An algal-derived DL-galactan hybrid is an efficient preventing agent for in vitro dengue virus infection

Autores
Talarico, Laura Beatriz; Duarte, María E. R.; Zibetti, Rosiane G. M.; Noseda, Miguel D.; Damonte, Elsa Beatriz
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The DL-galactan hybrid C2S-3, isolated from the red seaweed Cryptonemia crenulata (Halymeniaceae, Halymeniales), is a potent and selective inhibitor of the multiplication of diverse strains of DENV-2 in Vero cells with higher effectiveness than the reference polysaccharide heparin. The presence of the compound either only at virus adsorption or at virus internalization exerted a significant and dose-dependent inhibition in DENV-2 plaque number. The compound failed to inactivate DENV-2 directly by incubation of virus before cell infection as well as to induce a refractory state by cell pretreatment. Thus, the inhibitory effect was exclusively exerted through a blockade in virus multiplication during the infectious process. When the entry of DENV-2 particles into the cell is bypassed, as occurs in virus RNA transfection, the polysaccharide C2S-3 failed to block the completion of the multiplication cycle. Furthermore, the antiviral properties of C2S-3 are not correlated with anticoagulant activity.
Fil: Talarico, Laura Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Duarte, María E. R.. Universidade Federal do Paraná; Brasil
Fil: Zibetti, Rosiane G. M.. Universidade Federal do Paraná; Brasil. Instituto de Ensino Superior Pequeno Príncipe; Brasil
Fil: Noseda, Miguel D.. Universidade Federal do Paraná; Brasil
Fil: Damonte, Elsa Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Materia
Antiviral Agent
Cryptonemia Crenulata
Dengue Virus
Halymeniaceae
Marine Alga
Sulfated Galactan
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/83862
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/83862
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An algal-derived DL-galactan hybrid is an efficient preventing agent for in vitro dengue virus infectionTalarico, Laura BeatrizDuarte, María E. R.Zibetti, Rosiane G. M.Noseda, Miguel D.Damonte, Elsa BeatrizAntiviral AgentCryptonemia CrenulataDengue VirusHalymeniaceaeMarine AlgaSulfated Galactanhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The DL-galactan hybrid C2S-3, isolated from the red seaweed Cryptonemia crenulata (Halymeniaceae, Halymeniales), is a potent and selective inhibitor of the multiplication of diverse strains of DENV-2 in Vero cells with higher effectiveness than the reference polysaccharide heparin. The presence of the compound either only at virus adsorption or at virus internalization exerted a significant and dose-dependent inhibition in DENV-2 plaque number. The compound failed to inactivate DENV-2 directly by incubation of virus before cell infection as well as to induce a refractory state by cell pretreatment. Thus, the inhibitory effect was exclusively exerted through a blockade in virus multiplication during the infectious process. When the entry of DENV-2 particles into the cell is bypassed, as occurs in virus RNA transfection, the polysaccharide C2S-3 failed to block the completion of the multiplication cycle. Furthermore, the antiviral properties of C2S-3 are not correlated with anticoagulant activity.Fil: Talarico, Laura Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Duarte, María E. R.. Universidade Federal do Paraná; BrasilFil: Zibetti, Rosiane G. M.. Universidade Federal do Paraná; Brasil. Instituto de Ensino Superior Pequeno Príncipe; BrasilFil: Noseda, Miguel D.. Universidade Federal do Paraná; BrasilFil: Damonte, Elsa Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaGeorg Thieme Verlag Kg2007-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/83862Talarico, Laura Beatriz; Duarte, María E. R.; Zibetti, Rosiane G. M.; Noseda, Miguel D.; Damonte, Elsa Beatriz; An algal-derived DL-galactan hybrid is an efficient preventing agent for in vitro dengue virus infection; Georg Thieme Verlag Kg; Planta Medica; 73; 14; 11-2007; 1464-14680032-0943CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.thieme-connect.de/products/ejournals/abstract/10.1055/s-2007-990241info:eu-repo/semantics/altIdentifier/doi/10.1055/s-2007-990241info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:10:59Zoai:ri.conicet.gov.ar:11336/83862instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:11:00.114CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An algal-derived DL-galactan hybrid is an efficient preventing agent for in vitro dengue virus infection
title An algal-derived DL-galactan hybrid is an efficient preventing agent for in vitro dengue virus infection
spellingShingle An algal-derived DL-galactan hybrid is an efficient preventing agent for in vitro dengue virus infection
Talarico, Laura Beatriz
Antiviral Agent
Cryptonemia Crenulata
Dengue Virus
Halymeniaceae
Marine Alga
Sulfated Galactan
title_short An algal-derived DL-galactan hybrid is an efficient preventing agent for in vitro dengue virus infection
title_full An algal-derived DL-galactan hybrid is an efficient preventing agent for in vitro dengue virus infection
title_fullStr An algal-derived DL-galactan hybrid is an efficient preventing agent for in vitro dengue virus infection
title_full_unstemmed An algal-derived DL-galactan hybrid is an efficient preventing agent for in vitro dengue virus infection
title_sort An algal-derived DL-galactan hybrid is an efficient preventing agent for in vitro dengue virus infection
dc.creator.none.fl_str_mv Talarico, Laura Beatriz
Duarte, María E. R.
Zibetti, Rosiane G. M.
Noseda, Miguel D.
Damonte, Elsa Beatriz
author Talarico, Laura Beatriz
author_facet Talarico, Laura Beatriz
Duarte, María E. R.
Zibetti, Rosiane G. M.
Noseda, Miguel D.
Damonte, Elsa Beatriz
author_role author
author2 Duarte, María E. R.
Zibetti, Rosiane G. M.
Noseda, Miguel D.
Damonte, Elsa Beatriz
author2_role author
author
author
author
dc.subject.none.fl_str_mv Antiviral Agent
Cryptonemia Crenulata
Dengue Virus
Halymeniaceae
Marine Alga
Sulfated Galactan
topic Antiviral Agent
Cryptonemia Crenulata
Dengue Virus
Halymeniaceae
Marine Alga
Sulfated Galactan
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The DL-galactan hybrid C2S-3, isolated from the red seaweed Cryptonemia crenulata (Halymeniaceae, Halymeniales), is a potent and selective inhibitor of the multiplication of diverse strains of DENV-2 in Vero cells with higher effectiveness than the reference polysaccharide heparin. The presence of the compound either only at virus adsorption or at virus internalization exerted a significant and dose-dependent inhibition in DENV-2 plaque number. The compound failed to inactivate DENV-2 directly by incubation of virus before cell infection as well as to induce a refractory state by cell pretreatment. Thus, the inhibitory effect was exclusively exerted through a blockade in virus multiplication during the infectious process. When the entry of DENV-2 particles into the cell is bypassed, as occurs in virus RNA transfection, the polysaccharide C2S-3 failed to block the completion of the multiplication cycle. Furthermore, the antiviral properties of C2S-3 are not correlated with anticoagulant activity.
Fil: Talarico, Laura Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Duarte, María E. R.. Universidade Federal do Paraná; Brasil
Fil: Zibetti, Rosiane G. M.. Universidade Federal do Paraná; Brasil. Instituto de Ensino Superior Pequeno Príncipe; Brasil
Fil: Noseda, Miguel D.. Universidade Federal do Paraná; Brasil
Fil: Damonte, Elsa Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
description The DL-galactan hybrid C2S-3, isolated from the red seaweed Cryptonemia crenulata (Halymeniaceae, Halymeniales), is a potent and selective inhibitor of the multiplication of diverse strains of DENV-2 in Vero cells with higher effectiveness than the reference polysaccharide heparin. The presence of the compound either only at virus adsorption or at virus internalization exerted a significant and dose-dependent inhibition in DENV-2 plaque number. The compound failed to inactivate DENV-2 directly by incubation of virus before cell infection as well as to induce a refractory state by cell pretreatment. Thus, the inhibitory effect was exclusively exerted through a blockade in virus multiplication during the infectious process. When the entry of DENV-2 particles into the cell is bypassed, as occurs in virus RNA transfection, the polysaccharide C2S-3 failed to block the completion of the multiplication cycle. Furthermore, the antiviral properties of C2S-3 are not correlated with anticoagulant activity.
publishDate 2007
dc.date.none.fl_str_mv 2007-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/83862
Talarico, Laura Beatriz; Duarte, María E. R.; Zibetti, Rosiane G. M.; Noseda, Miguel D.; Damonte, Elsa Beatriz; An algal-derived DL-galactan hybrid is an efficient preventing agent for in vitro dengue virus infection; Georg Thieme Verlag Kg; Planta Medica; 73; 14; 11-2007; 1464-1468
0032-0943
CONICET Digital
CONICET
url http://hdl.handle.net/11336/83862
identifier_str_mv Talarico, Laura Beatriz; Duarte, María E. R.; Zibetti, Rosiane G. M.; Noseda, Miguel D.; Damonte, Elsa Beatriz; An algal-derived DL-galactan hybrid is an efficient preventing agent for in vitro dengue virus infection; Georg Thieme Verlag Kg; Planta Medica; 73; 14; 11-2007; 1464-1468
0032-0943
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.thieme-connect.de/products/ejournals/abstract/10.1055/s-2007-990241
info:eu-repo/semantics/altIdentifier/doi/10.1055/s-2007-990241
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Georg Thieme Verlag Kg
publisher.none.fl_str_mv Georg Thieme Verlag Kg
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781479397359616
score 12.982451

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