Nitric oxide is required for determining root architecture and lignin composition in sunflower. Supporting evidence from microarray analyses

Autores
Corti Monzón, Georgina de la Paz; Pinedo, Marcela Lilian; Di Rienzo, Julio Alejandro; Novo Uzal, Esther; Pomar, Federico; Lamattina, Lorenzo; de la Canal, Laura
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nitric oxide (NO) is a signal molecule involved in several physiological processes in plants, including root development. Despite the importance of NO as a root growth regulator, the knowledge about the genes and metabolic pathways modulated by NO in this process is still limited. A constraint to unravel these pathways has been the use of exogenous applications of NO donors that may produce toxic effects. We have analyzed the role of NO in root architecture through the depletion of endogenous NO using the scavenger cPTIO. Sunflower seedlings growing in liquid medium supplemented with cPTIO showed unaltered primary root length while the number of lateral roots was deeply reduced; indicating that endogenous NO participates in determining root branching in sunflower. The transcriptional changes induced by NO depletion have been analyzed using a large-scale approach. A microarray analysis showed 330 genes regulated in the roots (p ⩽ 0.001) upon endogenous NO depletion. A general cPTIO-induced up-regulation of genes involved in the lignin biosynthetic pathway was observed. Even if no detectable changes in total lignin content could be detected, cell walls analyses revealed that the ratio G/S lignin increased in roots treated with cPTIO. This means that endogenous NO may control lignin composition in planta. Our results suggest that a fine tuning regulation of NO levels could be used by plants to regulate root architecture and lignin composition. The functional implications of these findings are discussed.
Fil: Corti Monzón, Georgina de la Paz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Pinedo, Marcela Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Di Rienzo, Julio Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Matemáticas. Cátedra de Estadística y Biometria; Argentina
Fil: Novo Uzal, Esther. Universidad de Murcia; España
Fil: Pomar, Federico. Universidad da Coruña; España
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
Cptio
Lignin
Microarray
Nitric Oxide
Phenylpropanoid Biosynthesis
Root Architecture
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/34395
Acceder
id CONICETDig_b42dc3382fc5e11e15f68739863834de
oai_identifier_str oai:ri.conicet.gov.ar:11336/34395
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nitric oxide is required for determining root architecture and lignin composition in sunflower. Supporting evidence from microarray analysesCorti Monzón, Georgina de la PazPinedo, Marcela LilianDi Rienzo, Julio AlejandroNovo Uzal, EstherPomar, FedericoLamattina, Lorenzode la Canal, LauraCptioLigninMicroarrayNitric OxidePhenylpropanoid BiosynthesisRoot Architecturehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Nitric oxide (NO) is a signal molecule involved in several physiological processes in plants, including root development. Despite the importance of NO as a root growth regulator, the knowledge about the genes and metabolic pathways modulated by NO in this process is still limited. A constraint to unravel these pathways has been the use of exogenous applications of NO donors that may produce toxic effects. We have analyzed the role of NO in root architecture through the depletion of endogenous NO using the scavenger cPTIO. Sunflower seedlings growing in liquid medium supplemented with cPTIO showed unaltered primary root length while the number of lateral roots was deeply reduced; indicating that endogenous NO participates in determining root branching in sunflower. The transcriptional changes induced by NO depletion have been analyzed using a large-scale approach. A microarray analysis showed 330 genes regulated in the roots (p ⩽ 0.001) upon endogenous NO depletion. A general cPTIO-induced up-regulation of genes involved in the lignin biosynthetic pathway was observed. Even if no detectable changes in total lignin content could be detected, cell walls analyses revealed that the ratio G/S lignin increased in roots treated with cPTIO. This means that endogenous NO may control lignin composition in planta. Our results suggest that a fine tuning regulation of NO levels could be used by plants to regulate root architecture and lignin composition. The functional implications of these findings are discussed.Fil: Corti Monzón, Georgina de la Paz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Pinedo, Marcela Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: Di Rienzo, Julio Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Matemáticas. Cátedra de Estadística y Biometria; ArgentinaFil: Novo Uzal, Esther. Universidad de Murcia; EspañaFil: Pomar, Federico. Universidad da Coruña; EspañaFil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaAcademic Press Inc Elsevier Science2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/34395Corti Monzón, Georgina de la Paz; Pinedo, Marcela Lilian; Di Rienzo, Julio Alejandro; Novo Uzal, Esther ; Pomar, Federico; et al.; Nitric oxide is required for determining root architecture and lignin composition in sunflower. Supporting evidence from microarray analyses; Academic Press Inc Elsevier Science; Nitric Oxide-biology and Chemistry; 39; 4-2014; 20-281089-8603CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.niox.2014.04.004info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S108986031400202Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:42:56Zoai:ri.conicet.gov.ar:11336/34395instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:42:56.34CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nitric oxide is required for determining root architecture and lignin composition in sunflower. Supporting evidence from microarray analyses
title Nitric oxide is required for determining root architecture and lignin composition in sunflower. Supporting evidence from microarray analyses
spellingShingle Nitric oxide is required for determining root architecture and lignin composition in sunflower. Supporting evidence from microarray analyses
Corti Monzón, Georgina de la Paz
Cptio
Lignin
Microarray
Nitric Oxide
Phenylpropanoid Biosynthesis
Root Architecture
title_short Nitric oxide is required for determining root architecture and lignin composition in sunflower. Supporting evidence from microarray analyses
title_full Nitric oxide is required for determining root architecture and lignin composition in sunflower. Supporting evidence from microarray analyses
title_fullStr Nitric oxide is required for determining root architecture and lignin composition in sunflower. Supporting evidence from microarray analyses
title_full_unstemmed Nitric oxide is required for determining root architecture and lignin composition in sunflower. Supporting evidence from microarray analyses
title_sort Nitric oxide is required for determining root architecture and lignin composition in sunflower. Supporting evidence from microarray analyses
dc.creator.none.fl_str_mv Corti Monzón, Georgina de la Paz
Pinedo, Marcela Lilian
Di Rienzo, Julio Alejandro
Novo Uzal, Esther
Pomar, Federico
Lamattina, Lorenzo
de la Canal, Laura
author Corti Monzón, Georgina de la Paz
author_facet Corti Monzón, Georgina de la Paz
Pinedo, Marcela Lilian
Di Rienzo, Julio Alejandro
Novo Uzal, Esther
Pomar, Federico
Lamattina, Lorenzo
de la Canal, Laura
author_role author
author2 Pinedo, Marcela Lilian
Di Rienzo, Julio Alejandro
Novo Uzal, Esther
Pomar, Federico
Lamattina, Lorenzo
de la Canal, Laura
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cptio
Lignin
Microarray
Nitric Oxide
Phenylpropanoid Biosynthesis
Root Architecture
topic Cptio
Lignin
Microarray
Nitric Oxide
Phenylpropanoid Biosynthesis
Root Architecture
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Nitric oxide (NO) is a signal molecule involved in several physiological processes in plants, including root development. Despite the importance of NO as a root growth regulator, the knowledge about the genes and metabolic pathways modulated by NO in this process is still limited. A constraint to unravel these pathways has been the use of exogenous applications of NO donors that may produce toxic effects. We have analyzed the role of NO in root architecture through the depletion of endogenous NO using the scavenger cPTIO. Sunflower seedlings growing in liquid medium supplemented with cPTIO showed unaltered primary root length while the number of lateral roots was deeply reduced; indicating that endogenous NO participates in determining root branching in sunflower. The transcriptional changes induced by NO depletion have been analyzed using a large-scale approach. A microarray analysis showed 330 genes regulated in the roots (p ⩽ 0.001) upon endogenous NO depletion. A general cPTIO-induced up-regulation of genes involved in the lignin biosynthetic pathway was observed. Even if no detectable changes in total lignin content could be detected, cell walls analyses revealed that the ratio G/S lignin increased in roots treated with cPTIO. This means that endogenous NO may control lignin composition in planta. Our results suggest that a fine tuning regulation of NO levels could be used by plants to regulate root architecture and lignin composition. The functional implications of these findings are discussed.
Fil: Corti Monzón, Georgina de la Paz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Pinedo, Marcela Lilian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: Di Rienzo, Julio Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Matemáticas. Cátedra de Estadística y Biometria; Argentina
Fil: Novo Uzal, Esther. Universidad de Murcia; España
Fil: Pomar, Federico. Universidad da Coruña; España
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Fil: de la Canal, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description Nitric oxide (NO) is a signal molecule involved in several physiological processes in plants, including root development. Despite the importance of NO as a root growth regulator, the knowledge about the genes and metabolic pathways modulated by NO in this process is still limited. A constraint to unravel these pathways has been the use of exogenous applications of NO donors that may produce toxic effects. We have analyzed the role of NO in root architecture through the depletion of endogenous NO using the scavenger cPTIO. Sunflower seedlings growing in liquid medium supplemented with cPTIO showed unaltered primary root length while the number of lateral roots was deeply reduced; indicating that endogenous NO participates in determining root branching in sunflower. The transcriptional changes induced by NO depletion have been analyzed using a large-scale approach. A microarray analysis showed 330 genes regulated in the roots (p ⩽ 0.001) upon endogenous NO depletion. A general cPTIO-induced up-regulation of genes involved in the lignin biosynthetic pathway was observed. Even if no detectable changes in total lignin content could be detected, cell walls analyses revealed that the ratio G/S lignin increased in roots treated with cPTIO. This means that endogenous NO may control lignin composition in planta. Our results suggest that a fine tuning regulation of NO levels could be used by plants to regulate root architecture and lignin composition. The functional implications of these findings are discussed.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/34395
Corti Monzón, Georgina de la Paz; Pinedo, Marcela Lilian; Di Rienzo, Julio Alejandro; Novo Uzal, Esther ; Pomar, Federico; et al.; Nitric oxide is required for determining root architecture and lignin composition in sunflower. Supporting evidence from microarray analyses; Academic Press Inc Elsevier Science; Nitric Oxide-biology and Chemistry; 39; 4-2014; 20-28
1089-8603
CONICET Digital
CONICET
url http://hdl.handle.net/11336/34395
identifier_str_mv Corti Monzón, Georgina de la Paz; Pinedo, Marcela Lilian; Di Rienzo, Julio Alejandro; Novo Uzal, Esther ; Pomar, Federico; et al.; Nitric oxide is required for determining root architecture and lignin composition in sunflower. Supporting evidence from microarray analyses; Academic Press Inc Elsevier Science; Nitric Oxide-biology and Chemistry; 39; 4-2014; 20-28
1089-8603
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.niox.2014.04.004
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S108986031400202X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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