Highly variable AFLP and S-SAP markers for the identification of "Malbec" and "Syrah" clones

Autores
Stajner, N.; Jakse, J.; Javornik, B.; Masuelli, Ricardo Williams; Martínez, Liliana Estela
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The retrotransposon-based sequence-specific amplification polymorphism (S-SAP) and the amplified fragment length polymorphism (AFLP) marker systems were used to assess the clonal variation of 14 ‘Syrah’ and 22 ‘Malbec’ (Vitis vinifera L.) clones. The utility of S-SAP markers was compared to that of AFLP markers. On the basis of our results, S-SAP is more informative marker system and showed higher average number of polymorphic bands per cultivar group than AFLP. Relationships among clones were analyzed by cluster analysis using unweighted pair-groups using arithmetic averages and in both cases revealed well defined groups of clones, in which ‘Malbec’ clones were separated from ‘Syrah’ clones. High variability of some clones could also be seen within these clusters. The different levels of polymorphism for ‘Malbec’ and ‘Syrah’ obtained in this study suggest that ‘Malbec’ exhibits a higher mutation frequency than ‘Syrah’. Our results indicate that higher proportion of polymorphic bands in S-SAP makes it a less labour-intensive and more efficient approach for developing markers for clonal identification.
Fil: Stajner, N.. University of Ljubljana; Eslovenia
Fil: Jakse, J.. University of Ljubljana; Eslovenia
Fil: Javornik, B.. University of Ljubljana; Eslovenia
Fil: Masuelli, Ricardo Williams. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-san Juan. Estación Experimental Agropecuaria la Consulta. Agencia de Extensión Rural la Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Fil: Martínez, Liliana Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Materia
VITIS VINIFERA
RETROTRANSPOSON
CLONAL VARIATION
S-SAP AFLP
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/106686
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Highly variable AFLP and S-SAP markers for the identification of "Malbec" and "Syrah" clonesStajner, N.Jakse, J.Javornik, B.Masuelli, Ricardo WilliamsMartínez, Liliana EstelaVITIS VINIFERARETROTRANSPOSONCLONAL VARIATIONS-SAP AFLPhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The retrotransposon-based sequence-specific amplification polymorphism (S-SAP) and the amplified fragment length polymorphism (AFLP) marker systems were used to assess the clonal variation of 14 ‘Syrah’ and 22 ‘Malbec’ (Vitis vinifera L.) clones. The utility of S-SAP markers was compared to that of AFLP markers. On the basis of our results, S-SAP is more informative marker system and showed higher average number of polymorphic bands per cultivar group than AFLP. Relationships among clones were analyzed by cluster analysis using unweighted pair-groups using arithmetic averages and in both cases revealed well defined groups of clones, in which ‘Malbec’ clones were separated from ‘Syrah’ clones. High variability of some clones could also be seen within these clusters. The different levels of polymorphism for ‘Malbec’ and ‘Syrah’ obtained in this study suggest that ‘Malbec’ exhibits a higher mutation frequency than ‘Syrah’. Our results indicate that higher proportion of polymorphic bands in S-SAP makes it a less labour-intensive and more efficient approach for developing markers for clonal identification.Fil: Stajner, N.. University of Ljubljana; EsloveniaFil: Jakse, J.. University of Ljubljana; EsloveniaFil: Javornik, B.. University of Ljubljana; EsloveniaFil: Masuelli, Ricardo Williams. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-san Juan. Estación Experimental Agropecuaria la Consulta. Agencia de Extensión Rural la Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; ArgentinaFil: Martínez, Liliana Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaJKI-Institut für Rebenzüchtung2009-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/106686Stajner, N.; Jakse, J.; Javornik, B.; Masuelli, Ricardo Williams; Martínez, Liliana Estela; Highly variable AFLP and S-SAP markers for the identification of "Malbec" and "Syrah" clones; JKI-Institut für Rebenzüchtung; Vitis; 48; 3; 7-2009; 145-1500042-75002367-4156CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ojs.openagrar.de/index.php/VITIS/article/view/4159info:eu-repo/semantics/altIdentifier/doi/10.5073/vitis.2009.48.145-150info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:13:20Zoai:ri.conicet.gov.ar:11336/106686instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:13:20.676CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Highly variable AFLP and S-SAP markers for the identification of "Malbec" and "Syrah" clones
title Highly variable AFLP and S-SAP markers for the identification of "Malbec" and "Syrah" clones
spellingShingle Highly variable AFLP and S-SAP markers for the identification of "Malbec" and "Syrah" clones
Stajner, N.
VITIS VINIFERA
RETROTRANSPOSON
CLONAL VARIATION
S-SAP AFLP
title_short Highly variable AFLP and S-SAP markers for the identification of "Malbec" and "Syrah" clones
title_full Highly variable AFLP and S-SAP markers for the identification of "Malbec" and "Syrah" clones
title_fullStr Highly variable AFLP and S-SAP markers for the identification of "Malbec" and "Syrah" clones
title_full_unstemmed Highly variable AFLP and S-SAP markers for the identification of "Malbec" and "Syrah" clones
title_sort Highly variable AFLP and S-SAP markers for the identification of "Malbec" and "Syrah" clones
dc.creator.none.fl_str_mv Stajner, N.
Jakse, J.
Javornik, B.
Masuelli, Ricardo Williams
Martínez, Liliana Estela
author Stajner, N.
author_facet Stajner, N.
Jakse, J.
Javornik, B.
Masuelli, Ricardo Williams
Martínez, Liliana Estela
author_role author
author2 Jakse, J.
Javornik, B.
Masuelli, Ricardo Williams
Martínez, Liliana Estela
author2_role author
author
author
author
dc.subject.none.fl_str_mv VITIS VINIFERA
RETROTRANSPOSON
CLONAL VARIATION
S-SAP AFLP
topic VITIS VINIFERA
RETROTRANSPOSON
CLONAL VARIATION
S-SAP AFLP
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The retrotransposon-based sequence-specific amplification polymorphism (S-SAP) and the amplified fragment length polymorphism (AFLP) marker systems were used to assess the clonal variation of 14 ‘Syrah’ and 22 ‘Malbec’ (Vitis vinifera L.) clones. The utility of S-SAP markers was compared to that of AFLP markers. On the basis of our results, S-SAP is more informative marker system and showed higher average number of polymorphic bands per cultivar group than AFLP. Relationships among clones were analyzed by cluster analysis using unweighted pair-groups using arithmetic averages and in both cases revealed well defined groups of clones, in which ‘Malbec’ clones were separated from ‘Syrah’ clones. High variability of some clones could also be seen within these clusters. The different levels of polymorphism for ‘Malbec’ and ‘Syrah’ obtained in this study suggest that ‘Malbec’ exhibits a higher mutation frequency than ‘Syrah’. Our results indicate that higher proportion of polymorphic bands in S-SAP makes it a less labour-intensive and more efficient approach for developing markers for clonal identification.
Fil: Stajner, N.. University of Ljubljana; Eslovenia
Fil: Jakse, J.. University of Ljubljana; Eslovenia
Fil: Javornik, B.. University of Ljubljana; Eslovenia
Fil: Masuelli, Ricardo Williams. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-san Juan. Estación Experimental Agropecuaria la Consulta. Agencia de Extensión Rural la Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Fil: Martínez, Liliana Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
description The retrotransposon-based sequence-specific amplification polymorphism (S-SAP) and the amplified fragment length polymorphism (AFLP) marker systems were used to assess the clonal variation of 14 ‘Syrah’ and 22 ‘Malbec’ (Vitis vinifera L.) clones. The utility of S-SAP markers was compared to that of AFLP markers. On the basis of our results, S-SAP is more informative marker system and showed higher average number of polymorphic bands per cultivar group than AFLP. Relationships among clones were analyzed by cluster analysis using unweighted pair-groups using arithmetic averages and in both cases revealed well defined groups of clones, in which ‘Malbec’ clones were separated from ‘Syrah’ clones. High variability of some clones could also be seen within these clusters. The different levels of polymorphism for ‘Malbec’ and ‘Syrah’ obtained in this study suggest that ‘Malbec’ exhibits a higher mutation frequency than ‘Syrah’. Our results indicate that higher proportion of polymorphic bands in S-SAP makes it a less labour-intensive and more efficient approach for developing markers for clonal identification.
publishDate 2009
dc.date.none.fl_str_mv 2009-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/106686
Stajner, N.; Jakse, J.; Javornik, B.; Masuelli, Ricardo Williams; Martínez, Liliana Estela; Highly variable AFLP and S-SAP markers for the identification of "Malbec" and "Syrah" clones; JKI-Institut für Rebenzüchtung; Vitis; 48; 3; 7-2009; 145-150
0042-7500
2367-4156
CONICET Digital
CONICET
url http://hdl.handle.net/11336/106686
identifier_str_mv Stajner, N.; Jakse, J.; Javornik, B.; Masuelli, Ricardo Williams; Martínez, Liliana Estela; Highly variable AFLP and S-SAP markers for the identification of "Malbec" and "Syrah" clones; JKI-Institut für Rebenzüchtung; Vitis; 48; 3; 7-2009; 145-150
0042-7500
2367-4156
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ojs.openagrar.de/index.php/VITIS/article/view/4159
info:eu-repo/semantics/altIdentifier/doi/10.5073/vitis.2009.48.145-150
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv JKI-Institut für Rebenzüchtung
publisher.none.fl_str_mv JKI-Institut für Rebenzüchtung
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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