Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves

Autores
Mazorra Morales, Luis Miguel; Senn, María Eugenia; Gergoff Grozeff, Gustavo Esteban; Fanello, Diego Darío; Carrión, Cristian Antonio; Núñez, Miriam; Bishop, Gerard James; Bartoli, Carlos Guillermo
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plant steroid hormones brassinosteroids (BRs) and the gaseous hormone ethylene (ET) alter the ascorbic acid - glutathione (AA-GSH) levels in tomato (Solanum lycopersicum L.) plants. The interaction of these hormones in regulating antioxidant metabolism is however unknown. The combined use of genetics (BR mutants) and chemical application (BR/ET-related chemicals) shows that BRs and ET signalling pathways interact, to regulate leaf AA content and synthesis. BR deficient (dx) leaves display low total AA but BR-accumulating (35S:D) leaves show normal total AA content. Leaves with either BR levels lower or higher than wild type plants showed a higher oxidised AA redox state. The activity of L galactono-1,4-lactone dehydrogenase (L-GalLDH), the mitochondrial enzyme that catalyses the last step in AA synthesis is lower in dx and higher in 35S:D plants. BR-deficient mutants show higher ET production but it is restored to normal levels when BR content is increased in 35S:D plants. Suppression of ET signalling using 1-methylcyclopropene in dx and 35S:D plants restored leaf AA content and L-GalLDH activity, to the values observed in wild type. The suppression of ET action in dx and 35S:D leaves leads to the respective decreasing and increasing respiration, indicating an opposite response compared to AA synthesis. This inverse relationship is lacking in ET suppressed dx plants in response to external BRs. The modifications in the in vivo activity of L-GalLDH activity do not correlate with changes in the level of the enzyme. Taken together, these data suggest that ET suppresses and BRs promote AA synthesis and accumulation.
Fil: Mazorra Morales, Luis Miguel. Instituto Nacional de Ciencias Agrícolas; Cuba. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Senn, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Gergoff Grozeff, Gustavo Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Fanello, Diego Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Carrión, Cristian Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Núñez, Miriam. Instituto Nacional de Ciencias Agrícolas; Cuba
Fil: Bishop, Gerard James. Imperial College London; Reino Unido
Fil: Bartoli, Carlos Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Materia
Antioxidants
Ascorbic acid
Brassinosteroids
Ethylene
Leaves
Respiration
Tomato
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/101900
Acceder
id CONICETDig_c0b92ef5ca6c50029fa15978dabbc25a
oai_identifier_str oai:ri.conicet.gov.ar:11336/101900
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leavesMazorra Morales, Luis MiguelSenn, María EugeniaGergoff Grozeff, Gustavo EstebanFanello, Diego DaríoCarrión, Cristian AntonioNúñez, MiriamBishop, Gerard JamesBartoli, Carlos GuillermoAntioxidantsAscorbic acidBrassinosteroidsEthyleneLeavesRespirationTomatohttps://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4Plant steroid hormones brassinosteroids (BRs) and the gaseous hormone ethylene (ET) alter the ascorbic acid - glutathione (AA-GSH) levels in tomato (Solanum lycopersicum L.) plants. The interaction of these hormones in regulating antioxidant metabolism is however unknown. The combined use of genetics (BR mutants) and chemical application (BR/ET-related chemicals) shows that BRs and ET signalling pathways interact, to regulate leaf AA content and synthesis. BR deficient (dx) leaves display low total AA but BR-accumulating (35S:D) leaves show normal total AA content. Leaves with either BR levels lower or higher than wild type plants showed a higher oxidised AA redox state. The activity of L galactono-1,4-lactone dehydrogenase (L-GalLDH), the mitochondrial enzyme that catalyses the last step in AA synthesis is lower in dx and higher in 35S:D plants. BR-deficient mutants show higher ET production but it is restored to normal levels when BR content is increased in 35S:D plants. Suppression of ET signalling using 1-methylcyclopropene in dx and 35S:D plants restored leaf AA content and L-GalLDH activity, to the values observed in wild type. The suppression of ET action in dx and 35S:D leaves leads to the respective decreasing and increasing respiration, indicating an opposite response compared to AA synthesis. This inverse relationship is lacking in ET suppressed dx plants in response to external BRs. The modifications in the in vivo activity of L-GalLDH activity do not correlate with changes in the level of the enzyme. Taken together, these data suggest that ET suppresses and BRs promote AA synthesis and accumulation.Fil: Mazorra Morales, Luis Miguel. Instituto Nacional de Ciencias Agrícolas; Cuba. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Senn, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Gergoff Grozeff, Gustavo Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Fanello, Diego Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Carrión, Cristian Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: Núñez, Miriam. Instituto Nacional de Ciencias Agrícolas; CubaFil: Bishop, Gerard James. Imperial College London; Reino UnidoFil: Bartoli, Carlos Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaElsevier France-editions Scientifiques Medicales Elsevier2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/101900Mazorra Morales, Luis Miguel; Senn, María Eugenia; Gergoff Grozeff, Gustavo Esteban; Fanello, Diego Darío; Carrión, Cristian Antonio; et al.; Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 74; 322; 1-2014; 315-3220981-9428CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0981942813004166info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2013.11.021info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:20Zoai:ri.conicet.gov.ar:11336/101900instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:20.64CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves
title Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves
spellingShingle Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves
Mazorra Morales, Luis Miguel
Antioxidants
Ascorbic acid
Brassinosteroids
Ethylene
Leaves
Respiration
Tomato
title_short Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves
title_full Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves
title_fullStr Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves
title_full_unstemmed Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves
title_sort Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves
dc.creator.none.fl_str_mv Mazorra Morales, Luis Miguel
Senn, María Eugenia
Gergoff Grozeff, Gustavo Esteban
Fanello, Diego Darío
Carrión, Cristian Antonio
Núñez, Miriam
Bishop, Gerard James
Bartoli, Carlos Guillermo
author Mazorra Morales, Luis Miguel
author_facet Mazorra Morales, Luis Miguel
Senn, María Eugenia
Gergoff Grozeff, Gustavo Esteban
Fanello, Diego Darío
Carrión, Cristian Antonio
Núñez, Miriam
Bishop, Gerard James
Bartoli, Carlos Guillermo
author_role author
author2 Senn, María Eugenia
Gergoff Grozeff, Gustavo Esteban
Fanello, Diego Darío
Carrión, Cristian Antonio
Núñez, Miriam
Bishop, Gerard James
Bartoli, Carlos Guillermo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Antioxidants
Ascorbic acid
Brassinosteroids
Ethylene
Leaves
Respiration
Tomato
topic Antioxidants
Ascorbic acid
Brassinosteroids
Ethylene
Leaves
Respiration
Tomato
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Plant steroid hormones brassinosteroids (BRs) and the gaseous hormone ethylene (ET) alter the ascorbic acid - glutathione (AA-GSH) levels in tomato (Solanum lycopersicum L.) plants. The interaction of these hormones in regulating antioxidant metabolism is however unknown. The combined use of genetics (BR mutants) and chemical application (BR/ET-related chemicals) shows that BRs and ET signalling pathways interact, to regulate leaf AA content and synthesis. BR deficient (dx) leaves display low total AA but BR-accumulating (35S:D) leaves show normal total AA content. Leaves with either BR levels lower or higher than wild type plants showed a higher oxidised AA redox state. The activity of L galactono-1,4-lactone dehydrogenase (L-GalLDH), the mitochondrial enzyme that catalyses the last step in AA synthesis is lower in dx and higher in 35S:D plants. BR-deficient mutants show higher ET production but it is restored to normal levels when BR content is increased in 35S:D plants. Suppression of ET signalling using 1-methylcyclopropene in dx and 35S:D plants restored leaf AA content and L-GalLDH activity, to the values observed in wild type. The suppression of ET action in dx and 35S:D leaves leads to the respective decreasing and increasing respiration, indicating an opposite response compared to AA synthesis. This inverse relationship is lacking in ET suppressed dx plants in response to external BRs. The modifications in the in vivo activity of L-GalLDH activity do not correlate with changes in the level of the enzyme. Taken together, these data suggest that ET suppresses and BRs promote AA synthesis and accumulation.
Fil: Mazorra Morales, Luis Miguel. Instituto Nacional de Ciencias Agrícolas; Cuba. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Senn, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Gergoff Grozeff, Gustavo Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Fanello, Diego Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Carrión, Cristian Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: Núñez, Miriam. Instituto Nacional de Ciencias Agrícolas; Cuba
Fil: Bishop, Gerard James. Imperial College London; Reino Unido
Fil: Bartoli, Carlos Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
description Plant steroid hormones brassinosteroids (BRs) and the gaseous hormone ethylene (ET) alter the ascorbic acid - glutathione (AA-GSH) levels in tomato (Solanum lycopersicum L.) plants. The interaction of these hormones in regulating antioxidant metabolism is however unknown. The combined use of genetics (BR mutants) and chemical application (BR/ET-related chemicals) shows that BRs and ET signalling pathways interact, to regulate leaf AA content and synthesis. BR deficient (dx) leaves display low total AA but BR-accumulating (35S:D) leaves show normal total AA content. Leaves with either BR levels lower or higher than wild type plants showed a higher oxidised AA redox state. The activity of L galactono-1,4-lactone dehydrogenase (L-GalLDH), the mitochondrial enzyme that catalyses the last step in AA synthesis is lower in dx and higher in 35S:D plants. BR-deficient mutants show higher ET production but it is restored to normal levels when BR content is increased in 35S:D plants. Suppression of ET signalling using 1-methylcyclopropene in dx and 35S:D plants restored leaf AA content and L-GalLDH activity, to the values observed in wild type. The suppression of ET action in dx and 35S:D leaves leads to the respective decreasing and increasing respiration, indicating an opposite response compared to AA synthesis. This inverse relationship is lacking in ET suppressed dx plants in response to external BRs. The modifications in the in vivo activity of L-GalLDH activity do not correlate with changes in the level of the enzyme. Taken together, these data suggest that ET suppresses and BRs promote AA synthesis and accumulation.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/101900
Mazorra Morales, Luis Miguel; Senn, María Eugenia; Gergoff Grozeff, Gustavo Esteban; Fanello, Diego Darío; Carrión, Cristian Antonio; et al.; Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 74; 322; 1-2014; 315-322
0981-9428
CONICET Digital
CONICET
url http://hdl.handle.net/11336/101900
identifier_str_mv Mazorra Morales, Luis Miguel; Senn, María Eugenia; Gergoff Grozeff, Gustavo Esteban; Fanello, Diego Darío; Carrión, Cristian Antonio; et al.; Impact of brassinosteroids and ethylene on ascorbic acid accumulation in tomato leaves; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 74; 322; 1-2014; 315-322
0981-9428
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0981942813004166
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plaphy.2013.11.021
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier France-editions Scientifiques Medicales Elsevier
publisher.none.fl_str_mv Elsevier France-editions Scientifiques Medicales Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613805606174720
score 13.070432

Publicaciones similares