Thymopentin improves the survival of septic mice by promoting the production of 15-deoxy-prostaglandin J2 and activating the PPARγ signaling pathway
- Autores
- Zhang, Ye; Yang, Xue; Yan, Wenchao; Li, Rui; Ye, Qian; You, Linjun; Xie, Wenhao; Mo, Kun; Fu, Ruifeng; Wang, Yanxiang; Chen, Yufei; Hou, Hui; Yang, Yong; Birnbaumer, Lutz; Di, Qin; Li, Xianjing
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Sepsis, a systemic inflammatory response syndrome (SIRS) caused by infection, is a major public health concern with limited therapeutic options. Infection disturbs the homeostasis of host, resulting in excessive inflammation and immune suppression. This has prompted the clinical use of immunomodulators to balance host response as an alternative therapeutic strategy. Here, we report that Thymopentin (TP5), a synthetic immunomodulator pentapeptide (Arg-Lys-Asp-Val-Tyr) with an excellent safety profile in the clinic, protects mice against cecal ligation and puncture (CLP)-induced sepsis, as shown by improved survival rate, decreased level of pro-inflammatory cytokines and reduced ratios of macrophages and neutrophils in spleen and peritoneum. Regarding mechanism, TP5 changed the characteristics of LPS-stimulated macrophages by increasing the production of 15-deoxy-Δ12,14-prostaglandin J2 (15-d-PGJ2). In addition, the improved effect of TP5 on survival rates was abolished by the peroxisome proliferator-activated receptor γ (PPARγ) antagonist GW9662. Our results uncover the mechanism of the TP5 protective effects on CLP-induced sepsis and shed light on the development of TP5 as a therapeutic strategy for lethal systemic inflammatory disorders.
Fil: Zhang, Ye. China Pharmaceutical University; China
Fil: Yang, Xue. China Pharmaceutical University; China
Fil: Yan, Wenchao. China Pharmaceutical University; China
Fil: Li, Rui. China Pharmaceutical University; China
Fil: Ye, Qian. China Pharmaceutical University; China
Fil: You, Linjun. China Pharmaceutical University; China
Fil: Xie, Wenhao. China Pharmaceutical University; China
Fil: Mo, Kun. China Pharmaceutical University; China
Fil: Fu, Ruifeng. China Pharmaceutical University; China
Fil: Wang, Yanxiang. China Pharmaceutical University; China
Fil: Chen, Yufei. China Pharmaceutical University; China
Fil: Hou, Hui. China Pharmaceutical University; China
Fil: Yang, Yong. China Pharmaceutical University; China
Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Di, Qin. Nanjing Sport Institute; China
Fil: Li, Xianjing. China Pharmaceutical University; China - Materia
-
15-D-PGJ2
MACROPHAGE
PPARΓ
SEPSIS
THYMOPENTIN - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/140666
Ver los metadatos del registro completo
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spelling |
Thymopentin improves the survival of septic mice by promoting the production of 15-deoxy-prostaglandin J2 and activating the PPARγ signaling pathwayZhang, YeYang, XueYan, WenchaoLi, RuiYe, QianYou, LinjunXie, WenhaoMo, KunFu, RuifengWang, YanxiangChen, YufeiHou, HuiYang, YongBirnbaumer, LutzDi, QinLi, Xianjing15-D-PGJ2MACROPHAGEPPARΓSEPSISTHYMOPENTINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sepsis, a systemic inflammatory response syndrome (SIRS) caused by infection, is a major public health concern with limited therapeutic options. Infection disturbs the homeostasis of host, resulting in excessive inflammation and immune suppression. This has prompted the clinical use of immunomodulators to balance host response as an alternative therapeutic strategy. Here, we report that Thymopentin (TP5), a synthetic immunomodulator pentapeptide (Arg-Lys-Asp-Val-Tyr) with an excellent safety profile in the clinic, protects mice against cecal ligation and puncture (CLP)-induced sepsis, as shown by improved survival rate, decreased level of pro-inflammatory cytokines and reduced ratios of macrophages and neutrophils in spleen and peritoneum. Regarding mechanism, TP5 changed the characteristics of LPS-stimulated macrophages by increasing the production of 15-deoxy-Δ12,14-prostaglandin J2 (15-d-PGJ2). In addition, the improved effect of TP5 on survival rates was abolished by the peroxisome proliferator-activated receptor γ (PPARγ) antagonist GW9662. Our results uncover the mechanism of the TP5 protective effects on CLP-induced sepsis and shed light on the development of TP5 as a therapeutic strategy for lethal systemic inflammatory disorders.Fil: Zhang, Ye. China Pharmaceutical University; ChinaFil: Yang, Xue. China Pharmaceutical University; ChinaFil: Yan, Wenchao. China Pharmaceutical University; ChinaFil: Li, Rui. China Pharmaceutical University; ChinaFil: Ye, Qian. China Pharmaceutical University; ChinaFil: You, Linjun. China Pharmaceutical University; ChinaFil: Xie, Wenhao. China Pharmaceutical University; ChinaFil: Mo, Kun. China Pharmaceutical University; ChinaFil: Fu, Ruifeng. China Pharmaceutical University; ChinaFil: Wang, Yanxiang. China Pharmaceutical University; ChinaFil: Chen, Yufei. China Pharmaceutical University; ChinaFil: Hou, Hui. China Pharmaceutical University; ChinaFil: Yang, Yong. China Pharmaceutical University; ChinaFil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaFil: Di, Qin. Nanjing Sport Institute; ChinaFil: Li, Xianjing. China Pharmaceutical University; ChinaFederation of American Societies for Experimental Biology2020-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/140666Zhang, Ye; Yang, Xue; Yan, Wenchao; Li, Rui; Ye, Qian; et al.; Thymopentin improves the survival of septic mice by promoting the production of 15-deoxy-prostaglandin J2 and activating the PPARγ signaling pathway; Federation of American Societies for Experimental Biology; FASEB Journal; 34; 9; 9-2020; 11772-117850892-6638CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1096/fj.202000467Rinfo:eu-repo/semantics/altIdentifier/url/https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202000467Rinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:25Zoai:ri.conicet.gov.ar:11336/140666instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:26.193CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Thymopentin improves the survival of septic mice by promoting the production of 15-deoxy-prostaglandin J2 and activating the PPARγ signaling pathway |
title |
Thymopentin improves the survival of septic mice by promoting the production of 15-deoxy-prostaglandin J2 and activating the PPARγ signaling pathway |
spellingShingle |
Thymopentin improves the survival of septic mice by promoting the production of 15-deoxy-prostaglandin J2 and activating the PPARγ signaling pathway Zhang, Ye 15-D-PGJ2 MACROPHAGE PPARΓ SEPSIS THYMOPENTIN |
title_short |
Thymopentin improves the survival of septic mice by promoting the production of 15-deoxy-prostaglandin J2 and activating the PPARγ signaling pathway |
title_full |
Thymopentin improves the survival of septic mice by promoting the production of 15-deoxy-prostaglandin J2 and activating the PPARγ signaling pathway |
title_fullStr |
Thymopentin improves the survival of septic mice by promoting the production of 15-deoxy-prostaglandin J2 and activating the PPARγ signaling pathway |
title_full_unstemmed |
Thymopentin improves the survival of septic mice by promoting the production of 15-deoxy-prostaglandin J2 and activating the PPARγ signaling pathway |
title_sort |
Thymopentin improves the survival of septic mice by promoting the production of 15-deoxy-prostaglandin J2 and activating the PPARγ signaling pathway |
dc.creator.none.fl_str_mv |
Zhang, Ye Yang, Xue Yan, Wenchao Li, Rui Ye, Qian You, Linjun Xie, Wenhao Mo, Kun Fu, Ruifeng Wang, Yanxiang Chen, Yufei Hou, Hui Yang, Yong Birnbaumer, Lutz Di, Qin Li, Xianjing |
author |
Zhang, Ye |
author_facet |
Zhang, Ye Yang, Xue Yan, Wenchao Li, Rui Ye, Qian You, Linjun Xie, Wenhao Mo, Kun Fu, Ruifeng Wang, Yanxiang Chen, Yufei Hou, Hui Yang, Yong Birnbaumer, Lutz Di, Qin Li, Xianjing |
author_role |
author |
author2 |
Yang, Xue Yan, Wenchao Li, Rui Ye, Qian You, Linjun Xie, Wenhao Mo, Kun Fu, Ruifeng Wang, Yanxiang Chen, Yufei Hou, Hui Yang, Yong Birnbaumer, Lutz Di, Qin Li, Xianjing |
author2_role |
author author author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
15-D-PGJ2 MACROPHAGE PPARΓ SEPSIS THYMOPENTIN |
topic |
15-D-PGJ2 MACROPHAGE PPARΓ SEPSIS THYMOPENTIN |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Sepsis, a systemic inflammatory response syndrome (SIRS) caused by infection, is a major public health concern with limited therapeutic options. Infection disturbs the homeostasis of host, resulting in excessive inflammation and immune suppression. This has prompted the clinical use of immunomodulators to balance host response as an alternative therapeutic strategy. Here, we report that Thymopentin (TP5), a synthetic immunomodulator pentapeptide (Arg-Lys-Asp-Val-Tyr) with an excellent safety profile in the clinic, protects mice against cecal ligation and puncture (CLP)-induced sepsis, as shown by improved survival rate, decreased level of pro-inflammatory cytokines and reduced ratios of macrophages and neutrophils in spleen and peritoneum. Regarding mechanism, TP5 changed the characteristics of LPS-stimulated macrophages by increasing the production of 15-deoxy-Δ12,14-prostaglandin J2 (15-d-PGJ2). In addition, the improved effect of TP5 on survival rates was abolished by the peroxisome proliferator-activated receptor γ (PPARγ) antagonist GW9662. Our results uncover the mechanism of the TP5 protective effects on CLP-induced sepsis and shed light on the development of TP5 as a therapeutic strategy for lethal systemic inflammatory disorders. Fil: Zhang, Ye. China Pharmaceutical University; China Fil: Yang, Xue. China Pharmaceutical University; China Fil: Yan, Wenchao. China Pharmaceutical University; China Fil: Li, Rui. China Pharmaceutical University; China Fil: Ye, Qian. China Pharmaceutical University; China Fil: You, Linjun. China Pharmaceutical University; China Fil: Xie, Wenhao. China Pharmaceutical University; China Fil: Mo, Kun. China Pharmaceutical University; China Fil: Fu, Ruifeng. China Pharmaceutical University; China Fil: Wang, Yanxiang. China Pharmaceutical University; China Fil: Chen, Yufei. China Pharmaceutical University; China Fil: Hou, Hui. China Pharmaceutical University; China Fil: Yang, Yong. China Pharmaceutical University; China Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina Fil: Di, Qin. Nanjing Sport Institute; China Fil: Li, Xianjing. China Pharmaceutical University; China |
description |
Sepsis, a systemic inflammatory response syndrome (SIRS) caused by infection, is a major public health concern with limited therapeutic options. Infection disturbs the homeostasis of host, resulting in excessive inflammation and immune suppression. This has prompted the clinical use of immunomodulators to balance host response as an alternative therapeutic strategy. Here, we report that Thymopentin (TP5), a synthetic immunomodulator pentapeptide (Arg-Lys-Asp-Val-Tyr) with an excellent safety profile in the clinic, protects mice against cecal ligation and puncture (CLP)-induced sepsis, as shown by improved survival rate, decreased level of pro-inflammatory cytokines and reduced ratios of macrophages and neutrophils in spleen and peritoneum. Regarding mechanism, TP5 changed the characteristics of LPS-stimulated macrophages by increasing the production of 15-deoxy-Δ12,14-prostaglandin J2 (15-d-PGJ2). In addition, the improved effect of TP5 on survival rates was abolished by the peroxisome proliferator-activated receptor γ (PPARγ) antagonist GW9662. Our results uncover the mechanism of the TP5 protective effects on CLP-induced sepsis and shed light on the development of TP5 as a therapeutic strategy for lethal systemic inflammatory disorders. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/140666 Zhang, Ye; Yang, Xue; Yan, Wenchao; Li, Rui; Ye, Qian; et al.; Thymopentin improves the survival of septic mice by promoting the production of 15-deoxy-prostaglandin J2 and activating the PPARγ signaling pathway; Federation of American Societies for Experimental Biology; FASEB Journal; 34; 9; 9-2020; 11772-11785 0892-6638 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/140666 |
identifier_str_mv |
Zhang, Ye; Yang, Xue; Yan, Wenchao; Li, Rui; Ye, Qian; et al.; Thymopentin improves the survival of septic mice by promoting the production of 15-deoxy-prostaglandin J2 and activating the PPARγ signaling pathway; Federation of American Societies for Experimental Biology; FASEB Journal; 34; 9; 9-2020; 11772-11785 0892-6638 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1096/fj.202000467R info:eu-repo/semantics/altIdentifier/url/https://faseb.onlinelibrary.wiley.com/doi/10.1096/fj.202000467R |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Federation of American Societies for Experimental Biology |
publisher.none.fl_str_mv |
Federation of American Societies for Experimental Biology |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269909359263744 |
score |
13.13397 |