RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli

Autores
Guo, Yunxue; Quiroga, Cecilia; Chen, Qin; McAnulty, Michael J.; Benedik, Michael J.; Wood, Thomas K.; Wang, Xiaoxue
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
For toxin/antitoxin (TA) systems, no toxin has been identified that functions by cleaving DNA. Here, we demonstrate that RalR and RalA of the cryptic prophage rac form a type I TA pair in which the antitoxin RNA is a trans-encoded small RNA with 16 nucleotides of complementarity to the toxin mRNA. We suggest the newly discovered antitoxin gene be named ralA for RalR antitoxin. Toxin RalR functions as a non-specific endonuclease that cleaves methylated and unmethylated DNA. The RNA chaperone Hfq is required for RalA antitoxin activity and appears to stabilize RalA. Also, RalR/RalA is beneficial to the Escherichia coli host for responding to the antibiotic fosfomycin. Hence, our results indicate that cryptic prophage genes can be functionally divergent from their active phage counterparts after integration into the host genome.
Fil: Guo, Yunxue. Chinese Academy Of Sciences; China
Fil: Quiroga, Cecilia. State University of Pennsylvania; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Chen, Qin. State University of Pennsylvania; Estados Unidos
Fil: McAnulty, Michael J.. State University of Pennsylvania; Estados Unidos
Fil: Benedik, Michael J.. Texas A&M University; Estados Unidos
Fil: Wood, Thomas K.. State University of Pennsylvania; Estados Unidos
Fil: Wang, Xiaoxue. Chinese Academy of Sciences; República de China
Materia
Toxin/antitoxin
rac prophage,
type I TA system
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84740
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/84740
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coliGuo, YunxueQuiroga, CeciliaChen, QinMcAnulty, Michael J.Benedik, Michael J.Wood, Thomas K.Wang, XiaoxueToxin/antitoxinrac prophage,type I TA systemhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1For toxin/antitoxin (TA) systems, no toxin has been identified that functions by cleaving DNA. Here, we demonstrate that RalR and RalA of the cryptic prophage rac form a type I TA pair in which the antitoxin RNA is a trans-encoded small RNA with 16 nucleotides of complementarity to the toxin mRNA. We suggest the newly discovered antitoxin gene be named ralA for RalR antitoxin. Toxin RalR functions as a non-specific endonuclease that cleaves methylated and unmethylated DNA. The RNA chaperone Hfq is required for RalA antitoxin activity and appears to stabilize RalA. Also, RalR/RalA is beneficial to the Escherichia coli host for responding to the antibiotic fosfomycin. Hence, our results indicate that cryptic prophage genes can be functionally divergent from their active phage counterparts after integration into the host genome.Fil: Guo, Yunxue. Chinese Academy Of Sciences; ChinaFil: Quiroga, Cecilia. State University of Pennsylvania; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Chen, Qin. State University of Pennsylvania; Estados UnidosFil: McAnulty, Michael J.. State University of Pennsylvania; Estados UnidosFil: Benedik, Michael J.. Texas A&M University; Estados UnidosFil: Wood, Thomas K.. State University of Pennsylvania; Estados UnidosFil: Wang, Xiaoxue. Chinese Academy of Sciences; República de ChinaOxford University Press2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84740Guo, Yunxue; Quiroga, Cecilia; Chen, Qin; McAnulty, Michael J.; Benedik, Michael J.; et al.; RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli; Oxford University Press; Nucleic Acids Research; 42; 10; 6-2014; 6448-64620305-10481362-4962CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/nar/gku279info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/nar/article/42/10/6448/2435759info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:18:30Zoai:ri.conicet.gov.ar:11336/84740instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:18:30.977CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli
title RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli
spellingShingle RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli
Guo, Yunxue
Toxin/antitoxin
rac prophage,
type I TA system
title_short RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli
title_full RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli
title_fullStr RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli
title_full_unstemmed RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli
title_sort RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli
dc.creator.none.fl_str_mv Guo, Yunxue
Quiroga, Cecilia
Chen, Qin
McAnulty, Michael J.
Benedik, Michael J.
Wood, Thomas K.
Wang, Xiaoxue
author Guo, Yunxue
author_facet Guo, Yunxue
Quiroga, Cecilia
Chen, Qin
McAnulty, Michael J.
Benedik, Michael J.
Wood, Thomas K.
Wang, Xiaoxue
author_role author
author2 Quiroga, Cecilia
Chen, Qin
McAnulty, Michael J.
Benedik, Michael J.
Wood, Thomas K.
Wang, Xiaoxue
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Toxin/antitoxin
rac prophage,
type I TA system
topic Toxin/antitoxin
rac prophage,
type I TA system
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv For toxin/antitoxin (TA) systems, no toxin has been identified that functions by cleaving DNA. Here, we demonstrate that RalR and RalA of the cryptic prophage rac form a type I TA pair in which the antitoxin RNA is a trans-encoded small RNA with 16 nucleotides of complementarity to the toxin mRNA. We suggest the newly discovered antitoxin gene be named ralA for RalR antitoxin. Toxin RalR functions as a non-specific endonuclease that cleaves methylated and unmethylated DNA. The RNA chaperone Hfq is required for RalA antitoxin activity and appears to stabilize RalA. Also, RalR/RalA is beneficial to the Escherichia coli host for responding to the antibiotic fosfomycin. Hence, our results indicate that cryptic prophage genes can be functionally divergent from their active phage counterparts after integration into the host genome.
Fil: Guo, Yunxue. Chinese Academy Of Sciences; China
Fil: Quiroga, Cecilia. State University of Pennsylvania; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Chen, Qin. State University of Pennsylvania; Estados Unidos
Fil: McAnulty, Michael J.. State University of Pennsylvania; Estados Unidos
Fil: Benedik, Michael J.. Texas A&M University; Estados Unidos
Fil: Wood, Thomas K.. State University of Pennsylvania; Estados Unidos
Fil: Wang, Xiaoxue. Chinese Academy of Sciences; República de China
description For toxin/antitoxin (TA) systems, no toxin has been identified that functions by cleaving DNA. Here, we demonstrate that RalR and RalA of the cryptic prophage rac form a type I TA pair in which the antitoxin RNA is a trans-encoded small RNA with 16 nucleotides of complementarity to the toxin mRNA. We suggest the newly discovered antitoxin gene be named ralA for RalR antitoxin. Toxin RalR functions as a non-specific endonuclease that cleaves methylated and unmethylated DNA. The RNA chaperone Hfq is required for RalA antitoxin activity and appears to stabilize RalA. Also, RalR/RalA is beneficial to the Escherichia coli host for responding to the antibiotic fosfomycin. Hence, our results indicate that cryptic prophage genes can be functionally divergent from their active phage counterparts after integration into the host genome.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84740
Guo, Yunxue; Quiroga, Cecilia; Chen, Qin; McAnulty, Michael J.; Benedik, Michael J.; et al.; RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli; Oxford University Press; Nucleic Acids Research; 42; 10; 6-2014; 6448-6462
0305-1048
1362-4962
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84740
identifier_str_mv Guo, Yunxue; Quiroga, Cecilia; Chen, Qin; McAnulty, Michael J.; Benedik, Michael J.; et al.; RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli; Oxford University Press; Nucleic Acids Research; 42; 10; 6-2014; 6448-6462
0305-1048
1362-4962
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/nar/gku279
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/nar/article/42/10/6448/2435759
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781648814735360
score 12.982451

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