Transcriptional Responses of Herbaspirillum seropedicae to Environmental Phosphate Concentration

Autores
Grillo Puertas, Mariana; Villegas, Josefina Maria; Pankievicz, Vânia C. S.; Tadra-Sfeir, Michelle Z.; Teles Mota, Francisco J.; Hebert, Elvira Maria; Brusamarello Santos, Liziane; Pedraza, Raúl Osvaldo; Pedrosa, Fabio O.; Rapisarda, Viviana Andrea; Souza, Emanuel M.
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Herbaspirillum seropedicae is a nitrogen-fixing endophytic bacterium associated with important cereal crops, which promotes plant growth, increasing their productivity. The understanding of the physiological responses of this bacterium to different concentrations of prevailing nutrients as phosphate (Pi) is scarce. In some bacteria, culture media Pi concentration modulates the levels of intracellular polyphosphate (polyP), modifying their cellular fitness. Here, global changes of H. seropedicae SmR1 were evaluated in response to environmental Pi concentrations, based on differential intracellular polyP levels. Cells grown in high-Pi medium (50 mM) maintained high polyP levels in stationary phase, while those grown in sufficient Pi medium (5 mM) degraded it. Through a RNA-seq approach, comparison of transcriptional profiles of H. seropedicae cultures revealed that 670 genes were differentially expressed between both Pi growth conditions, with 57% repressed and 43% induced in the high Pi condition. Molecular and physiological analyses revealed that aspects related to Pi metabolism, biosynthesis of flagella and chemotaxis, energy production, and polyhydroxybutyrate metabolism were induced in the high-Pi condition, while those involved in adhesion and stress response were repressed. The present study demonstrated that variations in environmental Pi concentration affect H. seropedicae traits related to survival and other important physiological characteristics. Since environmental conditions can influence the effectiveness of the plant growth-promoting bacteria, enhancement of bacterial robustness to withstand different stressful situations is an interesting challenge. The obtained data could serve not only to understand the bacterial behavior in respect to changes in rhizospheric Pi gradients but also as a base to design strategies to improve different bacterial features focusing on biotechnological and/or agricultural purposes.
Fil: Grillo Puertas, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Villegas, Josefina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pankievicz, Vânia C. S.. Universidade Federal do Paraná; Brasil
Fil: Tadra-Sfeir, Michelle Z.. Universidade Federal do Paraná; Brasil
Fil: Teles Mota, Francisco J.. Universidade Federal do Paraná; Brasil
Fil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Brusamarello Santos, Liziane. Universidade Federal do Paraná; Brasil
Fil: Pedraza, Raúl Osvaldo. Universidad Nacional de Tucumán; Argentina
Fil: Pedrosa, Fabio O.. Universidade Federal do Paraná; Brasil
Fil: Rapisarda, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Souza, Emanuel M.. Universidade Federal do Paraná; Brasil
Materia
ADHESION
HERBASPIRILLUM SEROPEDICAE
PHOSPHATE
PLANT-MICROBE INTERACTION
POLYPHOSPHATE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184370
Acceder
id CONICETDig_c170388ffb253b7c71f1434c6668e355
oai_identifier_str oai:ri.conicet.gov.ar:11336/184370
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transcriptional Responses of Herbaspirillum seropedicae to Environmental Phosphate ConcentrationGrillo Puertas, MarianaVillegas, Josefina MariaPankievicz, Vânia C. S.Tadra-Sfeir, Michelle Z.Teles Mota, Francisco J.Hebert, Elvira MariaBrusamarello Santos, LizianePedraza, Raúl OsvaldoPedrosa, Fabio O.Rapisarda, Viviana AndreaSouza, Emanuel M.ADHESIONHERBASPIRILLUM SEROPEDICAEPHOSPHATEPLANT-MICROBE INTERACTIONPOLYPHOSPHATEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Herbaspirillum seropedicae is a nitrogen-fixing endophytic bacterium associated with important cereal crops, which promotes plant growth, increasing their productivity. The understanding of the physiological responses of this bacterium to different concentrations of prevailing nutrients as phosphate (Pi) is scarce. In some bacteria, culture media Pi concentration modulates the levels of intracellular polyphosphate (polyP), modifying their cellular fitness. Here, global changes of H. seropedicae SmR1 were evaluated in response to environmental Pi concentrations, based on differential intracellular polyP levels. Cells grown in high-Pi medium (50 mM) maintained high polyP levels in stationary phase, while those grown in sufficient Pi medium (5 mM) degraded it. Through a RNA-seq approach, comparison of transcriptional profiles of H. seropedicae cultures revealed that 670 genes were differentially expressed between both Pi growth conditions, with 57% repressed and 43% induced in the high Pi condition. Molecular and physiological analyses revealed that aspects related to Pi metabolism, biosynthesis of flagella and chemotaxis, energy production, and polyhydroxybutyrate metabolism were induced in the high-Pi condition, while those involved in adhesion and stress response were repressed. The present study demonstrated that variations in environmental Pi concentration affect H. seropedicae traits related to survival and other important physiological characteristics. Since environmental conditions can influence the effectiveness of the plant growth-promoting bacteria, enhancement of bacterial robustness to withstand different stressful situations is an interesting challenge. The obtained data could serve not only to understand the bacterial behavior in respect to changes in rhizospheric Pi gradients but also as a base to design strategies to improve different bacterial features focusing on biotechnological and/or agricultural purposes.Fil: Grillo Puertas, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Villegas, Josefina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Pankievicz, Vânia C. S.. Universidade Federal do Paraná; BrasilFil: Tadra-Sfeir, Michelle Z.. Universidade Federal do Paraná; BrasilFil: Teles Mota, Francisco J.. Universidade Federal do Paraná; BrasilFil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Brusamarello Santos, Liziane. Universidade Federal do Paraná; BrasilFil: Pedraza, Raúl Osvaldo. Universidad Nacional de Tucumán; ArgentinaFil: Pedrosa, Fabio O.. Universidade Federal do Paraná; BrasilFil: Rapisarda, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Souza, Emanuel M.. Universidade Federal do Paraná; BrasilFrontiers Media2021-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184370Grillo Puertas, Mariana; Villegas, Josefina Maria; Pankievicz, Vânia C. S.; Tadra-Sfeir, Michelle Z.; Teles Mota, Francisco J.; et al.; Transcriptional Responses of Herbaspirillum seropedicae to Environmental Phosphate Concentration; Frontiers Media; Frontiers in Microbiology; 12; 6-2021; 1-151664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2021.666277info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:49:42Zoai:ri.conicet.gov.ar:11336/184370instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:49:42.97CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transcriptional Responses of Herbaspirillum seropedicae to Environmental Phosphate Concentration
title Transcriptional Responses of Herbaspirillum seropedicae to Environmental Phosphate Concentration
spellingShingle Transcriptional Responses of Herbaspirillum seropedicae to Environmental Phosphate Concentration
Grillo Puertas, Mariana
ADHESION
HERBASPIRILLUM SEROPEDICAE
PHOSPHATE
PLANT-MICROBE INTERACTION
POLYPHOSPHATE
title_short Transcriptional Responses of Herbaspirillum seropedicae to Environmental Phosphate Concentration
title_full Transcriptional Responses of Herbaspirillum seropedicae to Environmental Phosphate Concentration
title_fullStr Transcriptional Responses of Herbaspirillum seropedicae to Environmental Phosphate Concentration
title_full_unstemmed Transcriptional Responses of Herbaspirillum seropedicae to Environmental Phosphate Concentration
title_sort Transcriptional Responses of Herbaspirillum seropedicae to Environmental Phosphate Concentration
dc.creator.none.fl_str_mv Grillo Puertas, Mariana
Villegas, Josefina Maria
Pankievicz, Vânia C. S.
Tadra-Sfeir, Michelle Z.
Teles Mota, Francisco J.
Hebert, Elvira Maria
Brusamarello Santos, Liziane
Pedraza, Raúl Osvaldo
Pedrosa, Fabio O.
Rapisarda, Viviana Andrea
Souza, Emanuel M.
author Grillo Puertas, Mariana
author_facet Grillo Puertas, Mariana
Villegas, Josefina Maria
Pankievicz, Vânia C. S.
Tadra-Sfeir, Michelle Z.
Teles Mota, Francisco J.
Hebert, Elvira Maria
Brusamarello Santos, Liziane
Pedraza, Raúl Osvaldo
Pedrosa, Fabio O.
Rapisarda, Viviana Andrea
Souza, Emanuel M.
author_role author
author2 Villegas, Josefina Maria
Pankievicz, Vânia C. S.
Tadra-Sfeir, Michelle Z.
Teles Mota, Francisco J.
Hebert, Elvira Maria
Brusamarello Santos, Liziane
Pedraza, Raúl Osvaldo
Pedrosa, Fabio O.
Rapisarda, Viviana Andrea
Souza, Emanuel M.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ADHESION
HERBASPIRILLUM SEROPEDICAE
PHOSPHATE
PLANT-MICROBE INTERACTION
POLYPHOSPHATE
topic ADHESION
HERBASPIRILLUM SEROPEDICAE
PHOSPHATE
PLANT-MICROBE INTERACTION
POLYPHOSPHATE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Herbaspirillum seropedicae is a nitrogen-fixing endophytic bacterium associated with important cereal crops, which promotes plant growth, increasing their productivity. The understanding of the physiological responses of this bacterium to different concentrations of prevailing nutrients as phosphate (Pi) is scarce. In some bacteria, culture media Pi concentration modulates the levels of intracellular polyphosphate (polyP), modifying their cellular fitness. Here, global changes of H. seropedicae SmR1 were evaluated in response to environmental Pi concentrations, based on differential intracellular polyP levels. Cells grown in high-Pi medium (50 mM) maintained high polyP levels in stationary phase, while those grown in sufficient Pi medium (5 mM) degraded it. Through a RNA-seq approach, comparison of transcriptional profiles of H. seropedicae cultures revealed that 670 genes were differentially expressed between both Pi growth conditions, with 57% repressed and 43% induced in the high Pi condition. Molecular and physiological analyses revealed that aspects related to Pi metabolism, biosynthesis of flagella and chemotaxis, energy production, and polyhydroxybutyrate metabolism were induced in the high-Pi condition, while those involved in adhesion and stress response were repressed. The present study demonstrated that variations in environmental Pi concentration affect H. seropedicae traits related to survival and other important physiological characteristics. Since environmental conditions can influence the effectiveness of the plant growth-promoting bacteria, enhancement of bacterial robustness to withstand different stressful situations is an interesting challenge. The obtained data could serve not only to understand the bacterial behavior in respect to changes in rhizospheric Pi gradients but also as a base to design strategies to improve different bacterial features focusing on biotechnological and/or agricultural purposes.
Fil: Grillo Puertas, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Villegas, Josefina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pankievicz, Vânia C. S.. Universidade Federal do Paraná; Brasil
Fil: Tadra-Sfeir, Michelle Z.. Universidade Federal do Paraná; Brasil
Fil: Teles Mota, Francisco J.. Universidade Federal do Paraná; Brasil
Fil: Hebert, Elvira Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Brusamarello Santos, Liziane. Universidade Federal do Paraná; Brasil
Fil: Pedraza, Raúl Osvaldo. Universidad Nacional de Tucumán; Argentina
Fil: Pedrosa, Fabio O.. Universidade Federal do Paraná; Brasil
Fil: Rapisarda, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Souza, Emanuel M.. Universidade Federal do Paraná; Brasil
description Herbaspirillum seropedicae is a nitrogen-fixing endophytic bacterium associated with important cereal crops, which promotes plant growth, increasing their productivity. The understanding of the physiological responses of this bacterium to different concentrations of prevailing nutrients as phosphate (Pi) is scarce. In some bacteria, culture media Pi concentration modulates the levels of intracellular polyphosphate (polyP), modifying their cellular fitness. Here, global changes of H. seropedicae SmR1 were evaluated in response to environmental Pi concentrations, based on differential intracellular polyP levels. Cells grown in high-Pi medium (50 mM) maintained high polyP levels in stationary phase, while those grown in sufficient Pi medium (5 mM) degraded it. Through a RNA-seq approach, comparison of transcriptional profiles of H. seropedicae cultures revealed that 670 genes were differentially expressed between both Pi growth conditions, with 57% repressed and 43% induced in the high Pi condition. Molecular and physiological analyses revealed that aspects related to Pi metabolism, biosynthesis of flagella and chemotaxis, energy production, and polyhydroxybutyrate metabolism were induced in the high-Pi condition, while those involved in adhesion and stress response were repressed. The present study demonstrated that variations in environmental Pi concentration affect H. seropedicae traits related to survival and other important physiological characteristics. Since environmental conditions can influence the effectiveness of the plant growth-promoting bacteria, enhancement of bacterial robustness to withstand different stressful situations is an interesting challenge. The obtained data could serve not only to understand the bacterial behavior in respect to changes in rhizospheric Pi gradients but also as a base to design strategies to improve different bacterial features focusing on biotechnological and/or agricultural purposes.
publishDate 2021
dc.date.none.fl_str_mv 2021-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184370
Grillo Puertas, Mariana; Villegas, Josefina Maria; Pankievicz, Vânia C. S.; Tadra-Sfeir, Michelle Z.; Teles Mota, Francisco J.; et al.; Transcriptional Responses of Herbaspirillum seropedicae to Environmental Phosphate Concentration; Frontiers Media; Frontiers in Microbiology; 12; 6-2021; 1-15
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184370
identifier_str_mv Grillo Puertas, Mariana; Villegas, Josefina Maria; Pankievicz, Vânia C. S.; Tadra-Sfeir, Michelle Z.; Teles Mota, Francisco J.; et al.; Transcriptional Responses of Herbaspirillum seropedicae to Environmental Phosphate Concentration; Frontiers Media; Frontiers in Microbiology; 12; 6-2021; 1-15
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2021.666277
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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