Method development for digoxin determination in biological samples using fluorescence quenching

Autores
Peralta, Cecilia Mariana; Vicario, Ana Laura; Jofre, Maria; Fernandez, Liliana Patricia; Aragón, Leslie Mary; Acosta, Maria Gimena
Año de publicación
2024
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Digoxin (DG) is a cardiac glycoside steroid that is commonly used to cure congestive heart failure and arrhythmias. Several studies support that the effective range of plasma concentration is between 0.5 and 2.0 ng/mL, being it toxic at higher plasma concentration (>2.0 ng/mL). It is also well stablished that the effective plasma drug concentration is close to the toxic, and large individual differences in the effects of the drug have been observed. Therefore, simple, and sensitive procedure is required for strict monitoring of DG in blood level to minimize the risk of toxicity. The main objective of this study was the design of new derivatization strategies that allow DG determination by molecular fluorescence. In this research, a standard solution of DG (Sigma Chemical Co.) was taken up in ethanol (1mg mL-1). Bovine seroalbumine (BSA) was acquired of Lab. Alimentos-UNSL, San Luis, Argentina. All experiments were carried out in a 1 cm quartz cell, using a Shimadzu RF-5301PC spectrofluorimeter, equipped with a Xenon discharge lamp. Several factors that affect the derivatization reaction were studied i.g., type and concentration of the fluorophore (BSA, Rhodamine B, eosyne, quinoleine), pH, ionic strength, organic solvents. Also, the effect of different surfactants was evaluated as improvement fluorescence signal (SDS, Bile salt, HTAB), etc. The spectral behavior observed after derivatization were evaluated to establish the optimal experimental conditions (excitation and emission wavelength: 280 nm and 340 nm (BSA) and 555 nm and 575 nm (RhB), slits: 5/5). The attenuation in the fluorescent signal (quenching effect) with increase DG concentration on BSA and RhB was observed. The studied strategies will be incorporated to schemes of high-performance liquid chromatography associated with fluorescent detection to automate the methodology and allow the determination of DG in biological samples.
Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Vicario, Ana Laura. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina
Fil: Jofre, Maria. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Aragón, Leslie Mary. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Acosta, Maria Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
XLI Reunión Anual de la Sociedad de Biología de Cuyo
Argentina
Sociedad de Biología de Cuyo
Materia
DIGOXIN
FLUORESCENCE
QUENCHING
BIOLOGICAL SAMPLES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/276854
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/276854
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Method development for digoxin determination in biological samples using fluorescence quenchingPeralta, Cecilia MarianaVicario, Ana LauraJofre, MariaFernandez, Liliana PatriciaAragón, Leslie MaryAcosta, Maria GimenaDIGOXINFLUORESCENCEQUENCHINGBIOLOGICAL SAMPLEShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Digoxin (DG) is a cardiac glycoside steroid that is commonly used to cure congestive heart failure and arrhythmias. Several studies support that the effective range of plasma concentration is between 0.5 and 2.0 ng/mL, being it toxic at higher plasma concentration (>2.0 ng/mL). It is also well stablished that the effective plasma drug concentration is close to the toxic, and large individual differences in the effects of the drug have been observed. Therefore, simple, and sensitive procedure is required for strict monitoring of DG in blood level to minimize the risk of toxicity. The main objective of this study was the design of new derivatization strategies that allow DG determination by molecular fluorescence. In this research, a standard solution of DG (Sigma Chemical Co.) was taken up in ethanol (1mg mL-1). Bovine seroalbumine (BSA) was acquired of Lab. Alimentos-UNSL, San Luis, Argentina. All experiments were carried out in a 1 cm quartz cell, using a Shimadzu RF-5301PC spectrofluorimeter, equipped with a Xenon discharge lamp. Several factors that affect the derivatization reaction were studied i.g., type and concentration of the fluorophore (BSA, Rhodamine B, eosyne, quinoleine), pH, ionic strength, organic solvents. Also, the effect of different surfactants was evaluated as improvement fluorescence signal (SDS, Bile salt, HTAB), etc. The spectral behavior observed after derivatization were evaluated to establish the optimal experimental conditions (excitation and emission wavelength: 280 nm and 340 nm (BSA) and 555 nm and 575 nm (RhB), slits: 5/5). The attenuation in the fluorescent signal (quenching effect) with increase DG concentration on BSA and RhB was observed. The studied strategies will be incorporated to schemes of high-performance liquid chromatography associated with fluorescent detection to automate the methodology and allow the determination of DG in biological samples.Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Vicario, Ana Laura. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; ArgentinaFil: Jofre, Maria. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Aragón, Leslie Mary. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Acosta, Maria Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaXLI Reunión Anual de la Sociedad de Biología de CuyoArgentinaSociedad de Biología de CuyoUniversidad Nacional de San Juan2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/276854Method development for digoxin determination in biological samples using fluorescence quenching; XLI Reunión Anual de la Sociedad de Biología de Cuyo; Argentina; 2023; 143-144CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-01-08T13:12:26Zoai:ri.conicet.gov.ar:11336/276854instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-01-08 13:12:26.464CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Method development for digoxin determination in biological samples using fluorescence quenching
title Method development for digoxin determination in biological samples using fluorescence quenching
spellingShingle Method development for digoxin determination in biological samples using fluorescence quenching
Peralta, Cecilia Mariana
DIGOXIN
FLUORESCENCE
QUENCHING
BIOLOGICAL SAMPLES
title_short Method development for digoxin determination in biological samples using fluorescence quenching
title_full Method development for digoxin determination in biological samples using fluorescence quenching
title_fullStr Method development for digoxin determination in biological samples using fluorescence quenching
title_full_unstemmed Method development for digoxin determination in biological samples using fluorescence quenching
title_sort Method development for digoxin determination in biological samples using fluorescence quenching
dc.creator.none.fl_str_mv Peralta, Cecilia Mariana
Vicario, Ana Laura
Jofre, Maria
Fernandez, Liliana Patricia
Aragón, Leslie Mary
Acosta, Maria Gimena
author Peralta, Cecilia Mariana
author_facet Peralta, Cecilia Mariana
Vicario, Ana Laura
Jofre, Maria
Fernandez, Liliana Patricia
Aragón, Leslie Mary
Acosta, Maria Gimena
author_role author
author2 Vicario, Ana Laura
Jofre, Maria
Fernandez, Liliana Patricia
Aragón, Leslie Mary
Acosta, Maria Gimena
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv DIGOXIN
FLUORESCENCE
QUENCHING
BIOLOGICAL SAMPLES
topic DIGOXIN
FLUORESCENCE
QUENCHING
BIOLOGICAL SAMPLES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Digoxin (DG) is a cardiac glycoside steroid that is commonly used to cure congestive heart failure and arrhythmias. Several studies support that the effective range of plasma concentration is between 0.5 and 2.0 ng/mL, being it toxic at higher plasma concentration (>2.0 ng/mL). It is also well stablished that the effective plasma drug concentration is close to the toxic, and large individual differences in the effects of the drug have been observed. Therefore, simple, and sensitive procedure is required for strict monitoring of DG in blood level to minimize the risk of toxicity. The main objective of this study was the design of new derivatization strategies that allow DG determination by molecular fluorescence. In this research, a standard solution of DG (Sigma Chemical Co.) was taken up in ethanol (1mg mL-1). Bovine seroalbumine (BSA) was acquired of Lab. Alimentos-UNSL, San Luis, Argentina. All experiments were carried out in a 1 cm quartz cell, using a Shimadzu RF-5301PC spectrofluorimeter, equipped with a Xenon discharge lamp. Several factors that affect the derivatization reaction were studied i.g., type and concentration of the fluorophore (BSA, Rhodamine B, eosyne, quinoleine), pH, ionic strength, organic solvents. Also, the effect of different surfactants was evaluated as improvement fluorescence signal (SDS, Bile salt, HTAB), etc. The spectral behavior observed after derivatization were evaluated to establish the optimal experimental conditions (excitation and emission wavelength: 280 nm and 340 nm (BSA) and 555 nm and 575 nm (RhB), slits: 5/5). The attenuation in the fluorescent signal (quenching effect) with increase DG concentration on BSA and RhB was observed. The studied strategies will be incorporated to schemes of high-performance liquid chromatography associated with fluorescent detection to automate the methodology and allow the determination of DG in biological samples.
Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Vicario, Ana Laura. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina
Fil: Jofre, Maria. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Aragón, Leslie Mary. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Acosta, Maria Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
XLI Reunión Anual de la Sociedad de Biología de Cuyo
Argentina
Sociedad de Biología de Cuyo
description Digoxin (DG) is a cardiac glycoside steroid that is commonly used to cure congestive heart failure and arrhythmias. Several studies support that the effective range of plasma concentration is between 0.5 and 2.0 ng/mL, being it toxic at higher plasma concentration (>2.0 ng/mL). It is also well stablished that the effective plasma drug concentration is close to the toxic, and large individual differences in the effects of the drug have been observed. Therefore, simple, and sensitive procedure is required for strict monitoring of DG in blood level to minimize the risk of toxicity. The main objective of this study was the design of new derivatization strategies that allow DG determination by molecular fluorescence. In this research, a standard solution of DG (Sigma Chemical Co.) was taken up in ethanol (1mg mL-1). Bovine seroalbumine (BSA) was acquired of Lab. Alimentos-UNSL, San Luis, Argentina. All experiments were carried out in a 1 cm quartz cell, using a Shimadzu RF-5301PC spectrofluorimeter, equipped with a Xenon discharge lamp. Several factors that affect the derivatization reaction were studied i.g., type and concentration of the fluorophore (BSA, Rhodamine B, eosyne, quinoleine), pH, ionic strength, organic solvents. Also, the effect of different surfactants was evaluated as improvement fluorescence signal (SDS, Bile salt, HTAB), etc. The spectral behavior observed after derivatization were evaluated to establish the optimal experimental conditions (excitation and emission wavelength: 280 nm and 340 nm (BSA) and 555 nm and 575 nm (RhB), slits: 5/5). The attenuation in the fluorescent signal (quenching effect) with increase DG concentration on BSA and RhB was observed. The studied strategies will be incorporated to schemes of high-performance liquid chromatography associated with fluorescent detection to automate the methodology and allow the determination of DG in biological samples.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/276854
Method development for digoxin determination in biological samples using fluorescence quenching; XLI Reunión Anual de la Sociedad de Biología de Cuyo; Argentina; 2023; 143-144
CONICET Digital
CONICET
url http://hdl.handle.net/11336/276854
identifier_str_mv Method development for digoxin determination in biological samples using fluorescence quenching; XLI Reunión Anual de la Sociedad de Biología de Cuyo; Argentina; 2023; 143-144
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Universidad Nacional de San Juan
publisher.none.fl_str_mv Universidad Nacional de San Juan
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.747614

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