Biological control of the false root-knot nematode Nacobbus aberrans by Pseudomonas protegens under controlled conditions

Autores
Lax, Paola; Marro, Nicolás Alejandro; Agaras, Betina Cecilia; Valverde, Claudio Fabián; Doucet, Marcelo Edmundo; Becerra, Alejandra Gabriela
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of antagonistic biological agents, such as fungi and bacteria, offers an economical and safe strategy to manage planteparasitic nematodes in infested fields. The false root-knot nematode, Nacobbus aberrans, is a damaging parasite of many agronomic and horticultural crops in South and North America. The management of this nematode is challenging and often not profitable for farmers. In greenhouse tests, conducted in Argentina, applications of the strain Pseudomonas protegens CHA0 and its isogenic derivative ARQ1 (used as control) at a rate of 108 cfu ml1 suppressed infection and reproduction of N. aberrans on tomato roots. However, neither of the strains promoted plant root or shoot growth of the treated plants. Root colonization by the bacteria was assessed by specific PCR-RFLP protocols and fluorescence microscopy. The results obtained in this preliminary study were encouraging and showed the potential of P. protegens CHA0 to be used for the management of the false root-knot nematode on tomato.
Fil: Lax, Paola. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Diversidad y Ecologia Animal; Argentina
Fil: Marro, Nicolás Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Fil: Agaras, Betina Cecilia. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valverde, Claudio Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación En Interacciones Biológicas; Argentina
Fil: Doucet, Marcelo Edmundo. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Diversidad y Ecologia Animal; Argentina
Fil: Becerra, Alejandra Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Materia
Biological Control
Fluorescen Ce Microscopy
Pcr-Rflp
Plant-Parasitic Nematode
Rhizobacterium
Tomato
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8274
Acceder
id CONICETDig_b4b6ae358aa76feb9311889f58688d3c
oai_identifier_str oai:ri.conicet.gov.ar:11336/8274
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Biological control of the false root-knot nematode Nacobbus aberrans by Pseudomonas protegens under controlled conditionsLax, PaolaMarro, Nicolás AlejandroAgaras, Betina CeciliaValverde, Claudio FabiánDoucet, Marcelo EdmundoBecerra, Alejandra GabrielaBiological ControlFluorescen Ce MicroscopyPcr-RflpPlant-Parasitic NematodeRhizobacteriumTomatohttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The use of antagonistic biological agents, such as fungi and bacteria, offers an economical and safe strategy to manage planteparasitic nematodes in infested fields. The false root-knot nematode, Nacobbus aberrans, is a damaging parasite of many agronomic and horticultural crops in South and North America. The management of this nematode is challenging and often not profitable for farmers. In greenhouse tests, conducted in Argentina, applications of the strain Pseudomonas protegens CHA0 and its isogenic derivative ARQ1 (used as control) at a rate of 108 cfu ml1 suppressed infection and reproduction of N. aberrans on tomato roots. However, neither of the strains promoted plant root or shoot growth of the treated plants. Root colonization by the bacteria was assessed by specific PCR-RFLP protocols and fluorescence microscopy. The results obtained in this preliminary study were encouraging and showed the potential of P. protegens CHA0 to be used for the management of the false root-knot nematode on tomato.Fil: Lax, Paola. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Diversidad y Ecologia Animal; ArgentinaFil: Marro, Nicolás Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); ArgentinaFil: Agaras, Betina Cecilia. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Valverde, Claudio Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación En Interacciones Biológicas; ArgentinaFil: Doucet, Marcelo Edmundo. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Diversidad y Ecologia Animal; ArgentinaFil: Becerra, Alejandra Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); ArgentinaElsevier2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8274Lax, Paola; Marro, Nicolás Alejandro; Agaras, Betina Cecilia; Valverde, Claudio Fabián; Doucet, Marcelo Edmundo; et al.; Biological control of the false root-knot nematode Nacobbus aberrans by Pseudomonas protegens under controlled conditions; Elsevier; Crop Protection; 52; 6-2013; 97-1020261-2194enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0261219413001014info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cropro.2013.02.020info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:13Zoai:ri.conicet.gov.ar:11336/8274instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:14.031CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biological control of the false root-knot nematode Nacobbus aberrans by Pseudomonas protegens under controlled conditions
title Biological control of the false root-knot nematode Nacobbus aberrans by Pseudomonas protegens under controlled conditions
spellingShingle Biological control of the false root-knot nematode Nacobbus aberrans by Pseudomonas protegens under controlled conditions
Lax, Paola
Biological Control
Fluorescen Ce Microscopy
Pcr-Rflp
Plant-Parasitic Nematode
Rhizobacterium
Tomato
title_short Biological control of the false root-knot nematode Nacobbus aberrans by Pseudomonas protegens under controlled conditions
title_full Biological control of the false root-knot nematode Nacobbus aberrans by Pseudomonas protegens under controlled conditions
title_fullStr Biological control of the false root-knot nematode Nacobbus aberrans by Pseudomonas protegens under controlled conditions
title_full_unstemmed Biological control of the false root-knot nematode Nacobbus aberrans by Pseudomonas protegens under controlled conditions
title_sort Biological control of the false root-knot nematode Nacobbus aberrans by Pseudomonas protegens under controlled conditions
dc.creator.none.fl_str_mv Lax, Paola
Marro, Nicolás Alejandro
Agaras, Betina Cecilia
Valverde, Claudio Fabián
Doucet, Marcelo Edmundo
Becerra, Alejandra Gabriela
author Lax, Paola
author_facet Lax, Paola
Marro, Nicolás Alejandro
Agaras, Betina Cecilia
Valverde, Claudio Fabián
Doucet, Marcelo Edmundo
Becerra, Alejandra Gabriela
author_role author
author2 Marro, Nicolás Alejandro
Agaras, Betina Cecilia
Valverde, Claudio Fabián
Doucet, Marcelo Edmundo
Becerra, Alejandra Gabriela
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biological Control
Fluorescen Ce Microscopy
Pcr-Rflp
Plant-Parasitic Nematode
Rhizobacterium
Tomato
topic Biological Control
Fluorescen Ce Microscopy
Pcr-Rflp
Plant-Parasitic Nematode
Rhizobacterium
Tomato
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The use of antagonistic biological agents, such as fungi and bacteria, offers an economical and safe strategy to manage planteparasitic nematodes in infested fields. The false root-knot nematode, Nacobbus aberrans, is a damaging parasite of many agronomic and horticultural crops in South and North America. The management of this nematode is challenging and often not profitable for farmers. In greenhouse tests, conducted in Argentina, applications of the strain Pseudomonas protegens CHA0 and its isogenic derivative ARQ1 (used as control) at a rate of 108 cfu ml1 suppressed infection and reproduction of N. aberrans on tomato roots. However, neither of the strains promoted plant root or shoot growth of the treated plants. Root colonization by the bacteria was assessed by specific PCR-RFLP protocols and fluorescence microscopy. The results obtained in this preliminary study were encouraging and showed the potential of P. protegens CHA0 to be used for the management of the false root-knot nematode on tomato.
Fil: Lax, Paola. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Diversidad y Ecologia Animal; Argentina
Fil: Marro, Nicolás Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Fil: Agaras, Betina Cecilia. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valverde, Claudio Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación En Interacciones Biológicas; Argentina
Fil: Doucet, Marcelo Edmundo. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Diversidad y Ecologia Animal; Argentina
Fil: Becerra, Alejandra Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
description The use of antagonistic biological agents, such as fungi and bacteria, offers an economical and safe strategy to manage planteparasitic nematodes in infested fields. The false root-knot nematode, Nacobbus aberrans, is a damaging parasite of many agronomic and horticultural crops in South and North America. The management of this nematode is challenging and often not profitable for farmers. In greenhouse tests, conducted in Argentina, applications of the strain Pseudomonas protegens CHA0 and its isogenic derivative ARQ1 (used as control) at a rate of 108 cfu ml1 suppressed infection and reproduction of N. aberrans on tomato roots. However, neither of the strains promoted plant root or shoot growth of the treated plants. Root colonization by the bacteria was assessed by specific PCR-RFLP protocols and fluorescence microscopy. The results obtained in this preliminary study were encouraging and showed the potential of P. protegens CHA0 to be used for the management of the false root-knot nematode on tomato.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8274
Lax, Paola; Marro, Nicolás Alejandro; Agaras, Betina Cecilia; Valverde, Claudio Fabián; Doucet, Marcelo Edmundo; et al.; Biological control of the false root-knot nematode Nacobbus aberrans by Pseudomonas protegens under controlled conditions; Elsevier; Crop Protection; 52; 6-2013; 97-102
0261-2194
url http://hdl.handle.net/11336/8274
identifier_str_mv Lax, Paola; Marro, Nicolás Alejandro; Agaras, Betina Cecilia; Valverde, Claudio Fabián; Doucet, Marcelo Edmundo; et al.; Biological control of the false root-knot nematode Nacobbus aberrans by Pseudomonas protegens under controlled conditions; Elsevier; Crop Protection; 52; 6-2013; 97-102
0261-2194
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0261219413001014
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cropro.2013.02.020
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269900505088000
score 13.13397

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