Quorum and light signals modulate acetoin/Butanediol catabolism in acinetobacterspp

Autores
Tuttobene, Marisel Romina; Fernández García, Laura; Blasco, Lucía; Cribb, Pamela; Ambroa, Anton; Müller, Gabriela Leticia; Fernández Cuenca, Felipe; Bleriot, Inés; Rodríguez, Ramiro Esteban; Barbosa, Beatriz G. V.; Lopez Rojas, Rafael; Trastoy, Rocío; López, María; Bou, Germán; Tomás, María; Mussi, María Alejandra
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Acinetobacter spp. are found in all environments on Earth due to their extraordinary capacity to survive in the presence of physical and chemical stressors. In this study, we analyzed global gene expression in airborne Acinetobacter sp. strain 5-2Ac02 isolated from hospital environment in response to quorum network modulators and found that they induced the expression of genes of the acetoin/butanediol catabolism, volatile compounds shown to mediate interkingdom interactions. Interestingly, the acoN gene, annotated as a putative transcriptional regulator, was truncated in the downstream regulatory region of the induced acetoin/butanediol cluster in Acinetobacter sp. strain 5-2Ac02, and its functioning as a negative regulator of this cluster integrating quorum signals was confirmed in Acinetobacter baumannii ATCC 17978. Moreover, we show that the acetoin catabolism is also induced by light and provide insights into the light transduction mechanism by showing that the photoreceptor BlsA interacts with and antagonizes the functioning of AcoN in A. baumannii, integrating also a temperature signal. The data support a model in which BlsA interacts with and likely sequesters AcoN at this condition, relieving acetoin catabolic genes from repression, and leading to better growth under blue light. This photoregulation depends on temperature, occurring at 23°C but not at 30°C. BlsA is thus a dual regulator, modulating different transcriptional regulators in the dark but also under blue light, representing thus a novel concept. The overall data show that quorum modulators as well as light regulate the acetoin catabolic cluster, providing a better understanding of environmental as well as clinical bacteria.
Fil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Fernández García, Laura. Universidad da Coruña; España
Fil: Blasco, Lucía. Universidad da Coruña; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cribb, Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Ambroa, Anton. Universidad da Coruña; España
Fil: Müller, Gabriela Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Fernández Cuenca, Felipe. Consejo Superior de Investigaciones Científicas; España
Fil: Bleriot, Inés. Universidad de Sevilla; España
Fil: Rodríguez, Ramiro Esteban. Hospital Universitario Virgen Macarena; España
Fil: Barbosa, Beatriz G. V.. Universidade Federal de Pernambuco; Brasil
Fil: Lopez Rojas, Rafael. Universidad de Sevilla; España
Fil: Trastoy, Rocío. Universidad da Coruña; España
Fil: López, María. Universidad da Coruña; España
Fil: Bou, Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Tomás, María. Universidad da Coruña; España
Fil: Mussi, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Materia
ACETOIN
ACINETOBACTER
ACON
BLSA
LIGHT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/183865
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/183865
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Quorum and light signals modulate acetoin/Butanediol catabolism in acinetobactersppTuttobene, Marisel RominaFernández García, LauraBlasco, LucíaCribb, PamelaAmbroa, AntonMüller, Gabriela LeticiaFernández Cuenca, FelipeBleriot, InésRodríguez, Ramiro EstebanBarbosa, Beatriz G. V.Lopez Rojas, RafaelTrastoy, RocíoLópez, MaríaBou, GermánTomás, MaríaMussi, María AlejandraACETOINACINETOBACTERACONBLSALIGHThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Acinetobacter spp. are found in all environments on Earth due to their extraordinary capacity to survive in the presence of physical and chemical stressors. In this study, we analyzed global gene expression in airborne Acinetobacter sp. strain 5-2Ac02 isolated from hospital environment in response to quorum network modulators and found that they induced the expression of genes of the acetoin/butanediol catabolism, volatile compounds shown to mediate interkingdom interactions. Interestingly, the acoN gene, annotated as a putative transcriptional regulator, was truncated in the downstream regulatory region of the induced acetoin/butanediol cluster in Acinetobacter sp. strain 5-2Ac02, and its functioning as a negative regulator of this cluster integrating quorum signals was confirmed in Acinetobacter baumannii ATCC 17978. Moreover, we show that the acetoin catabolism is also induced by light and provide insights into the light transduction mechanism by showing that the photoreceptor BlsA interacts with and antagonizes the functioning of AcoN in A. baumannii, integrating also a temperature signal. The data support a model in which BlsA interacts with and likely sequesters AcoN at this condition, relieving acetoin catabolic genes from repression, and leading to better growth under blue light. This photoregulation depends on temperature, occurring at 23°C but not at 30°C. BlsA is thus a dual regulator, modulating different transcriptional regulators in the dark but also under blue light, representing thus a novel concept. The overall data show that quorum modulators as well as light regulate the acetoin catabolic cluster, providing a better understanding of environmental as well as clinical bacteria.Fil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Fernández García, Laura. Universidad da Coruña; EspañaFil: Blasco, Lucía. Universidad da Coruña; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cribb, Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Ambroa, Anton. Universidad da Coruña; EspañaFil: Müller, Gabriela Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Fernández Cuenca, Felipe. Consejo Superior de Investigaciones Científicas; EspañaFil: Bleriot, Inés. Universidad de Sevilla; EspañaFil: Rodríguez, Ramiro Esteban. Hospital Universitario Virgen Macarena; EspañaFil: Barbosa, Beatriz G. V.. Universidade Federal de Pernambuco; BrasilFil: Lopez Rojas, Rafael. Universidad de Sevilla; EspañaFil: Trastoy, Rocío. Universidad da Coruña; EspañaFil: López, María. Universidad da Coruña; EspañaFil: Bou, Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Tomás, María. Universidad da Coruña; EspañaFil: Mussi, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFrontiers Media2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/183865Tuttobene, Marisel Romina; Fernández García, Laura; Blasco, Lucía; Cribb, Pamela; Ambroa, Anton; et al.; Quorum and light signals modulate acetoin/Butanediol catabolism in acinetobacterspp; Frontiers Media; Frontiers in Microbiology; 10; 6-2019; 1-171664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2019.01376/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.01376info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:34Zoai:ri.conicet.gov.ar:11336/183865instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:35.237CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Quorum and light signals modulate acetoin/Butanediol catabolism in acinetobacterspp
title Quorum and light signals modulate acetoin/Butanediol catabolism in acinetobacterspp
spellingShingle Quorum and light signals modulate acetoin/Butanediol catabolism in acinetobacterspp
Tuttobene, Marisel Romina
ACETOIN
ACINETOBACTER
ACON
BLSA
LIGHT
title_short Quorum and light signals modulate acetoin/Butanediol catabolism in acinetobacterspp
title_full Quorum and light signals modulate acetoin/Butanediol catabolism in acinetobacterspp
title_fullStr Quorum and light signals modulate acetoin/Butanediol catabolism in acinetobacterspp
title_full_unstemmed Quorum and light signals modulate acetoin/Butanediol catabolism in acinetobacterspp
title_sort Quorum and light signals modulate acetoin/Butanediol catabolism in acinetobacterspp
dc.creator.none.fl_str_mv Tuttobene, Marisel Romina
Fernández García, Laura
Blasco, Lucía
Cribb, Pamela
Ambroa, Anton
Müller, Gabriela Leticia
Fernández Cuenca, Felipe
Bleriot, Inés
Rodríguez, Ramiro Esteban
Barbosa, Beatriz G. V.
Lopez Rojas, Rafael
Trastoy, Rocío
López, María
Bou, Germán
Tomás, María
Mussi, María Alejandra
author Tuttobene, Marisel Romina
author_facet Tuttobene, Marisel Romina
Fernández García, Laura
Blasco, Lucía
Cribb, Pamela
Ambroa, Anton
Müller, Gabriela Leticia
Fernández Cuenca, Felipe
Bleriot, Inés
Rodríguez, Ramiro Esteban
Barbosa, Beatriz G. V.
Lopez Rojas, Rafael
Trastoy, Rocío
López, María
Bou, Germán
Tomás, María
Mussi, María Alejandra
author_role author
author2 Fernández García, Laura
Blasco, Lucía
Cribb, Pamela
Ambroa, Anton
Müller, Gabriela Leticia
Fernández Cuenca, Felipe
Bleriot, Inés
Rodríguez, Ramiro Esteban
Barbosa, Beatriz G. V.
Lopez Rojas, Rafael
Trastoy, Rocío
López, María
Bou, Germán
Tomás, María
Mussi, María Alejandra
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACETOIN
ACINETOBACTER
ACON
BLSA
LIGHT
topic ACETOIN
ACINETOBACTER
ACON
BLSA
LIGHT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Acinetobacter spp. are found in all environments on Earth due to their extraordinary capacity to survive in the presence of physical and chemical stressors. In this study, we analyzed global gene expression in airborne Acinetobacter sp. strain 5-2Ac02 isolated from hospital environment in response to quorum network modulators and found that they induced the expression of genes of the acetoin/butanediol catabolism, volatile compounds shown to mediate interkingdom interactions. Interestingly, the acoN gene, annotated as a putative transcriptional regulator, was truncated in the downstream regulatory region of the induced acetoin/butanediol cluster in Acinetobacter sp. strain 5-2Ac02, and its functioning as a negative regulator of this cluster integrating quorum signals was confirmed in Acinetobacter baumannii ATCC 17978. Moreover, we show that the acetoin catabolism is also induced by light and provide insights into the light transduction mechanism by showing that the photoreceptor BlsA interacts with and antagonizes the functioning of AcoN in A. baumannii, integrating also a temperature signal. The data support a model in which BlsA interacts with and likely sequesters AcoN at this condition, relieving acetoin catabolic genes from repression, and leading to better growth under blue light. This photoregulation depends on temperature, occurring at 23°C but not at 30°C. BlsA is thus a dual regulator, modulating different transcriptional regulators in the dark but also under blue light, representing thus a novel concept. The overall data show that quorum modulators as well as light regulate the acetoin catabolic cluster, providing a better understanding of environmental as well as clinical bacteria.
Fil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Fernández García, Laura. Universidad da Coruña; España
Fil: Blasco, Lucía. Universidad da Coruña; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cribb, Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Ambroa, Anton. Universidad da Coruña; España
Fil: Müller, Gabriela Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Fernández Cuenca, Felipe. Consejo Superior de Investigaciones Científicas; España
Fil: Bleriot, Inés. Universidad de Sevilla; España
Fil: Rodríguez, Ramiro Esteban. Hospital Universitario Virgen Macarena; España
Fil: Barbosa, Beatriz G. V.. Universidade Federal de Pernambuco; Brasil
Fil: Lopez Rojas, Rafael. Universidad de Sevilla; España
Fil: Trastoy, Rocío. Universidad da Coruña; España
Fil: López, María. Universidad da Coruña; España
Fil: Bou, Germán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Tomás, María. Universidad da Coruña; España
Fil: Mussi, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
description Acinetobacter spp. are found in all environments on Earth due to their extraordinary capacity to survive in the presence of physical and chemical stressors. In this study, we analyzed global gene expression in airborne Acinetobacter sp. strain 5-2Ac02 isolated from hospital environment in response to quorum network modulators and found that they induced the expression of genes of the acetoin/butanediol catabolism, volatile compounds shown to mediate interkingdom interactions. Interestingly, the acoN gene, annotated as a putative transcriptional regulator, was truncated in the downstream regulatory region of the induced acetoin/butanediol cluster in Acinetobacter sp. strain 5-2Ac02, and its functioning as a negative regulator of this cluster integrating quorum signals was confirmed in Acinetobacter baumannii ATCC 17978. Moreover, we show that the acetoin catabolism is also induced by light and provide insights into the light transduction mechanism by showing that the photoreceptor BlsA interacts with and antagonizes the functioning of AcoN in A. baumannii, integrating also a temperature signal. The data support a model in which BlsA interacts with and likely sequesters AcoN at this condition, relieving acetoin catabolic genes from repression, and leading to better growth under blue light. This photoregulation depends on temperature, occurring at 23°C but not at 30°C. BlsA is thus a dual regulator, modulating different transcriptional regulators in the dark but also under blue light, representing thus a novel concept. The overall data show that quorum modulators as well as light regulate the acetoin catabolic cluster, providing a better understanding of environmental as well as clinical bacteria.
publishDate 2019
dc.date.none.fl_str_mv 2019-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/183865
Tuttobene, Marisel Romina; Fernández García, Laura; Blasco, Lucía; Cribb, Pamela; Ambroa, Anton; et al.; Quorum and light signals modulate acetoin/Butanediol catabolism in acinetobacterspp; Frontiers Media; Frontiers in Microbiology; 10; 6-2019; 1-17
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/183865
identifier_str_mv Tuttobene, Marisel Romina; Fernández García, Laura; Blasco, Lucía; Cribb, Pamela; Ambroa, Anton; et al.; Quorum and light signals modulate acetoin/Butanediol catabolism in acinetobacterspp; Frontiers Media; Frontiers in Microbiology; 10; 6-2019; 1-17
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2019.01376/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.01376
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.11938

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