Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity

Autores
Barril, Patricia Angelica; Fumian, T. M.; Prez, Verónica Emilse; Gil, Pedro Ignacio; Martínez, L. C.; Giordano, Miguel Oscar; Masachessi, Gisela; Isa, Maria Beatriz; Ferreyra, Leonardo Jesús; Ré, Viviana Elizabeth; Miagostovich, M.; Pavan, Jorge Victorio; Nates, Silvia Viviana
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Argentina, the rotavirus disease exhibits seasonal variations, being most prevalent in the fall and winter months. To deepen the understanding of rotavirus seasonality in our community, the influence of meteorological factors on the rotavirus load and the genetic diversity in urban raw sewage from Córdoba city, Argentina were evaluated. Wastewater samples were collected monthly during a three-year study period and viral particles were concentrated by polyethylene glycol precipitation. RT-nested PCR was applied for rotavirus detection, and VP7/VP4 characterization and real-time PCR for rotavirus quantification. Both molecular techniques showed relatively similar sensitivity rates and revealed rotavirus presence in urban wastewater in cold and warm seasons, indicating its circulation in the local community all year round. However, a slight trend for rotavirus circulation was noted by real-time PCR in the fall and winter seasons, showing a significantly higher peak of rotavirus concentration at mean temperatures lower than 18. °C and also higher, although not statistically different during drier weather. VP7 and VP4 gene characterization showed that G1 and P[8] genotypes were dominant, and temporal variations in genotype distribution were not observed. Rotavirus spread is complex and our results point out that weather factors alone cannot explain the seasonal quantitative pattern of the rotavirus disease. Therefore, alternative transmission routes, changes in human behavior and susceptibility, and the stability and survivability of the virus might all together contribute to the seasonality of rotavirus. The results obtained here provide evidence regarding the dynamics of rotavirus circulation and maintenance in Argentina.
Fil: Barril, Patricia Angelica. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Fumian, T. M.. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz; Brasil
Fil: Prez, Verónica Emilse. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Fil: Gil, Pedro Ignacio. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Martínez, L. C.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Giordano, M.O.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Masachessi, Gisela. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Isa, Maria Beatriz. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Ferreyra, Leonardo Jesús. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Ré, Viviana Elizabeth. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Miagostovich, M.. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz; Brasil
Fil: Pavan, Jorge Victorio. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Nates, Silvia Viviana. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Materia
Environmental Monitoring
Meteorological Variables
Rotavirus
Seasonality
Sewage
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/61497
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/61497
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversityBarril, Patricia AngelicaFumian, T. M.Prez, Verónica EmilseGil, Pedro IgnacioMartínez, L. C.Giordano, Miguel OscarMasachessi, GiselaIsa, Maria BeatrizFerreyra, Leonardo JesúsRé, Viviana ElizabethMiagostovich, M.Pavan, Jorge VictorioNates, Silvia VivianaEnvironmental MonitoringMeteorological VariablesRotavirusSeasonalitySewagehttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3In Argentina, the rotavirus disease exhibits seasonal variations, being most prevalent in the fall and winter months. To deepen the understanding of rotavirus seasonality in our community, the influence of meteorological factors on the rotavirus load and the genetic diversity in urban raw sewage from Córdoba city, Argentina were evaluated. Wastewater samples were collected monthly during a three-year study period and viral particles were concentrated by polyethylene glycol precipitation. RT-nested PCR was applied for rotavirus detection, and VP7/VP4 characterization and real-time PCR for rotavirus quantification. Both molecular techniques showed relatively similar sensitivity rates and revealed rotavirus presence in urban wastewater in cold and warm seasons, indicating its circulation in the local community all year round. However, a slight trend for rotavirus circulation was noted by real-time PCR in the fall and winter seasons, showing a significantly higher peak of rotavirus concentration at mean temperatures lower than 18. °C and also higher, although not statistically different during drier weather. VP7 and VP4 gene characterization showed that G1 and P[8] genotypes were dominant, and temporal variations in genotype distribution were not observed. Rotavirus spread is complex and our results point out that weather factors alone cannot explain the seasonal quantitative pattern of the rotavirus disease. Therefore, alternative transmission routes, changes in human behavior and susceptibility, and the stability and survivability of the virus might all together contribute to the seasonality of rotavirus. The results obtained here provide evidence regarding the dynamics of rotavirus circulation and maintenance in Argentina.Fil: Barril, Patricia Angelica. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Fumian, T. M.. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz; BrasilFil: Prez, Verónica Emilse. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; ArgentinaFil: Gil, Pedro Ignacio. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Martínez, L. C.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Giordano, M.O.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Masachessi, Gisela. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Isa, Maria Beatriz. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Ferreyra, Leonardo Jesús. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Ré, Viviana Elizabeth. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Miagostovich, M.. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz; BrasilFil: Pavan, Jorge Victorio. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaFil: Nates, Silvia Viviana. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; ArgentinaAcademic Press Inc Elsevier Science2015-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/61497Barril, Patricia Angelica; Fumian, T. M.; Prez, Verónica Emilse; Gil, Pedro Ignacio; Martínez, L. C.; et al.; Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity; Academic Press Inc Elsevier Science; Environmental Research; 138; 4-2015; 409-4150013-9351CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/25777068info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envres.2015.03.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:45:04Zoai:ri.conicet.gov.ar:11336/61497instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:45:05.263CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity
title Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity
spellingShingle Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity
Barril, Patricia Angelica
Environmental Monitoring
Meteorological Variables
Rotavirus
Seasonality
Sewage
title_short Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity
title_full Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity
title_fullStr Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity
title_full_unstemmed Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity
title_sort Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity
dc.creator.none.fl_str_mv Barril, Patricia Angelica
Fumian, T. M.
Prez, Verónica Emilse
Gil, Pedro Ignacio
Martínez, L. C.
Giordano, Miguel Oscar
Masachessi, Gisela
Isa, Maria Beatriz
Ferreyra, Leonardo Jesús
Ré, Viviana Elizabeth
Miagostovich, M.
Pavan, Jorge Victorio
Nates, Silvia Viviana
author Barril, Patricia Angelica
author_facet Barril, Patricia Angelica
Fumian, T. M.
Prez, Verónica Emilse
Gil, Pedro Ignacio
Martínez, L. C.
Giordano, Miguel Oscar
Masachessi, Gisela
Isa, Maria Beatriz
Ferreyra, Leonardo Jesús
Ré, Viviana Elizabeth
Miagostovich, M.
Pavan, Jorge Victorio
Nates, Silvia Viviana
author_role author
author2 Fumian, T. M.
Prez, Verónica Emilse
Gil, Pedro Ignacio
Martínez, L. C.
Giordano, Miguel Oscar
Masachessi, Gisela
Isa, Maria Beatriz
Ferreyra, Leonardo Jesús
Ré, Viviana Elizabeth
Miagostovich, M.
Pavan, Jorge Victorio
Nates, Silvia Viviana
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Environmental Monitoring
Meteorological Variables
Rotavirus
Seasonality
Sewage
topic Environmental Monitoring
Meteorological Variables
Rotavirus
Seasonality
Sewage
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In Argentina, the rotavirus disease exhibits seasonal variations, being most prevalent in the fall and winter months. To deepen the understanding of rotavirus seasonality in our community, the influence of meteorological factors on the rotavirus load and the genetic diversity in urban raw sewage from Córdoba city, Argentina were evaluated. Wastewater samples were collected monthly during a three-year study period and viral particles were concentrated by polyethylene glycol precipitation. RT-nested PCR was applied for rotavirus detection, and VP7/VP4 characterization and real-time PCR for rotavirus quantification. Both molecular techniques showed relatively similar sensitivity rates and revealed rotavirus presence in urban wastewater in cold and warm seasons, indicating its circulation in the local community all year round. However, a slight trend for rotavirus circulation was noted by real-time PCR in the fall and winter seasons, showing a significantly higher peak of rotavirus concentration at mean temperatures lower than 18. °C and also higher, although not statistically different during drier weather. VP7 and VP4 gene characterization showed that G1 and P[8] genotypes were dominant, and temporal variations in genotype distribution were not observed. Rotavirus spread is complex and our results point out that weather factors alone cannot explain the seasonal quantitative pattern of the rotavirus disease. Therefore, alternative transmission routes, changes in human behavior and susceptibility, and the stability and survivability of the virus might all together contribute to the seasonality of rotavirus. The results obtained here provide evidence regarding the dynamics of rotavirus circulation and maintenance in Argentina.
Fil: Barril, Patricia Angelica. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Fumian, T. M.. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz; Brasil
Fil: Prez, Verónica Emilse. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología "Dr. J. M. Vanella"; Argentina
Fil: Gil, Pedro Ignacio. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Martínez, L. C.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Giordano, M.O.. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Masachessi, Gisela. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Isa, Maria Beatriz. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Ferreyra, Leonardo Jesús. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Ré, Viviana Elizabeth. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Miagostovich, M.. Fundação Oswaldo Cruz. Instituto Oswaldo Cruz; Brasil
Fil: Pavan, Jorge Victorio. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
Fil: Nates, Silvia Viviana. Universidad Nacional de Córdoba. Facultad de Medicina. Instituto de Virología ; Argentina
description In Argentina, the rotavirus disease exhibits seasonal variations, being most prevalent in the fall and winter months. To deepen the understanding of rotavirus seasonality in our community, the influence of meteorological factors on the rotavirus load and the genetic diversity in urban raw sewage from Córdoba city, Argentina were evaluated. Wastewater samples were collected monthly during a three-year study period and viral particles were concentrated by polyethylene glycol precipitation. RT-nested PCR was applied for rotavirus detection, and VP7/VP4 characterization and real-time PCR for rotavirus quantification. Both molecular techniques showed relatively similar sensitivity rates and revealed rotavirus presence in urban wastewater in cold and warm seasons, indicating its circulation in the local community all year round. However, a slight trend for rotavirus circulation was noted by real-time PCR in the fall and winter seasons, showing a significantly higher peak of rotavirus concentration at mean temperatures lower than 18. °C and also higher, although not statistically different during drier weather. VP7 and VP4 gene characterization showed that G1 and P[8] genotypes were dominant, and temporal variations in genotype distribution were not observed. Rotavirus spread is complex and our results point out that weather factors alone cannot explain the seasonal quantitative pattern of the rotavirus disease. Therefore, alternative transmission routes, changes in human behavior and susceptibility, and the stability and survivability of the virus might all together contribute to the seasonality of rotavirus. The results obtained here provide evidence regarding the dynamics of rotavirus circulation and maintenance in Argentina.
publishDate 2015
dc.date.none.fl_str_mv 2015-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/61497
Barril, Patricia Angelica; Fumian, T. M.; Prez, Verónica Emilse; Gil, Pedro Ignacio; Martínez, L. C.; et al.; Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity; Academic Press Inc Elsevier Science; Environmental Research; 138; 4-2015; 409-415
0013-9351
CONICET Digital
CONICET
url http://hdl.handle.net/11336/61497
identifier_str_mv Barril, Patricia Angelica; Fumian, T. M.; Prez, Verónica Emilse; Gil, Pedro Ignacio; Martínez, L. C.; et al.; Rotavirus seasonality in urban sewage from Argentina: Effect of meteorological variables on the viral load and the genetic diversity; Academic Press Inc Elsevier Science; Environmental Research; 138; 4-2015; 409-415
0013-9351
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pubmed/25777068
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envres.2015.03.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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