Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype

Autores
Rostoker, Ran; Yaseen, Hiba; Schif Zuck, Sagie; Lichtenstein, Rachel G.; Rabinovich, Gabriel Adrián; Amiram, Ariel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
During the resolution of inflammation macrophages undergo functional changes upon exposure to pro-resolving agents in their microenvironment. Primarily, engulfment of apoptotic polymorphonuclear (PMN) cells promotes conversion of macrophages toward a pro-resolving phenotype characterized by reduced CD11b expression. These macrophages are not phagocytic, do not respond to TLR ligands, and express relatively high levels of the pro-resolving enzyme 12/15-lipoxygenase (LO). Here, we report that the immuno-regulatory lectin galectin-1 is selectively expressed by CD11bhigh, but not CD11blow macrophages. Upon exposure in vivo and ex vivo, galectin-1 directly promoted macrophage conversion from a CD11bhigh to a CD11blow phenotype and up-regulated the expression and activity of 12/15-LO. Moreover, galectin-1 treatment in vivo promoted the loss of phagocytic capacity (efferocytic satiation) in peritoneal macrophages and down-regulated secretion of TNF-α, IL-1β, and IL-10 upon LPS exposure. Our results suggest that galectin-1 could be an essential mediator in the control of macrophage function during the resolution of inflammation.
Fil: Rostoker, Ran. University of Haifa. Faculty of Natural Sciences. Departament of Biology; Israel
Fil: Yaseen, Hiba. University of Haifa. Faculty of Natural Sciences. Departament of Biology; Israel
Fil: Schif Zuck, Sagie. University of Haifa. Faculty of Natural Sciences. Departament of Biology; Israel
Fil: Lichtenstein, Rachel G.. Ben Gurion University of the Negev; Israel
Fil: Rabinovich, Gabriel Adrián. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Quimica Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Amiram, Ariel. University of Haifa. Faculty of Natural Sciences. Departament of Biology; Israel
Materia
15-LIPOXYGENASE
EFFEROCYTOSIS
GALECTIN-1
MACROPHAGES
RESOLUTION OF INFLAMMATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2354
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/2354
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotypeRostoker, RanYaseen, HibaSchif Zuck, SagieLichtenstein, Rachel G.Rabinovich, Gabriel AdriánAmiram, Ariel15-LIPOXYGENASEEFFEROCYTOSISGALECTIN-1MACROPHAGESRESOLUTION OF INFLAMMATIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3During the resolution of inflammation macrophages undergo functional changes upon exposure to pro-resolving agents in their microenvironment. Primarily, engulfment of apoptotic polymorphonuclear (PMN) cells promotes conversion of macrophages toward a pro-resolving phenotype characterized by reduced CD11b expression. These macrophages are not phagocytic, do not respond to TLR ligands, and express relatively high levels of the pro-resolving enzyme 12/15-lipoxygenase (LO). Here, we report that the immuno-regulatory lectin galectin-1 is selectively expressed by CD11bhigh, but not CD11blow macrophages. Upon exposure in vivo and ex vivo, galectin-1 directly promoted macrophage conversion from a CD11bhigh to a CD11blow phenotype and up-regulated the expression and activity of 12/15-LO. Moreover, galectin-1 treatment in vivo promoted the loss of phagocytic capacity (efferocytic satiation) in peritoneal macrophages and down-regulated secretion of TNF-α, IL-1β, and IL-10 upon LPS exposure. Our results suggest that galectin-1 could be an essential mediator in the control of macrophage function during the resolution of inflammation.Fil: Rostoker, Ran. University of Haifa. Faculty of Natural Sciences. Departament of Biology; IsraelFil: Yaseen, Hiba. University of Haifa. Faculty of Natural Sciences. Departament of Biology; IsraelFil: Schif Zuck, Sagie. University of Haifa. Faculty of Natural Sciences. Departament of Biology; IsraelFil: Lichtenstein, Rachel G.. Ben Gurion University of the Negev; IsraelFil: Rabinovich, Gabriel Adrián. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Quimica Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Amiram, Ariel. University of Haifa. Faculty of Natural Sciences. Departament of Biology; IsraelElsevier Science Inc2013-08-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2354Rostoker, Ran; Yaseen, Hiba; Schif Zuck, Sagie; Lichtenstein, Rachel G.; Rabinovich, Gabriel Adrián; et al.; Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype; Elsevier Science Inc; Prostaglandins; 107; 14-8-2013; 85-941098-8823eng4th European Workshop on Lipid Mediatorsinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.prostaglandins.2013.08.001info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S109888231300066Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:04:29Zoai:ri.conicet.gov.ar:11336/2354instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:04:29.654CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype
title Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype
spellingShingle Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype
Rostoker, Ran
15-LIPOXYGENASE
EFFEROCYTOSIS
GALECTIN-1
MACROPHAGES
RESOLUTION OF INFLAMMATION
title_short Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype
title_full Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype
title_fullStr Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype
title_full_unstemmed Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype
title_sort Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype
dc.creator.none.fl_str_mv Rostoker, Ran
Yaseen, Hiba
Schif Zuck, Sagie
Lichtenstein, Rachel G.
Rabinovich, Gabriel Adrián
Amiram, Ariel
author Rostoker, Ran
author_facet Rostoker, Ran
Yaseen, Hiba
Schif Zuck, Sagie
Lichtenstein, Rachel G.
Rabinovich, Gabriel Adrián
Amiram, Ariel
author_role author
author2 Yaseen, Hiba
Schif Zuck, Sagie
Lichtenstein, Rachel G.
Rabinovich, Gabriel Adrián
Amiram, Ariel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv 15-LIPOXYGENASE
EFFEROCYTOSIS
GALECTIN-1
MACROPHAGES
RESOLUTION OF INFLAMMATION
topic 15-LIPOXYGENASE
EFFEROCYTOSIS
GALECTIN-1
MACROPHAGES
RESOLUTION OF INFLAMMATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv During the resolution of inflammation macrophages undergo functional changes upon exposure to pro-resolving agents in their microenvironment. Primarily, engulfment of apoptotic polymorphonuclear (PMN) cells promotes conversion of macrophages toward a pro-resolving phenotype characterized by reduced CD11b expression. These macrophages are not phagocytic, do not respond to TLR ligands, and express relatively high levels of the pro-resolving enzyme 12/15-lipoxygenase (LO). Here, we report that the immuno-regulatory lectin galectin-1 is selectively expressed by CD11bhigh, but not CD11blow macrophages. Upon exposure in vivo and ex vivo, galectin-1 directly promoted macrophage conversion from a CD11bhigh to a CD11blow phenotype and up-regulated the expression and activity of 12/15-LO. Moreover, galectin-1 treatment in vivo promoted the loss of phagocytic capacity (efferocytic satiation) in peritoneal macrophages and down-regulated secretion of TNF-α, IL-1β, and IL-10 upon LPS exposure. Our results suggest that galectin-1 could be an essential mediator in the control of macrophage function during the resolution of inflammation.
Fil: Rostoker, Ran. University of Haifa. Faculty of Natural Sciences. Departament of Biology; Israel
Fil: Yaseen, Hiba. University of Haifa. Faculty of Natural Sciences. Departament of Biology; Israel
Fil: Schif Zuck, Sagie. University of Haifa. Faculty of Natural Sciences. Departament of Biology; Israel
Fil: Lichtenstein, Rachel G.. Ben Gurion University of the Negev; Israel
Fil: Rabinovich, Gabriel Adrián. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Quimica Biologica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Amiram, Ariel. University of Haifa. Faculty of Natural Sciences. Departament of Biology; Israel
description During the resolution of inflammation macrophages undergo functional changes upon exposure to pro-resolving agents in their microenvironment. Primarily, engulfment of apoptotic polymorphonuclear (PMN) cells promotes conversion of macrophages toward a pro-resolving phenotype characterized by reduced CD11b expression. These macrophages are not phagocytic, do not respond to TLR ligands, and express relatively high levels of the pro-resolving enzyme 12/15-lipoxygenase (LO). Here, we report that the immuno-regulatory lectin galectin-1 is selectively expressed by CD11bhigh, but not CD11blow macrophages. Upon exposure in vivo and ex vivo, galectin-1 directly promoted macrophage conversion from a CD11bhigh to a CD11blow phenotype and up-regulated the expression and activity of 12/15-LO. Moreover, galectin-1 treatment in vivo promoted the loss of phagocytic capacity (efferocytic satiation) in peritoneal macrophages and down-regulated secretion of TNF-α, IL-1β, and IL-10 upon LPS exposure. Our results suggest that galectin-1 could be an essential mediator in the control of macrophage function during the resolution of inflammation.
publishDate 2013
dc.date.none.fl_str_mv 2013-08-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2354
Rostoker, Ran; Yaseen, Hiba; Schif Zuck, Sagie; Lichtenstein, Rachel G.; Rabinovich, Gabriel Adrián; et al.; Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype; Elsevier Science Inc; Prostaglandins; 107; 14-8-2013; 85-94
1098-8823
url http://hdl.handle.net/11336/2354
identifier_str_mv Rostoker, Ran; Yaseen, Hiba; Schif Zuck, Sagie; Lichtenstein, Rachel G.; Rabinovich, Gabriel Adrián; et al.; Galectin-1 induces 12/15-lipoxygenase expression in murine macrophages and favors their conversion toward a pro-resolving phenotype; Elsevier Science Inc; Prostaglandins; 107; 14-8-2013; 85-94
1098-8823
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 4th European Workshop on Lipid Mediators
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.prostaglandins.2013.08.001
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S109888231300066X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269858087043072
score 13.13397

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