Neurogenin 3 mediates sex chromosome effects on the generation of sex differences in hypothalamic neuronal development

Autores
Scerbo Jaureguiberry, Maria Julia; Freire Regatillo, Alejandra; Cisternas, Carla Daniela; Brunotto, Mabel Noemí; Arevalo, Maria Angeles; Garcia Segura, Luis Miguel; Cambiasso, Maria Julia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The organizational action of testosterone during critical periods of development is the cause of numerous sex differences in the brain. However, sex differences in neuritogenesis have been detected in primary neuronal hypothalamic cultures prepared before the peak of testosterone production by fetal testis. In the present study we assessed the hypothesis of that cell-autonomous action of sex chromosomes can differentially regulate the expression of the neuritogenic gene neurogenin 3 (Ngn3) in male and female hypothalamic neurons, generating sex differences in neuronal development. Neuronal cultures were prepared from male and female E14 mouse hypothalami, before the fetal peak of testosterone. Female neurons showed enhanced neuritogenesis and higher expression of Ngn3 than male neurons. The silencing of Ngn3 abolished sex differences in neuritogenesis, decreasing the differentiation of female neurons.The sex difference in Ngn3 expression was determined by sex chromosomes, as demonstrated using the four core genotypes mouse model, in which a spontaneous deletion of the testis-determining gene Sry from theY chromosome was combined with the insertion of the Sry gene onto an autosome. In addition, the expression of Ngn3, which is also known to mediate the neuritogenic actions of estradiol, was increased in the cultures treated with the hormone, but only in those from male embryos. Furthermore, the hormone reversed the sex differences in neuritogenesis promoting the differentiation of male neurons. These findings indicate that Ngn3 mediates both cell-autonomous actions of sex chromosomes and hormonal effects on neuritogenesis.
Fil: Scerbo Jaureguiberry, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Freire Regatillo, Alejandra. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; España
Fil: Cisternas, Carla Daniela. Universidad Nacional de Cordoba. Facultad de Odontologia. Departamento de Biologia Bucal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Brunotto, Mabel Noemí. Universidad Nacional de Cordoba. Facultad de Odontologia. Departamento de Biologia Bucal; Argentina
Fil: Arevalo, Maria Angeles. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; España
Fil: Garcia Segura, Luis Miguel. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; España
Fil: Cambiasso, Maria Julia. Universidad Nacional de Cordoba. Facultad de Odontologia. Departamento de Biologia Bucal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Materia
ESTRADIOL
HYPOTHALAMIC NEURONS
NEURITOGENESIS
NGN3
SEX CHROMOSOMES
SEX DIFFERENCES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/185625
Acceder
id CONICETDig_b228827d1ece065dc29bfcb4d764d85b
oai_identifier_str oai:ri.conicet.gov.ar:11336/185625
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Neurogenin 3 mediates sex chromosome effects on the generation of sex differences in hypothalamic neuronal developmentScerbo Jaureguiberry, Maria JuliaFreire Regatillo, AlejandraCisternas, Carla DanielaBrunotto, Mabel NoemíArevalo, Maria AngelesGarcia Segura, Luis MiguelCambiasso, Maria JuliaESTRADIOLHYPOTHALAMIC NEURONSNEURITOGENESISNGN3SEX CHROMOSOMESSEX DIFFERENCEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The organizational action of testosterone during critical periods of development is the cause of numerous sex differences in the brain. However, sex differences in neuritogenesis have been detected in primary neuronal hypothalamic cultures prepared before the peak of testosterone production by fetal testis. In the present study we assessed the hypothesis of that cell-autonomous action of sex chromosomes can differentially regulate the expression of the neuritogenic gene neurogenin 3 (Ngn3) in male and female hypothalamic neurons, generating sex differences in neuronal development. Neuronal cultures were prepared from male and female E14 mouse hypothalami, before the fetal peak of testosterone. Female neurons showed enhanced neuritogenesis and higher expression of Ngn3 than male neurons. The silencing of Ngn3 abolished sex differences in neuritogenesis, decreasing the differentiation of female neurons.The sex difference in Ngn3 expression was determined by sex chromosomes, as demonstrated using the four core genotypes mouse model, in which a spontaneous deletion of the testis-determining gene Sry from theY chromosome was combined with the insertion of the Sry gene onto an autosome. In addition, the expression of Ngn3, which is also known to mediate the neuritogenic actions of estradiol, was increased in the cultures treated with the hormone, but only in those from male embryos. Furthermore, the hormone reversed the sex differences in neuritogenesis promoting the differentiation of male neurons. These findings indicate that Ngn3 mediates both cell-autonomous actions of sex chromosomes and hormonal effects on neuritogenesis.Fil: Scerbo Jaureguiberry, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Freire Regatillo, Alejandra. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Cisternas, Carla Daniela. Universidad Nacional de Cordoba. Facultad de Odontologia. Departamento de Biologia Bucal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Brunotto, Mabel Noemí. Universidad Nacional de Cordoba. Facultad de Odontologia. Departamento de Biologia Bucal; ArgentinaFil: Arevalo, Maria Angeles. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Garcia Segura, Luis Miguel. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Cambiasso, Maria Julia. Universidad Nacional de Cordoba. Facultad de Odontologia. Departamento de Biologia Bucal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFrontiers Research Foundation2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/185625Scerbo Jaureguiberry, Maria Julia; Freire Regatillo, Alejandra; Cisternas, Carla Daniela; Brunotto, Mabel Noemí; Arevalo, Maria Angeles; et al.; Neurogenin 3 mediates sex chromosome effects on the generation of sex differences in hypothalamic neuronal development; Frontiers Research Foundation; Frontiers in Cellular Neuroscience; 8; 188; 7-2014; 1-131662-5102CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fncel.2014.00188/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fncel.2014.00188info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:45:04Zoai:ri.conicet.gov.ar:11336/185625instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:45:05.117CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Neurogenin 3 mediates sex chromosome effects on the generation of sex differences in hypothalamic neuronal development
title Neurogenin 3 mediates sex chromosome effects on the generation of sex differences in hypothalamic neuronal development
spellingShingle Neurogenin 3 mediates sex chromosome effects on the generation of sex differences in hypothalamic neuronal development
Scerbo Jaureguiberry, Maria Julia
ESTRADIOL
HYPOTHALAMIC NEURONS
NEURITOGENESIS
NGN3
SEX CHROMOSOMES
SEX DIFFERENCES
title_short Neurogenin 3 mediates sex chromosome effects on the generation of sex differences in hypothalamic neuronal development
title_full Neurogenin 3 mediates sex chromosome effects on the generation of sex differences in hypothalamic neuronal development
title_fullStr Neurogenin 3 mediates sex chromosome effects on the generation of sex differences in hypothalamic neuronal development
title_full_unstemmed Neurogenin 3 mediates sex chromosome effects on the generation of sex differences in hypothalamic neuronal development
title_sort Neurogenin 3 mediates sex chromosome effects on the generation of sex differences in hypothalamic neuronal development
dc.creator.none.fl_str_mv Scerbo Jaureguiberry, Maria Julia
Freire Regatillo, Alejandra
Cisternas, Carla Daniela
Brunotto, Mabel Noemí
Arevalo, Maria Angeles
Garcia Segura, Luis Miguel
Cambiasso, Maria Julia
author Scerbo Jaureguiberry, Maria Julia
author_facet Scerbo Jaureguiberry, Maria Julia
Freire Regatillo, Alejandra
Cisternas, Carla Daniela
Brunotto, Mabel Noemí
Arevalo, Maria Angeles
Garcia Segura, Luis Miguel
Cambiasso, Maria Julia
author_role author
author2 Freire Regatillo, Alejandra
Cisternas, Carla Daniela
Brunotto, Mabel Noemí
Arevalo, Maria Angeles
Garcia Segura, Luis Miguel
Cambiasso, Maria Julia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ESTRADIOL
HYPOTHALAMIC NEURONS
NEURITOGENESIS
NGN3
SEX CHROMOSOMES
SEX DIFFERENCES
topic ESTRADIOL
HYPOTHALAMIC NEURONS
NEURITOGENESIS
NGN3
SEX CHROMOSOMES
SEX DIFFERENCES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The organizational action of testosterone during critical periods of development is the cause of numerous sex differences in the brain. However, sex differences in neuritogenesis have been detected in primary neuronal hypothalamic cultures prepared before the peak of testosterone production by fetal testis. In the present study we assessed the hypothesis of that cell-autonomous action of sex chromosomes can differentially regulate the expression of the neuritogenic gene neurogenin 3 (Ngn3) in male and female hypothalamic neurons, generating sex differences in neuronal development. Neuronal cultures were prepared from male and female E14 mouse hypothalami, before the fetal peak of testosterone. Female neurons showed enhanced neuritogenesis and higher expression of Ngn3 than male neurons. The silencing of Ngn3 abolished sex differences in neuritogenesis, decreasing the differentiation of female neurons.The sex difference in Ngn3 expression was determined by sex chromosomes, as demonstrated using the four core genotypes mouse model, in which a spontaneous deletion of the testis-determining gene Sry from theY chromosome was combined with the insertion of the Sry gene onto an autosome. In addition, the expression of Ngn3, which is also known to mediate the neuritogenic actions of estradiol, was increased in the cultures treated with the hormone, but only in those from male embryos. Furthermore, the hormone reversed the sex differences in neuritogenesis promoting the differentiation of male neurons. These findings indicate that Ngn3 mediates both cell-autonomous actions of sex chromosomes and hormonal effects on neuritogenesis.
Fil: Scerbo Jaureguiberry, Maria Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Freire Regatillo, Alejandra. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; España
Fil: Cisternas, Carla Daniela. Universidad Nacional de Cordoba. Facultad de Odontologia. Departamento de Biologia Bucal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Brunotto, Mabel Noemí. Universidad Nacional de Cordoba. Facultad de Odontologia. Departamento de Biologia Bucal; Argentina
Fil: Arevalo, Maria Angeles. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; España
Fil: Garcia Segura, Luis Miguel. Instituto Cajal; España. Consejo Superior de Investigaciones Científicas; España
Fil: Cambiasso, Maria Julia. Universidad Nacional de Cordoba. Facultad de Odontologia. Departamento de Biologia Bucal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
description The organizational action of testosterone during critical periods of development is the cause of numerous sex differences in the brain. However, sex differences in neuritogenesis have been detected in primary neuronal hypothalamic cultures prepared before the peak of testosterone production by fetal testis. In the present study we assessed the hypothesis of that cell-autonomous action of sex chromosomes can differentially regulate the expression of the neuritogenic gene neurogenin 3 (Ngn3) in male and female hypothalamic neurons, generating sex differences in neuronal development. Neuronal cultures were prepared from male and female E14 mouse hypothalami, before the fetal peak of testosterone. Female neurons showed enhanced neuritogenesis and higher expression of Ngn3 than male neurons. The silencing of Ngn3 abolished sex differences in neuritogenesis, decreasing the differentiation of female neurons.The sex difference in Ngn3 expression was determined by sex chromosomes, as demonstrated using the four core genotypes mouse model, in which a spontaneous deletion of the testis-determining gene Sry from theY chromosome was combined with the insertion of the Sry gene onto an autosome. In addition, the expression of Ngn3, which is also known to mediate the neuritogenic actions of estradiol, was increased in the cultures treated with the hormone, but only in those from male embryos. Furthermore, the hormone reversed the sex differences in neuritogenesis promoting the differentiation of male neurons. These findings indicate that Ngn3 mediates both cell-autonomous actions of sex chromosomes and hormonal effects on neuritogenesis.
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/185625
Scerbo Jaureguiberry, Maria Julia; Freire Regatillo, Alejandra; Cisternas, Carla Daniela; Brunotto, Mabel Noemí; Arevalo, Maria Angeles; et al.; Neurogenin 3 mediates sex chromosome effects on the generation of sex differences in hypothalamic neuronal development; Frontiers Research Foundation; Frontiers in Cellular Neuroscience; 8; 188; 7-2014; 1-13
1662-5102
CONICET Digital
CONICET
url http://hdl.handle.net/11336/185625
identifier_str_mv Scerbo Jaureguiberry, Maria Julia; Freire Regatillo, Alejandra; Cisternas, Carla Daniela; Brunotto, Mabel Noemí; Arevalo, Maria Angeles; et al.; Neurogenin 3 mediates sex chromosome effects on the generation of sex differences in hypothalamic neuronal development; Frontiers Research Foundation; Frontiers in Cellular Neuroscience; 8; 188; 7-2014; 1-13
1662-5102
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fncel.2014.00188/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fncel.2014.00188
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Research Foundation
publisher.none.fl_str_mv Frontiers Research Foundation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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