Acute exposure to a commercial formulation of Azoxystrobin alters antioxidant enzymes and elicit damage in the aquatic macrophyte Myriophyllum quitense

Autores
Garanzini, Daniela Soledad; Medici, Sandra Karina; Moreyra, Lucía D.; Menone, Mirta Lujan
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Azoxystrobin is a strobilurin of growing concern in aquatic environments because it is the most sold fungicide worldwide, however, the information available about its effect on aquatic non-target organisms is scarce. The objective of the present study was to evaluate potential physiological, biochemical, and genetic effects at environmentally relevant (1–10 μg/L) and elevated (100–500 μg/L) concentrations in the aquatic macrophyte Myriophyllum quitense exposed to the commercial formulation AMISTAR ® . Following an acute 24-h exposure, there were no effects of AMISTAR ® on photosynthetic pigments at any of the concentrations evaluated. Glutathione-S-transferase activity was significantly elevated at 1 and 10 μg/L AZX. Significant decrease of catalase and guaiacol peroxidase activities in plants exposed to 500 μg/L, and to 100 and 500 μg/L, respectively, and an increase in glycolate oxidase activity at 500 μg/L was observed. DNA damage at 100 and 500 μg/L was observed. These data indicate that although environmentally relevant levels of AMISTAR ® did not result cytotoxic, this fungicide was genotoxic, affecting the physiological process of photorespiration and caused oxidative damage at high concentrations. In this sense, it is necessary to explore sub-lethal responses in non-target organisms because some effects could promote further potential long-term biological consequences in a context of repeated pulses of exposure.
Fil: Garanzini, Daniela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Medici, Sandra Karina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fares Taie Instituto de Análisis; Argentina
Fil: Moreyra, Lucía D.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Materia
ANTIOXIDANT ENZYMES
AZOXYSTROBIN
CHLOROPHYLL
MYRIOPHYLLUM QUITENSE
“COMET” ASSAY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/121317
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Acute exposure to a commercial formulation of Azoxystrobin alters antioxidant enzymes and elicit damage in the aquatic macrophyte Myriophyllum quitenseGaranzini, Daniela SoledadMedici, Sandra KarinaMoreyra, Lucía D.Menone, Mirta LujanANTIOXIDANT ENZYMESAZOXYSTROBINCHLOROPHYLLMYRIOPHYLLUM QUITENSE“COMET” ASSAYhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1Azoxystrobin is a strobilurin of growing concern in aquatic environments because it is the most sold fungicide worldwide, however, the information available about its effect on aquatic non-target organisms is scarce. The objective of the present study was to evaluate potential physiological, biochemical, and genetic effects at environmentally relevant (1–10 μg/L) and elevated (100–500 μg/L) concentrations in the aquatic macrophyte Myriophyllum quitense exposed to the commercial formulation AMISTAR ® . Following an acute 24-h exposure, there were no effects of AMISTAR ® on photosynthetic pigments at any of the concentrations evaluated. Glutathione-S-transferase activity was significantly elevated at 1 and 10 μg/L AZX. Significant decrease of catalase and guaiacol peroxidase activities in plants exposed to 500 μg/L, and to 100 and 500 μg/L, respectively, and an increase in glycolate oxidase activity at 500 μg/L was observed. DNA damage at 100 and 500 μg/L was observed. These data indicate that although environmentally relevant levels of AMISTAR ® did not result cytotoxic, this fungicide was genotoxic, affecting the physiological process of photorespiration and caused oxidative damage at high concentrations. In this sense, it is necessary to explore sub-lethal responses in non-target organisms because some effects could promote further potential long-term biological consequences in a context of repeated pulses of exposure.Fil: Garanzini, Daniela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaFil: Medici, Sandra Karina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fares Taie Instituto de Análisis; ArgentinaFil: Moreyra, Lucía D.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaSpringer2019-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/121317Garanzini, Daniela Soledad; Medici, Sandra Karina; Moreyra, Lucía D.; Menone, Mirta Lujan; Acute exposure to a commercial formulation of Azoxystrobin alters antioxidant enzymes and elicit damage in the aquatic macrophyte Myriophyllum quitense; Springer; Physiology and Molecular Biology of Plants; 25; 1; 2-2019; 135-1430971-58940974-0430CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s12298-018-0603-7info:eu-repo/semantics/altIdentifier/doi/10.1007/s12298-018-0603-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:46Zoai:ri.conicet.gov.ar:11336/121317instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:46.278CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Acute exposure to a commercial formulation of Azoxystrobin alters antioxidant enzymes and elicit damage in the aquatic macrophyte Myriophyllum quitense
title Acute exposure to a commercial formulation of Azoxystrobin alters antioxidant enzymes and elicit damage in the aquatic macrophyte Myriophyllum quitense
spellingShingle Acute exposure to a commercial formulation of Azoxystrobin alters antioxidant enzymes and elicit damage in the aquatic macrophyte Myriophyllum quitense
Garanzini, Daniela Soledad
ANTIOXIDANT ENZYMES
AZOXYSTROBIN
CHLOROPHYLL
MYRIOPHYLLUM QUITENSE
“COMET” ASSAY
title_short Acute exposure to a commercial formulation of Azoxystrobin alters antioxidant enzymes and elicit damage in the aquatic macrophyte Myriophyllum quitense
title_full Acute exposure to a commercial formulation of Azoxystrobin alters antioxidant enzymes and elicit damage in the aquatic macrophyte Myriophyllum quitense
title_fullStr Acute exposure to a commercial formulation of Azoxystrobin alters antioxidant enzymes and elicit damage in the aquatic macrophyte Myriophyllum quitense
title_full_unstemmed Acute exposure to a commercial formulation of Azoxystrobin alters antioxidant enzymes and elicit damage in the aquatic macrophyte Myriophyllum quitense
title_sort Acute exposure to a commercial formulation of Azoxystrobin alters antioxidant enzymes and elicit damage in the aquatic macrophyte Myriophyllum quitense
dc.creator.none.fl_str_mv Garanzini, Daniela Soledad
Medici, Sandra Karina
Moreyra, Lucía D.
Menone, Mirta Lujan
author Garanzini, Daniela Soledad
author_facet Garanzini, Daniela Soledad
Medici, Sandra Karina
Moreyra, Lucía D.
Menone, Mirta Lujan
author_role author
author2 Medici, Sandra Karina
Moreyra, Lucía D.
Menone, Mirta Lujan
author2_role author
author
author
dc.subject.none.fl_str_mv ANTIOXIDANT ENZYMES
AZOXYSTROBIN
CHLOROPHYLL
MYRIOPHYLLUM QUITENSE
“COMET” ASSAY
topic ANTIOXIDANT ENZYMES
AZOXYSTROBIN
CHLOROPHYLL
MYRIOPHYLLUM QUITENSE
“COMET” ASSAY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Azoxystrobin is a strobilurin of growing concern in aquatic environments because it is the most sold fungicide worldwide, however, the information available about its effect on aquatic non-target organisms is scarce. The objective of the present study was to evaluate potential physiological, biochemical, and genetic effects at environmentally relevant (1–10 μg/L) and elevated (100–500 μg/L) concentrations in the aquatic macrophyte Myriophyllum quitense exposed to the commercial formulation AMISTAR ® . Following an acute 24-h exposure, there were no effects of AMISTAR ® on photosynthetic pigments at any of the concentrations evaluated. Glutathione-S-transferase activity was significantly elevated at 1 and 10 μg/L AZX. Significant decrease of catalase and guaiacol peroxidase activities in plants exposed to 500 μg/L, and to 100 and 500 μg/L, respectively, and an increase in glycolate oxidase activity at 500 μg/L was observed. DNA damage at 100 and 500 μg/L was observed. These data indicate that although environmentally relevant levels of AMISTAR ® did not result cytotoxic, this fungicide was genotoxic, affecting the physiological process of photorespiration and caused oxidative damage at high concentrations. In this sense, it is necessary to explore sub-lethal responses in non-target organisms because some effects could promote further potential long-term biological consequences in a context of repeated pulses of exposure.
Fil: Garanzini, Daniela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Fil: Medici, Sandra Karina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fares Taie Instituto de Análisis; Argentina
Fil: Moreyra, Lucía D.. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Menone, Mirta Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
description Azoxystrobin is a strobilurin of growing concern in aquatic environments because it is the most sold fungicide worldwide, however, the information available about its effect on aquatic non-target organisms is scarce. The objective of the present study was to evaluate potential physiological, biochemical, and genetic effects at environmentally relevant (1–10 μg/L) and elevated (100–500 μg/L) concentrations in the aquatic macrophyte Myriophyllum quitense exposed to the commercial formulation AMISTAR ® . Following an acute 24-h exposure, there were no effects of AMISTAR ® on photosynthetic pigments at any of the concentrations evaluated. Glutathione-S-transferase activity was significantly elevated at 1 and 10 μg/L AZX. Significant decrease of catalase and guaiacol peroxidase activities in plants exposed to 500 μg/L, and to 100 and 500 μg/L, respectively, and an increase in glycolate oxidase activity at 500 μg/L was observed. DNA damage at 100 and 500 μg/L was observed. These data indicate that although environmentally relevant levels of AMISTAR ® did not result cytotoxic, this fungicide was genotoxic, affecting the physiological process of photorespiration and caused oxidative damage at high concentrations. In this sense, it is necessary to explore sub-lethal responses in non-target organisms because some effects could promote further potential long-term biological consequences in a context of repeated pulses of exposure.
publishDate 2019
dc.date.none.fl_str_mv 2019-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/121317
Garanzini, Daniela Soledad; Medici, Sandra Karina; Moreyra, Lucía D.; Menone, Mirta Lujan; Acute exposure to a commercial formulation of Azoxystrobin alters antioxidant enzymes and elicit damage in the aquatic macrophyte Myriophyllum quitense; Springer; Physiology and Molecular Biology of Plants; 25; 1; 2-2019; 135-143
0971-5894
0974-0430
CONICET Digital
CONICET
url http://hdl.handle.net/11336/121317
identifier_str_mv Garanzini, Daniela Soledad; Medici, Sandra Karina; Moreyra, Lucía D.; Menone, Mirta Lujan; Acute exposure to a commercial formulation of Azoxystrobin alters antioxidant enzymes and elicit damage in the aquatic macrophyte Myriophyllum quitense; Springer; Physiology and Molecular Biology of Plants; 25; 1; 2-2019; 135-143
0971-5894
0974-0430
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s12298-018-0603-7
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12298-018-0603-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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