Gut mucosal immunomodulation by probiotic fresh cheese
- Autores
- Medici, Marta Graciela; Vinderola, Celso Gabriel; Perdigon, Gabriela del Valle
- Año de publicación
- 2004
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Probiotic Fresh Cheese (PFC) is a suitable vehicle for the oral administration of Streptococcus thermophilus, Lactococcus lactis (lactic acid starter bacteria), Bifidobacterium bifidum, Lactobacillus acidophilus and L. paracasei (probiotic bacteria). PFC warrants adequate viability of the bacteria (60 days after manufacture) and protects against acidity in vitro. The aim of this work was to evaluate the effect of PFC on the mucosal immune response in vivo. BALB/c mice were fed for 2, 5 or 7 consecutive days with PFC (10 8cells/day/mouse). Mice fed with conventional balanced diet or with Control Fresh Cheese (CFC) were used as controls. The immune response (phagocytic activity of peritoneal macrophages, number of IgA+ producing cells in the small and large intestine and ratio of CD4+ and CD8+ T lymphocytes in the small intestine) was evaluated at the end of each feeding period. The presence of each probiotic bacterium and total PFC microflora as bacterial antigens in Peyer's patches or in immune cells associated with the villi of the small intestine or in nodules and crypts of the large intestine was determined by using fluorescein isothiocianate (FITC)-labelled bacteria. Histological preparations of the small and large intestine were performed 30min after the administration of FITC-labelled bacteria. A significant increase in the phagocytic activity of peritoneal macrophages, in the number of IgA+ producing cells and in the CD4+/CD8+ ratio was observed in the small intestine after 5 days treatment with PFC whereas no significant differences were observed in the large intestine. These values returned to control values 8d following PFC withdrawal. In the large intestine, no significant differences were observed respect to controls. FITC-labelled pure cultures of B. bifidum and L. paracasei were identified mainly in Peyer's patches (small intestine) whereas L. acidophilus was mainly located in the large intestine. CFC microflora was found in lower levels than PFC microflora. This study demonstrates that PFC is a dairy product that enables Bifidobacterium bifidum, Lactobacillus acidophilus and L. paracasei to exert important immunomodulating effects in the gut.
Fil: Medici, Marta Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina - Materia
-
Cfc, Control Fresh Cheese
Fitc, Fluorescein Isothiocyanate
Lactic Acid Bacteria
Mucosal Immunity
Pfc, Probiotic Fresh Cheese
Probiotic Cheese - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/58636
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CONICET Digital (CONICET) |
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Gut mucosal immunomodulation by probiotic fresh cheeseMedici, Marta GracielaVinderola, Celso GabrielPerdigon, Gabriela del ValleCfc, Control Fresh CheeseFitc, Fluorescein IsothiocyanateLactic Acid BacteriaMucosal ImmunityPfc, Probiotic Fresh CheeseProbiotic Cheesehttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Probiotic Fresh Cheese (PFC) is a suitable vehicle for the oral administration of Streptococcus thermophilus, Lactococcus lactis (lactic acid starter bacteria), Bifidobacterium bifidum, Lactobacillus acidophilus and L. paracasei (probiotic bacteria). PFC warrants adequate viability of the bacteria (60 days after manufacture) and protects against acidity in vitro. The aim of this work was to evaluate the effect of PFC on the mucosal immune response in vivo. BALB/c mice were fed for 2, 5 or 7 consecutive days with PFC (10 8cells/day/mouse). Mice fed with conventional balanced diet or with Control Fresh Cheese (CFC) were used as controls. The immune response (phagocytic activity of peritoneal macrophages, number of IgA+ producing cells in the small and large intestine and ratio of CD4+ and CD8+ T lymphocytes in the small intestine) was evaluated at the end of each feeding period. The presence of each probiotic bacterium and total PFC microflora as bacterial antigens in Peyer's patches or in immune cells associated with the villi of the small intestine or in nodules and crypts of the large intestine was determined by using fluorescein isothiocianate (FITC)-labelled bacteria. Histological preparations of the small and large intestine were performed 30min after the administration of FITC-labelled bacteria. A significant increase in the phagocytic activity of peritoneal macrophages, in the number of IgA+ producing cells and in the CD4+/CD8+ ratio was observed in the small intestine after 5 days treatment with PFC whereas no significant differences were observed in the large intestine. These values returned to control values 8d following PFC withdrawal. In the large intestine, no significant differences were observed respect to controls. FITC-labelled pure cultures of B. bifidum and L. paracasei were identified mainly in Peyer's patches (small intestine) whereas L. acidophilus was mainly located in the large intestine. CFC microflora was found in lower levels than PFC microflora. This study demonstrates that PFC is a dairy product that enables Bifidobacterium bifidum, Lactobacillus acidophilus and L. paracasei to exert important immunomodulating effects in the gut.Fil: Medici, Marta Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaElsevier2004-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/58636Medici, Marta Graciela; Vinderola, Celso Gabriel; Perdigon, Gabriela del Valle; Gut mucosal immunomodulation by probiotic fresh cheese; Elsevier; International Dairy Journal; 14; 7; 7-2004; 611-6180958-6946CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.idairyj.2003.10.011info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0958694603002607info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:55Zoai:ri.conicet.gov.ar:11336/58636instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:55.507CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Gut mucosal immunomodulation by probiotic fresh cheese |
title |
Gut mucosal immunomodulation by probiotic fresh cheese |
spellingShingle |
Gut mucosal immunomodulation by probiotic fresh cheese Medici, Marta Graciela Cfc, Control Fresh Cheese Fitc, Fluorescein Isothiocyanate Lactic Acid Bacteria Mucosal Immunity Pfc, Probiotic Fresh Cheese Probiotic Cheese |
title_short |
Gut mucosal immunomodulation by probiotic fresh cheese |
title_full |
Gut mucosal immunomodulation by probiotic fresh cheese |
title_fullStr |
Gut mucosal immunomodulation by probiotic fresh cheese |
title_full_unstemmed |
Gut mucosal immunomodulation by probiotic fresh cheese |
title_sort |
Gut mucosal immunomodulation by probiotic fresh cheese |
dc.creator.none.fl_str_mv |
Medici, Marta Graciela Vinderola, Celso Gabriel Perdigon, Gabriela del Valle |
author |
Medici, Marta Graciela |
author_facet |
Medici, Marta Graciela Vinderola, Celso Gabriel Perdigon, Gabriela del Valle |
author_role |
author |
author2 |
Vinderola, Celso Gabriel Perdigon, Gabriela del Valle |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Cfc, Control Fresh Cheese Fitc, Fluorescein Isothiocyanate Lactic Acid Bacteria Mucosal Immunity Pfc, Probiotic Fresh Cheese Probiotic Cheese |
topic |
Cfc, Control Fresh Cheese Fitc, Fluorescein Isothiocyanate Lactic Acid Bacteria Mucosal Immunity Pfc, Probiotic Fresh Cheese Probiotic Cheese |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.11 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Probiotic Fresh Cheese (PFC) is a suitable vehicle for the oral administration of Streptococcus thermophilus, Lactococcus lactis (lactic acid starter bacteria), Bifidobacterium bifidum, Lactobacillus acidophilus and L. paracasei (probiotic bacteria). PFC warrants adequate viability of the bacteria (60 days after manufacture) and protects against acidity in vitro. The aim of this work was to evaluate the effect of PFC on the mucosal immune response in vivo. BALB/c mice were fed for 2, 5 or 7 consecutive days with PFC (10 8cells/day/mouse). Mice fed with conventional balanced diet or with Control Fresh Cheese (CFC) were used as controls. The immune response (phagocytic activity of peritoneal macrophages, number of IgA+ producing cells in the small and large intestine and ratio of CD4+ and CD8+ T lymphocytes in the small intestine) was evaluated at the end of each feeding period. The presence of each probiotic bacterium and total PFC microflora as bacterial antigens in Peyer's patches or in immune cells associated with the villi of the small intestine or in nodules and crypts of the large intestine was determined by using fluorescein isothiocianate (FITC)-labelled bacteria. Histological preparations of the small and large intestine were performed 30min after the administration of FITC-labelled bacteria. A significant increase in the phagocytic activity of peritoneal macrophages, in the number of IgA+ producing cells and in the CD4+/CD8+ ratio was observed in the small intestine after 5 days treatment with PFC whereas no significant differences were observed in the large intestine. These values returned to control values 8d following PFC withdrawal. In the large intestine, no significant differences were observed respect to controls. FITC-labelled pure cultures of B. bifidum and L. paracasei were identified mainly in Peyer's patches (small intestine) whereas L. acidophilus was mainly located in the large intestine. CFC microflora was found in lower levels than PFC microflora. This study demonstrates that PFC is a dairy product that enables Bifidobacterium bifidum, Lactobacillus acidophilus and L. paracasei to exert important immunomodulating effects in the gut. Fil: Medici, Marta Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina |
description |
Probiotic Fresh Cheese (PFC) is a suitable vehicle for the oral administration of Streptococcus thermophilus, Lactococcus lactis (lactic acid starter bacteria), Bifidobacterium bifidum, Lactobacillus acidophilus and L. paracasei (probiotic bacteria). PFC warrants adequate viability of the bacteria (60 days after manufacture) and protects against acidity in vitro. The aim of this work was to evaluate the effect of PFC on the mucosal immune response in vivo. BALB/c mice were fed for 2, 5 or 7 consecutive days with PFC (10 8cells/day/mouse). Mice fed with conventional balanced diet or with Control Fresh Cheese (CFC) were used as controls. The immune response (phagocytic activity of peritoneal macrophages, number of IgA+ producing cells in the small and large intestine and ratio of CD4+ and CD8+ T lymphocytes in the small intestine) was evaluated at the end of each feeding period. The presence of each probiotic bacterium and total PFC microflora as bacterial antigens in Peyer's patches or in immune cells associated with the villi of the small intestine or in nodules and crypts of the large intestine was determined by using fluorescein isothiocianate (FITC)-labelled bacteria. Histological preparations of the small and large intestine were performed 30min after the administration of FITC-labelled bacteria. A significant increase in the phagocytic activity of peritoneal macrophages, in the number of IgA+ producing cells and in the CD4+/CD8+ ratio was observed in the small intestine after 5 days treatment with PFC whereas no significant differences were observed in the large intestine. These values returned to control values 8d following PFC withdrawal. In the large intestine, no significant differences were observed respect to controls. FITC-labelled pure cultures of B. bifidum and L. paracasei were identified mainly in Peyer's patches (small intestine) whereas L. acidophilus was mainly located in the large intestine. CFC microflora was found in lower levels than PFC microflora. This study demonstrates that PFC is a dairy product that enables Bifidobacterium bifidum, Lactobacillus acidophilus and L. paracasei to exert important immunomodulating effects in the gut. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/58636 Medici, Marta Graciela; Vinderola, Celso Gabriel; Perdigon, Gabriela del Valle; Gut mucosal immunomodulation by probiotic fresh cheese; Elsevier; International Dairy Journal; 14; 7; 7-2004; 611-618 0958-6946 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/58636 |
identifier_str_mv |
Medici, Marta Graciela; Vinderola, Celso Gabriel; Perdigon, Gabriela del Valle; Gut mucosal immunomodulation by probiotic fresh cheese; Elsevier; International Dairy Journal; 14; 7; 7-2004; 611-618 0958-6946 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.idairyj.2003.10.011 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0958694603002607 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613594024509440 |
score |
13.070432 |