Gut mucosal immunomodulation by probiotic fresh cheese

Autores
Medici, Marta Graciela; Vinderola, Celso Gabriel; Perdigon, Gabriela del Valle
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Probiotic Fresh Cheese (PFC) is a suitable vehicle for the oral administration of Streptococcus thermophilus, Lactococcus lactis (lactic acid starter bacteria), Bifidobacterium bifidum, Lactobacillus acidophilus and L. paracasei (probiotic bacteria). PFC warrants adequate viability of the bacteria (60 days after manufacture) and protects against acidity in vitro. The aim of this work was to evaluate the effect of PFC on the mucosal immune response in vivo. BALB/c mice were fed for 2, 5 or 7 consecutive days with PFC (10 8cells/day/mouse). Mice fed with conventional balanced diet or with Control Fresh Cheese (CFC) were used as controls. The immune response (phagocytic activity of peritoneal macrophages, number of IgA+ producing cells in the small and large intestine and ratio of CD4+ and CD8+ T lymphocytes in the small intestine) was evaluated at the end of each feeding period. The presence of each probiotic bacterium and total PFC microflora as bacterial antigens in Peyer's patches or in immune cells associated with the villi of the small intestine or in nodules and crypts of the large intestine was determined by using fluorescein isothiocianate (FITC)-labelled bacteria. Histological preparations of the small and large intestine were performed 30min after the administration of FITC-labelled bacteria. A significant increase in the phagocytic activity of peritoneal macrophages, in the number of IgA+ producing cells and in the CD4+/CD8+ ratio was observed in the small intestine after 5 days treatment with PFC whereas no significant differences were observed in the large intestine. These values returned to control values 8d following PFC withdrawal. In the large intestine, no significant differences were observed respect to controls. FITC-labelled pure cultures of B. bifidum and L. paracasei were identified mainly in Peyer's patches (small intestine) whereas L. acidophilus was mainly located in the large intestine. CFC microflora was found in lower levels than PFC microflora. This study demonstrates that PFC is a dairy product that enables Bifidobacterium bifidum, Lactobacillus acidophilus and L. paracasei to exert important immunomodulating effects in the gut.
Fil: Medici, Marta Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Materia
Cfc, Control Fresh Cheese
Fitc, Fluorescein Isothiocyanate
Lactic Acid Bacteria
Mucosal Immunity
Pfc, Probiotic Fresh Cheese
Probiotic Cheese
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/58636

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oai_identifier_str oai:ri.conicet.gov.ar:11336/58636
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Gut mucosal immunomodulation by probiotic fresh cheeseMedici, Marta GracielaVinderola, Celso GabrielPerdigon, Gabriela del ValleCfc, Control Fresh CheeseFitc, Fluorescein IsothiocyanateLactic Acid BacteriaMucosal ImmunityPfc, Probiotic Fresh CheeseProbiotic Cheesehttps://purl.org/becyt/ford/2.11https://purl.org/becyt/ford/2Probiotic Fresh Cheese (PFC) is a suitable vehicle for the oral administration of Streptococcus thermophilus, Lactococcus lactis (lactic acid starter bacteria), Bifidobacterium bifidum, Lactobacillus acidophilus and L. paracasei (probiotic bacteria). PFC warrants adequate viability of the bacteria (60 days after manufacture) and protects against acidity in vitro. The aim of this work was to evaluate the effect of PFC on the mucosal immune response in vivo. BALB/c mice were fed for 2, 5 or 7 consecutive days with PFC (10 8cells/day/mouse). Mice fed with conventional balanced diet or with Control Fresh Cheese (CFC) were used as controls. The immune response (phagocytic activity of peritoneal macrophages, number of IgA+ producing cells in the small and large intestine and ratio of CD4+ and CD8+ T lymphocytes in the small intestine) was evaluated at the end of each feeding period. The presence of each probiotic bacterium and total PFC microflora as bacterial antigens in Peyer's patches or in immune cells associated with the villi of the small intestine or in nodules and crypts of the large intestine was determined by using fluorescein isothiocianate (FITC)-labelled bacteria. Histological preparations of the small and large intestine were performed 30min after the administration of FITC-labelled bacteria. A significant increase in the phagocytic activity of peritoneal macrophages, in the number of IgA+ producing cells and in the CD4+/CD8+ ratio was observed in the small intestine after 5 days treatment with PFC whereas no significant differences were observed in the large intestine. These values returned to control values 8d following PFC withdrawal. In the large intestine, no significant differences were observed respect to controls. FITC-labelled pure cultures of B. bifidum and L. paracasei were identified mainly in Peyer's patches (small intestine) whereas L. acidophilus was mainly located in the large intestine. CFC microflora was found in lower levels than PFC microflora. This study demonstrates that PFC is a dairy product that enables Bifidobacterium bifidum, Lactobacillus acidophilus and L. paracasei to exert important immunomodulating effects in the gut.Fil: Medici, Marta Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaElsevier2004-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/58636Medici, Marta Graciela; Vinderola, Celso Gabriel; Perdigon, Gabriela del Valle; Gut mucosal immunomodulation by probiotic fresh cheese; Elsevier; International Dairy Journal; 14; 7; 7-2004; 611-6180958-6946CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.idairyj.2003.10.011info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0958694603002607info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:51:55Zoai:ri.conicet.gov.ar:11336/58636instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:51:55.507CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Gut mucosal immunomodulation by probiotic fresh cheese
title Gut mucosal immunomodulation by probiotic fresh cheese
spellingShingle Gut mucosal immunomodulation by probiotic fresh cheese
Medici, Marta Graciela
Cfc, Control Fresh Cheese
Fitc, Fluorescein Isothiocyanate
Lactic Acid Bacteria
Mucosal Immunity
Pfc, Probiotic Fresh Cheese
Probiotic Cheese
title_short Gut mucosal immunomodulation by probiotic fresh cheese
title_full Gut mucosal immunomodulation by probiotic fresh cheese
title_fullStr Gut mucosal immunomodulation by probiotic fresh cheese
title_full_unstemmed Gut mucosal immunomodulation by probiotic fresh cheese
title_sort Gut mucosal immunomodulation by probiotic fresh cheese
dc.creator.none.fl_str_mv Medici, Marta Graciela
Vinderola, Celso Gabriel
Perdigon, Gabriela del Valle
author Medici, Marta Graciela
author_facet Medici, Marta Graciela
Vinderola, Celso Gabriel
Perdigon, Gabriela del Valle
author_role author
author2 Vinderola, Celso Gabriel
Perdigon, Gabriela del Valle
author2_role author
author
dc.subject.none.fl_str_mv Cfc, Control Fresh Cheese
Fitc, Fluorescein Isothiocyanate
Lactic Acid Bacteria
Mucosal Immunity
Pfc, Probiotic Fresh Cheese
Probiotic Cheese
topic Cfc, Control Fresh Cheese
Fitc, Fluorescein Isothiocyanate
Lactic Acid Bacteria
Mucosal Immunity
Pfc, Probiotic Fresh Cheese
Probiotic Cheese
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.11
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Probiotic Fresh Cheese (PFC) is a suitable vehicle for the oral administration of Streptococcus thermophilus, Lactococcus lactis (lactic acid starter bacteria), Bifidobacterium bifidum, Lactobacillus acidophilus and L. paracasei (probiotic bacteria). PFC warrants adequate viability of the bacteria (60 days after manufacture) and protects against acidity in vitro. The aim of this work was to evaluate the effect of PFC on the mucosal immune response in vivo. BALB/c mice were fed for 2, 5 or 7 consecutive days with PFC (10 8cells/day/mouse). Mice fed with conventional balanced diet or with Control Fresh Cheese (CFC) were used as controls. The immune response (phagocytic activity of peritoneal macrophages, number of IgA+ producing cells in the small and large intestine and ratio of CD4+ and CD8+ T lymphocytes in the small intestine) was evaluated at the end of each feeding period. The presence of each probiotic bacterium and total PFC microflora as bacterial antigens in Peyer's patches or in immune cells associated with the villi of the small intestine or in nodules and crypts of the large intestine was determined by using fluorescein isothiocianate (FITC)-labelled bacteria. Histological preparations of the small and large intestine were performed 30min after the administration of FITC-labelled bacteria. A significant increase in the phagocytic activity of peritoneal macrophages, in the number of IgA+ producing cells and in the CD4+/CD8+ ratio was observed in the small intestine after 5 days treatment with PFC whereas no significant differences were observed in the large intestine. These values returned to control values 8d following PFC withdrawal. In the large intestine, no significant differences were observed respect to controls. FITC-labelled pure cultures of B. bifidum and L. paracasei were identified mainly in Peyer's patches (small intestine) whereas L. acidophilus was mainly located in the large intestine. CFC microflora was found in lower levels than PFC microflora. This study demonstrates that PFC is a dairy product that enables Bifidobacterium bifidum, Lactobacillus acidophilus and L. paracasei to exert important immunomodulating effects in the gut.
Fil: Medici, Marta Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
description Probiotic Fresh Cheese (PFC) is a suitable vehicle for the oral administration of Streptococcus thermophilus, Lactococcus lactis (lactic acid starter bacteria), Bifidobacterium bifidum, Lactobacillus acidophilus and L. paracasei (probiotic bacteria). PFC warrants adequate viability of the bacteria (60 days after manufacture) and protects against acidity in vitro. The aim of this work was to evaluate the effect of PFC on the mucosal immune response in vivo. BALB/c mice were fed for 2, 5 or 7 consecutive days with PFC (10 8cells/day/mouse). Mice fed with conventional balanced diet or with Control Fresh Cheese (CFC) were used as controls. The immune response (phagocytic activity of peritoneal macrophages, number of IgA+ producing cells in the small and large intestine and ratio of CD4+ and CD8+ T lymphocytes in the small intestine) was evaluated at the end of each feeding period. The presence of each probiotic bacterium and total PFC microflora as bacterial antigens in Peyer's patches or in immune cells associated with the villi of the small intestine or in nodules and crypts of the large intestine was determined by using fluorescein isothiocianate (FITC)-labelled bacteria. Histological preparations of the small and large intestine were performed 30min after the administration of FITC-labelled bacteria. A significant increase in the phagocytic activity of peritoneal macrophages, in the number of IgA+ producing cells and in the CD4+/CD8+ ratio was observed in the small intestine after 5 days treatment with PFC whereas no significant differences were observed in the large intestine. These values returned to control values 8d following PFC withdrawal. In the large intestine, no significant differences were observed respect to controls. FITC-labelled pure cultures of B. bifidum and L. paracasei were identified mainly in Peyer's patches (small intestine) whereas L. acidophilus was mainly located in the large intestine. CFC microflora was found in lower levels than PFC microflora. This study demonstrates that PFC is a dairy product that enables Bifidobacterium bifidum, Lactobacillus acidophilus and L. paracasei to exert important immunomodulating effects in the gut.
publishDate 2004
dc.date.none.fl_str_mv 2004-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/58636
Medici, Marta Graciela; Vinderola, Celso Gabriel; Perdigon, Gabriela del Valle; Gut mucosal immunomodulation by probiotic fresh cheese; Elsevier; International Dairy Journal; 14; 7; 7-2004; 611-618
0958-6946
CONICET Digital
CONICET
url http://hdl.handle.net/11336/58636
identifier_str_mv Medici, Marta Graciela; Vinderola, Celso Gabriel; Perdigon, Gabriela del Valle; Gut mucosal immunomodulation by probiotic fresh cheese; Elsevier; International Dairy Journal; 14; 7; 7-2004; 611-618
0958-6946
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.idairyj.2003.10.011
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0958694603002607
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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