Acinetobacter baumannii extensively drug resistant lineages in Buenos Aires hospitals differ from the international clones I–III

Autores
Stietz, Maria Silvina; Ramirez, Maria Soledad; Vilacoba, Elisabet; Merkier, Andrea Karina; Limansky, Adriana Sara; Centrón, Daniela; Catalano, Mariana
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
As a way to contribute to the assessment of Acinetobacter baumannii clinical population structure, multilocus sequence typing (MLST) was performed in a collection of 93 isolates from Buenos Aires (1983? 2012) and Rosario (2006?2009) hospitals. Sequence types (STs) were achieved by Bartual (B) and Institut Pasteur (P) schemes. PFGE typing, antimicrobial susceptibility assays, and the amplification of the OXA carbapenemase genes most prevalent in our region, were also performed. e-Burst clustered the 25 STsB (15 novels) into 5 clonal complexes (CC) and 5 singletons, and grouped the 18 STsP (12 novels) into 3 CC and 4 singletons. Bartual scheme divided the CC79P into two groups. CC113B/CC79P prevailed in Buenos Aires at least in 1992?2009, being responsible for epidemic and for endemic infections and acquiring the XDR (extensively drug-resistant) pattern throughout the years. While, CC119B/CC79P was apparently present before the CC113B/CC79Pdomain. CC103B/CC15P was the second most prevalent CC. Interestingly, CC110B/ST25P apparently increased over the last years. Conversely, CC109B/CC1P (international clone I) predominated in Rosario, although the presence of CC113B/CC79P, CC103B/CC15P and CC110B/ST25P was observed. Nineteen novel STs clustered in CC79P, CC15P, CC113B, CC109B and CC103B, suggesting their clonal expansion during persistence. PFGE typing proved transmission of strains intra- and inter-hospitals in each city. Except for one, all the recent isolates (2007?2012) harboured the blaOXA-23-like. All isolates were susceptible to colistin. Tigecycline MIC90 was 1 mg/L and the rifampicin MIC > 512 mg/l was found among isolates in three hospitals. In conclusion, the international clone II (CC92B/CC2P) was not found among our isolates. CC113B/CC79P, CC103B/CC15P, and ST25P, suggested also as major components in the A. baumannii population together with the international clone I, were present in Buenos Aires and Rosario with different prevalence rate. Their recent isolates showed high distribution of the blaOXA-23-like as well as the XDR pattern.
Fil: Stietz, Maria Silvina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Cátedra de Microbiología, Parasitología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Ramirez, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Vilacoba, Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Merkier, Andrea Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Limansky, Adriana Sara. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Centrón, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Catalano, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Materia
Acinetobacter Baumannii
Clones Internacionales
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1899
Acceder
id CONICETDig_b5c64138126fab416ab570a74389872c
oai_identifier_str oai:ri.conicet.gov.ar:11336/1899
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Acinetobacter baumannii extensively drug resistant lineages in Buenos Aires hospitals differ from the international clones I–IIIStietz, Maria SilvinaRamirez, Maria SoledadVilacoba, ElisabetMerkier, Andrea KarinaLimansky, Adriana SaraCentrón, DanielaCatalano, MarianaAcinetobacter BaumanniiClones Internacionaleshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1As a way to contribute to the assessment of Acinetobacter baumannii clinical population structure, multilocus sequence typing (MLST) was performed in a collection of 93 isolates from Buenos Aires (1983? 2012) and Rosario (2006?2009) hospitals. Sequence types (STs) were achieved by Bartual (B) and Institut Pasteur (P) schemes. PFGE typing, antimicrobial susceptibility assays, and the amplification of the OXA carbapenemase genes most prevalent in our region, were also performed. e-Burst clustered the 25 STsB (15 novels) into 5 clonal complexes (CC) and 5 singletons, and grouped the 18 STsP (12 novels) into 3 CC and 4 singletons. Bartual scheme divided the CC79P into two groups. CC113B/CC79P prevailed in Buenos Aires at least in 1992?2009, being responsible for epidemic and for endemic infections and acquiring the XDR (extensively drug-resistant) pattern throughout the years. While, CC119B/CC79P was apparently present before the CC113B/CC79Pdomain. CC103B/CC15P was the second most prevalent CC. Interestingly, CC110B/ST25P apparently increased over the last years. Conversely, CC109B/CC1P (international clone I) predominated in Rosario, although the presence of CC113B/CC79P, CC103B/CC15P and CC110B/ST25P was observed. Nineteen novel STs clustered in CC79P, CC15P, CC113B, CC109B and CC103B, suggesting their clonal expansion during persistence. PFGE typing proved transmission of strains intra- and inter-hospitals in each city. Except for one, all the recent isolates (2007?2012) harboured the blaOXA-23-like. All isolates were susceptible to colistin. Tigecycline MIC90 was 1 mg/L and the rifampicin MIC > 512 mg/l was found among isolates in three hospitals. In conclusion, the international clone II (CC92B/CC2P) was not found among our isolates. CC113B/CC79P, CC103B/CC15P, and ST25P, suggested also as major components in the A. baumannii population together with the international clone I, were present in Buenos Aires and Rosario with different prevalence rate. Their recent isolates showed high distribution of the blaOXA-23-like as well as the XDR pattern.Fil: Stietz, Maria Silvina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Cátedra de Microbiología, Parasitología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Ramirez, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Vilacoba, Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Merkier, Andrea Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Limansky, Adriana Sara. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Rosario. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Centrón, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Catalano, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaElsevier2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1899Stietz, Maria Silvina; Ramirez, Maria Soledad; Vilacoba, Elisabet; Merkier, Andrea Karina; Limansky, Adriana Sara; et al.; Acinetobacter baumannii extensively drug resistant lineages in Buenos Aires hospitals differ from the international clones I–III; Elsevier; Infection, Genetics and Evolution; 14; 3-2013; 294-3011567-1348enginfo:eu-repo/semantics/reference/url/info:eu-repo/semantics/reference es info:eu-repo/semantics/reference/pmid/23313831info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1567134813000051info:eu-repo/semantics/altIdentifier/doi/doi:10.1016/j.meegid.2012.12.020info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:01:44Zoai:ri.conicet.gov.ar:11336/1899instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:01:44.837CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Acinetobacter baumannii extensively drug resistant lineages in Buenos Aires hospitals differ from the international clones I–III
title Acinetobacter baumannii extensively drug resistant lineages in Buenos Aires hospitals differ from the international clones I–III
spellingShingle Acinetobacter baumannii extensively drug resistant lineages in Buenos Aires hospitals differ from the international clones I–III
Stietz, Maria Silvina
Acinetobacter Baumannii
Clones Internacionales
title_short Acinetobacter baumannii extensively drug resistant lineages in Buenos Aires hospitals differ from the international clones I–III
title_full Acinetobacter baumannii extensively drug resistant lineages in Buenos Aires hospitals differ from the international clones I–III
title_fullStr Acinetobacter baumannii extensively drug resistant lineages in Buenos Aires hospitals differ from the international clones I–III
title_full_unstemmed Acinetobacter baumannii extensively drug resistant lineages in Buenos Aires hospitals differ from the international clones I–III
title_sort Acinetobacter baumannii extensively drug resistant lineages in Buenos Aires hospitals differ from the international clones I–III
dc.creator.none.fl_str_mv Stietz, Maria Silvina
Ramirez, Maria Soledad
Vilacoba, Elisabet
Merkier, Andrea Karina
Limansky, Adriana Sara
Centrón, Daniela
Catalano, Mariana
author Stietz, Maria Silvina
author_facet Stietz, Maria Silvina
Ramirez, Maria Soledad
Vilacoba, Elisabet
Merkier, Andrea Karina
Limansky, Adriana Sara
Centrón, Daniela
Catalano, Mariana
author_role author
author2 Ramirez, Maria Soledad
Vilacoba, Elisabet
Merkier, Andrea Karina
Limansky, Adriana Sara
Centrón, Daniela
Catalano, Mariana
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Acinetobacter Baumannii
Clones Internacionales
topic Acinetobacter Baumannii
Clones Internacionales
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv As a way to contribute to the assessment of Acinetobacter baumannii clinical population structure, multilocus sequence typing (MLST) was performed in a collection of 93 isolates from Buenos Aires (1983? 2012) and Rosario (2006?2009) hospitals. Sequence types (STs) were achieved by Bartual (B) and Institut Pasteur (P) schemes. PFGE typing, antimicrobial susceptibility assays, and the amplification of the OXA carbapenemase genes most prevalent in our region, were also performed. e-Burst clustered the 25 STsB (15 novels) into 5 clonal complexes (CC) and 5 singletons, and grouped the 18 STsP (12 novels) into 3 CC and 4 singletons. Bartual scheme divided the CC79P into two groups. CC113B/CC79P prevailed in Buenos Aires at least in 1992?2009, being responsible for epidemic and for endemic infections and acquiring the XDR (extensively drug-resistant) pattern throughout the years. While, CC119B/CC79P was apparently present before the CC113B/CC79Pdomain. CC103B/CC15P was the second most prevalent CC. Interestingly, CC110B/ST25P apparently increased over the last years. Conversely, CC109B/CC1P (international clone I) predominated in Rosario, although the presence of CC113B/CC79P, CC103B/CC15P and CC110B/ST25P was observed. Nineteen novel STs clustered in CC79P, CC15P, CC113B, CC109B and CC103B, suggesting their clonal expansion during persistence. PFGE typing proved transmission of strains intra- and inter-hospitals in each city. Except for one, all the recent isolates (2007?2012) harboured the blaOXA-23-like. All isolates were susceptible to colistin. Tigecycline MIC90 was 1 mg/L and the rifampicin MIC > 512 mg/l was found among isolates in three hospitals. In conclusion, the international clone II (CC92B/CC2P) was not found among our isolates. CC113B/CC79P, CC103B/CC15P, and ST25P, suggested also as major components in the A. baumannii population together with the international clone I, were present in Buenos Aires and Rosario with different prevalence rate. Their recent isolates showed high distribution of the blaOXA-23-like as well as the XDR pattern.
Fil: Stietz, Maria Silvina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Cátedra de Microbiología, Parasitología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Ramirez, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Vilacoba, Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Merkier, Andrea Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Limansky, Adriana Sara. Universidad Nacional de Rosario; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Centrón, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Catalano, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
description As a way to contribute to the assessment of Acinetobacter baumannii clinical population structure, multilocus sequence typing (MLST) was performed in a collection of 93 isolates from Buenos Aires (1983? 2012) and Rosario (2006?2009) hospitals. Sequence types (STs) were achieved by Bartual (B) and Institut Pasteur (P) schemes. PFGE typing, antimicrobial susceptibility assays, and the amplification of the OXA carbapenemase genes most prevalent in our region, were also performed. e-Burst clustered the 25 STsB (15 novels) into 5 clonal complexes (CC) and 5 singletons, and grouped the 18 STsP (12 novels) into 3 CC and 4 singletons. Bartual scheme divided the CC79P into two groups. CC113B/CC79P prevailed in Buenos Aires at least in 1992?2009, being responsible for epidemic and for endemic infections and acquiring the XDR (extensively drug-resistant) pattern throughout the years. While, CC119B/CC79P was apparently present before the CC113B/CC79Pdomain. CC103B/CC15P was the second most prevalent CC. Interestingly, CC110B/ST25P apparently increased over the last years. Conversely, CC109B/CC1P (international clone I) predominated in Rosario, although the presence of CC113B/CC79P, CC103B/CC15P and CC110B/ST25P was observed. Nineteen novel STs clustered in CC79P, CC15P, CC113B, CC109B and CC103B, suggesting their clonal expansion during persistence. PFGE typing proved transmission of strains intra- and inter-hospitals in each city. Except for one, all the recent isolates (2007?2012) harboured the blaOXA-23-like. All isolates were susceptible to colistin. Tigecycline MIC90 was 1 mg/L and the rifampicin MIC > 512 mg/l was found among isolates in three hospitals. In conclusion, the international clone II (CC92B/CC2P) was not found among our isolates. CC113B/CC79P, CC103B/CC15P, and ST25P, suggested also as major components in the A. baumannii population together with the international clone I, were present in Buenos Aires and Rosario with different prevalence rate. Their recent isolates showed high distribution of the blaOXA-23-like as well as the XDR pattern.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1899
Stietz, Maria Silvina; Ramirez, Maria Soledad; Vilacoba, Elisabet; Merkier, Andrea Karina; Limansky, Adriana Sara; et al.; Acinetobacter baumannii extensively drug resistant lineages in Buenos Aires hospitals differ from the international clones I–III; Elsevier; Infection, Genetics and Evolution; 14; 3-2013; 294-301
1567-1348
url http://hdl.handle.net/11336/1899
identifier_str_mv Stietz, Maria Silvina; Ramirez, Maria Soledad; Vilacoba, Elisabet; Merkier, Andrea Karina; Limansky, Adriana Sara; et al.; Acinetobacter baumannii extensively drug resistant lineages in Buenos Aires hospitals differ from the international clones I–III; Elsevier; Infection, Genetics and Evolution; 14; 3-2013; 294-301
1567-1348
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/reference/url/info:eu-repo/semantics/reference es info:eu-repo/semantics/reference/pmid/23313831
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1567134813000051
info:eu-repo/semantics/altIdentifier/doi/doi:10.1016/j.meegid.2012.12.020
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1847977465990873088
score 13.121305

Publicaciones similares