New era in plant alternative splicing analysis enabled by advances in high-throughput sequencing (HTS) technologies

• Autores: Bedre, Renesh; Irigoyen, Sonia; Petrillo, Ezequiel; Mandadi, Kranthi
• Año de publicación: 2019
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: ALTERNATIVE SPLICING; BIOINFORMATICS; HIGH-THROUGHPUT SEQUENCING; NON-SENSE-MEDIATED DECAY; PCR; RNA-SEQ
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Alternative splicing (AS) is a crucial posttranscriptional mechanism of gene expression which promotes transcriptome and proteome diversity. At the molecular level, splicing and AS involves recognition and elimination of intronic regions of a precursor messenger RNA (pre-mRNA) and joining of exonic regions to generate the mature mRNA. AS generates more than one mRNA transcript (transcripts) differing in coding and/or untranslated regions (UTRs). AS can be classified into four major types including the exon skipping (ES), intron retention (IR), alternative donor (AD), and alternative acceptor (AA), of which IR is the most prevalent event in plants (Mandadi and Scholthof, 2015). In addition to these AS types, a subfamily of IR called exitrons, which has dual features of introns and protein-coding exons were first reported in Arabidopsis thaliana (Arabidopsis) and later also found in humans (Marquez et al., 2015). These spliced transcripts influence multiple biological processes such as growth, development and response to biotic and abiotic stresses in plants (Filichkin et al., 2015; Mandadi and Scholthof, 2015; Wang et al., 2018a).
Fil: Bedre, Renesh. Texas A&m University Commerce; Estados Unidos
Fil: Irigoyen, Sonia. Texas A&m University Commerce; Estados Unidos
Fil: Petrillo, Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Mandadi, Kranthi. Texas A&m University Commerce; Estados Unidos