Assessment of platelet activation in myeloproliferative disorders with complementary techniques

Autores
Bermejo, Emilse; Alberto, Maria Fabiana; Meschengieser, Susana S.; Lazzari, María Ángela
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bleeding and thrombosis in myeloproliferative disorders (MPD) are common events, sometimes both are present in the same patient during the course of the disease. Platelet activation in patients with MPD is often suggested. The present study analyses the presence of circulating activated platelets, using simultaneously flow cytometry and aggregometric studies in MPD. We studied 28 patients: 13 with polycythaemia vera, seven with essential thrombocythaemia, and eight chronic myeloid leukaemia. We performed functional tests, aggregation and adenosine triphosphate (ATP) release and flow cytometric assays (mepacrine staining and platelet activation markers CD62, CD63 and fibrinogen binding (B-FG)). Twenty-one MPD samples (75%) had reduced aggregation and ATP release. Acquired δ-SPD was detected in 11 of 28 MPD patients (39%), and we found no association between reduced mepacrine labelling and abnormal ATP release. High levels of activation markers were obtained: CD62 in 19 of 28 patients (68%), CD63 in 13 of 28 patients (46%) and B-FG in 19 of 28 patients (68%). The most prevalent abnormality was a reduced aggregation and ATP release. The lack of association between ATP release and mepacrine labelling suggests that other mechanisms, besides the deficit of intraplatelet ATP/adenosine diphosphate, might occur. High levels of activation markers were also observed. We conclude that both tests are complementary and necessary to understand the functional status of platelets in MPD.
Fil: Bermejo, Emilse. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Alberto, Maria Fabiana. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Meschengieser, Susana S.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Lazzari, María Ángela. Academia Nacional de Medicina de Buenos Aires; Argentina
Materia
Activated Platelets
Aggregation
Flow Cytometry
Myeloproliferative Disorders
Storage Pool Disease
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/71329
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Assessment of platelet activation in myeloproliferative disorders with complementary techniquesBermejo, EmilseAlberto, Maria FabianaMeschengieser, Susana S.Lazzari, María ÁngelaActivated PlateletsAggregationFlow CytometryMyeloproliferative DisordersStorage Pool Diseasehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Bleeding and thrombosis in myeloproliferative disorders (MPD) are common events, sometimes both are present in the same patient during the course of the disease. Platelet activation in patients with MPD is often suggested. The present study analyses the presence of circulating activated platelets, using simultaneously flow cytometry and aggregometric studies in MPD. We studied 28 patients: 13 with polycythaemia vera, seven with essential thrombocythaemia, and eight chronic myeloid leukaemia. We performed functional tests, aggregation and adenosine triphosphate (ATP) release and flow cytometric assays (mepacrine staining and platelet activation markers CD62, CD63 and fibrinogen binding (B-FG)). Twenty-one MPD samples (75%) had reduced aggregation and ATP release. Acquired δ-SPD was detected in 11 of 28 MPD patients (39%), and we found no association between reduced mepacrine labelling and abnormal ATP release. High levels of activation markers were obtained: CD62 in 19 of 28 patients (68%), CD63 in 13 of 28 patients (46%) and B-FG in 19 of 28 patients (68%). The most prevalent abnormality was a reduced aggregation and ATP release. The lack of association between ATP release and mepacrine labelling suggests that other mechanisms, besides the deficit of intraplatelet ATP/adenosine diphosphate, might occur. High levels of activation markers were also observed. We conclude that both tests are complementary and necessary to understand the functional status of platelets in MPD.Fil: Bermejo, Emilse. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Alberto, Maria Fabiana. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Meschengieser, Susana S.. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Lazzari, María Ángela. Academia Nacional de Medicina de Buenos Aires; ArgentinaLippincott Williams2004-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/71329Bermejo, Emilse; Alberto, Maria Fabiana; Meschengieser, Susana S.; Lazzari, María Ángela; Assessment of platelet activation in myeloproliferative disorders with complementary techniques; Lippincott Williams; Blood Coagulation & Fibrinolysis : An International Journal In Haemostasis And Thrombosis.; 15; 3; 4-2004; 235-2400957-52350957-52351473-5733CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/url/https://journals.lww.com/bloodcoagulation/pages/articleviewer.aspx?year=2004&issue=04000&article=00006&type=abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:25:03Zoai:ri.conicet.gov.ar:11336/71329instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:25:03.491CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Assessment of platelet activation in myeloproliferative disorders with complementary techniques
title Assessment of platelet activation in myeloproliferative disorders with complementary techniques
spellingShingle Assessment of platelet activation in myeloproliferative disorders with complementary techniques
Bermejo, Emilse
Activated Platelets
Aggregation
Flow Cytometry
Myeloproliferative Disorders
Storage Pool Disease
title_short Assessment of platelet activation in myeloproliferative disorders with complementary techniques
title_full Assessment of platelet activation in myeloproliferative disorders with complementary techniques
title_fullStr Assessment of platelet activation in myeloproliferative disorders with complementary techniques
title_full_unstemmed Assessment of platelet activation in myeloproliferative disorders with complementary techniques
title_sort Assessment of platelet activation in myeloproliferative disorders with complementary techniques
dc.creator.none.fl_str_mv Bermejo, Emilse
Alberto, Maria Fabiana
Meschengieser, Susana S.
Lazzari, María Ángela
author Bermejo, Emilse
author_facet Bermejo, Emilse
Alberto, Maria Fabiana
Meschengieser, Susana S.
Lazzari, María Ángela
author_role author
author2 Alberto, Maria Fabiana
Meschengieser, Susana S.
Lazzari, María Ángela
author2_role author
author
author
dc.subject.none.fl_str_mv Activated Platelets
Aggregation
Flow Cytometry
Myeloproliferative Disorders
Storage Pool Disease
topic Activated Platelets
Aggregation
Flow Cytometry
Myeloproliferative Disorders
Storage Pool Disease
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Bleeding and thrombosis in myeloproliferative disorders (MPD) are common events, sometimes both are present in the same patient during the course of the disease. Platelet activation in patients with MPD is often suggested. The present study analyses the presence of circulating activated platelets, using simultaneously flow cytometry and aggregometric studies in MPD. We studied 28 patients: 13 with polycythaemia vera, seven with essential thrombocythaemia, and eight chronic myeloid leukaemia. We performed functional tests, aggregation and adenosine triphosphate (ATP) release and flow cytometric assays (mepacrine staining and platelet activation markers CD62, CD63 and fibrinogen binding (B-FG)). Twenty-one MPD samples (75%) had reduced aggregation and ATP release. Acquired δ-SPD was detected in 11 of 28 MPD patients (39%), and we found no association between reduced mepacrine labelling and abnormal ATP release. High levels of activation markers were obtained: CD62 in 19 of 28 patients (68%), CD63 in 13 of 28 patients (46%) and B-FG in 19 of 28 patients (68%). The most prevalent abnormality was a reduced aggregation and ATP release. The lack of association between ATP release and mepacrine labelling suggests that other mechanisms, besides the deficit of intraplatelet ATP/adenosine diphosphate, might occur. High levels of activation markers were also observed. We conclude that both tests are complementary and necessary to understand the functional status of platelets in MPD.
Fil: Bermejo, Emilse. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Alberto, Maria Fabiana. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Meschengieser, Susana S.. Academia Nacional de Medicina de Buenos Aires; Argentina
Fil: Lazzari, María Ángela. Academia Nacional de Medicina de Buenos Aires; Argentina
description Bleeding and thrombosis in myeloproliferative disorders (MPD) are common events, sometimes both are present in the same patient during the course of the disease. Platelet activation in patients with MPD is often suggested. The present study analyses the presence of circulating activated platelets, using simultaneously flow cytometry and aggregometric studies in MPD. We studied 28 patients: 13 with polycythaemia vera, seven with essential thrombocythaemia, and eight chronic myeloid leukaemia. We performed functional tests, aggregation and adenosine triphosphate (ATP) release and flow cytometric assays (mepacrine staining and platelet activation markers CD62, CD63 and fibrinogen binding (B-FG)). Twenty-one MPD samples (75%) had reduced aggregation and ATP release. Acquired δ-SPD was detected in 11 of 28 MPD patients (39%), and we found no association between reduced mepacrine labelling and abnormal ATP release. High levels of activation markers were obtained: CD62 in 19 of 28 patients (68%), CD63 in 13 of 28 patients (46%) and B-FG in 19 of 28 patients (68%). The most prevalent abnormality was a reduced aggregation and ATP release. The lack of association between ATP release and mepacrine labelling suggests that other mechanisms, besides the deficit of intraplatelet ATP/adenosine diphosphate, might occur. High levels of activation markers were also observed. We conclude that both tests are complementary and necessary to understand the functional status of platelets in MPD.
publishDate 2004
dc.date.none.fl_str_mv 2004-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/71329
Bermejo, Emilse; Alberto, Maria Fabiana; Meschengieser, Susana S.; Lazzari, María Ángela; Assessment of platelet activation in myeloproliferative disorders with complementary techniques; Lippincott Williams; Blood Coagulation & Fibrinolysis : An International Journal In Haemostasis And Thrombosis.; 15; 3; 4-2004; 235-240
0957-5235
0957-5235
1473-5733
CONICET Digital
CONICET
url http://hdl.handle.net/11336/71329
identifier_str_mv Bermejo, Emilse; Alberto, Maria Fabiana; Meschengieser, Susana S.; Lazzari, María Ángela; Assessment of platelet activation in myeloproliferative disorders with complementary techniques; Lippincott Williams; Blood Coagulation & Fibrinolysis : An International Journal In Haemostasis And Thrombosis.; 15; 3; 4-2004; 235-240
0957-5235
1473-5733
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/url/https://journals.lww.com/bloodcoagulation/pages/articleviewer.aspx?year=2004&issue=04000&article=00006&type=abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Lippincott Williams
publisher.none.fl_str_mv Lippincott Williams
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614248618000384
score 13.070432

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