Hyaluronate Protects From Benzalkonium Chloride-Induced Ocular Surface Toxicity

Autores
Vereertbrugghen, Alexia; Pizzano, Manuela; Sabbione, Florencia; del Papa, Melina S.; Rodríguez, Giselle; Passerini, María Silvia; Galletti, Jeremías Gastón
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Purpose: The purpose of this study was to investigate the effect of sodium hyaluronate (SH) on benzalkonium chloride (BAK)-induced toxicity in the ocular surface epithelium and corneal nerves. Methods: Ocular surface epithelial cells from Balb/c mice were cultured with 0.1% to 0.4% SH and 0.001% to 0.01% BAK and their metabolic activity, viability, and wound repair capacity were assessed in vitro. Following a controlled corneal wound, reepithelialization and recovery of epithelial barrier function and mechanosensitivity were measured in Balb/c mice treated with 0.4% SH 3 times/day and 0.01% BAK twice daily for 3 weeks. Nerve morphology was assessed by confocal microscopy of corneal whole mounts. Results: Whereas BAK exposure reduced metabolic activity, viability, and wound repair ability of ocular epithelial cells in vitro, pretreatment with SH ameliorated BAK toxicity in a concentration-dependent manner. The highest SH concentration partially reversed the effects of 0.01% BAK in vitro and increased the corneal healing rate of BAK-exposed mice. Although all corneal wounds closed after 4 days, continuous SH treatment improved corneal barrier dysfunction 18 days after wounding and accelerated the recovery of corneal mechanical sensitivity to baseline levels in BAK-exposed mice. SH treatment also increased corneal nerve density in the wounded area after 3 weeks. Conclusions: SH mitigates BAK-associated ocular epithelial and neurotoxicity in a concentration-dependent manner. Translational Relevance: Commercially available, high-concentration SH formulations may have added benefits in treating BAK-associated ocular surface toxicity.
Fil: Vereertbrugghen, Alexia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Pizzano, Manuela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Sabbione, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: del Papa, Melina S.. Laboratorios Poen Sociedad Anonima Unipersonal .; Argentina
Fil: Rodríguez, Giselle. Laboratorios Poen Sociedad Anonima Unipersonal .; Argentina
Fil: Passerini, María Silvia. Laboratorios Poen Sociedad Anonima Unipersonal .; Argentina
Fil: Galletti, Jeremías Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Materia
Sodium hyaluronate
Benzalkonium chloride
Ocular surface epithelium
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/265111
Acceder
id CONICETDig_b14f73a1870aabab5a36ff61609a202c
oai_identifier_str oai:ri.conicet.gov.ar:11336/265111
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Hyaluronate Protects From Benzalkonium Chloride-Induced Ocular Surface ToxicityVereertbrugghen, AlexiaPizzano, ManuelaSabbione, Florenciadel Papa, Melina S.Rodríguez, GisellePasserini, María SilviaGalletti, Jeremías GastónSodium hyaluronateBenzalkonium chlorideOcular surface epitheliumhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Purpose: The purpose of this study was to investigate the effect of sodium hyaluronate (SH) on benzalkonium chloride (BAK)-induced toxicity in the ocular surface epithelium and corneal nerves. Methods: Ocular surface epithelial cells from Balb/c mice were cultured with 0.1% to 0.4% SH and 0.001% to 0.01% BAK and their metabolic activity, viability, and wound repair capacity were assessed in vitro. Following a controlled corneal wound, reepithelialization and recovery of epithelial barrier function and mechanosensitivity were measured in Balb/c mice treated with 0.4% SH 3 times/day and 0.01% BAK twice daily for 3 weeks. Nerve morphology was assessed by confocal microscopy of corneal whole mounts. Results: Whereas BAK exposure reduced metabolic activity, viability, and wound repair ability of ocular epithelial cells in vitro, pretreatment with SH ameliorated BAK toxicity in a concentration-dependent manner. The highest SH concentration partially reversed the effects of 0.01% BAK in vitro and increased the corneal healing rate of BAK-exposed mice. Although all corneal wounds closed after 4 days, continuous SH treatment improved corneal barrier dysfunction 18 days after wounding and accelerated the recovery of corneal mechanical sensitivity to baseline levels in BAK-exposed mice. SH treatment also increased corneal nerve density in the wounded area after 3 weeks. Conclusions: SH mitigates BAK-associated ocular epithelial and neurotoxicity in a concentration-dependent manner. Translational Relevance: Commercially available, high-concentration SH formulations may have added benefits in treating BAK-associated ocular surface toxicity.Fil: Vereertbrugghen, Alexia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Pizzano, Manuela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Sabbione, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: del Papa, Melina S.. Laboratorios Poen Sociedad Anonima Unipersonal .; ArgentinaFil: Rodríguez, Giselle. Laboratorios Poen Sociedad Anonima Unipersonal .; ArgentinaFil: Passerini, María Silvia. Laboratorios Poen Sociedad Anonima Unipersonal .; ArgentinaFil: Galletti, Jeremías Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaAssociation for Research in Vision and Ophthalmology2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/265111Vereertbrugghen, Alexia; Pizzano, Manuela; Sabbione, Florencia; del Papa, Melina S.; Rodríguez, Giselle; et al.; Hyaluronate Protects From Benzalkonium Chloride-Induced Ocular Surface Toxicity; Association for Research in Vision and Ophthalmology; Translational Vision Science & Technology; 13; 10; 10-2024; 1-152164-2591CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://tvst.arvojournals.org/article.aspx?articleid=2802155info:eu-repo/semantics/altIdentifier/doi/10.1167/tvst.13.10.31info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:29:46Zoai:ri.conicet.gov.ar:11336/265111instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:29:47.002CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Hyaluronate Protects From Benzalkonium Chloride-Induced Ocular Surface Toxicity
title Hyaluronate Protects From Benzalkonium Chloride-Induced Ocular Surface Toxicity
spellingShingle Hyaluronate Protects From Benzalkonium Chloride-Induced Ocular Surface Toxicity
Vereertbrugghen, Alexia
Sodium hyaluronate
Benzalkonium chloride
Ocular surface epithelium
title_short Hyaluronate Protects From Benzalkonium Chloride-Induced Ocular Surface Toxicity
title_full Hyaluronate Protects From Benzalkonium Chloride-Induced Ocular Surface Toxicity
title_fullStr Hyaluronate Protects From Benzalkonium Chloride-Induced Ocular Surface Toxicity
title_full_unstemmed Hyaluronate Protects From Benzalkonium Chloride-Induced Ocular Surface Toxicity
title_sort Hyaluronate Protects From Benzalkonium Chloride-Induced Ocular Surface Toxicity
dc.creator.none.fl_str_mv Vereertbrugghen, Alexia
Pizzano, Manuela
Sabbione, Florencia
del Papa, Melina S.
Rodríguez, Giselle
Passerini, María Silvia
Galletti, Jeremías Gastón
author Vereertbrugghen, Alexia
author_facet Vereertbrugghen, Alexia
Pizzano, Manuela
Sabbione, Florencia
del Papa, Melina S.
Rodríguez, Giselle
Passerini, María Silvia
Galletti, Jeremías Gastón
author_role author
author2 Pizzano, Manuela
Sabbione, Florencia
del Papa, Melina S.
Rodríguez, Giselle
Passerini, María Silvia
Galletti, Jeremías Gastón
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Sodium hyaluronate
Benzalkonium chloride
Ocular surface epithelium
topic Sodium hyaluronate
Benzalkonium chloride
Ocular surface epithelium
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Purpose: The purpose of this study was to investigate the effect of sodium hyaluronate (SH) on benzalkonium chloride (BAK)-induced toxicity in the ocular surface epithelium and corneal nerves. Methods: Ocular surface epithelial cells from Balb/c mice were cultured with 0.1% to 0.4% SH and 0.001% to 0.01% BAK and their metabolic activity, viability, and wound repair capacity were assessed in vitro. Following a controlled corneal wound, reepithelialization and recovery of epithelial barrier function and mechanosensitivity were measured in Balb/c mice treated with 0.4% SH 3 times/day and 0.01% BAK twice daily for 3 weeks. Nerve morphology was assessed by confocal microscopy of corneal whole mounts. Results: Whereas BAK exposure reduced metabolic activity, viability, and wound repair ability of ocular epithelial cells in vitro, pretreatment with SH ameliorated BAK toxicity in a concentration-dependent manner. The highest SH concentration partially reversed the effects of 0.01% BAK in vitro and increased the corneal healing rate of BAK-exposed mice. Although all corneal wounds closed after 4 days, continuous SH treatment improved corneal barrier dysfunction 18 days after wounding and accelerated the recovery of corneal mechanical sensitivity to baseline levels in BAK-exposed mice. SH treatment also increased corneal nerve density in the wounded area after 3 weeks. Conclusions: SH mitigates BAK-associated ocular epithelial and neurotoxicity in a concentration-dependent manner. Translational Relevance: Commercially available, high-concentration SH formulations may have added benefits in treating BAK-associated ocular surface toxicity.
Fil: Vereertbrugghen, Alexia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Pizzano, Manuela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Sabbione, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: del Papa, Melina S.. Laboratorios Poen Sociedad Anonima Unipersonal .; Argentina
Fil: Rodríguez, Giselle. Laboratorios Poen Sociedad Anonima Unipersonal .; Argentina
Fil: Passerini, María Silvia. Laboratorios Poen Sociedad Anonima Unipersonal .; Argentina
Fil: Galletti, Jeremías Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
description Purpose: The purpose of this study was to investigate the effect of sodium hyaluronate (SH) on benzalkonium chloride (BAK)-induced toxicity in the ocular surface epithelium and corneal nerves. Methods: Ocular surface epithelial cells from Balb/c mice were cultured with 0.1% to 0.4% SH and 0.001% to 0.01% BAK and their metabolic activity, viability, and wound repair capacity were assessed in vitro. Following a controlled corneal wound, reepithelialization and recovery of epithelial barrier function and mechanosensitivity were measured in Balb/c mice treated with 0.4% SH 3 times/day and 0.01% BAK twice daily for 3 weeks. Nerve morphology was assessed by confocal microscopy of corneal whole mounts. Results: Whereas BAK exposure reduced metabolic activity, viability, and wound repair ability of ocular epithelial cells in vitro, pretreatment with SH ameliorated BAK toxicity in a concentration-dependent manner. The highest SH concentration partially reversed the effects of 0.01% BAK in vitro and increased the corneal healing rate of BAK-exposed mice. Although all corneal wounds closed after 4 days, continuous SH treatment improved corneal barrier dysfunction 18 days after wounding and accelerated the recovery of corneal mechanical sensitivity to baseline levels in BAK-exposed mice. SH treatment also increased corneal nerve density in the wounded area after 3 weeks. Conclusions: SH mitigates BAK-associated ocular epithelial and neurotoxicity in a concentration-dependent manner. Translational Relevance: Commercially available, high-concentration SH formulations may have added benefits in treating BAK-associated ocular surface toxicity.
publishDate 2024
dc.date.none.fl_str_mv 2024-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/265111
Vereertbrugghen, Alexia; Pizzano, Manuela; Sabbione, Florencia; del Papa, Melina S.; Rodríguez, Giselle; et al.; Hyaluronate Protects From Benzalkonium Chloride-Induced Ocular Surface Toxicity; Association for Research in Vision and Ophthalmology; Translational Vision Science & Technology; 13; 10; 10-2024; 1-15
2164-2591
CONICET Digital
CONICET
url http://hdl.handle.net/11336/265111
identifier_str_mv Vereertbrugghen, Alexia; Pizzano, Manuela; Sabbione, Florencia; del Papa, Melina S.; Rodríguez, Giselle; et al.; Hyaluronate Protects From Benzalkonium Chloride-Induced Ocular Surface Toxicity; Association for Research in Vision and Ophthalmology; Translational Vision Science & Technology; 13; 10; 10-2024; 1-15
2164-2591
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://tvst.arvojournals.org/article.aspx?articleid=2802155
info:eu-repo/semantics/altIdentifier/doi/10.1167/tvst.13.10.31
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Association for Research in Vision and Ophthalmology
publisher.none.fl_str_mv Association for Research in Vision and Ophthalmology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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