Tumor cell lines resistant to ALA-mediated photodynamic therapy and possible tools to target surviving cells
- Autores
- Casas, Adriana Gabriela; Perotti, Christian; Ortel, Bernhard; Di Venosa, Gabriela Mariana; Saccoliti, María; Batlle, Alcira Maria del C.; Hasan, Tayyaba
- Año de publicación
- 2006
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We isolated and characterized cell lines resistant to aminolevulinic acid (ALA)-mediated photodynamic therapy (PDT) derived from a murine adenocarcinoma and studied cross resistance with other injuries. The most resistant clones were numbers 4 and 8, which exhibited 6.7- and 4.2-fold increase in resistance respectively. Several characteristics were altered in these clones. A 2-fold increase in cell volume, higher cell spreading, and a more fibroblastic, dendritic pattern, were the morphology features that led us to think they could have different adhesive, invasive or metastatic phenotypes. The amount of porphyrins synthesized per cell in the resistant clones was similar to the parental line but, when it was expressed per mg protein, there was a 2-fold decrease, with a higher proportion of hydrophilic porphyrins. These cells were not cross-resistant to photosensitization with Benzoporphyrin derivative and Merocyanine 540, but exhibited a slight resistance to exogenous protoporphyrin IX treatment. Both clones displayed higher protein content and increased number of mitochondria, together with a higher oxygen consumption. The distinctive features found in the resistant lines led as to think how to exploit the changes induced by PDT treatment to target surviving cells. Those hypoxic cells can be also a preferential target of bioreductive drugs and hypoxia-directed gene therapy, and would be sensitive to treatment with other photosensitizers.
Fil: Casas, Adriana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Perotti, Christian. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Ortel, Bernhard. Harvard Medical School; Estados Unidos
Fil: Di Venosa, Gabriela Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Saccoliti, María. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Carlos Durand; Argentina
Fil: Batlle, Alcira Maria del C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina
Fil: Hasan, Tayyaba. Harvard Medical School; Estados Unidos - Materia
-
AMINOLEVULINIC ACID
CROSS-RESISTANCE
HYPOXIA
PHOTODYNAMIC THERAPY
RESISTANCE - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/152593
Ver los metadatos del registro completo
id |
CONICETDig_afd78e101e965f954199702a0b8e4a11 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/152593 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Tumor cell lines resistant to ALA-mediated photodynamic therapy and possible tools to target surviving cellsCasas, Adriana GabrielaPerotti, ChristianOrtel, BernhardDi Venosa, Gabriela MarianaSaccoliti, MaríaBatlle, Alcira Maria del C.Hasan, TayyabaAMINOLEVULINIC ACIDCROSS-RESISTANCEHYPOXIAPHOTODYNAMIC THERAPYRESISTANCEhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3We isolated and characterized cell lines resistant to aminolevulinic acid (ALA)-mediated photodynamic therapy (PDT) derived from a murine adenocarcinoma and studied cross resistance with other injuries. The most resistant clones were numbers 4 and 8, which exhibited 6.7- and 4.2-fold increase in resistance respectively. Several characteristics were altered in these clones. A 2-fold increase in cell volume, higher cell spreading, and a more fibroblastic, dendritic pattern, were the morphology features that led us to think they could have different adhesive, invasive or metastatic phenotypes. The amount of porphyrins synthesized per cell in the resistant clones was similar to the parental line but, when it was expressed per mg protein, there was a 2-fold decrease, with a higher proportion of hydrophilic porphyrins. These cells were not cross-resistant to photosensitization with Benzoporphyrin derivative and Merocyanine 540, but exhibited a slight resistance to exogenous protoporphyrin IX treatment. Both clones displayed higher protein content and increased number of mitochondria, together with a higher oxygen consumption. The distinctive features found in the resistant lines led as to think how to exploit the changes induced by PDT treatment to target surviving cells. Those hypoxic cells can be also a preferential target of bioreductive drugs and hypoxia-directed gene therapy, and would be sensitive to treatment with other photosensitizers.Fil: Casas, Adriana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; ArgentinaFil: Perotti, Christian. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; ArgentinaFil: Ortel, Bernhard. Harvard Medical School; Estados UnidosFil: Di Venosa, Gabriela Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; ArgentinaFil: Saccoliti, María. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Carlos Durand; ArgentinaFil: Batlle, Alcira Maria del C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; ArgentinaFil: Hasan, Tayyaba. Harvard Medical School; Estados UnidosSpandidos Publications2006-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/152593Casas, Adriana Gabriela; Perotti, Christian; Ortel, Bernhard; Di Venosa, Gabriela Mariana; Saccoliti, María; et al.; Tumor cell lines resistant to ALA-mediated photodynamic therapy and possible tools to target surviving cells; Spandidos Publications; International Journal of Oncology; 29; 2; 8-2006; 397-4051019-6439CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.spandidos-publications.com/ijo/29/2/397info:eu-repo/semantics/altIdentifier/doi/10.3892/ijo.29.2.397info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:03Zoai:ri.conicet.gov.ar:11336/152593instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:03.491CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Tumor cell lines resistant to ALA-mediated photodynamic therapy and possible tools to target surviving cells |
title |
Tumor cell lines resistant to ALA-mediated photodynamic therapy and possible tools to target surviving cells |
spellingShingle |
Tumor cell lines resistant to ALA-mediated photodynamic therapy and possible tools to target surviving cells Casas, Adriana Gabriela AMINOLEVULINIC ACID CROSS-RESISTANCE HYPOXIA PHOTODYNAMIC THERAPY RESISTANCE |
title_short |
Tumor cell lines resistant to ALA-mediated photodynamic therapy and possible tools to target surviving cells |
title_full |
Tumor cell lines resistant to ALA-mediated photodynamic therapy and possible tools to target surviving cells |
title_fullStr |
Tumor cell lines resistant to ALA-mediated photodynamic therapy and possible tools to target surviving cells |
title_full_unstemmed |
Tumor cell lines resistant to ALA-mediated photodynamic therapy and possible tools to target surviving cells |
title_sort |
Tumor cell lines resistant to ALA-mediated photodynamic therapy and possible tools to target surviving cells |
dc.creator.none.fl_str_mv |
Casas, Adriana Gabriela Perotti, Christian Ortel, Bernhard Di Venosa, Gabriela Mariana Saccoliti, María Batlle, Alcira Maria del C. Hasan, Tayyaba |
author |
Casas, Adriana Gabriela |
author_facet |
Casas, Adriana Gabriela Perotti, Christian Ortel, Bernhard Di Venosa, Gabriela Mariana Saccoliti, María Batlle, Alcira Maria del C. Hasan, Tayyaba |
author_role |
author |
author2 |
Perotti, Christian Ortel, Bernhard Di Venosa, Gabriela Mariana Saccoliti, María Batlle, Alcira Maria del C. Hasan, Tayyaba |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
AMINOLEVULINIC ACID CROSS-RESISTANCE HYPOXIA PHOTODYNAMIC THERAPY RESISTANCE |
topic |
AMINOLEVULINIC ACID CROSS-RESISTANCE HYPOXIA PHOTODYNAMIC THERAPY RESISTANCE |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.2 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
We isolated and characterized cell lines resistant to aminolevulinic acid (ALA)-mediated photodynamic therapy (PDT) derived from a murine adenocarcinoma and studied cross resistance with other injuries. The most resistant clones were numbers 4 and 8, which exhibited 6.7- and 4.2-fold increase in resistance respectively. Several characteristics were altered in these clones. A 2-fold increase in cell volume, higher cell spreading, and a more fibroblastic, dendritic pattern, were the morphology features that led us to think they could have different adhesive, invasive or metastatic phenotypes. The amount of porphyrins synthesized per cell in the resistant clones was similar to the parental line but, when it was expressed per mg protein, there was a 2-fold decrease, with a higher proportion of hydrophilic porphyrins. These cells were not cross-resistant to photosensitization with Benzoporphyrin derivative and Merocyanine 540, but exhibited a slight resistance to exogenous protoporphyrin IX treatment. Both clones displayed higher protein content and increased number of mitochondria, together with a higher oxygen consumption. The distinctive features found in the resistant lines led as to think how to exploit the changes induced by PDT treatment to target surviving cells. Those hypoxic cells can be also a preferential target of bioreductive drugs and hypoxia-directed gene therapy, and would be sensitive to treatment with other photosensitizers. Fil: Casas, Adriana Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina Fil: Perotti, Christian. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina Fil: Ortel, Bernhard. Harvard Medical School; Estados Unidos Fil: Di Venosa, Gabriela Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina Fil: Saccoliti, María. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Carlos Durand; Argentina Fil: Batlle, Alcira Maria del C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Investigaciones sobre Porfirinas y Porfirias. Universidad de Buenos Aires. Centro de Investigaciones sobre Porfirinas y Porfirias; Argentina Fil: Hasan, Tayyaba. Harvard Medical School; Estados Unidos |
description |
We isolated and characterized cell lines resistant to aminolevulinic acid (ALA)-mediated photodynamic therapy (PDT) derived from a murine adenocarcinoma and studied cross resistance with other injuries. The most resistant clones were numbers 4 and 8, which exhibited 6.7- and 4.2-fold increase in resistance respectively. Several characteristics were altered in these clones. A 2-fold increase in cell volume, higher cell spreading, and a more fibroblastic, dendritic pattern, were the morphology features that led us to think they could have different adhesive, invasive or metastatic phenotypes. The amount of porphyrins synthesized per cell in the resistant clones was similar to the parental line but, when it was expressed per mg protein, there was a 2-fold decrease, with a higher proportion of hydrophilic porphyrins. These cells were not cross-resistant to photosensitization with Benzoporphyrin derivative and Merocyanine 540, but exhibited a slight resistance to exogenous protoporphyrin IX treatment. Both clones displayed higher protein content and increased number of mitochondria, together with a higher oxygen consumption. The distinctive features found in the resistant lines led as to think how to exploit the changes induced by PDT treatment to target surviving cells. Those hypoxic cells can be also a preferential target of bioreductive drugs and hypoxia-directed gene therapy, and would be sensitive to treatment with other photosensitizers. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/152593 Casas, Adriana Gabriela; Perotti, Christian; Ortel, Bernhard; Di Venosa, Gabriela Mariana; Saccoliti, María; et al.; Tumor cell lines resistant to ALA-mediated photodynamic therapy and possible tools to target surviving cells; Spandidos Publications; International Journal of Oncology; 29; 2; 8-2006; 397-405 1019-6439 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/152593 |
identifier_str_mv |
Casas, Adriana Gabriela; Perotti, Christian; Ortel, Bernhard; Di Venosa, Gabriela Mariana; Saccoliti, María; et al.; Tumor cell lines resistant to ALA-mediated photodynamic therapy and possible tools to target surviving cells; Spandidos Publications; International Journal of Oncology; 29; 2; 8-2006; 397-405 1019-6439 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.spandidos-publications.com/ijo/29/2/397 info:eu-repo/semantics/altIdentifier/doi/10.3892/ijo.29.2.397 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Spandidos Publications |
publisher.none.fl_str_mv |
Spandidos Publications |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1842268641824866304 |
score |
13.13397 |