The Nuclear Pore Complex acts as a hub for pri-miRNA transcription and processing in plants

Autores
Gonzalo, Lucía; Gagliardi, Delfina; Zlauvinen, Camila; Gulanicz, Tomasz; Arce, Agustín Lucas; Fernández, Josefina; Cambiagno, Damián Alejandro; Zienkiewicz, Agnieszka; Jarmolowski, Artur; Manavella, Pablo Andrés
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The regulation of miRNA biogenesis and movement is essential for plant development and environmental responses. HASTY (HST), a karyopherin protein, has been implicated in miRNA biogenesis and movement, though its role in non-cell-autonomous miRNA movement remains unclear. Through a genetic screen, we identified that mutations in the HAWAIIAN SKIRT (HWS) gene suppress the developmental defects of hst mutants by restoring miRNA movement. Our findings show that HWS interacts with nuclear transport factors and nuclear pore complex (NPC) components, including NUP1, positioning HWS as a regulator of miRNA nuclear export. Using microscopy and fluorescence in situ hybridization, we showed that pri-miRNA transcription, and likely their co-transcriptional processing, occurs at the nuclear pore. Notably, we uncovered an antagonistic relationship between HST and HWS in regulating MIRNA transcription at the NPC and AGO1 loading, which could explain the observed changes in miRNA movement. HST promotes the association of MIRNA loci with the NPC, likely facilitating co-transcriptional processing, while HWS negatively regulates this process by targeting MEDIATOR complex subunits to degradation detaching, consequently, the processing complex from the NPC. Furthermore, our data provides evidence of spatial coordination of miRNA transcription, biogenesis, and movement highlighting a novel role for the NPC in the miRNA pathway.
Fil: Gonzalo, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Gagliardi, Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Zlauvinen, Camila. Consejo Superior de Investigaciones Cientificas. Instituto de Hortofruticultura Subtropical y Mediterránea la Mayora.; España
Fil: Gulanicz, Tomasz. Nicolaus Copernicus University in Toruń; Polonia
Fil: Arce, Agustín Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Fernández, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Cambiagno, Damián Alejandro. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Estudios Agropecuarios.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Zienkiewicz, Agnieszka. Nicolaus Copernicus University in Toruń; Polonia
Fil: Jarmolowski, Artur. Adam Mickiewicz University; Polonia
Fil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Materia
ARABIDOPSIS
NUCLEAR PORE COMPLEX
HASTY
HAWAIIAN SKIRT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/260426
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/260426
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The Nuclear Pore Complex acts as a hub for pri-miRNA transcription and processing in plantsGonzalo, LucíaGagliardi, DelfinaZlauvinen, CamilaGulanicz, TomaszArce, Agustín LucasFernández, JosefinaCambiagno, Damián AlejandroZienkiewicz, AgnieszkaJarmolowski, ArturManavella, Pablo AndrésARABIDOPSISNUCLEAR PORE COMPLEXHASTYHAWAIIAN SKIRThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The regulation of miRNA biogenesis and movement is essential for plant development and environmental responses. HASTY (HST), a karyopherin protein, has been implicated in miRNA biogenesis and movement, though its role in non-cell-autonomous miRNA movement remains unclear. Through a genetic screen, we identified that mutations in the HAWAIIAN SKIRT (HWS) gene suppress the developmental defects of hst mutants by restoring miRNA movement. Our findings show that HWS interacts with nuclear transport factors and nuclear pore complex (NPC) components, including NUP1, positioning HWS as a regulator of miRNA nuclear export. Using microscopy and fluorescence in situ hybridization, we showed that pri-miRNA transcription, and likely their co-transcriptional processing, occurs at the nuclear pore. Notably, we uncovered an antagonistic relationship between HST and HWS in regulating MIRNA transcription at the NPC and AGO1 loading, which could explain the observed changes in miRNA movement. HST promotes the association of MIRNA loci with the NPC, likely facilitating co-transcriptional processing, while HWS negatively regulates this process by targeting MEDIATOR complex subunits to degradation detaching, consequently, the processing complex from the NPC. Furthermore, our data provides evidence of spatial coordination of miRNA transcription, biogenesis, and movement highlighting a novel role for the NPC in the miRNA pathway.Fil: Gonzalo, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Gagliardi, Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Zlauvinen, Camila. Consejo Superior de Investigaciones Cientificas. Instituto de Hortofruticultura Subtropical y Mediterránea la Mayora.; EspañaFil: Gulanicz, Tomasz. Nicolaus Copernicus University in Toruń; PoloniaFil: Arce, Agustín Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Fernández, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Cambiagno, Damián Alejandro. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Estudios Agropecuarios.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Zienkiewicz, Agnieszka. Nicolaus Copernicus University in Toruń; PoloniaFil: Jarmolowski, Artur. Adam Mickiewicz University; PoloniaFil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaCold Spring Harbor Laboratory Press2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/260426Gonzalo, Lucía; Gagliardi, Delfina; Zlauvinen, Camila; Gulanicz, Tomasz; Arce, Agustín Lucas; et al.; The Nuclear Pore Complex acts as a hub for pri-miRNA transcription and processing in plants; Cold Spring Harbor Laboratory Press; bioRxiv; 10-2024; 1-412692-8205CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1101/2024.10.24.620027info:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/2024.10.24.620027v1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:07:02Zoai:ri.conicet.gov.ar:11336/260426instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:07:03.071CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Nuclear Pore Complex acts as a hub for pri-miRNA transcription and processing in plants
title The Nuclear Pore Complex acts as a hub for pri-miRNA transcription and processing in plants
spellingShingle The Nuclear Pore Complex acts as a hub for pri-miRNA transcription and processing in plants
Gonzalo, Lucía
ARABIDOPSIS
NUCLEAR PORE COMPLEX
HASTY
HAWAIIAN SKIRT
title_short The Nuclear Pore Complex acts as a hub for pri-miRNA transcription and processing in plants
title_full The Nuclear Pore Complex acts as a hub for pri-miRNA transcription and processing in plants
title_fullStr The Nuclear Pore Complex acts as a hub for pri-miRNA transcription and processing in plants
title_full_unstemmed The Nuclear Pore Complex acts as a hub for pri-miRNA transcription and processing in plants
title_sort The Nuclear Pore Complex acts as a hub for pri-miRNA transcription and processing in plants
dc.creator.none.fl_str_mv Gonzalo, Lucía
Gagliardi, Delfina
Zlauvinen, Camila
Gulanicz, Tomasz
Arce, Agustín Lucas
Fernández, Josefina
Cambiagno, Damián Alejandro
Zienkiewicz, Agnieszka
Jarmolowski, Artur
Manavella, Pablo Andrés
author Gonzalo, Lucía
author_facet Gonzalo, Lucía
Gagliardi, Delfina
Zlauvinen, Camila
Gulanicz, Tomasz
Arce, Agustín Lucas
Fernández, Josefina
Cambiagno, Damián Alejandro
Zienkiewicz, Agnieszka
Jarmolowski, Artur
Manavella, Pablo Andrés
author_role author
author2 Gagliardi, Delfina
Zlauvinen, Camila
Gulanicz, Tomasz
Arce, Agustín Lucas
Fernández, Josefina
Cambiagno, Damián Alejandro
Zienkiewicz, Agnieszka
Jarmolowski, Artur
Manavella, Pablo Andrés
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ARABIDOPSIS
NUCLEAR PORE COMPLEX
HASTY
HAWAIIAN SKIRT
topic ARABIDOPSIS
NUCLEAR PORE COMPLEX
HASTY
HAWAIIAN SKIRT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The regulation of miRNA biogenesis and movement is essential for plant development and environmental responses. HASTY (HST), a karyopherin protein, has been implicated in miRNA biogenesis and movement, though its role in non-cell-autonomous miRNA movement remains unclear. Through a genetic screen, we identified that mutations in the HAWAIIAN SKIRT (HWS) gene suppress the developmental defects of hst mutants by restoring miRNA movement. Our findings show that HWS interacts with nuclear transport factors and nuclear pore complex (NPC) components, including NUP1, positioning HWS as a regulator of miRNA nuclear export. Using microscopy and fluorescence in situ hybridization, we showed that pri-miRNA transcription, and likely their co-transcriptional processing, occurs at the nuclear pore. Notably, we uncovered an antagonistic relationship between HST and HWS in regulating MIRNA transcription at the NPC and AGO1 loading, which could explain the observed changes in miRNA movement. HST promotes the association of MIRNA loci with the NPC, likely facilitating co-transcriptional processing, while HWS negatively regulates this process by targeting MEDIATOR complex subunits to degradation detaching, consequently, the processing complex from the NPC. Furthermore, our data provides evidence of spatial coordination of miRNA transcription, biogenesis, and movement highlighting a novel role for the NPC in the miRNA pathway.
Fil: Gonzalo, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Gagliardi, Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Zlauvinen, Camila. Consejo Superior de Investigaciones Cientificas. Instituto de Hortofruticultura Subtropical y Mediterránea la Mayora.; España
Fil: Gulanicz, Tomasz. Nicolaus Copernicus University in Toruń; Polonia
Fil: Arce, Agustín Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Fernández, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Cambiagno, Damián Alejandro. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Estudios Agropecuarios.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Zienkiewicz, Agnieszka. Nicolaus Copernicus University in Toruń; Polonia
Fil: Jarmolowski, Artur. Adam Mickiewicz University; Polonia
Fil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
description The regulation of miRNA biogenesis and movement is essential for plant development and environmental responses. HASTY (HST), a karyopherin protein, has been implicated in miRNA biogenesis and movement, though its role in non-cell-autonomous miRNA movement remains unclear. Through a genetic screen, we identified that mutations in the HAWAIIAN SKIRT (HWS) gene suppress the developmental defects of hst mutants by restoring miRNA movement. Our findings show that HWS interacts with nuclear transport factors and nuclear pore complex (NPC) components, including NUP1, positioning HWS as a regulator of miRNA nuclear export. Using microscopy and fluorescence in situ hybridization, we showed that pri-miRNA transcription, and likely their co-transcriptional processing, occurs at the nuclear pore. Notably, we uncovered an antagonistic relationship between HST and HWS in regulating MIRNA transcription at the NPC and AGO1 loading, which could explain the observed changes in miRNA movement. HST promotes the association of MIRNA loci with the NPC, likely facilitating co-transcriptional processing, while HWS negatively regulates this process by targeting MEDIATOR complex subunits to degradation detaching, consequently, the processing complex from the NPC. Furthermore, our data provides evidence of spatial coordination of miRNA transcription, biogenesis, and movement highlighting a novel role for the NPC in the miRNA pathway.
publishDate 2024
dc.date.none.fl_str_mv 2024-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/260426
Gonzalo, Lucía; Gagliardi, Delfina; Zlauvinen, Camila; Gulanicz, Tomasz; Arce, Agustín Lucas; et al.; The Nuclear Pore Complex acts as a hub for pri-miRNA transcription and processing in plants; Cold Spring Harbor Laboratory Press; bioRxiv; 10-2024; 1-41
2692-8205
CONICET Digital
CONICET
url http://hdl.handle.net/11336/260426
identifier_str_mv Gonzalo, Lucía; Gagliardi, Delfina; Zlauvinen, Camila; Gulanicz, Tomasz; Arce, Agustín Lucas; et al.; The Nuclear Pore Complex acts as a hub for pri-miRNA transcription and processing in plants; Cold Spring Harbor Laboratory Press; bioRxiv; 10-2024; 1-41
2692-8205
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1101/2024.10.24.620027
info:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/2024.10.24.620027v1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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