Specific immune response induced by a lactobacillus associated with a pneumococcal antigen in an "in vitro" human cells model

Autores
Vintiñi, Elisa Ofelia; Gonzalez, Laura; Medina, Marcela Susana
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work we assessed the immune response induced by combinations of live (LcV) and heat-killed (LcM)Lactobacillus casei CRL 431 as adjuvants associated with pneumococcal protective A protein (PppA) in peripheral blood mononuclear cells (PBMCs). LcV, LcM and their combinations with PppA stimulated T, B and NK cells. Thus, all stimuli increased CD25 expression in T CD3 lymphocytes, highest activation being reached with the combinations of LcV or LcM with an antigen (PppA+LcV, PppA+LcM). Expression of CD19 B cells marker was significantly increased in almost all treatments compared with non-stimulated PBMCs, except for PppA. All treatments increased CD86 expression in the LT population, while in B cells only LPS, PppA+LcV and PppA+LcM increased it. NK cells were significantly increased by LPS (P<0.05), PppA+LcM (P<0.01) and PppA+LcV (P<0.01) compared to non-stimulated PBMCs. PppA+LcV and PppA+LcM increased CD56 expression in both NKT and NK cells, while LcM expanded NKT population. Cytokine pattern analysis showed that LcV and LcM stimulated Th, Th2 and Th17 cytokines and exerted an important adjuvant effect when associated with PppA. Correlation with previous results obtained in animal models when the same experimental vaccine was nasally administered is discussed. Human PBMCs would be useful to evaluate the immune response of mucosal vaccines containing lactic acid bacteria associated with a specific antigen.
Fil: Vintiñi, Elisa Ofelia. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Gonzalez, Laura. Universidad Nacional de Tucuman. Facultad de Medicina; Argentina
Fil: Medina, Marcela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Materia
PBMCs
VACCINE
HEAT-KILLED LACTOBACILLI
PNEUMOCOCCAL ANTIGEN
IMMUNE RESPONSE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25288
Acceder
id CONICETDig_bce50a0c214febb0421ba932c2a89848
oai_identifier_str oai:ri.conicet.gov.ar:11336/25288
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Specific immune response induced by a lactobacillus associated with a pneumococcal antigen in an "in vitro" human cells modelVintiñi, Elisa OfeliaGonzalez, LauraMedina, Marcela SusanaPBMCsVACCINEHEAT-KILLED LACTOBACILLIPNEUMOCOCCAL ANTIGENIMMUNE RESPONSEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In this work we assessed the immune response induced by combinations of live (LcV) and heat-killed (LcM)Lactobacillus casei CRL 431 as adjuvants associated with pneumococcal protective A protein (PppA) in peripheral blood mononuclear cells (PBMCs). LcV, LcM and their combinations with PppA stimulated T, B and NK cells. Thus, all stimuli increased CD25 expression in T CD3 lymphocytes, highest activation being reached with the combinations of LcV or LcM with an antigen (PppA+LcV, PppA+LcM). Expression of CD19 B cells marker was significantly increased in almost all treatments compared with non-stimulated PBMCs, except for PppA. All treatments increased CD86 expression in the LT population, while in B cells only LPS, PppA+LcV and PppA+LcM increased it. NK cells were significantly increased by LPS (P<0.05), PppA+LcM (P<0.01) and PppA+LcV (P<0.01) compared to non-stimulated PBMCs. PppA+LcV and PppA+LcM increased CD56 expression in both NKT and NK cells, while LcM expanded NKT population. Cytokine pattern analysis showed that LcV and LcM stimulated Th, Th2 and Th17 cytokines and exerted an important adjuvant effect when associated with PppA. Correlation with previous results obtained in animal models when the same experimental vaccine was nasally administered is discussed. Human PBMCs would be useful to evaluate the immune response of mucosal vaccines containing lactic acid bacteria associated with a specific antigen.Fil: Vintiñi, Elisa Ofelia. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; ArgentinaFil: Gonzalez, Laura. Universidad Nacional de Tucuman. Facultad de Medicina; ArgentinaFil: Medina, Marcela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaOMICS Publishing Group2012-10-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25288Vintiñi, Elisa Ofelia; Gonzalez, Laura; Medina, Marcela Susana; Specific immune response induced by a lactobacillus associated with a pneumococcal antigen in an "in vitro" human cells model; OMICS Publishing Group; Vaccines & Vaccination; 3; 6; 16-10-2012; 1-7; 1000153-10001532157-75602157-7560CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.4172/2157-7560.1000153info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/specific-immune-response-induced-by-a-lactobacillus-associated-with-a-pneumococcal-antigen-in-an-in-vitro-human-cells-model-2157-7560.1000153.php?aid=9058info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T12:16:55Zoai:ri.conicet.gov.ar:11336/25288instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 12:16:55.687CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Specific immune response induced by a lactobacillus associated with a pneumococcal antigen in an "in vitro" human cells model
title Specific immune response induced by a lactobacillus associated with a pneumococcal antigen in an "in vitro" human cells model
spellingShingle Specific immune response induced by a lactobacillus associated with a pneumococcal antigen in an "in vitro" human cells model
Vintiñi, Elisa Ofelia
PBMCs
VACCINE
HEAT-KILLED LACTOBACILLI
PNEUMOCOCCAL ANTIGEN
IMMUNE RESPONSE
title_short Specific immune response induced by a lactobacillus associated with a pneumococcal antigen in an "in vitro" human cells model
title_full Specific immune response induced by a lactobacillus associated with a pneumococcal antigen in an "in vitro" human cells model
title_fullStr Specific immune response induced by a lactobacillus associated with a pneumococcal antigen in an "in vitro" human cells model
title_full_unstemmed Specific immune response induced by a lactobacillus associated with a pneumococcal antigen in an "in vitro" human cells model
title_sort Specific immune response induced by a lactobacillus associated with a pneumococcal antigen in an "in vitro" human cells model
dc.creator.none.fl_str_mv Vintiñi, Elisa Ofelia
Gonzalez, Laura
Medina, Marcela Susana
author Vintiñi, Elisa Ofelia
author_facet Vintiñi, Elisa Ofelia
Gonzalez, Laura
Medina, Marcela Susana
author_role author
author2 Gonzalez, Laura
Medina, Marcela Susana
author2_role author
author
dc.subject.none.fl_str_mv PBMCs
VACCINE
HEAT-KILLED LACTOBACILLI
PNEUMOCOCCAL ANTIGEN
IMMUNE RESPONSE
topic PBMCs
VACCINE
HEAT-KILLED LACTOBACILLI
PNEUMOCOCCAL ANTIGEN
IMMUNE RESPONSE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this work we assessed the immune response induced by combinations of live (LcV) and heat-killed (LcM)Lactobacillus casei CRL 431 as adjuvants associated with pneumococcal protective A protein (PppA) in peripheral blood mononuclear cells (PBMCs). LcV, LcM and their combinations with PppA stimulated T, B and NK cells. Thus, all stimuli increased CD25 expression in T CD3 lymphocytes, highest activation being reached with the combinations of LcV or LcM with an antigen (PppA+LcV, PppA+LcM). Expression of CD19 B cells marker was significantly increased in almost all treatments compared with non-stimulated PBMCs, except for PppA. All treatments increased CD86 expression in the LT population, while in B cells only LPS, PppA+LcV and PppA+LcM increased it. NK cells were significantly increased by LPS (P<0.05), PppA+LcM (P<0.01) and PppA+LcV (P<0.01) compared to non-stimulated PBMCs. PppA+LcV and PppA+LcM increased CD56 expression in both NKT and NK cells, while LcM expanded NKT population. Cytokine pattern analysis showed that LcV and LcM stimulated Th, Th2 and Th17 cytokines and exerted an important adjuvant effect when associated with PppA. Correlation with previous results obtained in animal models when the same experimental vaccine was nasally administered is discussed. Human PBMCs would be useful to evaluate the immune response of mucosal vaccines containing lactic acid bacteria associated with a specific antigen.
Fil: Vintiñi, Elisa Ofelia. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina
Fil: Gonzalez, Laura. Universidad Nacional de Tucuman. Facultad de Medicina; Argentina
Fil: Medina, Marcela Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
description In this work we assessed the immune response induced by combinations of live (LcV) and heat-killed (LcM)Lactobacillus casei CRL 431 as adjuvants associated with pneumococcal protective A protein (PppA) in peripheral blood mononuclear cells (PBMCs). LcV, LcM and their combinations with PppA stimulated T, B and NK cells. Thus, all stimuli increased CD25 expression in T CD3 lymphocytes, highest activation being reached with the combinations of LcV or LcM with an antigen (PppA+LcV, PppA+LcM). Expression of CD19 B cells marker was significantly increased in almost all treatments compared with non-stimulated PBMCs, except for PppA. All treatments increased CD86 expression in the LT population, while in B cells only LPS, PppA+LcV and PppA+LcM increased it. NK cells were significantly increased by LPS (P<0.05), PppA+LcM (P<0.01) and PppA+LcV (P<0.01) compared to non-stimulated PBMCs. PppA+LcV and PppA+LcM increased CD56 expression in both NKT and NK cells, while LcM expanded NKT population. Cytokine pattern analysis showed that LcV and LcM stimulated Th, Th2 and Th17 cytokines and exerted an important adjuvant effect when associated with PppA. Correlation with previous results obtained in animal models when the same experimental vaccine was nasally administered is discussed. Human PBMCs would be useful to evaluate the immune response of mucosal vaccines containing lactic acid bacteria associated with a specific antigen.
publishDate 2012
dc.date.none.fl_str_mv 2012-10-16
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25288
Vintiñi, Elisa Ofelia; Gonzalez, Laura; Medina, Marcela Susana; Specific immune response induced by a lactobacillus associated with a pneumococcal antigen in an "in vitro" human cells model; OMICS Publishing Group; Vaccines & Vaccination; 3; 6; 16-10-2012; 1-7; 1000153-1000153
2157-7560
2157-7560
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25288
identifier_str_mv Vintiñi, Elisa Ofelia; Gonzalez, Laura; Medina, Marcela Susana; Specific immune response induced by a lactobacillus associated with a pneumococcal antigen in an "in vitro" human cells model; OMICS Publishing Group; Vaccines & Vaccination; 3; 6; 16-10-2012; 1-7; 1000153-1000153
2157-7560
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.4172/2157-7560.1000153
info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/specific-immune-response-induced-by-a-lactobacillus-associated-with-a-pneumococcal-antigen-in-an-in-vitro-human-cells-model-2157-7560.1000153.php?aid=9058
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv OMICS Publishing Group
publisher.none.fl_str_mv OMICS Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.10058

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