Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair?
- Autores
- Padula, Gisel; Gonzalez, Horacio F.; Varea, Ana; Seoane, Analia Isabel
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Protein-energy malnutrition (PEM) is originated by a cellular imbalance between nutrient/energy supply and body’s demand. Induction of genetic damage by PEM was reported. The purpose of this study was to determine the genetic effect of the in vitro zinc sulfate (ZnSO4) supplementation of cultured peripheral blood lymphocytes from children with PEM. Twenty-four samples from 12 children were analyzed. Anthropometric and biochemical diagnosis was made. For the anthropometric assessment, height-for-age index, weight-for-age index, and weight-for-height index were calculated (WHO, 2005). Micronutrient status was evaluated. A survey for assessed previous exposure to potentially genotoxic agents was applied. Results were statistically evaluated using paired sample t test and χ2 test. Each sample was fractionated and cultured in two separate flasks to performed two treatments. One was added with 180 μg/dl of ZnSO4 (PEMs/ZnSO4) and the other remains non-supplemented (PEMs). Cytotoxic effects and chromosomal damage were assessed using the cytokinesis-block micronucleus assay (CBMN). All participants have at least one type of malnutrition and none have anemia, nor iron, folate, vitamin A, and zinc deficiency. All PEMs/ZnSO4 samples have a significant reduction in the micronucleus (MNi) frequency compared with PEMs (t=6.25685; p<0.001). Nuclear division index (NDI) increase in PEMs/ZnSO4 (t=−17.4226; p<0.001). Nucleoplasmic bridge (NPBs) frequency was four times smaller in PEMs/ZnSO4 (χ2 =40.82; p<0.001). No nuclear buds (NBuds) were observed. Cytotoxic effects and chromosomal damage observed in children suffering from PEM can be repaired in vitro with zinc sulfate supplementation.
Fil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Gonzalez, Horacio F.. Hospital de Niños de La Plata; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Varea, Ana. Hospital de Niños de La Plata; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina - Materia
-
Protein-Energy Malnutrition
Chromosomal Damage
Zinc Sulfate
Children - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/25032
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Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair?Padula, GiselGonzalez, Horacio F.Varea, AnaSeoane, Analia IsabelProtein-Energy MalnutritionChromosomal DamageZinc SulfateChildrenhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Protein-energy malnutrition (PEM) is originated by a cellular imbalance between nutrient/energy supply and body’s demand. Induction of genetic damage by PEM was reported. The purpose of this study was to determine the genetic effect of the in vitro zinc sulfate (ZnSO4) supplementation of cultured peripheral blood lymphocytes from children with PEM. Twenty-four samples from 12 children were analyzed. Anthropometric and biochemical diagnosis was made. For the anthropometric assessment, height-for-age index, weight-for-age index, and weight-for-height index were calculated (WHO, 2005). Micronutrient status was evaluated. A survey for assessed previous exposure to potentially genotoxic agents was applied. Results were statistically evaluated using paired sample t test and χ2 test. Each sample was fractionated and cultured in two separate flasks to performed two treatments. One was added with 180 μg/dl of ZnSO4 (PEMs/ZnSO4) and the other remains non-supplemented (PEMs). Cytotoxic effects and chromosomal damage were assessed using the cytokinesis-block micronucleus assay (CBMN). All participants have at least one type of malnutrition and none have anemia, nor iron, folate, vitamin A, and zinc deficiency. All PEMs/ZnSO4 samples have a significant reduction in the micronucleus (MNi) frequency compared with PEMs (t=6.25685; p<0.001). Nuclear division index (NDI) increase in PEMs/ZnSO4 (t=−17.4226; p<0.001). Nucleoplasmic bridge (NPBs) frequency was four times smaller in PEMs/ZnSO4 (χ2 =40.82; p<0.001). No nuclear buds (NBuds) were observed. Cytotoxic effects and chromosomal damage observed in children suffering from PEM can be repaired in vitro with zinc sulfate supplementation.Fil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Gonzalez, Horacio F.. Hospital de Niños de La Plata; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Varea, Ana. Hospital de Niños de La Plata; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaHumana Press2014-09-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/25032Padula, Gisel; Gonzalez, Horacio F.; Varea, Ana; Seoane, Analia Isabel; Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair?; Humana Press; Biological Trace Element Research; 162; 1-3; 28-9-2014; 64-710163-4984CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s12011-014-0109-2info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12011-014-0109-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:59Zoai:ri.conicet.gov.ar:11336/25032instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:59.881CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair? |
title |
Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair? |
spellingShingle |
Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair? Padula, Gisel Protein-Energy Malnutrition Chromosomal Damage Zinc Sulfate Children |
title_short |
Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair? |
title_full |
Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair? |
title_fullStr |
Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair? |
title_full_unstemmed |
Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair? |
title_sort |
Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair? |
dc.creator.none.fl_str_mv |
Padula, Gisel Gonzalez, Horacio F. Varea, Ana Seoane, Analia Isabel |
author |
Padula, Gisel |
author_facet |
Padula, Gisel Gonzalez, Horacio F. Varea, Ana Seoane, Analia Isabel |
author_role |
author |
author2 |
Gonzalez, Horacio F. Varea, Ana Seoane, Analia Isabel |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Protein-Energy Malnutrition Chromosomal Damage Zinc Sulfate Children |
topic |
Protein-Energy Malnutrition Chromosomal Damage Zinc Sulfate Children |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Protein-energy malnutrition (PEM) is originated by a cellular imbalance between nutrient/energy supply and body’s demand. Induction of genetic damage by PEM was reported. The purpose of this study was to determine the genetic effect of the in vitro zinc sulfate (ZnSO4) supplementation of cultured peripheral blood lymphocytes from children with PEM. Twenty-four samples from 12 children were analyzed. Anthropometric and biochemical diagnosis was made. For the anthropometric assessment, height-for-age index, weight-for-age index, and weight-for-height index were calculated (WHO, 2005). Micronutrient status was evaluated. A survey for assessed previous exposure to potentially genotoxic agents was applied. Results were statistically evaluated using paired sample t test and χ2 test. Each sample was fractionated and cultured in two separate flasks to performed two treatments. One was added with 180 μg/dl of ZnSO4 (PEMs/ZnSO4) and the other remains non-supplemented (PEMs). Cytotoxic effects and chromosomal damage were assessed using the cytokinesis-block micronucleus assay (CBMN). All participants have at least one type of malnutrition and none have anemia, nor iron, folate, vitamin A, and zinc deficiency. All PEMs/ZnSO4 samples have a significant reduction in the micronucleus (MNi) frequency compared with PEMs (t=6.25685; p<0.001). Nuclear division index (NDI) increase in PEMs/ZnSO4 (t=−17.4226; p<0.001). Nucleoplasmic bridge (NPBs) frequency was four times smaller in PEMs/ZnSO4 (χ2 =40.82; p<0.001). No nuclear buds (NBuds) were observed. Cytotoxic effects and chromosomal damage observed in children suffering from PEM can be repaired in vitro with zinc sulfate supplementation. Fil: Padula, Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina Fil: Gonzalez, Horacio F.. Hospital de Niños de La Plata; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina Fil: Varea, Ana. Hospital de Niños de La Plata; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina Fil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina |
description |
Protein-energy malnutrition (PEM) is originated by a cellular imbalance between nutrient/energy supply and body’s demand. Induction of genetic damage by PEM was reported. The purpose of this study was to determine the genetic effect of the in vitro zinc sulfate (ZnSO4) supplementation of cultured peripheral blood lymphocytes from children with PEM. Twenty-four samples from 12 children were analyzed. Anthropometric and biochemical diagnosis was made. For the anthropometric assessment, height-for-age index, weight-for-age index, and weight-for-height index were calculated (WHO, 2005). Micronutrient status was evaluated. A survey for assessed previous exposure to potentially genotoxic agents was applied. Results were statistically evaluated using paired sample t test and χ2 test. Each sample was fractionated and cultured in two separate flasks to performed two treatments. One was added with 180 μg/dl of ZnSO4 (PEMs/ZnSO4) and the other remains non-supplemented (PEMs). Cytotoxic effects and chromosomal damage were assessed using the cytokinesis-block micronucleus assay (CBMN). All participants have at least one type of malnutrition and none have anemia, nor iron, folate, vitamin A, and zinc deficiency. All PEMs/ZnSO4 samples have a significant reduction in the micronucleus (MNi) frequency compared with PEMs (t=6.25685; p<0.001). Nuclear division index (NDI) increase in PEMs/ZnSO4 (t=−17.4226; p<0.001). Nucleoplasmic bridge (NPBs) frequency was four times smaller in PEMs/ZnSO4 (χ2 =40.82; p<0.001). No nuclear buds (NBuds) were observed. Cytotoxic effects and chromosomal damage observed in children suffering from PEM can be repaired in vitro with zinc sulfate supplementation. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-28 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/25032 Padula, Gisel; Gonzalez, Horacio F.; Varea, Ana; Seoane, Analia Isabel; Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair?; Humana Press; Biological Trace Element Research; 162; 1-3; 28-9-2014; 64-71 0163-4984 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/25032 |
identifier_str_mv |
Padula, Gisel; Gonzalez, Horacio F.; Varea, Ana; Seoane, Analia Isabel; Protein Energy-Malnutrition: Does the In Vitro Zinc Sulfate Supplementation Improve Chromosomal Damage Repair?; Humana Press; Biological Trace Element Research; 162; 1-3; 28-9-2014; 64-71 0163-4984 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12011-014-0109-2 info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs12011-014-0109-2 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Humana Press |
publisher.none.fl_str_mv |
Humana Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269493848440832 |
score |
13.13397 |