Identification of Neospora caninum in aborted bovine fetuses of Southern Chile

Autores
Moroni, Manuel; Navarro, Mauricio; Paredes, Enrrique; Romero, Alex; Alberdi, Aintzane; Lischinsky, Tania; Moore, Dadin Prando; Campero, Carlos Manuel; Uzal, Francisco Alejandro
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to identify Neospora caninum in histologic sections of brain and optic nerve of aborted bovine fetuses. Sections of these tissues from 296 aborted bovine fetuses from Southern Chile, submitted between 2000 and 2010, to the Instituto de Patología Animal of the Universidad Austral de Chile were analyzed. Forty-four (14.9%) fetuses had microscopic lesions compatible with bovine neosporosis, including gliosis, necrosis, intralesional protozoan cysts and neuritis of optic nerves. N. caninum was identified by immunohistochemistry in brain sections of 27 cases (9.1%) with compatible lesions. Positive immunostaining was also found in the optic nerves of 13 cases (4.4%) with neuritis. N. caninum DNA was identified by PCR in brain sections in 31 cases (10.5%). In total, 36 cases (12.2%) were positive by either technique. Phylogenetic analysis of the ITS1 gene sequence of N. caninum revealed a high degree of conservation among different isolates. This is the first report of N. caninum-associated optic neuritis in cattle and also the first report of confirmed bovine abortion associated with this parasite in Chile.
Fil: Moroni, Manuel. Universidad Austral de Chile; Chile
Fil: Navarro, Mauricio. Universidad Austral de Chile; Chile
Fil: Paredes, Enrrique. Universidad Austral de Chile; Chile
Fil: Romero, Alex. Universidad Austral de Chile; Chile
Fil: Alberdi, Aintzane. Universidad Austral de Chile; Chile
Fil: Lischinsky, Tania. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Uzal, Francisco Alejandro. University of California at Davis; Estados Unidos
Materia
NEOSPORA CANINUM
BOVINE ABORTION
IMMUNOHISTOCHEMISTRY
PHYLOGENETIC ANALYSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102026
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Identification of Neospora caninum in aborted bovine fetuses of Southern ChileMoroni, ManuelNavarro, MauricioParedes, EnrriqueRomero, AlexAlberdi, AintzaneLischinsky, TaniaMoore, Dadin PrandoCampero, Carlos ManuelUzal, Francisco AlejandroNEOSPORA CANINUMBOVINE ABORTIONIMMUNOHISTOCHEMISTRYPHYLOGENETIC ANALYSIShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of this study was to identify Neospora caninum in histologic sections of brain and optic nerve of aborted bovine fetuses. Sections of these tissues from 296 aborted bovine fetuses from Southern Chile, submitted between 2000 and 2010, to the Instituto de Patología Animal of the Universidad Austral de Chile were analyzed. Forty-four (14.9%) fetuses had microscopic lesions compatible with bovine neosporosis, including gliosis, necrosis, intralesional protozoan cysts and neuritis of optic nerves. N. caninum was identified by immunohistochemistry in brain sections of 27 cases (9.1%) with compatible lesions. Positive immunostaining was also found in the optic nerves of 13 cases (4.4%) with neuritis. N. caninum DNA was identified by PCR in brain sections in 31 cases (10.5%). In total, 36 cases (12.2%) were positive by either technique. Phylogenetic analysis of the ITS1 gene sequence of N. caninum revealed a high degree of conservation among different isolates. This is the first report of N. caninum-associated optic neuritis in cattle and also the first report of confirmed bovine abortion associated with this parasite in Chile.Fil: Moroni, Manuel. Universidad Austral de Chile; ChileFil: Navarro, Mauricio. Universidad Austral de Chile; ChileFil: Paredes, Enrrique. Universidad Austral de Chile; ChileFil: Romero, Alex. Universidad Austral de Chile; ChileFil: Alberdi, Aintzane. Universidad Austral de Chile; ChileFil: Lischinsky, Tania. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Uzal, Francisco Alejandro. University of California at Davis; Estados UnidosAssociação Brasileira de Patologia Veterinária2018-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102026Moroni, Manuel; Navarro, Mauricio; Paredes, Enrrique; Romero, Alex; Alberdi, Aintzane; et al.; Identification of Neospora caninum in aborted bovine fetuses of Southern Chile; Associação Brasileira de Patologia Veterinária; Brazilian Journal of Veterinary Pathology; 11; 2; 5-2018; 37-411983-0246CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bjvp.org.br/bjvp-archive/2018/07/vol-11-n-2-july-2018/info:eu-repo/semantics/altIdentifier/doi/10.24070/bjvp.1983-0246.v11i2p37-41info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:09:04Zoai:ri.conicet.gov.ar:11336/102026instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:09:04.635CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification of Neospora caninum in aborted bovine fetuses of Southern Chile
title Identification of Neospora caninum in aborted bovine fetuses of Southern Chile
spellingShingle Identification of Neospora caninum in aborted bovine fetuses of Southern Chile
Moroni, Manuel
NEOSPORA CANINUM
BOVINE ABORTION
IMMUNOHISTOCHEMISTRY
PHYLOGENETIC ANALYSIS
title_short Identification of Neospora caninum in aborted bovine fetuses of Southern Chile
title_full Identification of Neospora caninum in aborted bovine fetuses of Southern Chile
title_fullStr Identification of Neospora caninum in aborted bovine fetuses of Southern Chile
title_full_unstemmed Identification of Neospora caninum in aborted bovine fetuses of Southern Chile
title_sort Identification of Neospora caninum in aborted bovine fetuses of Southern Chile
dc.creator.none.fl_str_mv Moroni, Manuel
Navarro, Mauricio
Paredes, Enrrique
Romero, Alex
Alberdi, Aintzane
Lischinsky, Tania
Moore, Dadin Prando
Campero, Carlos Manuel
Uzal, Francisco Alejandro
author Moroni, Manuel
author_facet Moroni, Manuel
Navarro, Mauricio
Paredes, Enrrique
Romero, Alex
Alberdi, Aintzane
Lischinsky, Tania
Moore, Dadin Prando
Campero, Carlos Manuel
Uzal, Francisco Alejandro
author_role author
author2 Navarro, Mauricio
Paredes, Enrrique
Romero, Alex
Alberdi, Aintzane
Lischinsky, Tania
Moore, Dadin Prando
Campero, Carlos Manuel
Uzal, Francisco Alejandro
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv NEOSPORA CANINUM
BOVINE ABORTION
IMMUNOHISTOCHEMISTRY
PHYLOGENETIC ANALYSIS
topic NEOSPORA CANINUM
BOVINE ABORTION
IMMUNOHISTOCHEMISTRY
PHYLOGENETIC ANALYSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of this study was to identify Neospora caninum in histologic sections of brain and optic nerve of aborted bovine fetuses. Sections of these tissues from 296 aborted bovine fetuses from Southern Chile, submitted between 2000 and 2010, to the Instituto de Patología Animal of the Universidad Austral de Chile were analyzed. Forty-four (14.9%) fetuses had microscopic lesions compatible with bovine neosporosis, including gliosis, necrosis, intralesional protozoan cysts and neuritis of optic nerves. N. caninum was identified by immunohistochemistry in brain sections of 27 cases (9.1%) with compatible lesions. Positive immunostaining was also found in the optic nerves of 13 cases (4.4%) with neuritis. N. caninum DNA was identified by PCR in brain sections in 31 cases (10.5%). In total, 36 cases (12.2%) were positive by either technique. Phylogenetic analysis of the ITS1 gene sequence of N. caninum revealed a high degree of conservation among different isolates. This is the first report of N. caninum-associated optic neuritis in cattle and also the first report of confirmed bovine abortion associated with this parasite in Chile.
Fil: Moroni, Manuel. Universidad Austral de Chile; Chile
Fil: Navarro, Mauricio. Universidad Austral de Chile; Chile
Fil: Paredes, Enrrique. Universidad Austral de Chile; Chile
Fil: Romero, Alex. Universidad Austral de Chile; Chile
Fil: Alberdi, Aintzane. Universidad Austral de Chile; Chile
Fil: Lischinsky, Tania. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Uzal, Francisco Alejandro. University of California at Davis; Estados Unidos
description The aim of this study was to identify Neospora caninum in histologic sections of brain and optic nerve of aborted bovine fetuses. Sections of these tissues from 296 aborted bovine fetuses from Southern Chile, submitted between 2000 and 2010, to the Instituto de Patología Animal of the Universidad Austral de Chile were analyzed. Forty-four (14.9%) fetuses had microscopic lesions compatible with bovine neosporosis, including gliosis, necrosis, intralesional protozoan cysts and neuritis of optic nerves. N. caninum was identified by immunohistochemistry in brain sections of 27 cases (9.1%) with compatible lesions. Positive immunostaining was also found in the optic nerves of 13 cases (4.4%) with neuritis. N. caninum DNA was identified by PCR in brain sections in 31 cases (10.5%). In total, 36 cases (12.2%) were positive by either technique. Phylogenetic analysis of the ITS1 gene sequence of N. caninum revealed a high degree of conservation among different isolates. This is the first report of N. caninum-associated optic neuritis in cattle and also the first report of confirmed bovine abortion associated with this parasite in Chile.
publishDate 2018
dc.date.none.fl_str_mv 2018-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102026
Moroni, Manuel; Navarro, Mauricio; Paredes, Enrrique; Romero, Alex; Alberdi, Aintzane; et al.; Identification of Neospora caninum in aborted bovine fetuses of Southern Chile; Associação Brasileira de Patologia Veterinária; Brazilian Journal of Veterinary Pathology; 11; 2; 5-2018; 37-41
1983-0246
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102026
identifier_str_mv Moroni, Manuel; Navarro, Mauricio; Paredes, Enrrique; Romero, Alex; Alberdi, Aintzane; et al.; Identification of Neospora caninum in aborted bovine fetuses of Southern Chile; Associação Brasileira de Patologia Veterinária; Brazilian Journal of Veterinary Pathology; 11; 2; 5-2018; 37-41
1983-0246
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bjvp.org.br/bjvp-archive/2018/07/vol-11-n-2-july-2018/
info:eu-repo/semantics/altIdentifier/doi/10.24070/bjvp.1983-0246.v11i2p37-41
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Patologia Veterinária
publisher.none.fl_str_mv Associação Brasileira de Patologia Veterinária
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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