Molecular Detection Method Developed to Track the Koinobiont Larval Parasitoid Apanteles opuntiarum (Hymenoptera: Braconidae) Imported from Argentina to Control Cactoblastis cactor...

Autores
Srivastava, Mrittunjai; Srivastava, Pratibha; Karan, Ratna; Jeyaprakash, Ayyamperumal; Whilby, Leroy; Rohrig, Eric; Howe, Amy C.; Hight, Stephen D.; Varone, Laura
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Apanteles opuntiarum Martínez & Berta (Hymenoptera: Braconidae) is a native natural enemy of the cactus moth, Cactoblastis cactorum Berg (Lepidoptera: Pyralidae) in Argentina, where the 2 species are believed to have co-evolved. Cactoblastis cactorum is an established invasive pest in the US that is rapidly spreading throughout the southeast. Apanteles opuntiarum was imported from Argentina, and reared at the Division of Plant Industry containment facility in Gainesville, Florida, for study as a possible biocontrol agent for release in the US to control C. cactorum. A DNA barcode was developed to enable the identification of the reared parasitoid population. The mitochondrial cytochrome oxidase I (COI) gene of the A. opuntiarum reared in Florida containment was found to be identical to its Argentine founders, but distinctly different from the COI sequences of all other reported Apanteles species in the NCBI (National Center for Biotechnology Information) GenBank. Additionally, the AoF1 and AoR1 primer pair developed in this study specifically amplified the COI gene of A. opuntiarum, but did not amplify the COI gene of the host C. cactorum. Therefore, the COI gene fragment identified in this study has the potential to be used as a DNA barcode specific to A. opuntiarum that can aid in tracking and identifying this parasitoid inside hosts.
Apanteles opuntiarum Martínez & Berta (Hymenoptera: Braconidae) es un enemigo natural de la polilla de la tuna Cactoblastis cactorum Berg (Lepidoptera: Pyralidae) en Argentina, su rango nativo, donde han co-evolucionado. Cactoblastis cactorum es una especie invasora establecida en Estados Unidos, que se está dispersando rápidamente hacia el sudeste de este país. Apanteles opuntiarum fue importado desde Argentina y es criado en la cuarentena de Gainesville, Florida (Division of Plant Industry), donde está siendo evaluado como posible agente de control de C. cactorum, para ser liberado en Estados Unidos. Se desarrolló un código de barras de ADN para permitir la identificación de la población de parasitoides criada. Se encontró que el gen de la citocromo oxidasa mitocondrial I (COI) de los A. opuntiarum criados en Florida fue idéntico al de sus fundadores argentinos, y claramente diferente de las secuencias de COI de todas las demás especies de Apanteles reportados en el GenBank del NCBI (Centro Nacional de información sobre biotecnología). Además, el par “primer” AoF1 y AoR1 desarrollado en este estudio amplificó específicamente el gen COI de A. opuntiarum, y no amplificó el gen de la COI del hospedador C. cactorum. Por lo tanto, el fragmento del gen COI identificado en este estudio tiene el potencial para ser utilizados como un código de barras de ADN específico para A. opuntiarum que puede ayudar en el seguimiento y la identificación de este parasitoide dentro de los hospedadores
Fil: Srivastava, Mrittunjai. Florida Department of Agriculture and Consumer Services; Estados Unidos
Fil: Srivastava, Pratibha. Florida Department of Agriculture and Consumer Services; Estados Unidos
Fil: Karan, Ratna. Florida Department of Agriculture and Consumer Services; Estados Unidos
Fil: Jeyaprakash, Ayyamperumal. Florida Department of Agriculture and Consumer Services; Estados Unidos
Fil: Whilby, Leroy. Florida Department of Agriculture and Consumer Services; Estados Unidos
Fil: Rohrig, Eric. Florida Department of Agriculture and Consumer Services; Estados Unidos
Fil: Howe, Amy C.. Florida Department of Agriculture and Consumer Services; Estados Unidos
Fil: Hight, Stephen D.. United States Department of Agriculture. Agriculture Research Service; Estados Unidos
Fil: Varone, Laura. Fundación para el Estudio de Especies Invasivas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
BIOLOGICAL CONTROL
COI GENE
DNA BARCODE
HOST RANGE TESTS
OPUNTIA
PARASITOIDS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/130282
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/130282
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular Detection Method Developed to Track the Koinobiont Larval Parasitoid Apanteles opuntiarum (Hymenoptera: Braconidae) Imported from Argentina to Control Cactoblastis cactorum (Lepidoptera: Pyralidae)Srivastava, MrittunjaiSrivastava, PratibhaKaran, RatnaJeyaprakash, AyyamperumalWhilby, LeroyRohrig, EricHowe, Amy C.Hight, Stephen D.Varone, LauraBIOLOGICAL CONTROLCOI GENEDNA BARCODEHOST RANGE TESTSOPUNTIAPARASITOIDShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Apanteles opuntiarum Martínez & Berta (Hymenoptera: Braconidae) is a native natural enemy of the cactus moth, Cactoblastis cactorum Berg (Lepidoptera: Pyralidae) in Argentina, where the 2 species are believed to have co-evolved. Cactoblastis cactorum is an established invasive pest in the US that is rapidly spreading throughout the southeast. Apanteles opuntiarum was imported from Argentina, and reared at the Division of Plant Industry containment facility in Gainesville, Florida, for study as a possible biocontrol agent for release in the US to control C. cactorum. A DNA barcode was developed to enable the identification of the reared parasitoid population. The mitochondrial cytochrome oxidase I (COI) gene of the A. opuntiarum reared in Florida containment was found to be identical to its Argentine founders, but distinctly different from the COI sequences of all other reported Apanteles species in the NCBI (National Center for Biotechnology Information) GenBank. Additionally, the AoF1 and AoR1 primer pair developed in this study specifically amplified the COI gene of A. opuntiarum, but did not amplify the COI gene of the host C. cactorum. Therefore, the COI gene fragment identified in this study has the potential to be used as a DNA barcode specific to A. opuntiarum that can aid in tracking and identifying this parasitoid inside hosts.Apanteles opuntiarum Martínez & Berta (Hymenoptera: Braconidae) es un enemigo natural de la polilla de la tuna Cactoblastis cactorum Berg (Lepidoptera: Pyralidae) en Argentina, su rango nativo, donde han co-evolucionado. Cactoblastis cactorum es una especie invasora establecida en Estados Unidos, que se está dispersando rápidamente hacia el sudeste de este país. Apanteles opuntiarum fue importado desde Argentina y es criado en la cuarentena de Gainesville, Florida (Division of Plant Industry), donde está siendo evaluado como posible agente de control de C. cactorum, para ser liberado en Estados Unidos. Se desarrolló un código de barras de ADN para permitir la identificación de la población de parasitoides criada. Se encontró que el gen de la citocromo oxidasa mitocondrial I (COI) de los A. opuntiarum criados en Florida fue idéntico al de sus fundadores argentinos, y claramente diferente de las secuencias de COI de todas las demás especies de Apanteles reportados en el GenBank del NCBI (Centro Nacional de información sobre biotecnología). Además, el par “primer” AoF1 y AoR1 desarrollado en este estudio amplificó específicamente el gen COI de A. opuntiarum, y no amplificó el gen de la COI del hospedador C. cactorum. Por lo tanto, el fragmento del gen COI identificado en este estudio tiene el potencial para ser utilizados como un código de barras de ADN específico para A. opuntiarum que puede ayudar en el seguimiento y la identificación de este parasitoide dentro de los hospedadoresFil: Srivastava, Mrittunjai. Florida Department of Agriculture and Consumer Services; Estados UnidosFil: Srivastava, Pratibha. Florida Department of Agriculture and Consumer Services; Estados UnidosFil: Karan, Ratna. Florida Department of Agriculture and Consumer Services; Estados UnidosFil: Jeyaprakash, Ayyamperumal. Florida Department of Agriculture and Consumer Services; Estados UnidosFil: Whilby, Leroy. Florida Department of Agriculture and Consumer Services; Estados UnidosFil: Rohrig, Eric. Florida Department of Agriculture and Consumer Services; Estados UnidosFil: Howe, Amy C.. Florida Department of Agriculture and Consumer Services; Estados UnidosFil: Hight, Stephen D.. United States Department of Agriculture. Agriculture Research Service; Estados UnidosFil: Varone, Laura. Fundación para el Estudio de Especies Invasivas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFlorida Entomological Society2019-06-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/130282Srivastava, Mrittunjai; Srivastava, Pratibha; Karan, Ratna; Jeyaprakash, Ayyamperumal; Whilby, Leroy; et al.; Molecular Detection Method Developed to Track the Koinobiont Larval Parasitoid Apanteles opuntiarum (Hymenoptera: Braconidae) Imported from Argentina to Control Cactoblastis cactorum (Lepidoptera: Pyralidae); Florida Entomological Society; Florida Entomologist; 102; 2; 14-6-2019; 329-3350015-4040CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1653/024.102.0207info:eu-repo/semantics/altIdentifier/url/https://bioone.org/journals/florida-entomologist/volume-102/issue-2/024.102.0207/Molecular-Detection-Method-Developed-to-Track-the-Koinobiont-Larval-Parasitoid/10.1653/024.102.0207.fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:55Zoai:ri.conicet.gov.ar:11336/130282instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:56.166CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular Detection Method Developed to Track the Koinobiont Larval Parasitoid Apanteles opuntiarum (Hymenoptera: Braconidae) Imported from Argentina to Control Cactoblastis cactorum (Lepidoptera: Pyralidae)
title Molecular Detection Method Developed to Track the Koinobiont Larval Parasitoid Apanteles opuntiarum (Hymenoptera: Braconidae) Imported from Argentina to Control Cactoblastis cactorum (Lepidoptera: Pyralidae)
spellingShingle Molecular Detection Method Developed to Track the Koinobiont Larval Parasitoid Apanteles opuntiarum (Hymenoptera: Braconidae) Imported from Argentina to Control Cactoblastis cactorum (Lepidoptera: Pyralidae)
Srivastava, Mrittunjai
BIOLOGICAL CONTROL
COI GENE
DNA BARCODE
HOST RANGE TESTS
OPUNTIA
PARASITOIDS
title_short Molecular Detection Method Developed to Track the Koinobiont Larval Parasitoid Apanteles opuntiarum (Hymenoptera: Braconidae) Imported from Argentina to Control Cactoblastis cactorum (Lepidoptera: Pyralidae)
title_full Molecular Detection Method Developed to Track the Koinobiont Larval Parasitoid Apanteles opuntiarum (Hymenoptera: Braconidae) Imported from Argentina to Control Cactoblastis cactorum (Lepidoptera: Pyralidae)
title_fullStr Molecular Detection Method Developed to Track the Koinobiont Larval Parasitoid Apanteles opuntiarum (Hymenoptera: Braconidae) Imported from Argentina to Control Cactoblastis cactorum (Lepidoptera: Pyralidae)
title_full_unstemmed Molecular Detection Method Developed to Track the Koinobiont Larval Parasitoid Apanteles opuntiarum (Hymenoptera: Braconidae) Imported from Argentina to Control Cactoblastis cactorum (Lepidoptera: Pyralidae)
title_sort Molecular Detection Method Developed to Track the Koinobiont Larval Parasitoid Apanteles opuntiarum (Hymenoptera: Braconidae) Imported from Argentina to Control Cactoblastis cactorum (Lepidoptera: Pyralidae)
dc.creator.none.fl_str_mv Srivastava, Mrittunjai
Srivastava, Pratibha
Karan, Ratna
Jeyaprakash, Ayyamperumal
Whilby, Leroy
Rohrig, Eric
Howe, Amy C.
Hight, Stephen D.
Varone, Laura
author Srivastava, Mrittunjai
author_facet Srivastava, Mrittunjai
Srivastava, Pratibha
Karan, Ratna
Jeyaprakash, Ayyamperumal
Whilby, Leroy
Rohrig, Eric
Howe, Amy C.
Hight, Stephen D.
Varone, Laura
author_role author
author2 Srivastava, Pratibha
Karan, Ratna
Jeyaprakash, Ayyamperumal
Whilby, Leroy
Rohrig, Eric
Howe, Amy C.
Hight, Stephen D.
Varone, Laura
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOLOGICAL CONTROL
COI GENE
DNA BARCODE
HOST RANGE TESTS
OPUNTIA
PARASITOIDS
topic BIOLOGICAL CONTROL
COI GENE
DNA BARCODE
HOST RANGE TESTS
OPUNTIA
PARASITOIDS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Apanteles opuntiarum Martínez & Berta (Hymenoptera: Braconidae) is a native natural enemy of the cactus moth, Cactoblastis cactorum Berg (Lepidoptera: Pyralidae) in Argentina, where the 2 species are believed to have co-evolved. Cactoblastis cactorum is an established invasive pest in the US that is rapidly spreading throughout the southeast. Apanteles opuntiarum was imported from Argentina, and reared at the Division of Plant Industry containment facility in Gainesville, Florida, for study as a possible biocontrol agent for release in the US to control C. cactorum. A DNA barcode was developed to enable the identification of the reared parasitoid population. The mitochondrial cytochrome oxidase I (COI) gene of the A. opuntiarum reared in Florida containment was found to be identical to its Argentine founders, but distinctly different from the COI sequences of all other reported Apanteles species in the NCBI (National Center for Biotechnology Information) GenBank. Additionally, the AoF1 and AoR1 primer pair developed in this study specifically amplified the COI gene of A. opuntiarum, but did not amplify the COI gene of the host C. cactorum. Therefore, the COI gene fragment identified in this study has the potential to be used as a DNA barcode specific to A. opuntiarum that can aid in tracking and identifying this parasitoid inside hosts.
Apanteles opuntiarum Martínez & Berta (Hymenoptera: Braconidae) es un enemigo natural de la polilla de la tuna Cactoblastis cactorum Berg (Lepidoptera: Pyralidae) en Argentina, su rango nativo, donde han co-evolucionado. Cactoblastis cactorum es una especie invasora establecida en Estados Unidos, que se está dispersando rápidamente hacia el sudeste de este país. Apanteles opuntiarum fue importado desde Argentina y es criado en la cuarentena de Gainesville, Florida (Division of Plant Industry), donde está siendo evaluado como posible agente de control de C. cactorum, para ser liberado en Estados Unidos. Se desarrolló un código de barras de ADN para permitir la identificación de la población de parasitoides criada. Se encontró que el gen de la citocromo oxidasa mitocondrial I (COI) de los A. opuntiarum criados en Florida fue idéntico al de sus fundadores argentinos, y claramente diferente de las secuencias de COI de todas las demás especies de Apanteles reportados en el GenBank del NCBI (Centro Nacional de información sobre biotecnología). Además, el par “primer” AoF1 y AoR1 desarrollado en este estudio amplificó específicamente el gen COI de A. opuntiarum, y no amplificó el gen de la COI del hospedador C. cactorum. Por lo tanto, el fragmento del gen COI identificado en este estudio tiene el potencial para ser utilizados como un código de barras de ADN específico para A. opuntiarum que puede ayudar en el seguimiento y la identificación de este parasitoide dentro de los hospedadores
Fil: Srivastava, Mrittunjai. Florida Department of Agriculture and Consumer Services; Estados Unidos
Fil: Srivastava, Pratibha. Florida Department of Agriculture and Consumer Services; Estados Unidos
Fil: Karan, Ratna. Florida Department of Agriculture and Consumer Services; Estados Unidos
Fil: Jeyaprakash, Ayyamperumal. Florida Department of Agriculture and Consumer Services; Estados Unidos
Fil: Whilby, Leroy. Florida Department of Agriculture and Consumer Services; Estados Unidos
Fil: Rohrig, Eric. Florida Department of Agriculture and Consumer Services; Estados Unidos
Fil: Howe, Amy C.. Florida Department of Agriculture and Consumer Services; Estados Unidos
Fil: Hight, Stephen D.. United States Department of Agriculture. Agriculture Research Service; Estados Unidos
Fil: Varone, Laura. Fundación para el Estudio de Especies Invasivas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Apanteles opuntiarum Martínez & Berta (Hymenoptera: Braconidae) is a native natural enemy of the cactus moth, Cactoblastis cactorum Berg (Lepidoptera: Pyralidae) in Argentina, where the 2 species are believed to have co-evolved. Cactoblastis cactorum is an established invasive pest in the US that is rapidly spreading throughout the southeast. Apanteles opuntiarum was imported from Argentina, and reared at the Division of Plant Industry containment facility in Gainesville, Florida, for study as a possible biocontrol agent for release in the US to control C. cactorum. A DNA barcode was developed to enable the identification of the reared parasitoid population. The mitochondrial cytochrome oxidase I (COI) gene of the A. opuntiarum reared in Florida containment was found to be identical to its Argentine founders, but distinctly different from the COI sequences of all other reported Apanteles species in the NCBI (National Center for Biotechnology Information) GenBank. Additionally, the AoF1 and AoR1 primer pair developed in this study specifically amplified the COI gene of A. opuntiarum, but did not amplify the COI gene of the host C. cactorum. Therefore, the COI gene fragment identified in this study has the potential to be used as a DNA barcode specific to A. opuntiarum that can aid in tracking and identifying this parasitoid inside hosts.
publishDate 2019
dc.date.none.fl_str_mv 2019-06-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/130282
Srivastava, Mrittunjai; Srivastava, Pratibha; Karan, Ratna; Jeyaprakash, Ayyamperumal; Whilby, Leroy; et al.; Molecular Detection Method Developed to Track the Koinobiont Larval Parasitoid Apanteles opuntiarum (Hymenoptera: Braconidae) Imported from Argentina to Control Cactoblastis cactorum (Lepidoptera: Pyralidae); Florida Entomological Society; Florida Entomologist; 102; 2; 14-6-2019; 329-335
0015-4040
CONICET Digital
CONICET
url http://hdl.handle.net/11336/130282
identifier_str_mv Srivastava, Mrittunjai; Srivastava, Pratibha; Karan, Ratna; Jeyaprakash, Ayyamperumal; Whilby, Leroy; et al.; Molecular Detection Method Developed to Track the Koinobiont Larval Parasitoid Apanteles opuntiarum (Hymenoptera: Braconidae) Imported from Argentina to Control Cactoblastis cactorum (Lepidoptera: Pyralidae); Florida Entomological Society; Florida Entomologist; 102; 2; 14-6-2019; 329-335
0015-4040
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1653/024.102.0207
info:eu-repo/semantics/altIdentifier/url/https://bioone.org/journals/florida-entomologist/volume-102/issue-2/024.102.0207/Molecular-Detection-Method-Developed-to-Track-the-Koinobiont-Larval-Parasitoid/10.1653/024.102.0207.full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Florida Entomological Society
publisher.none.fl_str_mv Florida Entomological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.885934

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