Conservation of body sections and organs of the narrownose smooth-hound, Mustelus schmitti (Pisces, chondrichthyes), by silicone injection at room temperature to be used in compara...

Autores
Popp, Albertina Inés; Basso, Ana Paula; Lodovichi, Mariela Victoria; Sidorkewicj, Nora Silvia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Comparative Anatomy deals with the study of the ontogenetic and phylogenetic changes of the vertebrates, requiring complementing the theoretical aspects with the observation of structures in specimens belonging to different taxonomic groups. The aim of the present study was to test the injection of silicone at room temperature in organs and trunk sections of Mustelus schmitti as an alternative to the plastination technique. Samples consisted in brain, eyes, heart, proximal end of the ventral aorta, digestive tract, spleen, pancreas, kidneys, testis and cross body section at a pre-caudal level. Material was fixed with formalin (10-5 %), dehydrated with growing concentrations of isopropyl (30 % - 50 % - 70 % - 90 % - 100 % - 100 %), impregnated with diluted commercial silicone and cured at room temperature. The whole process took 66 days. The brain was the unique organ that could not undergo the complete procedure because it did not resist the injection of silicone. The other pieces resulted in materials that characterised by being off-colour, dry, semi-flexible, lightweight, odourless, and non-toxic. They showed no signs of fungal colonization or bacterial degradation after two years of being obtained. Shrinkage was observed, which ranged among 2-25 % for total length, and from 5-26 % for maximum width (mean values: 14 and 15 %, respectively), being testicle the organ that suffered greater shrinkage in both dimensions. The degree of contraction in length and width for each of the samples was generally similar (difference £ 3 %), indicating that not striking deformation occurred. Deformation was observed only for the trunk section, eye, stomach, pancreas and valvular intestine. The technique did not affect the morphology of the structures, allowing the correct visualization of all the basic features required to recognise them. We conclude that this simple and economic method is an adequate alternative to be implemented for the conservation of small-size materials with educational purposes in Comparative Anatomy courses.
Fil: Popp, Albertina Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Basso, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Lodovichi, Mariela Victoria. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sidorkewicj, Nora Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Materia
ACETIC SILICONE
COMPARATIVE ANATOMY
CONSERVATION TECHNIQUES
DISSECTION
EDUCATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/115920
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Conservation of body sections and organs of the narrownose smooth-hound, Mustelus schmitti (Pisces, chondrichthyes), by silicone injection at room temperature to be used in comparative anatomy learningConservación de secciones corporales y órganos de gatuzo, mustelus schmitti (Pisces, chondrichthyes), por inyección de silicona a temperatura ambiente para ser utilizados en el aprendizaje de anatomía comparadaPopp, Albertina InésBasso, Ana PaulaLodovichi, Mariela VictoriaSidorkewicj, Nora SilviaACETIC SILICONECOMPARATIVE ANATOMYCONSERVATION TECHNIQUESDISSECTIONEDUCATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Comparative Anatomy deals with the study of the ontogenetic and phylogenetic changes of the vertebrates, requiring complementing the theoretical aspects with the observation of structures in specimens belonging to different taxonomic groups. The aim of the present study was to test the injection of silicone at room temperature in organs and trunk sections of Mustelus schmitti as an alternative to the plastination technique. Samples consisted in brain, eyes, heart, proximal end of the ventral aorta, digestive tract, spleen, pancreas, kidneys, testis and cross body section at a pre-caudal level. Material was fixed with formalin (10-5 %), dehydrated with growing concentrations of isopropyl (30 % - 50 % - 70 % - 90 % - 100 % - 100 %), impregnated with diluted commercial silicone and cured at room temperature. The whole process took 66 days. The brain was the unique organ that could not undergo the complete procedure because it did not resist the injection of silicone. The other pieces resulted in materials that characterised by being off-colour, dry, semi-flexible, lightweight, odourless, and non-toxic. They showed no signs of fungal colonization or bacterial degradation after two years of being obtained. Shrinkage was observed, which ranged among 2-25 % for total length, and from 5-26 % for maximum width (mean values: 14 and 15 %, respectively), being testicle the organ that suffered greater shrinkage in both dimensions. The degree of contraction in length and width for each of the samples was generally similar (difference £ 3 %), indicating that not striking deformation occurred. Deformation was observed only for the trunk section, eye, stomach, pancreas and valvular intestine. The technique did not affect the morphology of the structures, allowing the correct visualization of all the basic features required to recognise them. We conclude that this simple and economic method is an adequate alternative to be implemented for the conservation of small-size materials with educational purposes in Comparative Anatomy courses.Fil: Popp, Albertina Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Basso, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Lodovichi, Mariela Victoria. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sidorkewicj, Nora Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaSociedad Chilena de Anatomía2018-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/115920Popp, Albertina Inés; Basso, Ana Paula; Lodovichi, Mariela Victoria; Sidorkewicj, Nora Silvia; Conservation of body sections and organs of the narrownose smooth-hound, Mustelus schmitti (Pisces, chondrichthyes), by silicone injection at room temperature to be used in comparative anatomy learning; Sociedad Chilena de Anatomía; International Journal of Morphology; 36; 2; 6-2018; 413-4180717-93670717-9502CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.intjmorphol.com/wp-content/uploads/2018/06/art_06_362.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.4067/S0717-95022018000200413info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:24:03Zoai:ri.conicet.gov.ar:11336/115920instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:24:03.951CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Conservation of body sections and organs of the narrownose smooth-hound, Mustelus schmitti (Pisces, chondrichthyes), by silicone injection at room temperature to be used in comparative anatomy learning
Conservación de secciones corporales y órganos de gatuzo, mustelus schmitti (Pisces, chondrichthyes), por inyección de silicona a temperatura ambiente para ser utilizados en el aprendizaje de anatomía comparada
title Conservation of body sections and organs of the narrownose smooth-hound, Mustelus schmitti (Pisces, chondrichthyes), by silicone injection at room temperature to be used in comparative anatomy learning
spellingShingle Conservation of body sections and organs of the narrownose smooth-hound, Mustelus schmitti (Pisces, chondrichthyes), by silicone injection at room temperature to be used in comparative anatomy learning
Popp, Albertina Inés
ACETIC SILICONE
COMPARATIVE ANATOMY
CONSERVATION TECHNIQUES
DISSECTION
EDUCATION
title_short Conservation of body sections and organs of the narrownose smooth-hound, Mustelus schmitti (Pisces, chondrichthyes), by silicone injection at room temperature to be used in comparative anatomy learning
title_full Conservation of body sections and organs of the narrownose smooth-hound, Mustelus schmitti (Pisces, chondrichthyes), by silicone injection at room temperature to be used in comparative anatomy learning
title_fullStr Conservation of body sections and organs of the narrownose smooth-hound, Mustelus schmitti (Pisces, chondrichthyes), by silicone injection at room temperature to be used in comparative anatomy learning
title_full_unstemmed Conservation of body sections and organs of the narrownose smooth-hound, Mustelus schmitti (Pisces, chondrichthyes), by silicone injection at room temperature to be used in comparative anatomy learning
title_sort Conservation of body sections and organs of the narrownose smooth-hound, Mustelus schmitti (Pisces, chondrichthyes), by silicone injection at room temperature to be used in comparative anatomy learning
dc.creator.none.fl_str_mv Popp, Albertina Inés
Basso, Ana Paula
Lodovichi, Mariela Victoria
Sidorkewicj, Nora Silvia
author Popp, Albertina Inés
author_facet Popp, Albertina Inés
Basso, Ana Paula
Lodovichi, Mariela Victoria
Sidorkewicj, Nora Silvia
author_role author
author2 Basso, Ana Paula
Lodovichi, Mariela Victoria
Sidorkewicj, Nora Silvia
author2_role author
author
author
dc.subject.none.fl_str_mv ACETIC SILICONE
COMPARATIVE ANATOMY
CONSERVATION TECHNIQUES
DISSECTION
EDUCATION
topic ACETIC SILICONE
COMPARATIVE ANATOMY
CONSERVATION TECHNIQUES
DISSECTION
EDUCATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Comparative Anatomy deals with the study of the ontogenetic and phylogenetic changes of the vertebrates, requiring complementing the theoretical aspects with the observation of structures in specimens belonging to different taxonomic groups. The aim of the present study was to test the injection of silicone at room temperature in organs and trunk sections of Mustelus schmitti as an alternative to the plastination technique. Samples consisted in brain, eyes, heart, proximal end of the ventral aorta, digestive tract, spleen, pancreas, kidneys, testis and cross body section at a pre-caudal level. Material was fixed with formalin (10-5 %), dehydrated with growing concentrations of isopropyl (30 % - 50 % - 70 % - 90 % - 100 % - 100 %), impregnated with diluted commercial silicone and cured at room temperature. The whole process took 66 days. The brain was the unique organ that could not undergo the complete procedure because it did not resist the injection of silicone. The other pieces resulted in materials that characterised by being off-colour, dry, semi-flexible, lightweight, odourless, and non-toxic. They showed no signs of fungal colonization or bacterial degradation after two years of being obtained. Shrinkage was observed, which ranged among 2-25 % for total length, and from 5-26 % for maximum width (mean values: 14 and 15 %, respectively), being testicle the organ that suffered greater shrinkage in both dimensions. The degree of contraction in length and width for each of the samples was generally similar (difference £ 3 %), indicating that not striking deformation occurred. Deformation was observed only for the trunk section, eye, stomach, pancreas and valvular intestine. The technique did not affect the morphology of the structures, allowing the correct visualization of all the basic features required to recognise them. We conclude that this simple and economic method is an adequate alternative to be implemented for the conservation of small-size materials with educational purposes in Comparative Anatomy courses.
Fil: Popp, Albertina Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Basso, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Lodovichi, Mariela Victoria. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sidorkewicj, Nora Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
description Comparative Anatomy deals with the study of the ontogenetic and phylogenetic changes of the vertebrates, requiring complementing the theoretical aspects with the observation of structures in specimens belonging to different taxonomic groups. The aim of the present study was to test the injection of silicone at room temperature in organs and trunk sections of Mustelus schmitti as an alternative to the plastination technique. Samples consisted in brain, eyes, heart, proximal end of the ventral aorta, digestive tract, spleen, pancreas, kidneys, testis and cross body section at a pre-caudal level. Material was fixed with formalin (10-5 %), dehydrated with growing concentrations of isopropyl (30 % - 50 % - 70 % - 90 % - 100 % - 100 %), impregnated with diluted commercial silicone and cured at room temperature. The whole process took 66 days. The brain was the unique organ that could not undergo the complete procedure because it did not resist the injection of silicone. The other pieces resulted in materials that characterised by being off-colour, dry, semi-flexible, lightweight, odourless, and non-toxic. They showed no signs of fungal colonization or bacterial degradation after two years of being obtained. Shrinkage was observed, which ranged among 2-25 % for total length, and from 5-26 % for maximum width (mean values: 14 and 15 %, respectively), being testicle the organ that suffered greater shrinkage in both dimensions. The degree of contraction in length and width for each of the samples was generally similar (difference £ 3 %), indicating that not striking deformation occurred. Deformation was observed only for the trunk section, eye, stomach, pancreas and valvular intestine. The technique did not affect the morphology of the structures, allowing the correct visualization of all the basic features required to recognise them. We conclude that this simple and economic method is an adequate alternative to be implemented for the conservation of small-size materials with educational purposes in Comparative Anatomy courses.
publishDate 2018
dc.date.none.fl_str_mv 2018-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/115920
Popp, Albertina Inés; Basso, Ana Paula; Lodovichi, Mariela Victoria; Sidorkewicj, Nora Silvia; Conservation of body sections and organs of the narrownose smooth-hound, Mustelus schmitti (Pisces, chondrichthyes), by silicone injection at room temperature to be used in comparative anatomy learning; Sociedad Chilena de Anatomía; International Journal of Morphology; 36; 2; 6-2018; 413-418
0717-9367
0717-9502
CONICET Digital
CONICET
url http://hdl.handle.net/11336/115920
identifier_str_mv Popp, Albertina Inés; Basso, Ana Paula; Lodovichi, Mariela Victoria; Sidorkewicj, Nora Silvia; Conservation of body sections and organs of the narrownose smooth-hound, Mustelus schmitti (Pisces, chondrichthyes), by silicone injection at room temperature to be used in comparative anatomy learning; Sociedad Chilena de Anatomía; International Journal of Morphology; 36; 2; 6-2018; 413-418
0717-9367
0717-9502
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.intjmorphol.com/wp-content/uploads/2018/06/art_06_362.pdf
info:eu-repo/semantics/altIdentifier/doi/10.4067/S0717-95022018000200413
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedad Chilena de Anatomía
publisher.none.fl_str_mv Sociedad Chilena de Anatomía
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.493442

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