Ptr/CTL0175 is required for the efficient recovery of chlamydia trachomatisfrom stress induced by gamma-interferon

Autores
Panzetta, Maria Emilia; Lujan, Agustin Leonardo; Bastidas, Robert J.; Damiani, Maria Teresa; Valdivia, Raphael H.; Saka, Hector Alex
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chlamydia trachomatis is the most common sexually transmitted bacterial pathogen in humans and a frequent cause of asymptomatic, persistent infections leading to serious complications, particularly in young women. Chlamydia displays a unique obligate intracellular lifestyle involving the infectious elementary body and the replicative reticulate body. In the presence of stressors such as gamma-interferon (IFNγ) or beta-lactam antibiotics, C. trachomatis undergoes an interruption in its replication cycle and enters a viable but non-cultivable state. Upon removal of the stressors, surviving C. trachomatis resume cell division and developmental transitions. In this report, we describe a genetic screen to identify C. trachomatis mutants with defects in recovery from IFNγ- and/or penicillin-induced stress and characterized a chemically derived C. trachomatis mutant strain that exhibited a significant decrease in recovery from IFNγ- but not penicillin-induced stress. Through lateral gene transfer and targeted insertional gene inactivation we identified ptr, encoding a predicted protease, as a gene required for recovery from IFNγ-induced stress. A C. trachomatis LGV-L2 ptr-null strain displayed reduced generation of infectious progeny and impaired genome replication upon removal of IFNγ. This defect was restored by introducing a wild type copy of ptr on a plasmid, indicating that Ptr is required for a rapid growth upon removal of IFN?. Ptr was expressed throughout the developmental cycle and localized to the inclusion lumen. Overall, our findings indicate that the putative secreted protease Ptr is required for C. trachomatis to specifically recover from IFNγ- but not penicillin-induced stress.
Fil: Panzetta, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Lujan, Agustin Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Bastidas, Robert J.. University of Duke; Estados Unidos
Fil: Damiani, Maria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Valdivia, Raphael H.. University of Duke; Estados Unidos
Fil: Saka, Hector Alex. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Materia
CHLAMYDIA TRACHOMATIS
IFNΓ
IFNΓ-INDUCED STRESS
PENICILLIN
PERSISTENCE
PTR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/128472
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/128472
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ptr/CTL0175 is required for the efficient recovery of chlamydia trachomatisfrom stress induced by gamma-interferonPanzetta, Maria EmiliaLujan, Agustin LeonardoBastidas, Robert J.Damiani, Maria TeresaValdivia, Raphael H.Saka, Hector AlexCHLAMYDIA TRACHOMATISIFNΓIFNΓ-INDUCED STRESSPENICILLINPERSISTENCEPTRhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Chlamydia trachomatis is the most common sexually transmitted bacterial pathogen in humans and a frequent cause of asymptomatic, persistent infections leading to serious complications, particularly in young women. Chlamydia displays a unique obligate intracellular lifestyle involving the infectious elementary body and the replicative reticulate body. In the presence of stressors such as gamma-interferon (IFNγ) or beta-lactam antibiotics, C. trachomatis undergoes an interruption in its replication cycle and enters a viable but non-cultivable state. Upon removal of the stressors, surviving C. trachomatis resume cell division and developmental transitions. In this report, we describe a genetic screen to identify C. trachomatis mutants with defects in recovery from IFNγ- and/or penicillin-induced stress and characterized a chemically derived C. trachomatis mutant strain that exhibited a significant decrease in recovery from IFNγ- but not penicillin-induced stress. Through lateral gene transfer and targeted insertional gene inactivation we identified ptr, encoding a predicted protease, as a gene required for recovery from IFNγ-induced stress. A C. trachomatis LGV-L2 ptr-null strain displayed reduced generation of infectious progeny and impaired genome replication upon removal of IFNγ. This defect was restored by introducing a wild type copy of ptr on a plasmid, indicating that Ptr is required for a rapid growth upon removal of IFN?. Ptr was expressed throughout the developmental cycle and localized to the inclusion lumen. Overall, our findings indicate that the putative secreted protease Ptr is required for C. trachomatis to specifically recover from IFNγ- but not penicillin-induced stress.Fil: Panzetta, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Lujan, Agustin Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Bastidas, Robert J.. University of Duke; Estados UnidosFil: Damiani, Maria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Valdivia, Raphael H.. University of Duke; Estados UnidosFil: Saka, Hector Alex. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFrontiers Media S.A.2019-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/128472Panzetta, Maria Emilia; Lujan, Agustin Leonardo; Bastidas, Robert J.; Damiani, Maria Teresa; Valdivia, Raphael H.; et al.; Ptr/CTL0175 is required for the efficient recovery of chlamydia trachomatisfrom stress induced by gamma-interferon; Frontiers Media S.A.; Frontiers in Microbiology; 10; 4-2019; 1-181664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2019.00756/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.00756info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:36:29Zoai:ri.conicet.gov.ar:11336/128472instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:36:30.268CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ptr/CTL0175 is required for the efficient recovery of chlamydia trachomatisfrom stress induced by gamma-interferon
title Ptr/CTL0175 is required for the efficient recovery of chlamydia trachomatisfrom stress induced by gamma-interferon
spellingShingle Ptr/CTL0175 is required for the efficient recovery of chlamydia trachomatisfrom stress induced by gamma-interferon
Panzetta, Maria Emilia
CHLAMYDIA TRACHOMATIS
IFNΓ
IFNΓ-INDUCED STRESS
PENICILLIN
PERSISTENCE
PTR
title_short Ptr/CTL0175 is required for the efficient recovery of chlamydia trachomatisfrom stress induced by gamma-interferon
title_full Ptr/CTL0175 is required for the efficient recovery of chlamydia trachomatisfrom stress induced by gamma-interferon
title_fullStr Ptr/CTL0175 is required for the efficient recovery of chlamydia trachomatisfrom stress induced by gamma-interferon
title_full_unstemmed Ptr/CTL0175 is required for the efficient recovery of chlamydia trachomatisfrom stress induced by gamma-interferon
title_sort Ptr/CTL0175 is required for the efficient recovery of chlamydia trachomatisfrom stress induced by gamma-interferon
dc.creator.none.fl_str_mv Panzetta, Maria Emilia
Lujan, Agustin Leonardo
Bastidas, Robert J.
Damiani, Maria Teresa
Valdivia, Raphael H.
Saka, Hector Alex
author Panzetta, Maria Emilia
author_facet Panzetta, Maria Emilia
Lujan, Agustin Leonardo
Bastidas, Robert J.
Damiani, Maria Teresa
Valdivia, Raphael H.
Saka, Hector Alex
author_role author
author2 Lujan, Agustin Leonardo
Bastidas, Robert J.
Damiani, Maria Teresa
Valdivia, Raphael H.
Saka, Hector Alex
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CHLAMYDIA TRACHOMATIS
IFNΓ
IFNΓ-INDUCED STRESS
PENICILLIN
PERSISTENCE
PTR
topic CHLAMYDIA TRACHOMATIS
IFNΓ
IFNΓ-INDUCED STRESS
PENICILLIN
PERSISTENCE
PTR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Chlamydia trachomatis is the most common sexually transmitted bacterial pathogen in humans and a frequent cause of asymptomatic, persistent infections leading to serious complications, particularly in young women. Chlamydia displays a unique obligate intracellular lifestyle involving the infectious elementary body and the replicative reticulate body. In the presence of stressors such as gamma-interferon (IFNγ) or beta-lactam antibiotics, C. trachomatis undergoes an interruption in its replication cycle and enters a viable but non-cultivable state. Upon removal of the stressors, surviving C. trachomatis resume cell division and developmental transitions. In this report, we describe a genetic screen to identify C. trachomatis mutants with defects in recovery from IFNγ- and/or penicillin-induced stress and characterized a chemically derived C. trachomatis mutant strain that exhibited a significant decrease in recovery from IFNγ- but not penicillin-induced stress. Through lateral gene transfer and targeted insertional gene inactivation we identified ptr, encoding a predicted protease, as a gene required for recovery from IFNγ-induced stress. A C. trachomatis LGV-L2 ptr-null strain displayed reduced generation of infectious progeny and impaired genome replication upon removal of IFNγ. This defect was restored by introducing a wild type copy of ptr on a plasmid, indicating that Ptr is required for a rapid growth upon removal of IFN?. Ptr was expressed throughout the developmental cycle and localized to the inclusion lumen. Overall, our findings indicate that the putative secreted protease Ptr is required for C. trachomatis to specifically recover from IFNγ- but not penicillin-induced stress.
Fil: Panzetta, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Lujan, Agustin Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Bastidas, Robert J.. University of Duke; Estados Unidos
Fil: Damiani, Maria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentina. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Valdivia, Raphael H.. University of Duke; Estados Unidos
Fil: Saka, Hector Alex. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
description Chlamydia trachomatis is the most common sexually transmitted bacterial pathogen in humans and a frequent cause of asymptomatic, persistent infections leading to serious complications, particularly in young women. Chlamydia displays a unique obligate intracellular lifestyle involving the infectious elementary body and the replicative reticulate body. In the presence of stressors such as gamma-interferon (IFNγ) or beta-lactam antibiotics, C. trachomatis undergoes an interruption in its replication cycle and enters a viable but non-cultivable state. Upon removal of the stressors, surviving C. trachomatis resume cell division and developmental transitions. In this report, we describe a genetic screen to identify C. trachomatis mutants with defects in recovery from IFNγ- and/or penicillin-induced stress and characterized a chemically derived C. trachomatis mutant strain that exhibited a significant decrease in recovery from IFNγ- but not penicillin-induced stress. Through lateral gene transfer and targeted insertional gene inactivation we identified ptr, encoding a predicted protease, as a gene required for recovery from IFNγ-induced stress. A C. trachomatis LGV-L2 ptr-null strain displayed reduced generation of infectious progeny and impaired genome replication upon removal of IFNγ. This defect was restored by introducing a wild type copy of ptr on a plasmid, indicating that Ptr is required for a rapid growth upon removal of IFN?. Ptr was expressed throughout the developmental cycle and localized to the inclusion lumen. Overall, our findings indicate that the putative secreted protease Ptr is required for C. trachomatis to specifically recover from IFNγ- but not penicillin-induced stress.
publishDate 2019
dc.date.none.fl_str_mv 2019-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/128472
Panzetta, Maria Emilia; Lujan, Agustin Leonardo; Bastidas, Robert J.; Damiani, Maria Teresa; Valdivia, Raphael H.; et al.; Ptr/CTL0175 is required for the efficient recovery of chlamydia trachomatisfrom stress induced by gamma-interferon; Frontiers Media S.A.; Frontiers in Microbiology; 10; 4-2019; 1-18
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/128472
identifier_str_mv Panzetta, Maria Emilia; Lujan, Agustin Leonardo; Bastidas, Robert J.; Damiani, Maria Teresa; Valdivia, Raphael H.; et al.; Ptr/CTL0175 is required for the efficient recovery of chlamydia trachomatisfrom stress induced by gamma-interferon; Frontiers Media S.A.; Frontiers in Microbiology; 10; 4-2019; 1-18
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/article/10.3389/fmicb.2019.00756/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2019.00756
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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