C-banding, fluorescent staining and NOR location in holokinetic chromosomes of bugs of the Neotropical genus Antiteuchus (Heteroptera: Pentatomidae: Discocephalinae)

Autores
Lanzone, Cecilia; de Souza, Maria José
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Different cytogenetic techniques including C-banding, base-specific fluorochromes and silver nitrate staining were used to compare the karyotypes of three species of bugs, representatives of the Neotropical genus Antiteuchus, namely A. mixtus, A. sepulcralis and A. macraspis. The species have holokinetic chromosomes which is typical of the Hemiptera, and their diploid chromosome number is 2n = 14 and they have an XX/XY (female/male) sex chromosome system. C-banding revealed telomeric blocks of constitutive heterochromatin in most chromosomes of these three species. In each species, CMA;-positive blocks were observed in the telomeric heterochromatin region of chromosome pair 1. This chromosome pair carried the nucleolar organizer region (NOR), which silver nitrate staining revealed is located in the telomeric heterochromatin. In contrast to the result obtained with CMA3, entire chromosome complement stained homogenously with DAPI, except for the telomeric region of pair 1, which remained unstained.
Fil: Lanzone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina. Universidade Federal de Pernambuco; Brasil
Fil: de Souza, Maria José. Universidade Federal de Pernambuco; Brasil
Materia
ANTITEUCHUS
C-BANDING
FLUOROCHROMES
HETEROPTERA
HOLOKINETIC CHROMOSOMES
NORS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/99741
Acceder
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network_name_str CONICET Digital (CONICET)
spelling C-banding, fluorescent staining and NOR location in holokinetic chromosomes of bugs of the Neotropical genus Antiteuchus (Heteroptera: Pentatomidae: Discocephalinae)Lanzone, Ceciliade Souza, Maria JoséANTITEUCHUSC-BANDINGFLUOROCHROMESHETEROPTERAHOLOKINETIC CHROMOSOMESNORShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Different cytogenetic techniques including C-banding, base-specific fluorochromes and silver nitrate staining were used to compare the karyotypes of three species of bugs, representatives of the Neotropical genus Antiteuchus, namely A. mixtus, A. sepulcralis and A. macraspis. The species have holokinetic chromosomes which is typical of the Hemiptera, and their diploid chromosome number is 2n = 14 and they have an XX/XY (female/male) sex chromosome system. C-banding revealed telomeric blocks of constitutive heterochromatin in most chromosomes of these three species. In each species, CMA;-positive blocks were observed in the telomeric heterochromatin region of chromosome pair 1. This chromosome pair carried the nucleolar organizer region (NOR), which silver nitrate staining revealed is located in the telomeric heterochromatin. In contrast to the result obtained with CMA3, entire chromosome complement stained homogenously with DAPI, except for the telomeric region of pair 1, which remained unstained.Fil: Lanzone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina. Universidade Federal de Pernambuco; BrasilFil: de Souza, Maria José. Universidade Federal de Pernambuco; BrasilCzech Academy of Sciences2006-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/99741Lanzone, Cecilia; de Souza, Maria José; C-banding, fluorescent staining and NOR location in holokinetic chromosomes of bugs of the Neotropical genus Antiteuchus (Heteroptera: Pentatomidae: Discocephalinae); Czech Academy of Sciences; European Journal of Entomology (Ceske Budejovice); 103; 1; 12-2006; 239-2431210-5759CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.eje.cz/artkey/eje-200601-0026_c-banding_fluorescent_staining_and_nor_location_in_holokinetic_chromosomes_of_bugs_of_the_neotropical_genus_an.phpinfo:eu-repo/semantics/altIdentifier/doi/10.14411/eje.2006.026info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:09Zoai:ri.conicet.gov.ar:11336/99741instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:09.607CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv C-banding, fluorescent staining and NOR location in holokinetic chromosomes of bugs of the Neotropical genus Antiteuchus (Heteroptera: Pentatomidae: Discocephalinae)
title C-banding, fluorescent staining and NOR location in holokinetic chromosomes of bugs of the Neotropical genus Antiteuchus (Heteroptera: Pentatomidae: Discocephalinae)
spellingShingle C-banding, fluorescent staining and NOR location in holokinetic chromosomes of bugs of the Neotropical genus Antiteuchus (Heteroptera: Pentatomidae: Discocephalinae)
Lanzone, Cecilia
ANTITEUCHUS
C-BANDING
FLUOROCHROMES
HETEROPTERA
HOLOKINETIC CHROMOSOMES
NORS
title_short C-banding, fluorescent staining and NOR location in holokinetic chromosomes of bugs of the Neotropical genus Antiteuchus (Heteroptera: Pentatomidae: Discocephalinae)
title_full C-banding, fluorescent staining and NOR location in holokinetic chromosomes of bugs of the Neotropical genus Antiteuchus (Heteroptera: Pentatomidae: Discocephalinae)
title_fullStr C-banding, fluorescent staining and NOR location in holokinetic chromosomes of bugs of the Neotropical genus Antiteuchus (Heteroptera: Pentatomidae: Discocephalinae)
title_full_unstemmed C-banding, fluorescent staining and NOR location in holokinetic chromosomes of bugs of the Neotropical genus Antiteuchus (Heteroptera: Pentatomidae: Discocephalinae)
title_sort C-banding, fluorescent staining and NOR location in holokinetic chromosomes of bugs of the Neotropical genus Antiteuchus (Heteroptera: Pentatomidae: Discocephalinae)
dc.creator.none.fl_str_mv Lanzone, Cecilia
de Souza, Maria José
author Lanzone, Cecilia
author_facet Lanzone, Cecilia
de Souza, Maria José
author_role author
author2 de Souza, Maria José
author2_role author
dc.subject.none.fl_str_mv ANTITEUCHUS
C-BANDING
FLUOROCHROMES
HETEROPTERA
HOLOKINETIC CHROMOSOMES
NORS
topic ANTITEUCHUS
C-BANDING
FLUOROCHROMES
HETEROPTERA
HOLOKINETIC CHROMOSOMES
NORS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Different cytogenetic techniques including C-banding, base-specific fluorochromes and silver nitrate staining were used to compare the karyotypes of three species of bugs, representatives of the Neotropical genus Antiteuchus, namely A. mixtus, A. sepulcralis and A. macraspis. The species have holokinetic chromosomes which is typical of the Hemiptera, and their diploid chromosome number is 2n = 14 and they have an XX/XY (female/male) sex chromosome system. C-banding revealed telomeric blocks of constitutive heterochromatin in most chromosomes of these three species. In each species, CMA;-positive blocks were observed in the telomeric heterochromatin region of chromosome pair 1. This chromosome pair carried the nucleolar organizer region (NOR), which silver nitrate staining revealed is located in the telomeric heterochromatin. In contrast to the result obtained with CMA3, entire chromosome complement stained homogenously with DAPI, except for the telomeric region of pair 1, which remained unstained.
Fil: Lanzone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina. Universidade Federal de Pernambuco; Brasil
Fil: de Souza, Maria José. Universidade Federal de Pernambuco; Brasil
description Different cytogenetic techniques including C-banding, base-specific fluorochromes and silver nitrate staining were used to compare the karyotypes of three species of bugs, representatives of the Neotropical genus Antiteuchus, namely A. mixtus, A. sepulcralis and A. macraspis. The species have holokinetic chromosomes which is typical of the Hemiptera, and their diploid chromosome number is 2n = 14 and they have an XX/XY (female/male) sex chromosome system. C-banding revealed telomeric blocks of constitutive heterochromatin in most chromosomes of these three species. In each species, CMA;-positive blocks were observed in the telomeric heterochromatin region of chromosome pair 1. This chromosome pair carried the nucleolar organizer region (NOR), which silver nitrate staining revealed is located in the telomeric heterochromatin. In contrast to the result obtained with CMA3, entire chromosome complement stained homogenously with DAPI, except for the telomeric region of pair 1, which remained unstained.
publishDate 2006
dc.date.none.fl_str_mv 2006-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/99741
Lanzone, Cecilia; de Souza, Maria José; C-banding, fluorescent staining and NOR location in holokinetic chromosomes of bugs of the Neotropical genus Antiteuchus (Heteroptera: Pentatomidae: Discocephalinae); Czech Academy of Sciences; European Journal of Entomology (Ceske Budejovice); 103; 1; 12-2006; 239-243
1210-5759
CONICET Digital
CONICET
url http://hdl.handle.net/11336/99741
identifier_str_mv Lanzone, Cecilia; de Souza, Maria José; C-banding, fluorescent staining and NOR location in holokinetic chromosomes of bugs of the Neotropical genus Antiteuchus (Heteroptera: Pentatomidae: Discocephalinae); Czech Academy of Sciences; European Journal of Entomology (Ceske Budejovice); 103; 1; 12-2006; 239-243
1210-5759
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.eje.cz/artkey/eje-200601-0026_c-banding_fluorescent_staining_and_nor_location_in_holokinetic_chromosomes_of_bugs_of_the_neotropical_genus_an.php
info:eu-repo/semantics/altIdentifier/doi/10.14411/eje.2006.026
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Czech Academy of Sciences
publisher.none.fl_str_mv Czech Academy of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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