Use of Apple pomace as substrate for production of Lactiplantibacillus plantarum malolactic starter cultures

Autores
Cerdeira, Victoria; Brizuela, Natalia Soledad; Bravo Ferrada, Barbara Mercedes; Tymczyszyn, Emma Elizabeth
Año de publicación
2024
Idioma
inglés
Tipo de recurso
conjunto de datos
Estado
Descripción
The by-products of the food industry are an economic alternative as a source of nutrients to obtain biomass. At the same time, its use could solve the environmental problem related to their disposal, which is highly polluting due to their elevated biochemical oxygen demand. In this work we seek to optimize the production of cellular biomass of two native Patagonian strains of Lactiplantibacillus plantarum (UNQLp 11 and UNQLp155), selected for its oenological and technological properties, using apple pomace (AP), a residue from the juice and cider industry.The supplementation of APwith yeast extract, salts, and Tween 80 (sAP), proved to maintain the growth of the Lpb. plantarum strains, similar to the commercial medium used to grow LAB (De Man, Rogosa, Sharpe, MRS). Cultures grown in sAP medium showed good tolerance to wine conditions (high ethanol content and low pH), demonstrated by its ability to consume L-malic acid. The subsequent inoculation of these cultures in sterile wines (Merlot and Pinot noir) was carried out at laboratory scale, evaluating cell viability and L-malic acid consumption for 21 days at 21 ºC. Cultures grown in sAP media showed a similar performance to MRS media. Thus, sAP media proved to be a suitable substrate to grow oenological Lpb. plantarum strains(with high size inoculums) and cultures were able to drive malolactic fermentation, with an L-malic acid consumption higher than 90%.
Fil: Cerdeira, Victoria. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
Fil: Brizuela, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
Fil: Bravo Ferrada, Barbara Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
Fil: Tymczyszyn, Emma Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Protección de datos personales (Ley 25.326)
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/235907
Acceder
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spelling Use of Apple pomace as substrate for production of Lactiplantibacillus plantarum malolactic starter culturesCerdeira, VictoriaBrizuela, Natalia SoledadBravo Ferrada, Barbara MercedesTymczyszyn, Emma Elizabethhttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2The by-products of the food industry are an economic alternative as a source of nutrients to obtain biomass. At the same time, its use could solve the environmental problem related to their disposal, which is highly polluting due to their elevated biochemical oxygen demand. In this work we seek to optimize the production of cellular biomass of two native Patagonian strains of Lactiplantibacillus plantarum (UNQLp 11 and UNQLp155), selected for its oenological and technological properties, using apple pomace (AP), a residue from the juice and cider industry.The supplementation of APwith yeast extract, salts, and Tween 80 (sAP), proved to maintain the growth of the Lpb. plantarum strains, similar to the commercial medium used to grow LAB (De Man, Rogosa, Sharpe, MRS). Cultures grown in sAP medium showed good tolerance to wine conditions (high ethanol content and low pH), demonstrated by its ability to consume L-malic acid. The subsequent inoculation of these cultures in sterile wines (Merlot and Pinot noir) was carried out at laboratory scale, evaluating cell viability and L-malic acid consumption for 21 days at 21 ºC. Cultures grown in sAP media showed a similar performance to MRS media. Thus, sAP media proved to be a suitable substrate to grow oenological Lpb. plantarum strains(with high size inoculums) and cultures were able to drive malolactic fermentation, with an L-malic acid consumption higher than 90%.Fil: Cerdeira, Victoria. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; ArgentinaFil: Brizuela, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; ArgentinaFil: Bravo Ferrada, Barbara Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; ArgentinaFil: Tymczyszyn, Emma Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina2024info:ar-repo/semantics/conjuntoDeDatosv1.0info:eu-repo/semantics/dataSetapplication/octet-streamapplication/octet-streamapplication/octet-streamhttp://hdl.handle.net/11336/235907Cerdeira, Victoria; Brizuela, Natalia Soledad; Bravo Ferrada, Barbara Mercedes; Tymczyszyn, Emma Elizabeth; (2024): Use of Apple pomace as substrate for production of Lactiplantibacillus plantarum malolactic starter cultures. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/235907CONICET DigitalCONICETenginfo:eu-repo/grantAgreement/Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica/PICT 2019 Nº 0008info:eu-repo/semantics/restrictedAccessProtección de datos personales (Ley 25.326)reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:44Zoai:ri.conicet.gov.ar:11336/235907instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:44.579CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Use of Apple pomace as substrate for production of Lactiplantibacillus plantarum malolactic starter cultures
title Use of Apple pomace as substrate for production of Lactiplantibacillus plantarum malolactic starter cultures
spellingShingle Use of Apple pomace as substrate for production of Lactiplantibacillus plantarum malolactic starter cultures
Cerdeira, Victoria
title_short Use of Apple pomace as substrate for production of Lactiplantibacillus plantarum malolactic starter cultures
title_full Use of Apple pomace as substrate for production of Lactiplantibacillus plantarum malolactic starter cultures
title_fullStr Use of Apple pomace as substrate for production of Lactiplantibacillus plantarum malolactic starter cultures
title_full_unstemmed Use of Apple pomace as substrate for production of Lactiplantibacillus plantarum malolactic starter cultures
title_sort Use of Apple pomace as substrate for production of Lactiplantibacillus plantarum malolactic starter cultures
dc.creator.none.fl_str_mv Cerdeira, Victoria
Brizuela, Natalia Soledad
Bravo Ferrada, Barbara Mercedes
Tymczyszyn, Emma Elizabeth
author Cerdeira, Victoria
author_facet Cerdeira, Victoria
Brizuela, Natalia Soledad
Bravo Ferrada, Barbara Mercedes
Tymczyszyn, Emma Elizabeth
author_role author
author2 Brizuela, Natalia Soledad
Bravo Ferrada, Barbara Mercedes
Tymczyszyn, Emma Elizabeth
author2_role author
author
author
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The by-products of the food industry are an economic alternative as a source of nutrients to obtain biomass. At the same time, its use could solve the environmental problem related to their disposal, which is highly polluting due to their elevated biochemical oxygen demand. In this work we seek to optimize the production of cellular biomass of two native Patagonian strains of Lactiplantibacillus plantarum (UNQLp 11 and UNQLp155), selected for its oenological and technological properties, using apple pomace (AP), a residue from the juice and cider industry.The supplementation of APwith yeast extract, salts, and Tween 80 (sAP), proved to maintain the growth of the Lpb. plantarum strains, similar to the commercial medium used to grow LAB (De Man, Rogosa, Sharpe, MRS). Cultures grown in sAP medium showed good tolerance to wine conditions (high ethanol content and low pH), demonstrated by its ability to consume L-malic acid. The subsequent inoculation of these cultures in sterile wines (Merlot and Pinot noir) was carried out at laboratory scale, evaluating cell viability and L-malic acid consumption for 21 days at 21 ºC. Cultures grown in sAP media showed a similar performance to MRS media. Thus, sAP media proved to be a suitable substrate to grow oenological Lpb. plantarum strains(with high size inoculums) and cultures were able to drive malolactic fermentation, with an L-malic acid consumption higher than 90%.
Fil: Cerdeira, Victoria. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
Fil: Brizuela, Natalia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
Fil: Bravo Ferrada, Barbara Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
Fil: Tymczyszyn, Emma Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
description The by-products of the food industry are an economic alternative as a source of nutrients to obtain biomass. At the same time, its use could solve the environmental problem related to their disposal, which is highly polluting due to their elevated biochemical oxygen demand. In this work we seek to optimize the production of cellular biomass of two native Patagonian strains of Lactiplantibacillus plantarum (UNQLp 11 and UNQLp155), selected for its oenological and technological properties, using apple pomace (AP), a residue from the juice and cider industry.The supplementation of APwith yeast extract, salts, and Tween 80 (sAP), proved to maintain the growth of the Lpb. plantarum strains, similar to the commercial medium used to grow LAB (De Man, Rogosa, Sharpe, MRS). Cultures grown in sAP medium showed good tolerance to wine conditions (high ethanol content and low pH), demonstrated by its ability to consume L-malic acid. The subsequent inoculation of these cultures in sterile wines (Merlot and Pinot noir) was carried out at laboratory scale, evaluating cell viability and L-malic acid consumption for 21 days at 21 ºC. Cultures grown in sAP media showed a similar performance to MRS media. Thus, sAP media proved to be a suitable substrate to grow oenological Lpb. plantarum strains(with high size inoculums) and cultures were able to drive malolactic fermentation, with an L-malic acid consumption higher than 90%.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:ar-repo/semantics/conjuntoDeDatos
v1.0
info:eu-repo/semantics/dataSet
format dataSet
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/235907
Cerdeira, Victoria; Brizuela, Natalia Soledad; Bravo Ferrada, Barbara Mercedes; Tymczyszyn, Emma Elizabeth; (2024): Use of Apple pomace as substrate for production of Lactiplantibacillus plantarum malolactic starter cultures. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/235907
CONICET Digital
CONICET
url http://hdl.handle.net/11336/235907
identifier_str_mv Cerdeira, Victoria; Brizuela, Natalia Soledad; Bravo Ferrada, Barbara Mercedes; Tymczyszyn, Emma Elizabeth; (2024): Use of Apple pomace as substrate for production of Lactiplantibacillus plantarum malolactic starter cultures. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/235907
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/Ministerio de Ciencia. Tecnología e Innovación Productiva. Agencia Nacional de Promoción Científica y Tecnológica/PICT 2019 Nº 0008
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
Protección de datos personales (Ley 25.326)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv Protección de datos personales (Ley 25.326)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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