Heterorhabditis bacteriophora pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest Lobiopa insularis (Coleoptera: Nitidulidae)
- Autores
- Eliceche, Daiana Pamela; Belaich, Mariano Nicolas; Ghiringhelli, Pablo Daniel; Achinelly, Maria Fernanda
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The pampean region in the Buenos Aires province of Argentina is a major horticultural area. Biological pesticides to control pest insects available in the market are scarce in this country and nonexistent based on entomonematodes. In this paper, we characterized a native entomopathogenic nematode isolated from soil in the pampas, and evaluated its infectivity and reproductive capacity in the strawberry sap beetle Lobiopa insularis. Morphological and molecular-genetic analysis determined this isolate to be a nematode of Heterorhabditis bacteriophora, thereafter designated as the VEli strain. A 901-bp genomic-DNA fragment was isolated (GenBank Accesion No KJ575524). The new strain was pathogenic against L. insularis. In 200 ml containers with autoclaved clay soil (100 ml), at concentrations of 1,000; 5,000 and 10, 000 IJs, mortality in larvae ranged from 25 to 77 %. At a concentration of 10,000 IJs, infectivity was higher in larvae and pupae (77 ± 20% and 64 ± 25 %, respectively) than in adults (2.2 ± 1.1 %). The lethal concentration required to kill 50 and 90 % of the larvae (LC50 and LC90) over 10 days was 2,840 and 23,743 IJs. Differences were observed in the parasitism levels and emergence time of IJs between concentrations and stages, although not for production. Heterorhabditis bacteriophora strain VEli is the first entomopathogenic nematode found to infect and kill L. insularis, capable of completing its life cycle in all strawberry sap beetle stages. This availability would enable the establishment and subsequent dispersal of the strain in strawberry fields. Finally, this report constitutes the first presentation of data on the morphological, morphometric, and molecular-genetic characterization of a native entomopathogenic nematode strain from the Buenos Aires province, Argentina.
Fil: Eliceche, Daiana Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Belaich, Mariano Nicolas. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ghiringhelli, Pablo Daniel. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Achinelly, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Materia
-
BIOLOGICAL CONTROL
CHARACTERIZATION
ENTOMOPATHOGENIC NEMATODES
HETERORHABDITIDAE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/64124
Ver los metadatos del registro completo
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Heterorhabditis bacteriophora pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest Lobiopa insularis (Coleoptera: Nitidulidae)Heterorhabditis bacteriophora cepa-VEli (nematoda): Identificación y patogenia sobre lobiopa insularis (coleoptera: Nitidulidae) plaga de la fresaEliceche, Daiana PamelaBelaich, Mariano NicolasGhiringhelli, Pablo DanielAchinelly, Maria FernandaBIOLOGICAL CONTROLCHARACTERIZATIONENTOMOPATHOGENIC NEMATODESHETERORHABDITIDAEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The pampean region in the Buenos Aires province of Argentina is a major horticultural area. Biological pesticides to control pest insects available in the market are scarce in this country and nonexistent based on entomonematodes. In this paper, we characterized a native entomopathogenic nematode isolated from soil in the pampas, and evaluated its infectivity and reproductive capacity in the strawberry sap beetle Lobiopa insularis. Morphological and molecular-genetic analysis determined this isolate to be a nematode of Heterorhabditis bacteriophora, thereafter designated as the VEli strain. A 901-bp genomic-DNA fragment was isolated (GenBank Accesion No KJ575524). The new strain was pathogenic against L. insularis. In 200 ml containers with autoclaved clay soil (100 ml), at concentrations of 1,000; 5,000 and 10, 000 IJs, mortality in larvae ranged from 25 to 77 %. At a concentration of 10,000 IJs, infectivity was higher in larvae and pupae (77 ± 20% and 64 ± 25 %, respectively) than in adults (2.2 ± 1.1 %). The lethal concentration required to kill 50 and 90 % of the larvae (LC50 and LC90) over 10 days was 2,840 and 23,743 IJs. Differences were observed in the parasitism levels and emergence time of IJs between concentrations and stages, although not for production. Heterorhabditis bacteriophora strain VEli is the first entomopathogenic nematode found to infect and kill L. insularis, capable of completing its life cycle in all strawberry sap beetle stages. This availability would enable the establishment and subsequent dispersal of the strain in strawberry fields. Finally, this report constitutes the first presentation of data on the morphological, morphometric, and molecular-genetic characterization of a native entomopathogenic nematode strain from the Buenos Aires province, Argentina.Fil: Eliceche, Daiana Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Belaich, Mariano Nicolas. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ghiringhelli, Pablo Daniel. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Achinelly, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSociedad Colombiana de Entomologia2017-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/64124Eliceche, Daiana Pamela; Belaich, Mariano Nicolas; Ghiringhelli, Pablo Daniel; Achinelly, Maria Fernanda; Heterorhabditis bacteriophora pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest Lobiopa insularis (Coleoptera: Nitidulidae); Sociedad Colombiana de Entomologia; Revista Colombiana de Entomología; 43; 2; 7-2017; 223-2320120-0488CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://sociedadyeconomia.univalle.edu.co/index.php/SOCOLEN/article/view/5947info:eu-repo/semantics/altIdentifier/doi/10.25100/socolen.v43i2.5947info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:36:38Zoai:ri.conicet.gov.ar:11336/64124instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:36:38.571CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Heterorhabditis bacteriophora pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest Lobiopa insularis (Coleoptera: Nitidulidae) Heterorhabditis bacteriophora cepa-VEli (nematoda): Identificación y patogenia sobre lobiopa insularis (coleoptera: Nitidulidae) plaga de la fresa |
title |
Heterorhabditis bacteriophora pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest Lobiopa insularis (Coleoptera: Nitidulidae) |
spellingShingle |
Heterorhabditis bacteriophora pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest Lobiopa insularis (Coleoptera: Nitidulidae) Eliceche, Daiana Pamela BIOLOGICAL CONTROL CHARACTERIZATION ENTOMOPATHOGENIC NEMATODES HETERORHABDITIDAE |
title_short |
Heterorhabditis bacteriophora pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest Lobiopa insularis (Coleoptera: Nitidulidae) |
title_full |
Heterorhabditis bacteriophora pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest Lobiopa insularis (Coleoptera: Nitidulidae) |
title_fullStr |
Heterorhabditis bacteriophora pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest Lobiopa insularis (Coleoptera: Nitidulidae) |
title_full_unstemmed |
Heterorhabditis bacteriophora pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest Lobiopa insularis (Coleoptera: Nitidulidae) |
title_sort |
Heterorhabditis bacteriophora pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest Lobiopa insularis (Coleoptera: Nitidulidae) |
dc.creator.none.fl_str_mv |
Eliceche, Daiana Pamela Belaich, Mariano Nicolas Ghiringhelli, Pablo Daniel Achinelly, Maria Fernanda |
author |
Eliceche, Daiana Pamela |
author_facet |
Eliceche, Daiana Pamela Belaich, Mariano Nicolas Ghiringhelli, Pablo Daniel Achinelly, Maria Fernanda |
author_role |
author |
author2 |
Belaich, Mariano Nicolas Ghiringhelli, Pablo Daniel Achinelly, Maria Fernanda |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
BIOLOGICAL CONTROL CHARACTERIZATION ENTOMOPATHOGENIC NEMATODES HETERORHABDITIDAE |
topic |
BIOLOGICAL CONTROL CHARACTERIZATION ENTOMOPATHOGENIC NEMATODES HETERORHABDITIDAE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The pampean region in the Buenos Aires province of Argentina is a major horticultural area. Biological pesticides to control pest insects available in the market are scarce in this country and nonexistent based on entomonematodes. In this paper, we characterized a native entomopathogenic nematode isolated from soil in the pampas, and evaluated its infectivity and reproductive capacity in the strawberry sap beetle Lobiopa insularis. Morphological and molecular-genetic analysis determined this isolate to be a nematode of Heterorhabditis bacteriophora, thereafter designated as the VEli strain. A 901-bp genomic-DNA fragment was isolated (GenBank Accesion No KJ575524). The new strain was pathogenic against L. insularis. In 200 ml containers with autoclaved clay soil (100 ml), at concentrations of 1,000; 5,000 and 10, 000 IJs, mortality in larvae ranged from 25 to 77 %. At a concentration of 10,000 IJs, infectivity was higher in larvae and pupae (77 ± 20% and 64 ± 25 %, respectively) than in adults (2.2 ± 1.1 %). The lethal concentration required to kill 50 and 90 % of the larvae (LC50 and LC90) over 10 days was 2,840 and 23,743 IJs. Differences were observed in the parasitism levels and emergence time of IJs between concentrations and stages, although not for production. Heterorhabditis bacteriophora strain VEli is the first entomopathogenic nematode found to infect and kill L. insularis, capable of completing its life cycle in all strawberry sap beetle stages. This availability would enable the establishment and subsequent dispersal of the strain in strawberry fields. Finally, this report constitutes the first presentation of data on the morphological, morphometric, and molecular-genetic characterization of a native entomopathogenic nematode strain from the Buenos Aires province, Argentina. Fil: Eliceche, Daiana Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina Fil: Belaich, Mariano Nicolas. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ghiringhelli, Pablo Daniel. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Achinelly, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
The pampean region in the Buenos Aires province of Argentina is a major horticultural area. Biological pesticides to control pest insects available in the market are scarce in this country and nonexistent based on entomonematodes. In this paper, we characterized a native entomopathogenic nematode isolated from soil in the pampas, and evaluated its infectivity and reproductive capacity in the strawberry sap beetle Lobiopa insularis. Morphological and molecular-genetic analysis determined this isolate to be a nematode of Heterorhabditis bacteriophora, thereafter designated as the VEli strain. A 901-bp genomic-DNA fragment was isolated (GenBank Accesion No KJ575524). The new strain was pathogenic against L. insularis. In 200 ml containers with autoclaved clay soil (100 ml), at concentrations of 1,000; 5,000 and 10, 000 IJs, mortality in larvae ranged from 25 to 77 %. At a concentration of 10,000 IJs, infectivity was higher in larvae and pupae (77 ± 20% and 64 ± 25 %, respectively) than in adults (2.2 ± 1.1 %). The lethal concentration required to kill 50 and 90 % of the larvae (LC50 and LC90) over 10 days was 2,840 and 23,743 IJs. Differences were observed in the parasitism levels and emergence time of IJs between concentrations and stages, although not for production. Heterorhabditis bacteriophora strain VEli is the first entomopathogenic nematode found to infect and kill L. insularis, capable of completing its life cycle in all strawberry sap beetle stages. This availability would enable the establishment and subsequent dispersal of the strain in strawberry fields. Finally, this report constitutes the first presentation of data on the morphological, morphometric, and molecular-genetic characterization of a native entomopathogenic nematode strain from the Buenos Aires province, Argentina. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/64124 Eliceche, Daiana Pamela; Belaich, Mariano Nicolas; Ghiringhelli, Pablo Daniel; Achinelly, Maria Fernanda; Heterorhabditis bacteriophora pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest Lobiopa insularis (Coleoptera: Nitidulidae); Sociedad Colombiana de Entomologia; Revista Colombiana de Entomología; 43; 2; 7-2017; 223-232 0120-0488 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/64124 |
identifier_str_mv |
Eliceche, Daiana Pamela; Belaich, Mariano Nicolas; Ghiringhelli, Pablo Daniel; Achinelly, Maria Fernanda; Heterorhabditis bacteriophora pampean-strain VEli (Nematoda): identification and pathogenicity against the strawberry pest Lobiopa insularis (Coleoptera: Nitidulidae); Sociedad Colombiana de Entomologia; Revista Colombiana de Entomología; 43; 2; 7-2017; 223-232 0120-0488 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://sociedadyeconomia.univalle.edu.co/index.php/SOCOLEN/article/view/5947 info:eu-repo/semantics/altIdentifier/doi/10.25100/socolen.v43i2.5947 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Sociedad Colombiana de Entomologia |
publisher.none.fl_str_mv |
Sociedad Colombiana de Entomologia |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614386975506432 |
score |
13.070432 |