A side-by-side comparison of different capacitation media in developing mouse sperm fertilizing ability

Autores
González, Lucas Nicolás; Giaccagli, Maria Milagros; Herzfeld, Jael Dafne; Cuasnicu, Patricia Sara; Da Ros, Vanina Gabriela; Cohen, Debora Juana
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To acquire the ability to fertilize the egg, mammalian spermatozoa must undergo a series of changes occurring within the highly synchronized and specialized environment of the female reproductive tract, collectively known as capacitation. In an attempt to replicate this process in vitro, various culture media for mouse sperm were formulated over the past decades, sharing a similar overall composition but difering mainly in ion concentrations and metabolic substrates. The widespread use of the diferent media to study the mechanisms of capacitation might hinder a comprehensive understanding of this process, as the medium could become a confounding variable in the analysis. In this context, the present side-by-side study compares the infuence of four commonly used culture media (FD, HTF and two TYH versions) on mouse sperm capacitation. We evaluated the induction of protein kinase A phosphorylation pathway, motility, hyperactivation and acrosome reaction. Additionally, in vitro fertilization and embryo development were also assessed. By analyzing these outcomes in two mouse colonies with diferent reproductive performance, our study provides critical insights to improve the global understanding of sperm function. The results obtained highlight the importance of considering variations in medium composition, and their potential implications for the future interpretation of results.
Fil: González, Lucas Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Giaccagli, Maria Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Herzfeld, Jael Dafne. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Da Ros, Vanina Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Materia
SPERM CAPACITATION
ACROSOME REACTION
FERTILIZATION
EMBRYONIC DEVELOPMENT
SPERMATOZOA
PHOSPORYLATION
HYPERACTIVATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242678
Acceder
id CONICETDig_afcc99b5d266a4d72f011727940b065c
oai_identifier_str oai:ri.conicet.gov.ar:11336/242678
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A side-by-side comparison of different capacitation media in developing mouse sperm fertilizing abilityGonzález, Lucas NicolásGiaccagli, Maria MilagrosHerzfeld, Jael DafneCuasnicu, Patricia SaraDa Ros, Vanina GabrielaCohen, Debora JuanaSPERM CAPACITATIONACROSOME REACTIONFERTILIZATIONEMBRYONIC DEVELOPMENTSPERMATOZOAPHOSPORYLATIONHYPERACTIVATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1To acquire the ability to fertilize the egg, mammalian spermatozoa must undergo a series of changes occurring within the highly synchronized and specialized environment of the female reproductive tract, collectively known as capacitation. In an attempt to replicate this process in vitro, various culture media for mouse sperm were formulated over the past decades, sharing a similar overall composition but difering mainly in ion concentrations and metabolic substrates. The widespread use of the diferent media to study the mechanisms of capacitation might hinder a comprehensive understanding of this process, as the medium could become a confounding variable in the analysis. In this context, the present side-by-side study compares the infuence of four commonly used culture media (FD, HTF and two TYH versions) on mouse sperm capacitation. We evaluated the induction of protein kinase A phosphorylation pathway, motility, hyperactivation and acrosome reaction. Additionally, in vitro fertilization and embryo development were also assessed. By analyzing these outcomes in two mouse colonies with diferent reproductive performance, our study provides critical insights to improve the global understanding of sperm function. The results obtained highlight the importance of considering variations in medium composition, and their potential implications for the future interpretation of results.Fil: González, Lucas Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Giaccagli, Maria Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Herzfeld, Jael Dafne. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Da Ros, Vanina Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaSpringer2024-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242678González, Lucas Nicolás; Giaccagli, Maria Milagros; Herzfeld, Jael Dafne; Cuasnicu, Patricia Sara; Da Ros, Vanina Gabriela; et al.; A side-by-side comparison of different capacitation media in developing mouse sperm fertilizing ability; Springer; Scientific Reports; 14; 1; 6-2024; 1-132045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-024-65134-winfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-024-65134-winfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:33Zoai:ri.conicet.gov.ar:11336/242678instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:33.527CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A side-by-side comparison of different capacitation media in developing mouse sperm fertilizing ability
title A side-by-side comparison of different capacitation media in developing mouse sperm fertilizing ability
spellingShingle A side-by-side comparison of different capacitation media in developing mouse sperm fertilizing ability
González, Lucas Nicolás
SPERM CAPACITATION
ACROSOME REACTION
FERTILIZATION
EMBRYONIC DEVELOPMENT
SPERMATOZOA
PHOSPORYLATION
HYPERACTIVATION
title_short A side-by-side comparison of different capacitation media in developing mouse sperm fertilizing ability
title_full A side-by-side comparison of different capacitation media in developing mouse sperm fertilizing ability
title_fullStr A side-by-side comparison of different capacitation media in developing mouse sperm fertilizing ability
title_full_unstemmed A side-by-side comparison of different capacitation media in developing mouse sperm fertilizing ability
title_sort A side-by-side comparison of different capacitation media in developing mouse sperm fertilizing ability
dc.creator.none.fl_str_mv González, Lucas Nicolás
Giaccagli, Maria Milagros
Herzfeld, Jael Dafne
Cuasnicu, Patricia Sara
Da Ros, Vanina Gabriela
Cohen, Debora Juana
author González, Lucas Nicolás
author_facet González, Lucas Nicolás
Giaccagli, Maria Milagros
Herzfeld, Jael Dafne
Cuasnicu, Patricia Sara
Da Ros, Vanina Gabriela
Cohen, Debora Juana
author_role author
author2 Giaccagli, Maria Milagros
Herzfeld, Jael Dafne
Cuasnicu, Patricia Sara
Da Ros, Vanina Gabriela
Cohen, Debora Juana
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv SPERM CAPACITATION
ACROSOME REACTION
FERTILIZATION
EMBRYONIC DEVELOPMENT
SPERMATOZOA
PHOSPORYLATION
HYPERACTIVATION
topic SPERM CAPACITATION
ACROSOME REACTION
FERTILIZATION
EMBRYONIC DEVELOPMENT
SPERMATOZOA
PHOSPORYLATION
HYPERACTIVATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv To acquire the ability to fertilize the egg, mammalian spermatozoa must undergo a series of changes occurring within the highly synchronized and specialized environment of the female reproductive tract, collectively known as capacitation. In an attempt to replicate this process in vitro, various culture media for mouse sperm were formulated over the past decades, sharing a similar overall composition but difering mainly in ion concentrations and metabolic substrates. The widespread use of the diferent media to study the mechanisms of capacitation might hinder a comprehensive understanding of this process, as the medium could become a confounding variable in the analysis. In this context, the present side-by-side study compares the infuence of four commonly used culture media (FD, HTF and two TYH versions) on mouse sperm capacitation. We evaluated the induction of protein kinase A phosphorylation pathway, motility, hyperactivation and acrosome reaction. Additionally, in vitro fertilization and embryo development were also assessed. By analyzing these outcomes in two mouse colonies with diferent reproductive performance, our study provides critical insights to improve the global understanding of sperm function. The results obtained highlight the importance of considering variations in medium composition, and their potential implications for the future interpretation of results.
Fil: González, Lucas Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Giaccagli, Maria Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Herzfeld, Jael Dafne. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Da Ros, Vanina Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description To acquire the ability to fertilize the egg, mammalian spermatozoa must undergo a series of changes occurring within the highly synchronized and specialized environment of the female reproductive tract, collectively known as capacitation. In an attempt to replicate this process in vitro, various culture media for mouse sperm were formulated over the past decades, sharing a similar overall composition but difering mainly in ion concentrations and metabolic substrates. The widespread use of the diferent media to study the mechanisms of capacitation might hinder a comprehensive understanding of this process, as the medium could become a confounding variable in the analysis. In this context, the present side-by-side study compares the infuence of four commonly used culture media (FD, HTF and two TYH versions) on mouse sperm capacitation. We evaluated the induction of protein kinase A phosphorylation pathway, motility, hyperactivation and acrosome reaction. Additionally, in vitro fertilization and embryo development were also assessed. By analyzing these outcomes in two mouse colonies with diferent reproductive performance, our study provides critical insights to improve the global understanding of sperm function. The results obtained highlight the importance of considering variations in medium composition, and their potential implications for the future interpretation of results.
publishDate 2024
dc.date.none.fl_str_mv 2024-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242678
González, Lucas Nicolás; Giaccagli, Maria Milagros; Herzfeld, Jael Dafne; Cuasnicu, Patricia Sara; Da Ros, Vanina Gabriela; et al.; A side-by-side comparison of different capacitation media in developing mouse sperm fertilizing ability; Springer; Scientific Reports; 14; 1; 6-2024; 1-13
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242678
identifier_str_mv González, Lucas Nicolás; Giaccagli, Maria Milagros; Herzfeld, Jael Dafne; Cuasnicu, Patricia Sara; Da Ros, Vanina Gabriela; et al.; A side-by-side comparison of different capacitation media in developing mouse sperm fertilizing ability; Springer; Scientific Reports; 14; 1; 6-2024; 1-13
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-024-65134-w
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-024-65134-w
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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