A One-Week Laboratory Practice: Introducing the Students to the Study of Plant Biochemistry and Signal Transduction

Autores
Ulloa, Rita Maria; Raices, Marcela; Tellez, Maria Teresa
Año de publicación
2000
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This report describes a one-week laboratory practice for students. An approach to study a Calcium dependent protein kinase (CDPK) involved in signal transduction processes in potato plants, is undertaken. A number of basic biochemical techniques including the partial purification of a protein kinase, protein kinase activity assays, protein determination, SDS-PAGE analysis of phosphorylated proteins and Western blot assays are described.
Fil: Ulloa, Rita Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Raices, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Tellez, Maria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Materia
Plant biochemistry
Solanum tuberosum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/80463
Acceder
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network_name_str CONICET Digital (CONICET)
spelling A One-Week Laboratory Practice: Introducing the Students to the Study of Plant Biochemistry and Signal TransductionUlloa, Rita MariaRaices, MarcelaTellez, Maria TeresaPlant biochemistrySolanum tuberosumhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1This report describes a one-week laboratory practice for students. An approach to study a Calcium dependent protein kinase (CDPK) involved in signal transduction processes in potato plants, is undertaken. A number of basic biochemical techniques including the partial purification of a protein kinase, protein kinase activity assays, protein determination, SDS-PAGE analysis of phosphorylated proteins and Western blot assays are described.Fil: Ulloa, Rita Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Raices, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Tellez, Maria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaAssociation of College and University Biology Educators2000-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/80463Ulloa, Rita Maria; Raices, Marcela; Tellez, Maria Teresa; A One-Week Laboratory Practice: Introducing the Students to the Study of Plant Biochemistry and Signal Transduction; Association of College and University Biology Educators; Bioscene; 26; 2; 12-2000; 3-121539-2422CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.acube.org/wp-content/uploads/2017/11/2000_2.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:18:20Zoai:ri.conicet.gov.ar:11336/80463instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:18:20.49CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A One-Week Laboratory Practice: Introducing the Students to the Study of Plant Biochemistry and Signal Transduction
title A One-Week Laboratory Practice: Introducing the Students to the Study of Plant Biochemistry and Signal Transduction
spellingShingle A One-Week Laboratory Practice: Introducing the Students to the Study of Plant Biochemistry and Signal Transduction
Ulloa, Rita Maria
Plant biochemistry
Solanum tuberosum
title_short A One-Week Laboratory Practice: Introducing the Students to the Study of Plant Biochemistry and Signal Transduction
title_full A One-Week Laboratory Practice: Introducing the Students to the Study of Plant Biochemistry and Signal Transduction
title_fullStr A One-Week Laboratory Practice: Introducing the Students to the Study of Plant Biochemistry and Signal Transduction
title_full_unstemmed A One-Week Laboratory Practice: Introducing the Students to the Study of Plant Biochemistry and Signal Transduction
title_sort A One-Week Laboratory Practice: Introducing the Students to the Study of Plant Biochemistry and Signal Transduction
dc.creator.none.fl_str_mv Ulloa, Rita Maria
Raices, Marcela
Tellez, Maria Teresa
author Ulloa, Rita Maria
author_facet Ulloa, Rita Maria
Raices, Marcela
Tellez, Maria Teresa
author_role author
author2 Raices, Marcela
Tellez, Maria Teresa
author2_role author
author
dc.subject.none.fl_str_mv Plant biochemistry
Solanum tuberosum
topic Plant biochemistry
Solanum tuberosum
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This report describes a one-week laboratory practice for students. An approach to study a Calcium dependent protein kinase (CDPK) involved in signal transduction processes in potato plants, is undertaken. A number of basic biochemical techniques including the partial purification of a protein kinase, protein kinase activity assays, protein determination, SDS-PAGE analysis of phosphorylated proteins and Western blot assays are described.
Fil: Ulloa, Rita Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Raices, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Tellez, Maria Teresa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
description This report describes a one-week laboratory practice for students. An approach to study a Calcium dependent protein kinase (CDPK) involved in signal transduction processes in potato plants, is undertaken. A number of basic biochemical techniques including the partial purification of a protein kinase, protein kinase activity assays, protein determination, SDS-PAGE analysis of phosphorylated proteins and Western blot assays are described.
publishDate 2000
dc.date.none.fl_str_mv 2000-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/80463
Ulloa, Rita Maria; Raices, Marcela; Tellez, Maria Teresa; A One-Week Laboratory Practice: Introducing the Students to the Study of Plant Biochemistry and Signal Transduction; Association of College and University Biology Educators; Bioscene; 26; 2; 12-2000; 3-12
1539-2422
CONICET Digital
CONICET
url http://hdl.handle.net/11336/80463
identifier_str_mv Ulloa, Rita Maria; Raices, Marcela; Tellez, Maria Teresa; A One-Week Laboratory Practice: Introducing the Students to the Study of Plant Biochemistry and Signal Transduction; Association of College and University Biology Educators; Bioscene; 26; 2; 12-2000; 3-12
1539-2422
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.acube.org/wp-content/uploads/2017/11/2000_2.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Association of College and University Biology Educators
publisher.none.fl_str_mv Association of College and University Biology Educators
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782613868511232
score 12.8982525

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