Long-lasting amphetamine effects over cebtral Angiotensin II responses involve AT1-R

Autores
Piermarini, Maria Josefina; Occhieppo, Victoria Belen; Basmadjian, Osvaldo Martin; Baiardi, Gustavo Carlos; Bregonzio Diaz, Claudia
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Angiotensin II (Ang II), a pleiotropic neuropeptide, plays a critical role in regulating the sympathetic and neuroendocrine systems through the activation of AT 1 receptors (AT1-R). Studies on mammals have shown that administering Ang II through the intracerebroventricular (i.c.v) route increases water and sodium intake, as well as renal sodium excretion. Our group’s previous findings have shown that AT 1-R are involved in behavioural and neurochemical sensitization induced by amphetamine (Amph) administration. Our current aim is to assess the functional and neurochemical response to Ang II, via the AT 1-R activation, in animals that have been previously exposed to Amph. Male Wistar rats (250-320g) were injected intraperitoneally with Amph (2.5mg/kg/day) or saline for 5 days, and implanted with i.c.v. cannulae. Twenty-one days after the last Amph administration, the animals received Ang II (400pmol) i.c.v. First group: the animals were tested in a free choice paradigm for sodium (2% NaCl) and water intake, and sacrificed for Fos immunoreactivity determinations. Second group: urine and plasma samples were collected for electrolytes and plasma renin activity determination. Third group, the animals were tested in the plus maze or the holeboard for anxiety and memory work evaluation, respectively. Previous Amph exposure altered the physiological and behavioral responses described for central Ang II administration. Remarkably, the AT1-R blockade prevented most of these alterations but anxiety. Our results highlight that repeated Amph exposure attenuates the AT1-R functionality in a long-lasting manner, modifying the brain Ang II-induced responses.
Fil: Piermarini, Maria Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Occhieppo, Victoria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Basmadjian, Osvaldo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Baiardi, Gustavo Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Bregonzio Diaz, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Reunión conjunta; LXVIII Reunión anual de la Sociedad Argentina de Investigación Clínica; XXV Jornada anuales de la Sociedad Argentina de Biología; LV Reunión anual de la Asociación Argentina de Farmacología Experimental; VIII Reunión científica regional de la Asociación Argentina de Ciencia y Tecnología de Animlaes de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animlaes de Laboratorio
Materia
ANGIOTENSIN
ANXIETY
WORKING MEMORY
HIDROELECTROLITIC HOMEOSTASIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/259269
Acceder
id CONICETDig_bf6853cd7af3ae780b15d2020bb938fc
oai_identifier_str oai:ri.conicet.gov.ar:11336/259269
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Long-lasting amphetamine effects over cebtral Angiotensin II responses involve AT1-RPiermarini, Maria JosefinaOcchieppo, Victoria BelenBasmadjian, Osvaldo MartinBaiardi, Gustavo CarlosBregonzio Diaz, ClaudiaANGIOTENSINANXIETYWORKING MEMORYHIDROELECTROLITIC HOMEOSTASIShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Angiotensin II (Ang II), a pleiotropic neuropeptide, plays a critical role in regulating the sympathetic and neuroendocrine systems through the activation of AT 1 receptors (AT1-R). Studies on mammals have shown that administering Ang II through the intracerebroventricular (i.c.v) route increases water and sodium intake, as well as renal sodium excretion. Our group’s previous findings have shown that AT 1-R are involved in behavioural and neurochemical sensitization induced by amphetamine (Amph) administration. Our current aim is to assess the functional and neurochemical response to Ang II, via the AT 1-R activation, in animals that have been previously exposed to Amph. Male Wistar rats (250-320g) were injected intraperitoneally with Amph (2.5mg/kg/day) or saline for 5 days, and implanted with i.c.v. cannulae. Twenty-one days after the last Amph administration, the animals received Ang II (400pmol) i.c.v. First group: the animals were tested in a free choice paradigm for sodium (2% NaCl) and water intake, and sacrificed for Fos immunoreactivity determinations. Second group: urine and plasma samples were collected for electrolytes and plasma renin activity determination. Third group, the animals were tested in the plus maze or the holeboard for anxiety and memory work evaluation, respectively. Previous Amph exposure altered the physiological and behavioral responses described for central Ang II administration. Remarkably, the AT1-R blockade prevented most of these alterations but anxiety. Our results highlight that repeated Amph exposure attenuates the AT1-R functionality in a long-lasting manner, modifying the brain Ang II-induced responses.Fil: Piermarini, Maria Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Occhieppo, Victoria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Basmadjian, Osvaldo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaFil: Baiardi, Gustavo Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Bregonzio Diaz, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; ArgentinaReunión conjunta; LXVIII Reunión anual de la Sociedad Argentina de Investigación Clínica; XXV Jornada anuales de la Sociedad Argentina de Biología; LV Reunión anual de la Asociación Argentina de Farmacología Experimental; VIII Reunión científica regional de la Asociación Argentina de Ciencia y Tecnología de Animlaes de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de BiologíaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de Ciencia y Tecnología de Animlaes de LaboratorioFundacion Revista MedicinaLuthy, Isabel AliciaPerez Martinez, Silvina Laura2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/259269Long-lasting amphetamine effects over cebtral Angiotensin II responses involve AT1-R; Reunión conjunta; LXVIII Reunión anual de la Sociedad Argentina de Investigación Clínica; XXV Jornada anuales de la Sociedad Argentina de Biología; LV Reunión anual de la Asociación Argentina de Farmacología Experimental; VIII Reunión científica regional de la Asociación Argentina de Ciencia y Tecnología de Animlaes de Laboratorio; Mar del Plata; Argentina; 2023; 159-1591669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reuniones-anuales-previasNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:57:03Zoai:ri.conicet.gov.ar:11336/259269instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:57:03.959CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Long-lasting amphetamine effects over cebtral Angiotensin II responses involve AT1-R
title Long-lasting amphetamine effects over cebtral Angiotensin II responses involve AT1-R
spellingShingle Long-lasting amphetamine effects over cebtral Angiotensin II responses involve AT1-R
Piermarini, Maria Josefina
ANGIOTENSIN
ANXIETY
WORKING MEMORY
HIDROELECTROLITIC HOMEOSTASIS
title_short Long-lasting amphetamine effects over cebtral Angiotensin II responses involve AT1-R
title_full Long-lasting amphetamine effects over cebtral Angiotensin II responses involve AT1-R
title_fullStr Long-lasting amphetamine effects over cebtral Angiotensin II responses involve AT1-R
title_full_unstemmed Long-lasting amphetamine effects over cebtral Angiotensin II responses involve AT1-R
title_sort Long-lasting amphetamine effects over cebtral Angiotensin II responses involve AT1-R
dc.creator.none.fl_str_mv Piermarini, Maria Josefina
Occhieppo, Victoria Belen
Basmadjian, Osvaldo Martin
Baiardi, Gustavo Carlos
Bregonzio Diaz, Claudia
author Piermarini, Maria Josefina
author_facet Piermarini, Maria Josefina
Occhieppo, Victoria Belen
Basmadjian, Osvaldo Martin
Baiardi, Gustavo Carlos
Bregonzio Diaz, Claudia
author_role author
author2 Occhieppo, Victoria Belen
Basmadjian, Osvaldo Martin
Baiardi, Gustavo Carlos
Bregonzio Diaz, Claudia
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Luthy, Isabel Alicia
Perez Martinez, Silvina Laura
dc.subject.none.fl_str_mv ANGIOTENSIN
ANXIETY
WORKING MEMORY
HIDROELECTROLITIC HOMEOSTASIS
topic ANGIOTENSIN
ANXIETY
WORKING MEMORY
HIDROELECTROLITIC HOMEOSTASIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Angiotensin II (Ang II), a pleiotropic neuropeptide, plays a critical role in regulating the sympathetic and neuroendocrine systems through the activation of AT 1 receptors (AT1-R). Studies on mammals have shown that administering Ang II through the intracerebroventricular (i.c.v) route increases water and sodium intake, as well as renal sodium excretion. Our group’s previous findings have shown that AT 1-R are involved in behavioural and neurochemical sensitization induced by amphetamine (Amph) administration. Our current aim is to assess the functional and neurochemical response to Ang II, via the AT 1-R activation, in animals that have been previously exposed to Amph. Male Wistar rats (250-320g) were injected intraperitoneally with Amph (2.5mg/kg/day) or saline for 5 days, and implanted with i.c.v. cannulae. Twenty-one days after the last Amph administration, the animals received Ang II (400pmol) i.c.v. First group: the animals were tested in a free choice paradigm for sodium (2% NaCl) and water intake, and sacrificed for Fos immunoreactivity determinations. Second group: urine and plasma samples were collected for electrolytes and plasma renin activity determination. Third group, the animals were tested in the plus maze or the holeboard for anxiety and memory work evaluation, respectively. Previous Amph exposure altered the physiological and behavioral responses described for central Ang II administration. Remarkably, the AT1-R blockade prevented most of these alterations but anxiety. Our results highlight that repeated Amph exposure attenuates the AT1-R functionality in a long-lasting manner, modifying the brain Ang II-induced responses.
Fil: Piermarini, Maria Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Occhieppo, Victoria Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Basmadjian, Osvaldo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Fil: Baiardi, Gustavo Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Bregonzio Diaz, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Farmacología Experimental de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Farmacología Experimental de Córdoba; Argentina
Reunión conjunta; LXVIII Reunión anual de la Sociedad Argentina de Investigación Clínica; XXV Jornada anuales de la Sociedad Argentina de Biología; LV Reunión anual de la Asociación Argentina de Farmacología Experimental; VIII Reunión científica regional de la Asociación Argentina de Ciencia y Tecnología de Animlaes de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animlaes de Laboratorio
description Angiotensin II (Ang II), a pleiotropic neuropeptide, plays a critical role in regulating the sympathetic and neuroendocrine systems through the activation of AT 1 receptors (AT1-R). Studies on mammals have shown that administering Ang II through the intracerebroventricular (i.c.v) route increases water and sodium intake, as well as renal sodium excretion. Our group’s previous findings have shown that AT 1-R are involved in behavioural and neurochemical sensitization induced by amphetamine (Amph) administration. Our current aim is to assess the functional and neurochemical response to Ang II, via the AT 1-R activation, in animals that have been previously exposed to Amph. Male Wistar rats (250-320g) were injected intraperitoneally with Amph (2.5mg/kg/day) or saline for 5 days, and implanted with i.c.v. cannulae. Twenty-one days after the last Amph administration, the animals received Ang II (400pmol) i.c.v. First group: the animals were tested in a free choice paradigm for sodium (2% NaCl) and water intake, and sacrificed for Fos immunoreactivity determinations. Second group: urine and plasma samples were collected for electrolytes and plasma renin activity determination. Third group, the animals were tested in the plus maze or the holeboard for anxiety and memory work evaluation, respectively. Previous Amph exposure altered the physiological and behavioral responses described for central Ang II administration. Remarkably, the AT1-R blockade prevented most of these alterations but anxiety. Our results highlight that repeated Amph exposure attenuates the AT1-R functionality in a long-lasting manner, modifying the brain Ang II-induced responses.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/259269
Long-lasting amphetamine effects over cebtral Angiotensin II responses involve AT1-R; Reunión conjunta; LXVIII Reunión anual de la Sociedad Argentina de Investigación Clínica; XXV Jornada anuales de la Sociedad Argentina de Biología; LV Reunión anual de la Asociación Argentina de Farmacología Experimental; VIII Reunión científica regional de la Asociación Argentina de Ciencia y Tecnología de Animlaes de Laboratorio; Mar del Plata; Argentina; 2023; 159-159
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/259269
identifier_str_mv Long-lasting amphetamine effects over cebtral Angiotensin II responses involve AT1-R; Reunión conjunta; LXVIII Reunión anual de la Sociedad Argentina de Investigación Clínica; XXV Jornada anuales de la Sociedad Argentina de Biología; LV Reunión anual de la Asociación Argentina de Farmacología Experimental; VIII Reunión científica regional de la Asociación Argentina de Ciencia y Tecnología de Animlaes de Laboratorio; Mar del Plata; Argentina; 2023; 159-159
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.saic.org.ar/reuniones-anuales-previas
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundacion Revista Medicina
publisher.none.fl_str_mv Fundacion Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613709692928000
score 13.070432

Publicaciones similares