Role of wnt/beta-catenin signaling in ovarian tumour growth and angiogenesis. A crosstalk with Notch system

Autores
Bocchicchio, Sebastian; Tesone, Marta; Irusta, Griselda
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Wnt/β-catenin and Notch are highly conserved pathways that regulate a diversity of cell processes, including proliferation, apoptosis and differentiation. We analyzed the role of both systems in ovarian cancer using specific inhibitors. For this purpose we performed three in vivo experiments. A human ovarian adenocarcinoma cell line (IGROV-1) was subcutaneously injected in 6-8 weeks-old female nude mice. Once the tumours were palpable, we injected the inhibitors: the first and second experiments were carried out using Wnt/β-catenin inhibitors (XAV939: 2.5, 5 mg/kg; ICG-001: 5 and10 mg/kg). The third experiment was a combination of ICG-001 (5mg/kg) and DAPT (5 mg/kg), a Notch inhibitor. Mice were injected every two days three times and they were euthanized 3 days after the last injection. Our results showed a significant decrease in tumour size when mice were treated either with XAV939 or with ICG-001. When compared with tumours from non-treated animals, both experiments showed a significant decrease in cell proliferation (KI67) and a decrease in the endothelial and periendothelial cell area stained with CD31 and α-Smooth-muscle-actin, respectively. When mice were treated with XAV939, a significant decrease in VEGF levels and Angiopoietin 1/2 was observed. Regarding the experiment with the combination of inhibitors, there was a significant decrease in tumour size and a decline in tumour cell proliferation (KI67). Both inhibitors administered simultaneously produced a decrease in cell proliferation at the same extent as individually administrated. In conclusion, we demonstrate a clear involvement of Wnt/β-catenin in ovarian tumour growth and angiognesis. We suggest an interaction of this pathway with Notch system.
Fil: Bocchicchio, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Tesone, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Irusta, Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
XX Jornadas anuales de la Sociedad Argentina de Biología. XVII Jornadas de la Sociedad Uruguaya de Biociencias
Buenos Aires
Argentina
Sociedad Argentina de Biología
Sociedad Uruguaya de Biología
Materia
WNT
OVARIAN TUMOR
GROWTH
ANGIOGENESIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/131526
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/131526
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role of wnt/beta-catenin signaling in ovarian tumour growth and angiogenesis. A crosstalk with Notch systemBocchicchio, SebastianTesone, MartaIrusta, GriseldaWNTOVARIAN TUMORGROWTHANGIOGENESIShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Wnt/β-catenin and Notch are highly conserved pathways that regulate a diversity of cell processes, including proliferation, apoptosis and differentiation. We analyzed the role of both systems in ovarian cancer using specific inhibitors. For this purpose we performed three in vivo experiments. A human ovarian adenocarcinoma cell line (IGROV-1) was subcutaneously injected in 6-8 weeks-old female nude mice. Once the tumours were palpable, we injected the inhibitors: the first and second experiments were carried out using Wnt/β-catenin inhibitors (XAV939: 2.5, 5 mg/kg; ICG-001: 5 and10 mg/kg). The third experiment was a combination of ICG-001 (5mg/kg) and DAPT (5 mg/kg), a Notch inhibitor. Mice were injected every two days three times and they were euthanized 3 days after the last injection. Our results showed a significant decrease in tumour size when mice were treated either with XAV939 or with ICG-001. When compared with tumours from non-treated animals, both experiments showed a significant decrease in cell proliferation (KI67) and a decrease in the endothelial and periendothelial cell area stained with CD31 and α-Smooth-muscle-actin, respectively. When mice were treated with XAV939, a significant decrease in VEGF levels and Angiopoietin 1/2 was observed. Regarding the experiment with the combination of inhibitors, there was a significant decrease in tumour size and a decline in tumour cell proliferation (KI67). Both inhibitors administered simultaneously produced a decrease in cell proliferation at the same extent as individually administrated. In conclusion, we demonstrate a clear involvement of Wnt/β-catenin in ovarian tumour growth and angiognesis. We suggest an interaction of this pathway with Notch system.Fil: Bocchicchio, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Tesone, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Irusta, Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaXX Jornadas anuales de la Sociedad Argentina de Biología. XVII Jornadas de la Sociedad Uruguaya de BiocienciasBuenos AiresArgentinaSociedad Argentina de BiologíaSociedad Uruguaya de BiologíaInstituto de Histología y Embriología2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectJornadaJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/131526Role of wnt/beta-catenin signaling in ovarian tumour growth and angiogenesis. A crosstalk with Notch system; XX Jornadas anuales de la Sociedad Argentina de Biología. XVII Jornadas de la Sociedad Uruguaya de Biociencias; Buenos Aires; Argentina; 2018; A58-A580327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v43nSuppl.3/33866Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:31Zoai:ri.conicet.gov.ar:11336/131526instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:31.628CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role of wnt/beta-catenin signaling in ovarian tumour growth and angiogenesis. A crosstalk with Notch system
title Role of wnt/beta-catenin signaling in ovarian tumour growth and angiogenesis. A crosstalk with Notch system
spellingShingle Role of wnt/beta-catenin signaling in ovarian tumour growth and angiogenesis. A crosstalk with Notch system
Bocchicchio, Sebastian
WNT
OVARIAN TUMOR
GROWTH
ANGIOGENESIS
title_short Role of wnt/beta-catenin signaling in ovarian tumour growth and angiogenesis. A crosstalk with Notch system
title_full Role of wnt/beta-catenin signaling in ovarian tumour growth and angiogenesis. A crosstalk with Notch system
title_fullStr Role of wnt/beta-catenin signaling in ovarian tumour growth and angiogenesis. A crosstalk with Notch system
title_full_unstemmed Role of wnt/beta-catenin signaling in ovarian tumour growth and angiogenesis. A crosstalk with Notch system
title_sort Role of wnt/beta-catenin signaling in ovarian tumour growth and angiogenesis. A crosstalk with Notch system
dc.creator.none.fl_str_mv Bocchicchio, Sebastian
Tesone, Marta
Irusta, Griselda
author Bocchicchio, Sebastian
author_facet Bocchicchio, Sebastian
Tesone, Marta
Irusta, Griselda
author_role author
author2 Tesone, Marta
Irusta, Griselda
author2_role author
author
dc.subject.none.fl_str_mv WNT
OVARIAN TUMOR
GROWTH
ANGIOGENESIS
topic WNT
OVARIAN TUMOR
GROWTH
ANGIOGENESIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Wnt/β-catenin and Notch are highly conserved pathways that regulate a diversity of cell processes, including proliferation, apoptosis and differentiation. We analyzed the role of both systems in ovarian cancer using specific inhibitors. For this purpose we performed three in vivo experiments. A human ovarian adenocarcinoma cell line (IGROV-1) was subcutaneously injected in 6-8 weeks-old female nude mice. Once the tumours were palpable, we injected the inhibitors: the first and second experiments were carried out using Wnt/β-catenin inhibitors (XAV939: 2.5, 5 mg/kg; ICG-001: 5 and10 mg/kg). The third experiment was a combination of ICG-001 (5mg/kg) and DAPT (5 mg/kg), a Notch inhibitor. Mice were injected every two days three times and they were euthanized 3 days after the last injection. Our results showed a significant decrease in tumour size when mice were treated either with XAV939 or with ICG-001. When compared with tumours from non-treated animals, both experiments showed a significant decrease in cell proliferation (KI67) and a decrease in the endothelial and periendothelial cell area stained with CD31 and α-Smooth-muscle-actin, respectively. When mice were treated with XAV939, a significant decrease in VEGF levels and Angiopoietin 1/2 was observed. Regarding the experiment with the combination of inhibitors, there was a significant decrease in tumour size and a decline in tumour cell proliferation (KI67). Both inhibitors administered simultaneously produced a decrease in cell proliferation at the same extent as individually administrated. In conclusion, we demonstrate a clear involvement of Wnt/β-catenin in ovarian tumour growth and angiognesis. We suggest an interaction of this pathway with Notch system.
Fil: Bocchicchio, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Tesone, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Irusta, Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
XX Jornadas anuales de la Sociedad Argentina de Biología. XVII Jornadas de la Sociedad Uruguaya de Biociencias
Buenos Aires
Argentina
Sociedad Argentina de Biología
Sociedad Uruguaya de Biología
description Wnt/β-catenin and Notch are highly conserved pathways that regulate a diversity of cell processes, including proliferation, apoptosis and differentiation. We analyzed the role of both systems in ovarian cancer using specific inhibitors. For this purpose we performed three in vivo experiments. A human ovarian adenocarcinoma cell line (IGROV-1) was subcutaneously injected in 6-8 weeks-old female nude mice. Once the tumours were palpable, we injected the inhibitors: the first and second experiments were carried out using Wnt/β-catenin inhibitors (XAV939: 2.5, 5 mg/kg; ICG-001: 5 and10 mg/kg). The third experiment was a combination of ICG-001 (5mg/kg) and DAPT (5 mg/kg), a Notch inhibitor. Mice were injected every two days three times and they were euthanized 3 days after the last injection. Our results showed a significant decrease in tumour size when mice were treated either with XAV939 or with ICG-001. When compared with tumours from non-treated animals, both experiments showed a significant decrease in cell proliferation (KI67) and a decrease in the endothelial and periendothelial cell area stained with CD31 and α-Smooth-muscle-actin, respectively. When mice were treated with XAV939, a significant decrease in VEGF levels and Angiopoietin 1/2 was observed. Regarding the experiment with the combination of inhibitors, there was a significant decrease in tumour size and a decline in tumour cell proliferation (KI67). Both inhibitors administered simultaneously produced a decrease in cell proliferation at the same extent as individually administrated. In conclusion, we demonstrate a clear involvement of Wnt/β-catenin in ovarian tumour growth and angiognesis. We suggest an interaction of this pathway with Notch system.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Jornada
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/131526
Role of wnt/beta-catenin signaling in ovarian tumour growth and angiogenesis. A crosstalk with Notch system; XX Jornadas anuales de la Sociedad Argentina de Biología. XVII Jornadas de la Sociedad Uruguaya de Biociencias; Buenos Aires; Argentina; 2018; A58-A58
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/131526
identifier_str_mv Role of wnt/beta-catenin signaling in ovarian tumour growth and angiogenesis. A crosstalk with Notch system; XX Jornadas anuales de la Sociedad Argentina de Biología. XVII Jornadas de la Sociedad Uruguaya de Biociencias; Buenos Aires; Argentina; 2018; A58-A58
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v43nSuppl.3/33866
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Instituto de Histología y Embriología
publisher.none.fl_str_mv Instituto de Histología y Embriología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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