Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair?

Autores
Padula, Gisel; Gonzalez, Horacio F.; Varea, Ana; Seoane, Analia Isabel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Protein-energy malnutrition (PEM) is originated by a cellular imbalance between nutrient/energy supply and body’s demand. Induction of genetic damage by PEM was reported. The purpose of this study was to determine the genetic effect of the in vitro zinc sulfate (ZnSO4) supplementation of cultured peripheral blood lymphocytes from children with PEM. Twenty-four samples from 12 children were analyzed. Anthropometric and biochemical diagnosis was made. For the anthropometric assessment, height-for-age index, weight-for-age index, and weight-for-height index were calculated (WHO, 2005). Micronutrient status was evaluated. A survey for assessed previous exposure to potentially genotoxic agents was applied. Results were statistically evaluated using paired sample t test and χ2 test. Each sample was fractionated and cultured in two separate flasks to performed two treatments. One was added with 180 μg/dl of ZnSO4 (PEMs/ZnSO4) and the other remains non-supplemented (PEMs). Cytotoxic effects and chromosomal damage were assessed using the cytokinesis-block micronucleus assay (CBMN). All participants have at least one type of malnutrition and none have anemia, nor iron, folate, vitamin A, and zinc deficiency. All PEMs/ZnSO4 samples have a significant reduction in the micronucleus (MNi) frequency compared with PEMs (t=6.25685; p<0.001). Nuclear division index (NDI) increase in PEMs/ZnSO4 (t=−17.4226; p<0.001). Nucleoplasmic bridge (NPBs) frequency was four times smaller in PEMs/ZnSO4 (χ2 =40.82; p<0.001). No nuclear buds (NBuds) were observed. Cytotoxic effects and chromosomal damage observed in children suffering from PEM can be repaired in vitro with zinc sulfate supplementation.
Fil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Gonzalez, Horacio F.. Hospital de Niños de La Plata; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Varea, Ana. Hospital de Niños de La Plata; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Materia
Protein-Energy Malnutrition
Chromosomal Damage
Zinc Sulfate
Children
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/25032

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network_name_str CONICET Digital (CONICET)
spelling Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair?Padula, GiselGonzalez, Horacio F.Varea, AnaSeoane, Analia IsabelProtein-Energy MalnutritionChromosomal DamageZinc SulfateChildrenhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Protein-energy malnutrition (PEM) is originated by a cellular imbalance between nutrient/energy supply and body’s demand. Induction of genetic damage by PEM was reported. The purpose of this study was to determine the genetic effect of the in vitro zinc sulfate (ZnSO4) supplementation of cultured peripheral blood lymphocytes from children with PEM. Twenty-four samples from 12 children were analyzed. Anthropometric and biochemical diagnosis was made. For the anthropometric assessment, height-for-age index, weight-for-age index, and weight-for-height index were calculated (WHO, 2005). Micronutrient status was evaluated. A survey for assessed previous exposure to potentially genotoxic agents was applied. Results were statistically evaluated using paired sample t test and χ2 test. Each sample was fractionated and cultured in two separate flasks to performed two treatments. One was added with 180 μg/dl of ZnSO4 (PEMs/ZnSO4) and the other remains non-supplemented (PEMs). Cytotoxic effects and chromosomal damage were assessed using the cytokinesis-block micronucleus assay (CBMN). All participants have at least one type of malnutrition and none have anemia, nor iron, folate, vitamin A, and zinc deficiency. All PEMs/ZnSO4 samples have a significant reduction in the micronucleus (MNi) frequency compared with PEMs (t=6.25685; p<0.001). Nuclear division index (NDI) increase in PEMs/ZnSO4 (t=−17.4226; p<0.001). Nucleoplasmic bridge (NPBs) frequency was four times smaller in PEMs/ZnSO4 (χ2 =40.82; p<0.001). No nuclear buds (NBuds) were observed. Cytotoxic effects and chromosomal damage observed in children suffering from PEM can be repaired in vitro with zinc sulfate supplementation.Fil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Gonzalez, Horacio F.. Hospital de Niños de La Plata; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Varea, Ana. Hospital de Niños de La Plata; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaHumana Press2014-09-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25032Padula, Gisel; Gonzalez, Horacio F.; Varea, Ana; Seoane, Analia Isabel; Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair?; Humana Press; Biological Trace Element Research; 162; 1-3; 28-9-2014; 64-710163-4984CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s12011-014-0109-2info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12011-014-0109-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:59Zoai:ri.conicet.gov.ar:11336/25032instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:59.881CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair?
title Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair?
spellingShingle Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair?
Padula, Gisel
Protein-Energy Malnutrition
Chromosomal Damage
Zinc Sulfate
Children
title_short Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair?
title_full Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair?
title_fullStr Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair?
title_full_unstemmed Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair?
title_sort Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair?
dc.creator.none.fl_str_mv Padula, Gisel
Gonzalez, Horacio F.
Varea, Ana
Seoane, Analia Isabel
author Padula, Gisel
author_facet Padula, Gisel
Gonzalez, Horacio F.
Varea, Ana
Seoane, Analia Isabel
author_role author
author2 Gonzalez, Horacio F.
Varea, Ana
Seoane, Analia Isabel
author2_role author
author
author
dc.subject.none.fl_str_mv Protein-Energy Malnutrition
Chromosomal Damage
Zinc Sulfate
Children
topic Protein-Energy Malnutrition
Chromosomal Damage
Zinc Sulfate
Children
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Protein-energy malnutrition (PEM) is originated by a cellular imbalance between nutrient/energy supply and body’s demand. Induction of genetic damage by PEM was reported. The purpose of this study was to determine the genetic effect of the in vitro zinc sulfate (ZnSO4) supplementation of cultured peripheral blood lymphocytes from children with PEM. Twenty-four samples from 12 children were analyzed. Anthropometric and biochemical diagnosis was made. For the anthropometric assessment, height-for-age index, weight-for-age index, and weight-for-height index were calculated (WHO, 2005). Micronutrient status was evaluated. A survey for assessed previous exposure to potentially genotoxic agents was applied. Results were statistically evaluated using paired sample t test and χ2 test. Each sample was fractionated and cultured in two separate flasks to performed two treatments. One was added with 180 μg/dl of ZnSO4 (PEMs/ZnSO4) and the other remains non-supplemented (PEMs). Cytotoxic effects and chromosomal damage were assessed using the cytokinesis-block micronucleus assay (CBMN). All participants have at least one type of malnutrition and none have anemia, nor iron, folate, vitamin A, and zinc deficiency. All PEMs/ZnSO4 samples have a significant reduction in the micronucleus (MNi) frequency compared with PEMs (t=6.25685; p<0.001). Nuclear division index (NDI) increase in PEMs/ZnSO4 (t=−17.4226; p<0.001). Nucleoplasmic bridge (NPBs) frequency was four times smaller in PEMs/ZnSO4 (χ2 =40.82; p<0.001). No nuclear buds (NBuds) were observed. Cytotoxic effects and chromosomal damage observed in children suffering from PEM can be repaired in vitro with zinc sulfate supplementation.
Fil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Gonzalez, Horacio F.. Hospital de Niños de La Plata; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Varea, Ana. Hospital de Niños de La Plata; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
description Protein-energy malnutrition (PEM) is originated by a cellular imbalance between nutrient/energy supply and body’s demand. Induction of genetic damage by PEM was reported. The purpose of this study was to determine the genetic effect of the in vitro zinc sulfate (ZnSO4) supplementation of cultured peripheral blood lymphocytes from children with PEM. Twenty-four samples from 12 children were analyzed. Anthropometric and biochemical diagnosis was made. For the anthropometric assessment, height-for-age index, weight-for-age index, and weight-for-height index were calculated (WHO, 2005). Micronutrient status was evaluated. A survey for assessed previous exposure to potentially genotoxic agents was applied. Results were statistically evaluated using paired sample t test and χ2 test. Each sample was fractionated and cultured in two separate flasks to performed two treatments. One was added with 180 μg/dl of ZnSO4 (PEMs/ZnSO4) and the other remains non-supplemented (PEMs). Cytotoxic effects and chromosomal damage were assessed using the cytokinesis-block micronucleus assay (CBMN). All participants have at least one type of malnutrition and none have anemia, nor iron, folate, vitamin A, and zinc deficiency. All PEMs/ZnSO4 samples have a significant reduction in the micronucleus (MNi) frequency compared with PEMs (t=6.25685; p<0.001). Nuclear division index (NDI) increase in PEMs/ZnSO4 (t=−17.4226; p<0.001). Nucleoplasmic bridge (NPBs) frequency was four times smaller in PEMs/ZnSO4 (χ2 =40.82; p<0.001). No nuclear buds (NBuds) were observed. Cytotoxic effects and chromosomal damage observed in children suffering from PEM can be repaired in vitro with zinc sulfate supplementation.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-28
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/25032
Padula, Gisel; Gonzalez, Horacio F.; Varea, Ana; Seoane, Analia Isabel; Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair?; Humana Press; Biological Trace Element Research; 162; 1-3; 28-9-2014; 64-71
0163-4984
CONICET Digital
CONICET
url http://hdl.handle.net/11336/25032
identifier_str_mv Padula, Gisel; Gonzalez, Horacio F.; Varea, Ana; Seoane, Analia Isabel; Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair?; Humana Press; Biological Trace Element Research; 162; 1-3; 28-9-2014; 64-71
0163-4984
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s12011-014-0109-2
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12011-014-0109-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Humana Press
publisher.none.fl_str_mv Humana Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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