Catalytic activity of a novel serine/threonine protein phosphatase PP5 from Leishmania major

Autores
Norris Mullins, Brianna; Vacchina, Paola; Morales, Miguel A.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Leishmaniasis is a vector-borne disease caused by protozoan parasites of the genus Leishmania. Our knowledge of protein phosphatases (PPs) and their implication in signaling events is very limited. Here we report the expression, characterization and mutagenesis analysis of a novel protein phosphatase 5 (PP5) in Leishmania major. Recombinant PP5 is a bona fide phosphatase and is enzymatically active. Site-directed mutagenesis revealed autoinhibitory roles of the N-terminal region. This is a rational first approach to understand the role of PP5 in the biology of the parasite better as well as its potential future applicability to anti-parasitic intervention
La leishmaniose est une maladie transmise par un vecteur, due a` des parasites protozoaires appartenant au genre Leishmania. Notre connaissance des phosphatases de prote´ines (PPs) et leur implication dans la signalisation est tre`s limite´e. Nous rapportons ici l’expression, la caracte´risation et les analyses de mutagene`se d’une nouvelle PP5 chez Leishmania major. La PP5 recombinante est une phosphatase authentique, enzymatiquement active. La mutagene`se dirige´e a identifie´ les roˆles auto-inhibiteurs de la re´gion N-terminale de la PP5. Il s’agit d’une premie`re approche rationnelle vers une meilleure compre´hension du roˆle de la PP5 dans la biologie du parasite, ainsi que pour une utilisation potentielle dans le cadre d’interventions antiparasitaires
Fil: Norris Mullins, Brianna. University Of Notre Dame-Indiana; Estados Unidos
Fil: Vacchina, Paola. University Of Notre Dame-Indiana; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Morales, Miguel A.. University Of Notre Dame-Indiana; Estados Unidos
Materia
SIGNALING
PHOSPHATASES
MUTAGENESIS
ACTIVITY
DRUG TARGET
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/29678
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Catalytic activity of a novel serine/threonine protein phosphatase PP5 from Leishmania majorActivite´ catalytique d’une nouvelle phosphatase de prote´ine se´rine/thre´onine PP5 de Leishmania majorNorris Mullins, BriannaVacchina, PaolaMorales, Miguel A.SIGNALINGPHOSPHATASESMUTAGENESISACTIVITYDRUG TARGEThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Leishmaniasis is a vector-borne disease caused by protozoan parasites of the genus Leishmania. Our knowledge of protein phosphatases (PPs) and their implication in signaling events is very limited. Here we report the expression, characterization and mutagenesis analysis of a novel protein phosphatase 5 (PP5) in Leishmania major. Recombinant PP5 is a bona fide phosphatase and is enzymatically active. Site-directed mutagenesis revealed autoinhibitory roles of the N-terminal region. This is a rational first approach to understand the role of PP5 in the biology of the parasite better as well as its potential future applicability to anti-parasitic interventionLa leishmaniose est une maladie transmise par un vecteur, due a` des parasites protozoaires appartenant au genre Leishmania. Notre connaissance des phosphatases de prote´ines (PPs) et leur implication dans la signalisation est tre`s limite´e. Nous rapportons ici l’expression, la caracte´risation et les analyses de mutagene`se d’une nouvelle PP5 chez Leishmania major. La PP5 recombinante est une phosphatase authentique, enzymatiquement active. La mutagene`se dirige´e a identifie´ les roˆles auto-inhibiteurs de la re´gion N-terminale de la PP5. Il s’agit d’une premie`re approche rationnelle vers une meilleure compre´hension du roˆle de la PP5 dans la biologie du parasite, ainsi que pour une utilisation potentielle dans le cadre d’interventions antiparasitairesFil: Norris Mullins, Brianna. University Of Notre Dame-Indiana; Estados UnidosFil: Vacchina, Paola. University Of Notre Dame-Indiana; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Morales, Miguel A.. University Of Notre Dame-Indiana; Estados UnidosPrinceps Editions2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29678Norris Mullins, Brianna; Vacchina, Paola; Morales, Miguel A.; Catalytic activity of a novel serine/threonine protein phosphatase PP5 from Leishmania major; Princeps Editions; Parasite-journal de la Societe Francaise de Parasitologie; 21; 6-2014; 1-61252-607XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1051/parasite/2014027info:eu-repo/semantics/altIdentifier/url/https://www.parasite-journal.org/articles/parasite/abs/2014/01/parasite140036/parasite140036.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:50:58Zoai:ri.conicet.gov.ar:11336/29678instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:50:58.387CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Catalytic activity of a novel serine/threonine protein phosphatase PP5 from Leishmania major
Activite´ catalytique d’une nouvelle phosphatase de prote´ine se´rine/thre´onine PP5 de Leishmania major
title Catalytic activity of a novel serine/threonine protein phosphatase PP5 from Leishmania major
spellingShingle Catalytic activity of a novel serine/threonine protein phosphatase PP5 from Leishmania major
Norris Mullins, Brianna
SIGNALING
PHOSPHATASES
MUTAGENESIS
ACTIVITY
DRUG TARGET
title_short Catalytic activity of a novel serine/threonine protein phosphatase PP5 from Leishmania major
title_full Catalytic activity of a novel serine/threonine protein phosphatase PP5 from Leishmania major
title_fullStr Catalytic activity of a novel serine/threonine protein phosphatase PP5 from Leishmania major
title_full_unstemmed Catalytic activity of a novel serine/threonine protein phosphatase PP5 from Leishmania major
title_sort Catalytic activity of a novel serine/threonine protein phosphatase PP5 from Leishmania major
dc.creator.none.fl_str_mv Norris Mullins, Brianna
Vacchina, Paola
Morales, Miguel A.
author Norris Mullins, Brianna
author_facet Norris Mullins, Brianna
Vacchina, Paola
Morales, Miguel A.
author_role author
author2 Vacchina, Paola
Morales, Miguel A.
author2_role author
author
dc.subject.none.fl_str_mv SIGNALING
PHOSPHATASES
MUTAGENESIS
ACTIVITY
DRUG TARGET
topic SIGNALING
PHOSPHATASES
MUTAGENESIS
ACTIVITY
DRUG TARGET
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Leishmaniasis is a vector-borne disease caused by protozoan parasites of the genus Leishmania. Our knowledge of protein phosphatases (PPs) and their implication in signaling events is very limited. Here we report the expression, characterization and mutagenesis analysis of a novel protein phosphatase 5 (PP5) in Leishmania major. Recombinant PP5 is a bona fide phosphatase and is enzymatically active. Site-directed mutagenesis revealed autoinhibitory roles of the N-terminal region. This is a rational first approach to understand the role of PP5 in the biology of the parasite better as well as its potential future applicability to anti-parasitic intervention
La leishmaniose est une maladie transmise par un vecteur, due a` des parasites protozoaires appartenant au genre Leishmania. Notre connaissance des phosphatases de prote´ines (PPs) et leur implication dans la signalisation est tre`s limite´e. Nous rapportons ici l’expression, la caracte´risation et les analyses de mutagene`se d’une nouvelle PP5 chez Leishmania major. La PP5 recombinante est une phosphatase authentique, enzymatiquement active. La mutagene`se dirige´e a identifie´ les roˆles auto-inhibiteurs de la re´gion N-terminale de la PP5. Il s’agit d’une premie`re approche rationnelle vers une meilleure compre´hension du roˆle de la PP5 dans la biologie du parasite, ainsi que pour une utilisation potentielle dans le cadre d’interventions antiparasitaires
Fil: Norris Mullins, Brianna. University Of Notre Dame-Indiana; Estados Unidos
Fil: Vacchina, Paola. University Of Notre Dame-Indiana; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Morales, Miguel A.. University Of Notre Dame-Indiana; Estados Unidos
description Leishmaniasis is a vector-borne disease caused by protozoan parasites of the genus Leishmania. Our knowledge of protein phosphatases (PPs) and their implication in signaling events is very limited. Here we report the expression, characterization and mutagenesis analysis of a novel protein phosphatase 5 (PP5) in Leishmania major. Recombinant PP5 is a bona fide phosphatase and is enzymatically active. Site-directed mutagenesis revealed autoinhibitory roles of the N-terminal region. This is a rational first approach to understand the role of PP5 in the biology of the parasite better as well as its potential future applicability to anti-parasitic intervention
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/29678
Norris Mullins, Brianna; Vacchina, Paola; Morales, Miguel A.; Catalytic activity of a novel serine/threonine protein phosphatase PP5 from Leishmania major; Princeps Editions; Parasite-journal de la Societe Francaise de Parasitologie; 21; 6-2014; 1-6
1252-607X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/29678
identifier_str_mv Norris Mullins, Brianna; Vacchina, Paola; Morales, Miguel A.; Catalytic activity of a novel serine/threonine protein phosphatase PP5 from Leishmania major; Princeps Editions; Parasite-journal de la Societe Francaise de Parasitologie; 21; 6-2014; 1-6
1252-607X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1051/parasite/2014027
info:eu-repo/semantics/altIdentifier/url/https://www.parasite-journal.org/articles/parasite/abs/2014/01/parasite140036/parasite140036.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Princeps Editions
publisher.none.fl_str_mv Princeps Editions
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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