Epichloë Fungal Endophytes Influence Seed-Associated Bacterial Communities

Autores
Bastías, Daniel A.; Bubica Bustos, Ludmila Micaela; Jáuregui, Ruy; Barrera, Andrea; Acuña Rodríguez, Ian S.; Molina Montenegro, Marco A.; Gundel, Pedro Emilio
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Seeds commonly harbour diverse bacterial communities that can enhance the fitness of future plants. The bacterial microbiota associated with mother plant’s foliar tissues is one of the main sources of bacteria for seeds. Therefore, any ecological factor influencing the mother plant’s microbiota may also affect the diversity of the seed’s bacterial community. Grasses form associations with beneficial vertically transmitted fungal endophytes of genus Epichloë. The interaction of plants with Epichloë endophytes and insect herbivores can influence the plant foliar microbiota. However, it is unknown whether these interactions (alone or in concert) can affect the assembly of bacterial communities in the produced seed. We subjected Lolium multiflorum plants with and without its common endophyte Epichloë occultans (E+, E-, respectively) to an herbivory treatment with Rhopalosiphum padi aphids and assessed the diversity and composition of the bacterial communities in the produced seed. The presence of Epichloë endophytes influenced the seed bacterial microbiota by increasing the diversity and affecting the composition of the communities. The relative abundances of the bacterial taxa were more similarly distributed in communities associated with E+ than E- seeds with the latter being dominated by just a few bacterial groups. Contrary to our expectations, seed bacterial communities were not affected by the aphid herbivory experienced by mother plants. We speculate that the enhanced seed/seedling performance documented for Epichloë-host associations may be explained, at least in part, by the Epichloë-mediated increment in the seed-bacterial diversity, and that this phenomenon may be applicable to other plant-endophyte associations.
Fil: Bastías, Daniel A.. Grasslands Research Centre; Nueva Zelanda
Fil: Bubica Bustos, Ludmila Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Jáuregui, Ruy. Grasslands Research Centre; Nueva Zelanda
Fil: Barrera, Andrea. Universidad de Talca; Chile
Fil: Acuña Rodríguez, Ian S.. Universidad de Talca; Chile
Fil: Molina Montenegro, Marco A.. Universidad de Talca; Chile. Universidad Católica del Norte; Chile. Universidad Católica del Maule; Chile
Fil: Gundel, Pedro Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Universidad de Talca; Chile
Materia
EPICHLOË ENDOPHYTES
HERBIVORY
PLANT-ASSOCIATED BACTERIAL COMMUNITIES
PLANT-MICROBE INTERACTIONS
SEED MICROBIOTA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/216271
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/216271
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Epichloë Fungal Endophytes Influence Seed-Associated Bacterial CommunitiesBastías, Daniel A.Bubica Bustos, Ludmila MicaelaJáuregui, RuyBarrera, AndreaAcuña Rodríguez, Ian S.Molina Montenegro, Marco A.Gundel, Pedro EmilioEPICHLOË ENDOPHYTESHERBIVORYPLANT-ASSOCIATED BACTERIAL COMMUNITIESPLANT-MICROBE INTERACTIONSSEED MICROBIOTAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Seeds commonly harbour diverse bacterial communities that can enhance the fitness of future plants. The bacterial microbiota associated with mother plant’s foliar tissues is one of the main sources of bacteria for seeds. Therefore, any ecological factor influencing the mother plant’s microbiota may also affect the diversity of the seed’s bacterial community. Grasses form associations with beneficial vertically transmitted fungal endophytes of genus Epichloë. The interaction of plants with Epichloë endophytes and insect herbivores can influence the plant foliar microbiota. However, it is unknown whether these interactions (alone or in concert) can affect the assembly of bacterial communities in the produced seed. We subjected Lolium multiflorum plants with and without its common endophyte Epichloë occultans (E+, E-, respectively) to an herbivory treatment with Rhopalosiphum padi aphids and assessed the diversity and composition of the bacterial communities in the produced seed. The presence of Epichloë endophytes influenced the seed bacterial microbiota by increasing the diversity and affecting the composition of the communities. The relative abundances of the bacterial taxa were more similarly distributed in communities associated with E+ than E- seeds with the latter being dominated by just a few bacterial groups. Contrary to our expectations, seed bacterial communities were not affected by the aphid herbivory experienced by mother plants. We speculate that the enhanced seed/seedling performance documented for Epichloë-host associations may be explained, at least in part, by the Epichloë-mediated increment in the seed-bacterial diversity, and that this phenomenon may be applicable to other plant-endophyte associations.Fil: Bastías, Daniel A.. Grasslands Research Centre; Nueva ZelandaFil: Bubica Bustos, Ludmila Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Jáuregui, Ruy. Grasslands Research Centre; Nueva ZelandaFil: Barrera, Andrea. Universidad de Talca; ChileFil: Acuña Rodríguez, Ian S.. Universidad de Talca; ChileFil: Molina Montenegro, Marco A.. Universidad de Talca; Chile. Universidad Católica del Norte; Chile. Universidad Católica del Maule; ChileFil: Gundel, Pedro Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Universidad de Talca; ChileFrontiers Media2022-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/216271Bastías, Daniel A.; Bubica Bustos, Ludmila Micaela; Jáuregui, Ruy; Barrera, Andrea; Acuña Rodríguez, Ian S.; et al.; Epichloë Fungal Endophytes Influence Seed-Associated Bacterial Communities; Frontiers Media; Frontiers in Microbiology; 12; 1-2022; 1-111664-302XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2021.795354/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2021.795354info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:59:32Zoai:ri.conicet.gov.ar:11336/216271instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:59:32.664CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Epichloë Fungal Endophytes Influence Seed-Associated Bacterial Communities
title Epichloë Fungal Endophytes Influence Seed-Associated Bacterial Communities
spellingShingle Epichloë Fungal Endophytes Influence Seed-Associated Bacterial Communities
Bastías, Daniel A.
EPICHLOË ENDOPHYTES
HERBIVORY
PLANT-ASSOCIATED BACTERIAL COMMUNITIES
PLANT-MICROBE INTERACTIONS
SEED MICROBIOTA
title_short Epichloë Fungal Endophytes Influence Seed-Associated Bacterial Communities
title_full Epichloë Fungal Endophytes Influence Seed-Associated Bacterial Communities
title_fullStr Epichloë Fungal Endophytes Influence Seed-Associated Bacterial Communities
title_full_unstemmed Epichloë Fungal Endophytes Influence Seed-Associated Bacterial Communities
title_sort Epichloë Fungal Endophytes Influence Seed-Associated Bacterial Communities
dc.creator.none.fl_str_mv Bastías, Daniel A.
Bubica Bustos, Ludmila Micaela
Jáuregui, Ruy
Barrera, Andrea
Acuña Rodríguez, Ian S.
Molina Montenegro, Marco A.
Gundel, Pedro Emilio
author Bastías, Daniel A.
author_facet Bastías, Daniel A.
Bubica Bustos, Ludmila Micaela
Jáuregui, Ruy
Barrera, Andrea
Acuña Rodríguez, Ian S.
Molina Montenegro, Marco A.
Gundel, Pedro Emilio
author_role author
author2 Bubica Bustos, Ludmila Micaela
Jáuregui, Ruy
Barrera, Andrea
Acuña Rodríguez, Ian S.
Molina Montenegro, Marco A.
Gundel, Pedro Emilio
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv EPICHLOË ENDOPHYTES
HERBIVORY
PLANT-ASSOCIATED BACTERIAL COMMUNITIES
PLANT-MICROBE INTERACTIONS
SEED MICROBIOTA
topic EPICHLOË ENDOPHYTES
HERBIVORY
PLANT-ASSOCIATED BACTERIAL COMMUNITIES
PLANT-MICROBE INTERACTIONS
SEED MICROBIOTA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Seeds commonly harbour diverse bacterial communities that can enhance the fitness of future plants. The bacterial microbiota associated with mother plant’s foliar tissues is one of the main sources of bacteria for seeds. Therefore, any ecological factor influencing the mother plant’s microbiota may also affect the diversity of the seed’s bacterial community. Grasses form associations with beneficial vertically transmitted fungal endophytes of genus Epichloë. The interaction of plants with Epichloë endophytes and insect herbivores can influence the plant foliar microbiota. However, it is unknown whether these interactions (alone or in concert) can affect the assembly of bacterial communities in the produced seed. We subjected Lolium multiflorum plants with and without its common endophyte Epichloë occultans (E+, E-, respectively) to an herbivory treatment with Rhopalosiphum padi aphids and assessed the diversity and composition of the bacterial communities in the produced seed. The presence of Epichloë endophytes influenced the seed bacterial microbiota by increasing the diversity and affecting the composition of the communities. The relative abundances of the bacterial taxa were more similarly distributed in communities associated with E+ than E- seeds with the latter being dominated by just a few bacterial groups. Contrary to our expectations, seed bacterial communities were not affected by the aphid herbivory experienced by mother plants. We speculate that the enhanced seed/seedling performance documented for Epichloë-host associations may be explained, at least in part, by the Epichloë-mediated increment in the seed-bacterial diversity, and that this phenomenon may be applicable to other plant-endophyte associations.
Fil: Bastías, Daniel A.. Grasslands Research Centre; Nueva Zelanda
Fil: Bubica Bustos, Ludmila Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Jáuregui, Ruy. Grasslands Research Centre; Nueva Zelanda
Fil: Barrera, Andrea. Universidad de Talca; Chile
Fil: Acuña Rodríguez, Ian S.. Universidad de Talca; Chile
Fil: Molina Montenegro, Marco A.. Universidad de Talca; Chile. Universidad Católica del Norte; Chile. Universidad Católica del Maule; Chile
Fil: Gundel, Pedro Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Universidad de Talca; Chile
description Seeds commonly harbour diverse bacterial communities that can enhance the fitness of future plants. The bacterial microbiota associated with mother plant’s foliar tissues is one of the main sources of bacteria for seeds. Therefore, any ecological factor influencing the mother plant’s microbiota may also affect the diversity of the seed’s bacterial community. Grasses form associations with beneficial vertically transmitted fungal endophytes of genus Epichloë. The interaction of plants with Epichloë endophytes and insect herbivores can influence the plant foliar microbiota. However, it is unknown whether these interactions (alone or in concert) can affect the assembly of bacterial communities in the produced seed. We subjected Lolium multiflorum plants with and without its common endophyte Epichloë occultans (E+, E-, respectively) to an herbivory treatment with Rhopalosiphum padi aphids and assessed the diversity and composition of the bacterial communities in the produced seed. The presence of Epichloë endophytes influenced the seed bacterial microbiota by increasing the diversity and affecting the composition of the communities. The relative abundances of the bacterial taxa were more similarly distributed in communities associated with E+ than E- seeds with the latter being dominated by just a few bacterial groups. Contrary to our expectations, seed bacterial communities were not affected by the aphid herbivory experienced by mother plants. We speculate that the enhanced seed/seedling performance documented for Epichloë-host associations may be explained, at least in part, by the Epichloë-mediated increment in the seed-bacterial diversity, and that this phenomenon may be applicable to other plant-endophyte associations.
publishDate 2022
dc.date.none.fl_str_mv 2022-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/216271
Bastías, Daniel A.; Bubica Bustos, Ludmila Micaela; Jáuregui, Ruy; Barrera, Andrea; Acuña Rodríguez, Ian S.; et al.; Epichloë Fungal Endophytes Influence Seed-Associated Bacterial Communities; Frontiers Media; Frontiers in Microbiology; 12; 1-2022; 1-11
1664-302X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/216271
identifier_str_mv Bastías, Daniel A.; Bubica Bustos, Ludmila Micaela; Jáuregui, Ruy; Barrera, Andrea; Acuña Rodríguez, Ian S.; et al.; Epichloë Fungal Endophytes Influence Seed-Associated Bacterial Communities; Frontiers Media; Frontiers in Microbiology; 12; 1-2022; 1-11
1664-302X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fmicb.2021.795354/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fmicb.2021.795354
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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