Cytochrome c deficiency differentially affects the in vivo mitochondrial electron partitioning and primary metabolism depending on the photoperiod

Autores
Florez Sarasa, Igor; Welchen, Elina; Racca, Sofia; Gonzalez, Daniel Hector; Vallarino, José G.; Fernie, Alisdair R.; Ribas Carbo, Miquel; Del Saz, Nestor Fernandez
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plant respiration provides metabolic flexibility under changing environmental conditions by modulating the activity of the nonphosphorylating alternative pathways from the mitochondrial electron transport chain, which bypass the main energy-producing components of the cytochrome oxidase pathway (COP). While adjustments in leaf primary metabolism induced by changes in day length are well studied, possible differences in the in vivo contribution of the COP and the alternative oxidase pathway (AOP) between different photoperiods remain unknown. In our study, in vivo electron partitioning between AOP and COP and expression analysis of respiratory components, photosynthesis, and the levels of primary metabolites were studied in leaves of wild-type (WT) plants and cytochrome c (CYTc) mutants, with reduced levels of COP components, under shortand long-day photoperiods. Our results clearly show that differences in AOP and COP in vivo activities between WT and cytc mutants depend on the photoperiod likely due to energy and stress signaling constraints. Parallel responses observed between in vivo respiratory activities, TCA cycle intermediates, amino acids, and stress signaling metabolites indicate the coordination of different pathways of primary metabolism to support growth adaptation under different photoperiods.
Fil: Florez Sarasa, Igor. Consejo Superior de Investigaciones Científicas; España. Universitat Autònoma de Barcelona; España
Fil: Welchen, Elina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Racca, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Gonzalez, Daniel Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Vallarino, José G.. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Fernie, Alisdair R.. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Ribas Carbo, Miquel. Universitat de Les Illes Balears; España
Fil: Del Saz, Nestor Fernandez. Universidad de Concepción; Chile
Materia
ALTERNATIVE OXIDASE (AOX)
CYTOCHROME C (CYTC)
METABOLITE PROFILING
OXYGEN ISOTOPE DISCRIMINATION
PHOTOPERIOD
PRIMARY METABOLISM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181990
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/181990
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cytochrome c deficiency differentially affects the in vivo mitochondrial electron partitioning and primary metabolism depending on the photoperiodFlorez Sarasa, IgorWelchen, ElinaRacca, SofiaGonzalez, Daniel HectorVallarino, José G.Fernie, Alisdair R.Ribas Carbo, MiquelDel Saz, Nestor FernandezALTERNATIVE OXIDASE (AOX)CYTOCHROME C (CYTC)METABOLITE PROFILINGOXYGEN ISOTOPE DISCRIMINATIONPHOTOPERIODPRIMARY METABOLISMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Plant respiration provides metabolic flexibility under changing environmental conditions by modulating the activity of the nonphosphorylating alternative pathways from the mitochondrial electron transport chain, which bypass the main energy-producing components of the cytochrome oxidase pathway (COP). While adjustments in leaf primary metabolism induced by changes in day length are well studied, possible differences in the in vivo contribution of the COP and the alternative oxidase pathway (AOP) between different photoperiods remain unknown. In our study, in vivo electron partitioning between AOP and COP and expression analysis of respiratory components, photosynthesis, and the levels of primary metabolites were studied in leaves of wild-type (WT) plants and cytochrome c (CYTc) mutants, with reduced levels of COP components, under shortand long-day photoperiods. Our results clearly show that differences in AOP and COP in vivo activities between WT and cytc mutants depend on the photoperiod likely due to energy and stress signaling constraints. Parallel responses observed between in vivo respiratory activities, TCA cycle intermediates, amino acids, and stress signaling metabolites indicate the coordination of different pathways of primary metabolism to support growth adaptation under different photoperiods.Fil: Florez Sarasa, Igor. Consejo Superior de Investigaciones Científicas; España. Universitat Autònoma de Barcelona; EspañaFil: Welchen, Elina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Racca, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Gonzalez, Daniel Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Vallarino, José G.. Max Planck Institute Of Molecular Plant Physiology; AlemaniaFil: Fernie, Alisdair R.. Max Planck Institute Of Molecular Plant Physiology; AlemaniaFil: Ribas Carbo, Miquel. Universitat de Les Illes Balears; EspañaFil: Del Saz, Nestor Fernandez. Universidad de Concepción; ChileMultidisciplinary Digital Publishing Institute2021-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/181990Florez Sarasa, Igor; Welchen, Elina; Racca, Sofia; Gonzalez, Daniel Hector; Vallarino, José G.; et al.; Cytochrome c deficiency differentially affects the in vivo mitochondrial electron partitioning and primary metabolism depending on the photoperiod; Multidisciplinary Digital Publishing Institute; Plants; 10; 3; 3-2021; 1-182223-7747CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/plants10030444info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/10/3/444info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:05:13Zoai:ri.conicet.gov.ar:11336/181990instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:05:13.42CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cytochrome c deficiency differentially affects the in vivo mitochondrial electron partitioning and primary metabolism depending on the photoperiod
title Cytochrome c deficiency differentially affects the in vivo mitochondrial electron partitioning and primary metabolism depending on the photoperiod
spellingShingle Cytochrome c deficiency differentially affects the in vivo mitochondrial electron partitioning and primary metabolism depending on the photoperiod
Florez Sarasa, Igor
ALTERNATIVE OXIDASE (AOX)
CYTOCHROME C (CYTC)
METABOLITE PROFILING
OXYGEN ISOTOPE DISCRIMINATION
PHOTOPERIOD
PRIMARY METABOLISM
title_short Cytochrome c deficiency differentially affects the in vivo mitochondrial electron partitioning and primary metabolism depending on the photoperiod
title_full Cytochrome c deficiency differentially affects the in vivo mitochondrial electron partitioning and primary metabolism depending on the photoperiod
title_fullStr Cytochrome c deficiency differentially affects the in vivo mitochondrial electron partitioning and primary metabolism depending on the photoperiod
title_full_unstemmed Cytochrome c deficiency differentially affects the in vivo mitochondrial electron partitioning and primary metabolism depending on the photoperiod
title_sort Cytochrome c deficiency differentially affects the in vivo mitochondrial electron partitioning and primary metabolism depending on the photoperiod
dc.creator.none.fl_str_mv Florez Sarasa, Igor
Welchen, Elina
Racca, Sofia
Gonzalez, Daniel Hector
Vallarino, José G.
Fernie, Alisdair R.
Ribas Carbo, Miquel
Del Saz, Nestor Fernandez
author Florez Sarasa, Igor
author_facet Florez Sarasa, Igor
Welchen, Elina
Racca, Sofia
Gonzalez, Daniel Hector
Vallarino, José G.
Fernie, Alisdair R.
Ribas Carbo, Miquel
Del Saz, Nestor Fernandez
author_role author
author2 Welchen, Elina
Racca, Sofia
Gonzalez, Daniel Hector
Vallarino, José G.
Fernie, Alisdair R.
Ribas Carbo, Miquel
Del Saz, Nestor Fernandez
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ALTERNATIVE OXIDASE (AOX)
CYTOCHROME C (CYTC)
METABOLITE PROFILING
OXYGEN ISOTOPE DISCRIMINATION
PHOTOPERIOD
PRIMARY METABOLISM
topic ALTERNATIVE OXIDASE (AOX)
CYTOCHROME C (CYTC)
METABOLITE PROFILING
OXYGEN ISOTOPE DISCRIMINATION
PHOTOPERIOD
PRIMARY METABOLISM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Plant respiration provides metabolic flexibility under changing environmental conditions by modulating the activity of the nonphosphorylating alternative pathways from the mitochondrial electron transport chain, which bypass the main energy-producing components of the cytochrome oxidase pathway (COP). While adjustments in leaf primary metabolism induced by changes in day length are well studied, possible differences in the in vivo contribution of the COP and the alternative oxidase pathway (AOP) between different photoperiods remain unknown. In our study, in vivo electron partitioning between AOP and COP and expression analysis of respiratory components, photosynthesis, and the levels of primary metabolites were studied in leaves of wild-type (WT) plants and cytochrome c (CYTc) mutants, with reduced levels of COP components, under shortand long-day photoperiods. Our results clearly show that differences in AOP and COP in vivo activities between WT and cytc mutants depend on the photoperiod likely due to energy and stress signaling constraints. Parallel responses observed between in vivo respiratory activities, TCA cycle intermediates, amino acids, and stress signaling metabolites indicate the coordination of different pathways of primary metabolism to support growth adaptation under different photoperiods.
Fil: Florez Sarasa, Igor. Consejo Superior de Investigaciones Científicas; España. Universitat Autònoma de Barcelona; España
Fil: Welchen, Elina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Racca, Sofia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Gonzalez, Daniel Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Vallarino, José G.. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Fernie, Alisdair R.. Max Planck Institute Of Molecular Plant Physiology; Alemania
Fil: Ribas Carbo, Miquel. Universitat de Les Illes Balears; España
Fil: Del Saz, Nestor Fernandez. Universidad de Concepción; Chile
description Plant respiration provides metabolic flexibility under changing environmental conditions by modulating the activity of the nonphosphorylating alternative pathways from the mitochondrial electron transport chain, which bypass the main energy-producing components of the cytochrome oxidase pathway (COP). While adjustments in leaf primary metabolism induced by changes in day length are well studied, possible differences in the in vivo contribution of the COP and the alternative oxidase pathway (AOP) between different photoperiods remain unknown. In our study, in vivo electron partitioning between AOP and COP and expression analysis of respiratory components, photosynthesis, and the levels of primary metabolites were studied in leaves of wild-type (WT) plants and cytochrome c (CYTc) mutants, with reduced levels of COP components, under shortand long-day photoperiods. Our results clearly show that differences in AOP and COP in vivo activities between WT and cytc mutants depend on the photoperiod likely due to energy and stress signaling constraints. Parallel responses observed between in vivo respiratory activities, TCA cycle intermediates, amino acids, and stress signaling metabolites indicate the coordination of different pathways of primary metabolism to support growth adaptation under different photoperiods.
publishDate 2021
dc.date.none.fl_str_mv 2021-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181990
Florez Sarasa, Igor; Welchen, Elina; Racca, Sofia; Gonzalez, Daniel Hector; Vallarino, José G.; et al.; Cytochrome c deficiency differentially affects the in vivo mitochondrial electron partitioning and primary metabolism depending on the photoperiod; Multidisciplinary Digital Publishing Institute; Plants; 10; 3; 3-2021; 1-18
2223-7747
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181990
identifier_str_mv Florez Sarasa, Igor; Welchen, Elina; Racca, Sofia; Gonzalez, Daniel Hector; Vallarino, José G.; et al.; Cytochrome c deficiency differentially affects the in vivo mitochondrial electron partitioning and primary metabolism depending on the photoperiod; Multidisciplinary Digital Publishing Institute; Plants; 10; 3; 3-2021; 1-18
2223-7747
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/plants10030444
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/10/3/444
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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