- and beta-casein hydrolysates to suppress the mutagenesis of a direct-acting mutagen 4-nitroquinoline-N-oxide on Salmonella typhimurium TA 98 and TA 100 increased concomitantly with the time of casein hydrolysis. The anti-inflammatory effect of the beta-casein hydrolysate was evaluated using a trinitrobenzene sulfonic acid (TNBS) induced Crohn’s disease murine model. The hydrolysate was administered to mice 10 days before the intrarectal inoculation of TNBS. The mice that received B-casein hydrolysate previously to TNBS showed decreased mortality rates, faster recovery of initial body weight loss, lesser microbial translocation to the liver, decreased B-glucuronidase and myeloperoxidase activities in the gut and reduced colonic macroscopic and microscopic damages compared to the animals that did not receive this hydrolysate. In addition, B-casein hydrolysate exerted a beneficial effect on acute intestinal inflammation by increased interleukin 10 and decreased IFN-gamma production in the gut. Our findings demonstrated the health promoting attributes of the milk products fermented by L. delbrueckii subsp. lactis CRL 581 and open up new opportunities for developing novel functional foods.
Fil: Espeche Turbay, Maria Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina - Materia
-
Bioactive Peptide
Lactic Acid Bacteria
Casein Hydrolysate
Proteinase - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/21786
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B-casein hydrolysate generated by the cell envelope-associated proteinase of Lactobacillus delbrueckii subsp. lactis CRL 581 protects against trinitrobenzene sulfonic acid-induced colitis in miceEspeche Turbay, Maria Beatrizde Moreno, Maria AlejandraPerdigon, Gabriela del ValleSavoy, GracielaHebert, Elvira MariaBioactive PeptideLactic Acid BacteriaCasein HydrolysateProteinasehttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Lactobacillus delbrueckii subsp. lactis CRL 581, a thermophilic lactic acid bacterium used as a starter culture for the manufacture of several fermented dairy products, possess an efficient proteolytic system which is able to release a series of potentially bioactive peptides (i.e. antihypertensive and phosphopeptides) from alpha- and beta-caseins. Considering the potential beneficial health effects of the peptides released by L. delbrueckii subsp. lactis CRL 581 from milk proteins, the aim of this work was to analyze the antimutagenic and anti-inflammatory properties of the casein hydrolysates generated by the cell envelope-associated proteinase of this bacterium. The ability of alpha- and beta-casein hydrolysates to suppress the mutagenesis of a direct-acting mutagen 4-nitroquinoline-N-oxide on Salmonella typhimurium TA 98 and TA 100 increased concomitantly with the time of casein hydrolysis. The anti-inflammatory effect of the beta-casein hydrolysate was evaluated using a trinitrobenzene sulfonic acid (TNBS) induced Crohn’s disease murine model. The hydrolysate was administered to mice 10 days before the intrarectal inoculation of TNBS. The mice that received B-casein hydrolysate previously to TNBS showed decreased mortality rates, faster recovery of initial body weight loss, lesser microbial translocation to the liver, decreased B-glucuronidase and myeloperoxidase activities in the gut and reduced colonic macroscopic and microscopic damages compared to the animals that did not receive this hydrolysate. In addition, B-casein hydrolysate exerted a beneficial effect on acute intestinal inflammation by increased interleukin 10 and decreased IFN-gamma production in the gut. Our findings demonstrated the health promoting attributes of the milk products fermented by L. delbrueckii subsp. lactis CRL 581 and open up new opportunities for developing novel functional foods.Fil: Espeche Turbay, Maria Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaElsevier2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/21786Espeche Turbay, Maria Beatriz; de Moreno, Maria Alejandra; Perdigon, Gabriela del Valle; Savoy, Graciela; Hebert, Elvira Maria; B-casein hydrolysate generated by the cell envelope-associated proteinase of Lactobacillus delbrueckii subsp. lactis CRL 581 protects against trinitrobenzene sulfonic acid-induced colitis in mice; Elsevier; Journal of Dairy Science; 95; 3; 2-2012; 1108-11180022-03021525-3198CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3168/jds.2011-4735info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022030212000690info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:42Zoai:ri.conicet.gov.ar:11336/21786instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:43.078CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
B-casein hydrolysate generated by the cell envelope-associated proteinase of Lactobacillus delbrueckii subsp. lactis CRL 581 protects against trinitrobenzene sulfonic acid-induced colitis in mice |
title |
B-casein hydrolysate generated by the cell envelope-associated proteinase of Lactobacillus delbrueckii subsp. lactis CRL 581 protects against trinitrobenzene sulfonic acid-induced colitis in mice |
spellingShingle |
B-casein hydrolysate generated by the cell envelope-associated proteinase of Lactobacillus delbrueckii subsp. lactis CRL 581 protects against trinitrobenzene sulfonic acid-induced colitis in mice Espeche Turbay, Maria Beatriz Bioactive Peptide Lactic Acid Bacteria Casein Hydrolysate Proteinase |
title_short |
B-casein hydrolysate generated by the cell envelope-associated proteinase of Lactobacillus delbrueckii subsp. lactis CRL 581 protects against trinitrobenzene sulfonic acid-induced colitis in mice |
title_full |
B-casein hydrolysate generated by the cell envelope-associated proteinase of Lactobacillus delbrueckii subsp. lactis CRL 581 protects against trinitrobenzene sulfonic acid-induced colitis in mice |
title_fullStr |
B-casein hydrolysate generated by the cell envelope-associated proteinase of Lactobacillus delbrueckii subsp. lactis CRL 581 protects against trinitrobenzene sulfonic acid-induced colitis in mice |
title_full_unstemmed |
B-casein hydrolysate generated by the cell envelope-associated proteinase of Lactobacillus delbrueckii subsp. lactis CRL 581 protects against trinitrobenzene sulfonic acid-induced colitis in mice |
title_sort |
B-casein hydrolysate generated by the cell envelope-associated proteinase of Lactobacillus delbrueckii subsp. lactis CRL 581 protects against trinitrobenzene sulfonic acid-induced colitis in mice |
dc.creator.none.fl_str_mv |
Espeche Turbay, Maria Beatriz de Moreno, Maria Alejandra Perdigon, Gabriela del Valle Savoy, Graciela Hebert, Elvira Maria |
author |
Espeche Turbay, Maria Beatriz |
author_facet |
Espeche Turbay, Maria Beatriz de Moreno, Maria Alejandra Perdigon, Gabriela del Valle Savoy, Graciela Hebert, Elvira Maria |
author_role |
author |
author2 |
de Moreno, Maria Alejandra Perdigon, Gabriela del Valle Savoy, Graciela Hebert, Elvira Maria |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Bioactive Peptide Lactic Acid Bacteria Casein Hydrolysate Proteinase |
topic |
Bioactive Peptide Lactic Acid Bacteria Casein Hydrolysate Proteinase |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Lactobacillus delbrueckii subsp. lactis CRL 581, a thermophilic lactic acid bacterium used as a starter culture for the manufacture of several fermented dairy products, possess an efficient proteolytic system which is able to release a series of potentially bioactive peptides (i.e. antihypertensive and phosphopeptides) from alpha- and beta-caseins. Considering the potential beneficial health effects of the peptides released by L. delbrueckii subsp. lactis CRL 581 from milk proteins, the aim of this work was to analyze the antimutagenic and anti-inflammatory properties of the casein hydrolysates generated by the cell envelope-associated proteinase of this bacterium. The ability of alpha- and beta-casein hydrolysates to suppress the mutagenesis of a direct-acting mutagen 4-nitroquinoline-N-oxide on Salmonella typhimurium TA 98 and TA 100 increased concomitantly with the time of casein hydrolysis. The anti-inflammatory effect of the beta-casein hydrolysate was evaluated using a trinitrobenzene sulfonic acid (TNBS) induced Crohn’s disease murine model. The hydrolysate was administered to mice 10 days before the intrarectal inoculation of TNBS. The mice that received B-casein hydrolysate previously to TNBS showed decreased mortality rates, faster recovery of initial body weight loss, lesser microbial translocation to the liver, decreased B-glucuronidase and myeloperoxidase activities in the gut and reduced colonic macroscopic and microscopic damages compared to the animals that did not receive this hydrolysate. In addition, B-casein hydrolysate exerted a beneficial effect on acute intestinal inflammation by increased interleukin 10 and decreased IFN-gamma production in the gut. Our findings demonstrated the health promoting attributes of the milk products fermented by L. delbrueckii subsp. lactis CRL 581 and open up new opportunities for developing novel functional foods. Fil: Espeche Turbay, Maria Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: Savoy, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina Fil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina |
description |
Lactobacillus delbrueckii subsp. lactis CRL 581, a thermophilic lactic acid bacterium used as a starter culture for the manufacture of several fermented dairy products, possess an efficient proteolytic system which is able to release a series of potentially bioactive peptides (i.e. antihypertensive and phosphopeptides) from alpha- and beta-caseins. Considering the potential beneficial health effects of the peptides released by L. delbrueckii subsp. lactis CRL 581 from milk proteins, the aim of this work was to analyze the antimutagenic and anti-inflammatory properties of the casein hydrolysates generated by the cell envelope-associated proteinase of this bacterium. The ability of alpha- and beta-casein hydrolysates to suppress the mutagenesis of a direct-acting mutagen 4-nitroquinoline-N-oxide on Salmonella typhimurium TA 98 and TA 100 increased concomitantly with the time of casein hydrolysis. The anti-inflammatory effect of the beta-casein hydrolysate was evaluated using a trinitrobenzene sulfonic acid (TNBS) induced Crohn’s disease murine model. The hydrolysate was administered to mice 10 days before the intrarectal inoculation of TNBS. The mice that received B-casein hydrolysate previously to TNBS showed decreased mortality rates, faster recovery of initial body weight loss, lesser microbial translocation to the liver, decreased B-glucuronidase and myeloperoxidase activities in the gut and reduced colonic macroscopic and microscopic damages compared to the animals that did not receive this hydrolysate. In addition, B-casein hydrolysate exerted a beneficial effect on acute intestinal inflammation by increased interleukin 10 and decreased IFN-gamma production in the gut. Our findings demonstrated the health promoting attributes of the milk products fermented by L. delbrueckii subsp. lactis CRL 581 and open up new opportunities for developing novel functional foods. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/21786 Espeche Turbay, Maria Beatriz; de Moreno, Maria Alejandra; Perdigon, Gabriela del Valle; Savoy, Graciela; Hebert, Elvira Maria; B-casein hydrolysate generated by the cell envelope-associated proteinase of Lactobacillus delbrueckii subsp. lactis CRL 581 protects against trinitrobenzene sulfonic acid-induced colitis in mice; Elsevier; Journal of Dairy Science; 95; 3; 2-2012; 1108-1118 0022-0302 1525-3198 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/21786 |
identifier_str_mv |
Espeche Turbay, Maria Beatriz; de Moreno, Maria Alejandra; Perdigon, Gabriela del Valle; Savoy, Graciela; Hebert, Elvira Maria; B-casein hydrolysate generated by the cell envelope-associated proteinase of Lactobacillus delbrueckii subsp. lactis CRL 581 protects against trinitrobenzene sulfonic acid-induced colitis in mice; Elsevier; Journal of Dairy Science; 95; 3; 2-2012; 1108-1118 0022-0302 1525-3198 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.3168/jds.2011-4735 info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022030212000690 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844614521297043456 |
score |
13.070432 |