Study of cytokine induced neuropathology by high resolution proton NMR spectroscopy of rat urine

Autores
Griffin, Julian L.; Anthony, Daniel C.; Campbell, Sandra J.; Gauldie, Jack; Pitossi, Fernando Juan; Styles, Peter; Sibson, Nicola R.
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Multiple sclerosis is a major cause of non-traumatic neurological disability. The identification of markers that differentiate disease progression is critical to effective therapy. A combination of NMR spectroscopic metabolic profiling of urine and statistical pattern recognition was used to detect focal inflammatory central nervous system (CNS) lesions induced by microinjection of a replication-deficient recombinant adenovirus expressing TNF-alpha or IL1-beta cDNA into the brains of Wistar rats. These animals were compared with a group of naïve rats and a group of animals injected with an equivalent null adenovirus. Urine samples were collected 7 days after adenovirus injection, when the inflammatory lesion is maximally active. Principal components analysis and Partial Least Squares-Discriminate analysis of the urine (1)H NMR spectra revealed significant differences between each of the cytokine adenovirus groups and the control groups; for the TNF-alpha group the main differences lay in citrate and succinate, while for the IL-1beta group the predominant changes occurred in leucine, isoleucine, valine and myo-inositol. Thus, we can identify urinary metabolic vectors that not only separate rats with inflammatory lesions in the brain from control animals, but also distinguish between different types of CNS inflammatory lesions.
Fil: Griffin, Julian L.. University of Cambridge; Reino Unido
Fil: Anthony, Daniel C.. University of Southampton; Reino Unido
Fil: Campbell, Sandra J.. University of Southampton; Reino Unido
Fil: Gauldie, Jack. McMaster University; Canadá
Fil: Pitossi, Fernando Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Styles, Peter. McMaster University; Canadá
Fil: Sibson, Nicola R.. University of Oxford; Reino Unido
Materia
Multiple Sclerosis
Metabolomics
Metabonomics
Pattern Recognition
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43019
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Study of cytokine induced neuropathology by high resolution proton NMR spectroscopy of rat urineGriffin, Julian L.Anthony, Daniel C.Campbell, Sandra J.Gauldie, JackPitossi, Fernando JuanStyles, PeterSibson, Nicola R.Multiple SclerosisMetabolomicsMetabonomicsPattern Recognitionhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Multiple sclerosis is a major cause of non-traumatic neurological disability. The identification of markers that differentiate disease progression is critical to effective therapy. A combination of NMR spectroscopic metabolic profiling of urine and statistical pattern recognition was used to detect focal inflammatory central nervous system (CNS) lesions induced by microinjection of a replication-deficient recombinant adenovirus expressing TNF-alpha or IL1-beta cDNA into the brains of Wistar rats. These animals were compared with a group of naïve rats and a group of animals injected with an equivalent null adenovirus. Urine samples were collected 7 days after adenovirus injection, when the inflammatory lesion is maximally active. Principal components analysis and Partial Least Squares-Discriminate analysis of the urine (1)H NMR spectra revealed significant differences between each of the cytokine adenovirus groups and the control groups; for the TNF-alpha group the main differences lay in citrate and succinate, while for the IL-1beta group the predominant changes occurred in leucine, isoleucine, valine and myo-inositol. Thus, we can identify urinary metabolic vectors that not only separate rats with inflammatory lesions in the brain from control animals, but also distinguish between different types of CNS inflammatory lesions.Fil: Griffin, Julian L.. University of Cambridge; Reino UnidoFil: Anthony, Daniel C.. University of Southampton; Reino UnidoFil: Campbell, Sandra J.. University of Southampton; Reino UnidoFil: Gauldie, Jack. McMaster University; CanadáFil: Pitossi, Fernando Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Styles, Peter. McMaster University; CanadáFil: Sibson, Nicola R.. University of Oxford; Reino UnidoElsevier Science2004-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43019Griffin, Julian L.; Anthony, Daniel C.; Campbell, Sandra J.; Gauldie, Jack; Pitossi, Fernando Juan; et al.; Study of cytokine induced neuropathology by high resolution proton NMR spectroscopy of rat urine; Elsevier Science; FEBS Letters; 568; 1-3; 6-2004; 49-540014-57931873-3468CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/j.febslet.2004.04.096info:eu-repo/semantics/altIdentifier/doi/10.1016/j.febslet.2004.04.096info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:59Zoai:ri.conicet.gov.ar:11336/43019instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:59.717CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Study of cytokine induced neuropathology by high resolution proton NMR spectroscopy of rat urine
title Study of cytokine induced neuropathology by high resolution proton NMR spectroscopy of rat urine
spellingShingle Study of cytokine induced neuropathology by high resolution proton NMR spectroscopy of rat urine
Griffin, Julian L.
Multiple Sclerosis
Metabolomics
Metabonomics
Pattern Recognition
title_short Study of cytokine induced neuropathology by high resolution proton NMR spectroscopy of rat urine
title_full Study of cytokine induced neuropathology by high resolution proton NMR spectroscopy of rat urine
title_fullStr Study of cytokine induced neuropathology by high resolution proton NMR spectroscopy of rat urine
title_full_unstemmed Study of cytokine induced neuropathology by high resolution proton NMR spectroscopy of rat urine
title_sort Study of cytokine induced neuropathology by high resolution proton NMR spectroscopy of rat urine
dc.creator.none.fl_str_mv Griffin, Julian L.
Anthony, Daniel C.
Campbell, Sandra J.
Gauldie, Jack
Pitossi, Fernando Juan
Styles, Peter
Sibson, Nicola R.
author Griffin, Julian L.
author_facet Griffin, Julian L.
Anthony, Daniel C.
Campbell, Sandra J.
Gauldie, Jack
Pitossi, Fernando Juan
Styles, Peter
Sibson, Nicola R.
author_role author
author2 Anthony, Daniel C.
Campbell, Sandra J.
Gauldie, Jack
Pitossi, Fernando Juan
Styles, Peter
Sibson, Nicola R.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Multiple Sclerosis
Metabolomics
Metabonomics
Pattern Recognition
topic Multiple Sclerosis
Metabolomics
Metabonomics
Pattern Recognition
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Multiple sclerosis is a major cause of non-traumatic neurological disability. The identification of markers that differentiate disease progression is critical to effective therapy. A combination of NMR spectroscopic metabolic profiling of urine and statistical pattern recognition was used to detect focal inflammatory central nervous system (CNS) lesions induced by microinjection of a replication-deficient recombinant adenovirus expressing TNF-alpha or IL1-beta cDNA into the brains of Wistar rats. These animals were compared with a group of naïve rats and a group of animals injected with an equivalent null adenovirus. Urine samples were collected 7 days after adenovirus injection, when the inflammatory lesion is maximally active. Principal components analysis and Partial Least Squares-Discriminate analysis of the urine (1)H NMR spectra revealed significant differences between each of the cytokine adenovirus groups and the control groups; for the TNF-alpha group the main differences lay in citrate and succinate, while for the IL-1beta group the predominant changes occurred in leucine, isoleucine, valine and myo-inositol. Thus, we can identify urinary metabolic vectors that not only separate rats with inflammatory lesions in the brain from control animals, but also distinguish between different types of CNS inflammatory lesions.
Fil: Griffin, Julian L.. University of Cambridge; Reino Unido
Fil: Anthony, Daniel C.. University of Southampton; Reino Unido
Fil: Campbell, Sandra J.. University of Southampton; Reino Unido
Fil: Gauldie, Jack. McMaster University; Canadá
Fil: Pitossi, Fernando Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Styles, Peter. McMaster University; Canadá
Fil: Sibson, Nicola R.. University of Oxford; Reino Unido
description Multiple sclerosis is a major cause of non-traumatic neurological disability. The identification of markers that differentiate disease progression is critical to effective therapy. A combination of NMR spectroscopic metabolic profiling of urine and statistical pattern recognition was used to detect focal inflammatory central nervous system (CNS) lesions induced by microinjection of a replication-deficient recombinant adenovirus expressing TNF-alpha or IL1-beta cDNA into the brains of Wistar rats. These animals were compared with a group of naïve rats and a group of animals injected with an equivalent null adenovirus. Urine samples were collected 7 days after adenovirus injection, when the inflammatory lesion is maximally active. Principal components analysis and Partial Least Squares-Discriminate analysis of the urine (1)H NMR spectra revealed significant differences between each of the cytokine adenovirus groups and the control groups; for the TNF-alpha group the main differences lay in citrate and succinate, while for the IL-1beta group the predominant changes occurred in leucine, isoleucine, valine and myo-inositol. Thus, we can identify urinary metabolic vectors that not only separate rats with inflammatory lesions in the brain from control animals, but also distinguish between different types of CNS inflammatory lesions.
publishDate 2004
dc.date.none.fl_str_mv 2004-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43019
Griffin, Julian L.; Anthony, Daniel C.; Campbell, Sandra J.; Gauldie, Jack; Pitossi, Fernando Juan; et al.; Study of cytokine induced neuropathology by high resolution proton NMR spectroscopy of rat urine; Elsevier Science; FEBS Letters; 568; 1-3; 6-2004; 49-54
0014-5793
1873-3468
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43019
identifier_str_mv Griffin, Julian L.; Anthony, Daniel C.; Campbell, Sandra J.; Gauldie, Jack; Pitossi, Fernando Juan; et al.; Study of cytokine induced neuropathology by high resolution proton NMR spectroscopy of rat urine; Elsevier Science; FEBS Letters; 568; 1-3; 6-2004; 49-54
0014-5793
1873-3468
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://febs.onlinelibrary.wiley.com/doi/abs/10.1016/j.febslet.2004.04.096
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.febslet.2004.04.096
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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