The arginine deiminase pathway in the wine lactic acid bacterium Lactobacillus hilgardii X1B: Structural and functional study of the arcABC genes

Autores
Arena, Mario Eduardo; Manca, Maria Cristina; Muñoz, Rosario
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The genes implicated in the catabolism of the amino acid arginine by Lactobacillus hilgardii X1B were investigated to assess the potential for formation of ethyl carbamate precursors in wine. L. hilgardii X1B can use arginine via the arginine deiminase pathway. The complete nucleotide sequence of the arc genes involved in this pathway has been determined. They are clustered in an operon-like structure in the order arcABC. No evidence was found for the presence of a homologue of the arcD gene, coding for the arginine/ornithine antiporter. The arc genes have been expressed in Escherichia coli resulting in arginine deiminase (ArcA), ornithine carbamoyltransfera (ArcB) and carbamate kinase (ArcC) activities. The results indicate the need for caution in the selection of lactic acid bacteria for conducting malolactic fermentation in wine since arginine degradation could result in high amounts of ethyl carbamate.
Fil: Arena, Mario Eduardo. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Superior de Investigaciones Científicas. Instituto de Fermentaciones Industriales; España
Fil: Manca, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Muñoz, Rosario. Consejo Superior de Investigaciones Científicas. Instituto de Fermentaciones Industriales; España
Materia
Arginine
Arginine Deiminase Pathway
Ethyl Carbamate
Urethane
Wine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/58670
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The arginine deiminase pathway in the wine lactic acid bacterium Lactobacillus hilgardii X1B: Structural and functional study of the arcABC genesArena, Mario EduardoManca, Maria CristinaMuñoz, RosarioArginineArginine Deiminase PathwayEthyl CarbamateUrethaneWinehttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2The genes implicated in the catabolism of the amino acid arginine by Lactobacillus hilgardii X1B were investigated to assess the potential for formation of ethyl carbamate precursors in wine. L. hilgardii X1B can use arginine via the arginine deiminase pathway. The complete nucleotide sequence of the arc genes involved in this pathway has been determined. They are clustered in an operon-like structure in the order arcABC. No evidence was found for the presence of a homologue of the arcD gene, coding for the arginine/ornithine antiporter. The arc genes have been expressed in Escherichia coli resulting in arginine deiminase (ArcA), ornithine carbamoyltransfera (ArcB) and carbamate kinase (ArcC) activities. The results indicate the need for caution in the selection of lactic acid bacteria for conducting malolactic fermentation in wine since arginine degradation could result in high amounts of ethyl carbamate.Fil: Arena, Mario Eduardo. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Superior de Investigaciones Científicas. Instituto de Fermentaciones Industriales; EspañaFil: Manca, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Muñoz, Rosario. Consejo Superior de Investigaciones Científicas. Instituto de Fermentaciones Industriales; EspañaElsevier Science2002-11-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/58670Arena, Mario Eduardo; Manca, Maria Cristina; Muñoz, Rosario; The arginine deiminase pathway in the wine lactic acid bacterium Lactobacillus hilgardii X1B: Structural and functional study of the arcABC genes; Elsevier Science; Gene; 301; 1-2; 13-11-2002; 61-660378-11191879-0038CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/S0378-1119(02)01083-1info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378111902010831info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:29Zoai:ri.conicet.gov.ar:11336/58670instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:30.17CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The arginine deiminase pathway in the wine lactic acid bacterium Lactobacillus hilgardii X1B: Structural and functional study of the arcABC genes
title The arginine deiminase pathway in the wine lactic acid bacterium Lactobacillus hilgardii X1B: Structural and functional study of the arcABC genes
spellingShingle The arginine deiminase pathway in the wine lactic acid bacterium Lactobacillus hilgardii X1B: Structural and functional study of the arcABC genes
Arena, Mario Eduardo
Arginine
Arginine Deiminase Pathway
Ethyl Carbamate
Urethane
Wine
title_short The arginine deiminase pathway in the wine lactic acid bacterium Lactobacillus hilgardii X1B: Structural and functional study of the arcABC genes
title_full The arginine deiminase pathway in the wine lactic acid bacterium Lactobacillus hilgardii X1B: Structural and functional study of the arcABC genes
title_fullStr The arginine deiminase pathway in the wine lactic acid bacterium Lactobacillus hilgardii X1B: Structural and functional study of the arcABC genes
title_full_unstemmed The arginine deiminase pathway in the wine lactic acid bacterium Lactobacillus hilgardii X1B: Structural and functional study of the arcABC genes
title_sort The arginine deiminase pathway in the wine lactic acid bacterium Lactobacillus hilgardii X1B: Structural and functional study of the arcABC genes
dc.creator.none.fl_str_mv Arena, Mario Eduardo
Manca, Maria Cristina
Muñoz, Rosario
author Arena, Mario Eduardo
author_facet Arena, Mario Eduardo
Manca, Maria Cristina
Muñoz, Rosario
author_role author
author2 Manca, Maria Cristina
Muñoz, Rosario
author2_role author
author
dc.subject.none.fl_str_mv Arginine
Arginine Deiminase Pathway
Ethyl Carbamate
Urethane
Wine
topic Arginine
Arginine Deiminase Pathway
Ethyl Carbamate
Urethane
Wine
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The genes implicated in the catabolism of the amino acid arginine by Lactobacillus hilgardii X1B were investigated to assess the potential for formation of ethyl carbamate precursors in wine. L. hilgardii X1B can use arginine via the arginine deiminase pathway. The complete nucleotide sequence of the arc genes involved in this pathway has been determined. They are clustered in an operon-like structure in the order arcABC. No evidence was found for the presence of a homologue of the arcD gene, coding for the arginine/ornithine antiporter. The arc genes have been expressed in Escherichia coli resulting in arginine deiminase (ArcA), ornithine carbamoyltransfera (ArcB) and carbamate kinase (ArcC) activities. The results indicate the need for caution in the selection of lactic acid bacteria for conducting malolactic fermentation in wine since arginine degradation could result in high amounts of ethyl carbamate.
Fil: Arena, Mario Eduardo. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Superior de Investigaciones Científicas. Instituto de Fermentaciones Industriales; España
Fil: Manca, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Muñoz, Rosario. Consejo Superior de Investigaciones Científicas. Instituto de Fermentaciones Industriales; España
description The genes implicated in the catabolism of the amino acid arginine by Lactobacillus hilgardii X1B were investigated to assess the potential for formation of ethyl carbamate precursors in wine. L. hilgardii X1B can use arginine via the arginine deiminase pathway. The complete nucleotide sequence of the arc genes involved in this pathway has been determined. They are clustered in an operon-like structure in the order arcABC. No evidence was found for the presence of a homologue of the arcD gene, coding for the arginine/ornithine antiporter. The arc genes have been expressed in Escherichia coli resulting in arginine deiminase (ArcA), ornithine carbamoyltransfera (ArcB) and carbamate kinase (ArcC) activities. The results indicate the need for caution in the selection of lactic acid bacteria for conducting malolactic fermentation in wine since arginine degradation could result in high amounts of ethyl carbamate.
publishDate 2002
dc.date.none.fl_str_mv 2002-11-13
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/58670
Arena, Mario Eduardo; Manca, Maria Cristina; Muñoz, Rosario; The arginine deiminase pathway in the wine lactic acid bacterium Lactobacillus hilgardii X1B: Structural and functional study of the arcABC genes; Elsevier Science; Gene; 301; 1-2; 13-11-2002; 61-66
0378-1119
1879-0038
CONICET Digital
CONICET
url http://hdl.handle.net/11336/58670
identifier_str_mv Arena, Mario Eduardo; Manca, Maria Cristina; Muñoz, Rosario; The arginine deiminase pathway in the wine lactic acid bacterium Lactobacillus hilgardii X1B: Structural and functional study of the arcABC genes; Elsevier Science; Gene; 301; 1-2; 13-11-2002; 61-66
0378-1119
1879-0038
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/S0378-1119(02)01083-1
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378111902010831
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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