Influence of the Glass Transition on Rotational Dynamics of Dyes in Thin Polymer Films. Single Molecule and Ensemble Experiments

Autores
Araoz, Beatriz; Carattino, Aquiles; Täuber, Daniela; Von Borczyskowski, Christian; Aramendia, Pedro Francisco
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We performed polarized fluorescence emission studies of Nile Red (NR) in poly(methyl methacrylate) (PMMA), poly(ethyl methacrylate) (PEMA), and poly(butyl methacrylate) (PBMA) at the single molecule (SM) and at the ensemble level to study the in cage movements of the ground-state molecule in polymer films of nanometric thickness at room temperature. Experiments were performed with wide field irradiation. At the ensemble level, the linearly polarized irradiation was used to induce a photoselection by bleaching, which is compensated by rotational diffusion. Both results show an appreciable difference in mobility of NR in the films that is correlated with the different glass-transition temperatures of the films, particularly in PEMA, which displays a clearly distinct behavior between the 200 nm films, representing a rigid environment, and the 25 nm ones, showing much higher mobility. We developed a model of broad application for polarized photobleaching that allows obtaining rotational diffusion coefficients and photobleaching quantum yields in an easy way from ensemble experiments. The parameters obtained from ensemble measurements correlate well with the results from SM experiments.
Fil: Araoz, Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Carattino, Aquiles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Täuber, Daniela. Technische Universitat Chemnitz; Alemania
Fil: Von Borczyskowski, Christian. Technische Universitat Chemnitz; Alemania
Fil: Aramendia, Pedro Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Materia
Single Molecule Fluorescence
Emission Anisotropy
Photobleaching
Confinement Effects
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12446
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Influence of the Glass Transition on Rotational Dynamics of Dyes in Thin Polymer Films. Single Molecule and Ensemble ExperimentsAraoz, BeatrizCarattino, AquilesTäuber, DanielaVon Borczyskowski, ChristianAramendia, Pedro FranciscoSingle Molecule FluorescenceEmission AnisotropyPhotobleachingConfinement Effectshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We performed polarized fluorescence emission studies of Nile Red (NR) in poly(methyl methacrylate) (PMMA), poly(ethyl methacrylate) (PEMA), and poly(butyl methacrylate) (PBMA) at the single molecule (SM) and at the ensemble level to study the in cage movements of the ground-state molecule in polymer films of nanometric thickness at room temperature. Experiments were performed with wide field irradiation. At the ensemble level, the linearly polarized irradiation was used to induce a photoselection by bleaching, which is compensated by rotational diffusion. Both results show an appreciable difference in mobility of NR in the films that is correlated with the different glass-transition temperatures of the films, particularly in PEMA, which displays a clearly distinct behavior between the 200 nm films, representing a rigid environment, and the 25 nm ones, showing much higher mobility. We developed a model of broad application for polarized photobleaching that allows obtaining rotational diffusion coefficients and photobleaching quantum yields in an easy way from ensemble experiments. The parameters obtained from ensemble measurements correlate well with the results from SM experiments.Fil: Araoz, Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Carattino, Aquiles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Täuber, Daniela. Technische Universitat Chemnitz; AlemaniaFil: Von Borczyskowski, Christian. Technische Universitat Chemnitz; AlemaniaFil: Aramendia, Pedro Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaAmerican Chemical Society2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12446Araoz, Beatriz; Carattino, Aquiles; Täuber, Daniela; Von Borczyskowski, Christian; Aramendia, Pedro Francisco; Influence of the Glass Transition on Rotational Dynamics of Dyes in Thin Polymer Films. Single Molecule and Ensemble Experiments; American Chemical Society; Journal Of Physical Chemistry A; 118; 45; 8-2014; 10309-103171089-5639enginfo:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/jp500272yinfo:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1021/jp500272yinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:11:17Zoai:ri.conicet.gov.ar:11336/12446instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:11:18.161CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Influence of the Glass Transition on Rotational Dynamics of Dyes in Thin Polymer Films. Single Molecule and Ensemble Experiments
title Influence of the Glass Transition on Rotational Dynamics of Dyes in Thin Polymer Films. Single Molecule and Ensemble Experiments
spellingShingle Influence of the Glass Transition on Rotational Dynamics of Dyes in Thin Polymer Films. Single Molecule and Ensemble Experiments
Araoz, Beatriz
Single Molecule Fluorescence
Emission Anisotropy
Photobleaching
Confinement Effects
title_short Influence of the Glass Transition on Rotational Dynamics of Dyes in Thin Polymer Films. Single Molecule and Ensemble Experiments
title_full Influence of the Glass Transition on Rotational Dynamics of Dyes in Thin Polymer Films. Single Molecule and Ensemble Experiments
title_fullStr Influence of the Glass Transition on Rotational Dynamics of Dyes in Thin Polymer Films. Single Molecule and Ensemble Experiments
title_full_unstemmed Influence of the Glass Transition on Rotational Dynamics of Dyes in Thin Polymer Films. Single Molecule and Ensemble Experiments
title_sort Influence of the Glass Transition on Rotational Dynamics of Dyes in Thin Polymer Films. Single Molecule and Ensemble Experiments
dc.creator.none.fl_str_mv Araoz, Beatriz
Carattino, Aquiles
Täuber, Daniela
Von Borczyskowski, Christian
Aramendia, Pedro Francisco
author Araoz, Beatriz
author_facet Araoz, Beatriz
Carattino, Aquiles
Täuber, Daniela
Von Borczyskowski, Christian
Aramendia, Pedro Francisco
author_role author
author2 Carattino, Aquiles
Täuber, Daniela
Von Borczyskowski, Christian
Aramendia, Pedro Francisco
author2_role author
author
author
author
dc.subject.none.fl_str_mv Single Molecule Fluorescence
Emission Anisotropy
Photobleaching
Confinement Effects
topic Single Molecule Fluorescence
Emission Anisotropy
Photobleaching
Confinement Effects
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We performed polarized fluorescence emission studies of Nile Red (NR) in poly(methyl methacrylate) (PMMA), poly(ethyl methacrylate) (PEMA), and poly(butyl methacrylate) (PBMA) at the single molecule (SM) and at the ensemble level to study the in cage movements of the ground-state molecule in polymer films of nanometric thickness at room temperature. Experiments were performed with wide field irradiation. At the ensemble level, the linearly polarized irradiation was used to induce a photoselection by bleaching, which is compensated by rotational diffusion. Both results show an appreciable difference in mobility of NR in the films that is correlated with the different glass-transition temperatures of the films, particularly in PEMA, which displays a clearly distinct behavior between the 200 nm films, representing a rigid environment, and the 25 nm ones, showing much higher mobility. We developed a model of broad application for polarized photobleaching that allows obtaining rotational diffusion coefficients and photobleaching quantum yields in an easy way from ensemble experiments. The parameters obtained from ensemble measurements correlate well with the results from SM experiments.
Fil: Araoz, Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Carattino, Aquiles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Täuber, Daniela. Technische Universitat Chemnitz; Alemania
Fil: Von Borczyskowski, Christian. Technische Universitat Chemnitz; Alemania
Fil: Aramendia, Pedro Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias "Elizabeth Jares Erijman"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
description We performed polarized fluorescence emission studies of Nile Red (NR) in poly(methyl methacrylate) (PMMA), poly(ethyl methacrylate) (PEMA), and poly(butyl methacrylate) (PBMA) at the single molecule (SM) and at the ensemble level to study the in cage movements of the ground-state molecule in polymer films of nanometric thickness at room temperature. Experiments were performed with wide field irradiation. At the ensemble level, the linearly polarized irradiation was used to induce a photoselection by bleaching, which is compensated by rotational diffusion. Both results show an appreciable difference in mobility of NR in the films that is correlated with the different glass-transition temperatures of the films, particularly in PEMA, which displays a clearly distinct behavior between the 200 nm films, representing a rigid environment, and the 25 nm ones, showing much higher mobility. We developed a model of broad application for polarized photobleaching that allows obtaining rotational diffusion coefficients and photobleaching quantum yields in an easy way from ensemble experiments. The parameters obtained from ensemble measurements correlate well with the results from SM experiments.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12446
Araoz, Beatriz; Carattino, Aquiles; Täuber, Daniela; Von Borczyskowski, Christian; Aramendia, Pedro Francisco; Influence of the Glass Transition on Rotational Dynamics of Dyes in Thin Polymer Films. Single Molecule and Ensemble Experiments; American Chemical Society; Journal Of Physical Chemistry A; 118; 45; 8-2014; 10309-10317
1089-5639
url http://hdl.handle.net/11336/12446
identifier_str_mv Araoz, Beatriz; Carattino, Aquiles; Täuber, Daniela; Von Borczyskowski, Christian; Aramendia, Pedro Francisco; Influence of the Glass Transition on Rotational Dynamics of Dyes in Thin Polymer Films. Single Molecule and Ensemble Experiments; American Chemical Society; Journal Of Physical Chemistry A; 118; 45; 8-2014; 10309-10317
1089-5639
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pubs.acs.org/doi/abs/10.1021/jp500272y
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1021/jp500272y
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083261039640576
score 13.22299

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