Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus Determinación del voltaje y el tamaño del an...

Autores
Viana da Costa, Tiago; Yoshii Oshiro, Lidia Miyako; Lopez, Laura Susana; Melo, Emanuela Paula; Antunes de Mattos, Luciana; Bambozzi Fernandes, Andrea
Año de publicación
2016
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
So far Macrobrachium acanthurus reproduction studies are poorly advanced being important if there is the possibility of cultivation. The aim of this study was to identify the most suitable voltage for the extraction of spermatophores and the smaller size at which these prawns produce them, as a prior step to develop in vitro fertilization techniques. Voltages of 4.5 and 6.0 were used, performing sperm count based on survival of sperm smears, colored with eosin-nigrosine. In the study of size, prawns were separated into class intervals and analyzed for production of spermatophore and sperm survival. The best voltage for the extraction of seminal material due to lower mortality was 6.0 volts and from 5 g of body weight, and 18 mm of length prawns could be used for the extraction of spermatophores.
Fil: Viana da Costa, Tiago. Universidade Federal Do Amazonas; Brasil
Fil: Yoshii Oshiro, Lidia Miyako. Universidade Federal do Rio de Janeiro; Brasil
Fil: Lopez, Laura Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Melo, Emanuela Paula. Universidade Federal do Rio de Janeiro; Brasil
Fil: Antunes de Mattos, Luciana. Universidade Federal do Rio de Janeiro; Brasil
Fil: Bambozzi Fernandes, Andrea. Universidade Federal do Rio de Janeiro; Brasil
Materia
CARIDEA
MACROBRACHIUM ACANTHURUS
REPRODUCTION
SPERM
SPERM SURVIVAL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/60805
Acceder
id CONICETDig_afee3598ba563c9a5dcb0753785fc9d0
oai_identifier_str oai:ri.conicet.gov.ar:11336/60805
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus Determinación del voltaje y el tamaño del animal óptimos para la extracción de espermatóforos en el camarón de agua dulce Macrobrachium acanthurusViana da Costa, TiagoYoshii Oshiro, Lidia MiyakoLopez, Laura SusanaMelo, Emanuela PaulaAntunes de Mattos, LucianaBambozzi Fernandes, AndreaCARIDEAMACROBRACHIUM ACANTHURUSREPRODUCTIONSPERMSPERM SURVIVALhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1So far Macrobrachium acanthurus reproduction studies are poorly advanced being important if there is the possibility of cultivation. The aim of this study was to identify the most suitable voltage for the extraction of spermatophores and the smaller size at which these prawns produce them, as a prior step to develop in vitro fertilization techniques. Voltages of 4.5 and 6.0 were used, performing sperm count based on survival of sperm smears, colored with eosin-nigrosine. In the study of size, prawns were separated into class intervals and analyzed for production of spermatophore and sperm survival. The best voltage for the extraction of seminal material due to lower mortality was 6.0 volts and from 5 g of body weight, and 18 mm of length prawns could be used for the extraction of spermatophores.Fil: Viana da Costa, Tiago. Universidade Federal Do Amazonas; BrasilFil: Yoshii Oshiro, Lidia Miyako. Universidade Federal do Rio de Janeiro; BrasilFil: Lopez, Laura Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; ArgentinaFil: Melo, Emanuela Paula. Universidade Federal do Rio de Janeiro; BrasilFil: Antunes de Mattos, Luciana. Universidade Federal do Rio de Janeiro; BrasilFil: Bambozzi Fernandes, Andrea. Universidade Federal do Rio de Janeiro; BrasilPontificia Universidad Catolica de Valparaíso2016-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/60805Viana da Costa, Tiago; Yoshii Oshiro, Lidia Miyako; Lopez, Laura Susana; Melo, Emanuela Paula; Antunes de Mattos, Luciana; et al.; Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus Determinación del voltaje y el tamaño del animal óptimos para la extracción de espermatóforos en el camarón de agua dulce Macrobrachium acanthurus; Pontificia Universidad Catolica de Valparaíso; Latin American Journal of Aquatic Research; 44; 2; 5-2016; 422-4280718-560XCONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/http://www.lajar.cl/pdf/imar/v44n2/Art%C3%ADculo_44_2_24.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.3856/vol44-issue2-fulltext-24info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:09Zoai:ri.conicet.gov.ar:11336/60805instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:09.59CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus Determinación del voltaje y el tamaño del animal óptimos para la extracción de espermatóforos en el camarón de agua dulce Macrobrachium acanthurus
title Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus Determinación del voltaje y el tamaño del animal óptimos para la extracción de espermatóforos en el camarón de agua dulce Macrobrachium acanthurus
spellingShingle Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus Determinación del voltaje y el tamaño del animal óptimos para la extracción de espermatóforos en el camarón de agua dulce Macrobrachium acanthurus
Viana da Costa, Tiago
CARIDEA
MACROBRACHIUM ACANTHURUS
REPRODUCTION
SPERM
SPERM SURVIVAL
title_short Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus Determinación del voltaje y el tamaño del animal óptimos para la extracción de espermatóforos en el camarón de agua dulce Macrobrachium acanthurus
title_full Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus Determinación del voltaje y el tamaño del animal óptimos para la extracción de espermatóforos en el camarón de agua dulce Macrobrachium acanthurus
title_fullStr Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus Determinación del voltaje y el tamaño del animal óptimos para la extracción de espermatóforos en el camarón de agua dulce Macrobrachium acanthurus
title_full_unstemmed Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus Determinación del voltaje y el tamaño del animal óptimos para la extracción de espermatóforos en el camarón de agua dulce Macrobrachium acanthurus
title_sort Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus Determinación del voltaje y el tamaño del animal óptimos para la extracción de espermatóforos en el camarón de agua dulce Macrobrachium acanthurus
dc.creator.none.fl_str_mv Viana da Costa, Tiago
Yoshii Oshiro, Lidia Miyako
Lopez, Laura Susana
Melo, Emanuela Paula
Antunes de Mattos, Luciana
Bambozzi Fernandes, Andrea
author Viana da Costa, Tiago
author_facet Viana da Costa, Tiago
Yoshii Oshiro, Lidia Miyako
Lopez, Laura Susana
Melo, Emanuela Paula
Antunes de Mattos, Luciana
Bambozzi Fernandes, Andrea
author_role author
author2 Yoshii Oshiro, Lidia Miyako
Lopez, Laura Susana
Melo, Emanuela Paula
Antunes de Mattos, Luciana
Bambozzi Fernandes, Andrea
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CARIDEA
MACROBRACHIUM ACANTHURUS
REPRODUCTION
SPERM
SPERM SURVIVAL
topic CARIDEA
MACROBRACHIUM ACANTHURUS
REPRODUCTION
SPERM
SPERM SURVIVAL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv So far Macrobrachium acanthurus reproduction studies are poorly advanced being important if there is the possibility of cultivation. The aim of this study was to identify the most suitable voltage for the extraction of spermatophores and the smaller size at which these prawns produce them, as a prior step to develop in vitro fertilization techniques. Voltages of 4.5 and 6.0 were used, performing sperm count based on survival of sperm smears, colored with eosin-nigrosine. In the study of size, prawns were separated into class intervals and analyzed for production of spermatophore and sperm survival. The best voltage for the extraction of seminal material due to lower mortality was 6.0 volts and from 5 g of body weight, and 18 mm of length prawns could be used for the extraction of spermatophores.
Fil: Viana da Costa, Tiago. Universidade Federal Do Amazonas; Brasil
Fil: Yoshii Oshiro, Lidia Miyako. Universidade Federal do Rio de Janeiro; Brasil
Fil: Lopez, Laura Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
Fil: Melo, Emanuela Paula. Universidade Federal do Rio de Janeiro; Brasil
Fil: Antunes de Mattos, Luciana. Universidade Federal do Rio de Janeiro; Brasil
Fil: Bambozzi Fernandes, Andrea. Universidade Federal do Rio de Janeiro; Brasil
description So far Macrobrachium acanthurus reproduction studies are poorly advanced being important if there is the possibility of cultivation. The aim of this study was to identify the most suitable voltage for the extraction of spermatophores and the smaller size at which these prawns produce them, as a prior step to develop in vitro fertilization techniques. Voltages of 4.5 and 6.0 were used, performing sperm count based on survival of sperm smears, colored with eosin-nigrosine. In the study of size, prawns were separated into class intervals and analyzed for production of spermatophore and sperm survival. The best voltage for the extraction of seminal material due to lower mortality was 6.0 volts and from 5 g of body weight, and 18 mm of length prawns could be used for the extraction of spermatophores.
publishDate 2016
dc.date.none.fl_str_mv 2016-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/60805
Viana da Costa, Tiago; Yoshii Oshiro, Lidia Miyako; Lopez, Laura Susana; Melo, Emanuela Paula; Antunes de Mattos, Luciana; et al.; Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus Determinación del voltaje y el tamaño del animal óptimos para la extracción de espermatóforos en el camarón de agua dulce Macrobrachium acanthurus; Pontificia Universidad Catolica de Valparaíso; Latin American Journal of Aquatic Research; 44; 2; 5-2016; 422-428
0718-560X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/60805
identifier_str_mv Viana da Costa, Tiago; Yoshii Oshiro, Lidia Miyako; Lopez, Laura Susana; Melo, Emanuela Paula; Antunes de Mattos, Luciana; et al.; Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus Determinación del voltaje y el tamaño del animal óptimos para la extracción de espermatóforos en el camarón de agua dulce Macrobrachium acanthurus; Pontificia Universidad Catolica de Valparaíso; Latin American Journal of Aquatic Research; 44; 2; 5-2016; 422-428
0718-560X
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.lajar.cl/pdf/imar/v44n2/Art%C3%ADculo_44_2_24.pdf
info:eu-repo/semantics/altIdentifier/doi/10.3856/vol44-issue2-fulltext-24
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pontificia Universidad Catolica de Valparaíso
publisher.none.fl_str_mv Pontificia Universidad Catolica de Valparaíso
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269076894777344
score 13.13397

Publicaciones similares