Tslc1 (nectin-like molecule-2) is essential for spermatozoa motility and male fertility

Autores
Surace, Ezequiel Ignacio; Strickland, Amy; Hess, Rex A.; Gutmann, David H.; Naughton, Cathy K.
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The nectin-like molecule-2 (TSLC1) is a cell-cell adhesion molecule expressed in testicular germ cells. To directly examine the role of Tslc1 in male fertility, we generated Tslc1+/-mice that have greater than 90% reduction in Tslc1 expression. Tslc1+/- males exhibited reduced fertility and rarely transmitted the Tslc1 mutant allele, whereas Tslc1+/- females were consistently able to transmit the mutant allele. Histologic and electron microscopic analyses of the testes in Tslc1+/- mice demonstrated disruption of the junctional scaffold between germ cells and Sertoli cells. Reduced Tslc1 expression had no effect on germ cell proliferation or apoptosis. While evidence of normal spermatozoal maturation was supported by Fluorescence Activated Cell Sorting (FACS) analysis, spermatozoa from Tslc1+/- mice demonstrated markedly reduced motility without compromised viability. Collectively, these results establish an essential role for Tslc1 in spermatozoal maturation and motility, distinct from other members of the nectin family.
Fil: Surace, Ezequiel Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Washington University in St. Louis; Estados Unidos
Fil: Strickland, Amy. Washington University in St. Louis; Estados Unidos
Fil: Hess, Rex A.. University of Illinois; Estados Unidos
Fil: Gutmann, David H.. Washington University in St. Louis; Estados Unidos
Fil: Naughton, Cathy K.. Washington University in St. Louis; Estados Unidos
Materia
IGSF4
SPERMATOGENESIS
SPERMIOGENESIS
SYN-CAM1
TESTIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39068
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Tslc1 (nectin-like molecule-2) is essential for spermatozoa motility and male fertilitySurace, Ezequiel IgnacioStrickland, AmyHess, Rex A.Gutmann, David H.Naughton, Cathy K.IGSF4SPERMATOGENESISSPERMIOGENESISSYN-CAM1TESTIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The nectin-like molecule-2 (TSLC1) is a cell-cell adhesion molecule expressed in testicular germ cells. To directly examine the role of Tslc1 in male fertility, we generated Tslc1+/-mice that have greater than 90% reduction in Tslc1 expression. Tslc1+/- males exhibited reduced fertility and rarely transmitted the Tslc1 mutant allele, whereas Tslc1+/- females were consistently able to transmit the mutant allele. Histologic and electron microscopic analyses of the testes in Tslc1+/- mice demonstrated disruption of the junctional scaffold between germ cells and Sertoli cells. Reduced Tslc1 expression had no effect on germ cell proliferation or apoptosis. While evidence of normal spermatozoal maturation was supported by Fluorescence Activated Cell Sorting (FACS) analysis, spermatozoa from Tslc1+/- mice demonstrated markedly reduced motility without compromised viability. Collectively, these results establish an essential role for Tslc1 in spermatozoal maturation and motility, distinct from other members of the nectin family.Fil: Surace, Ezequiel Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Washington University in St. Louis; Estados UnidosFil: Strickland, Amy. Washington University in St. Louis; Estados UnidosFil: Hess, Rex A.. University of Illinois; Estados UnidosFil: Gutmann, David H.. Washington University in St. Louis; Estados UnidosFil: Naughton, Cathy K.. Washington University in St. Louis; Estados UnidosAmerican Society of Andrology2006-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39068Surace, Ezequiel Ignacio; Strickland, Amy; Hess, Rex A.; Gutmann, David H.; Naughton, Cathy K.; Tslc1 (nectin-like molecule-2) is essential for spermatozoa motility and male fertility; American Society of Andrology; Journal of Andrology; 27; 6; 11-2006; 816-8250196-3635939-4640CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.2164/jandrol.106.000398/fullinfo:eu-repo/semantics/altIdentifier/doi/10.2164/jandrol.106.000398info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2755522/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:13Zoai:ri.conicet.gov.ar:11336/39068instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:13.844CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Tslc1 (nectin-like molecule-2) is essential for spermatozoa motility and male fertility
title Tslc1 (nectin-like molecule-2) is essential for spermatozoa motility and male fertility
spellingShingle Tslc1 (nectin-like molecule-2) is essential for spermatozoa motility and male fertility
Surace, Ezequiel Ignacio
IGSF4
SPERMATOGENESIS
SPERMIOGENESIS
SYN-CAM1
TESTIS
title_short Tslc1 (nectin-like molecule-2) is essential for spermatozoa motility and male fertility
title_full Tslc1 (nectin-like molecule-2) is essential for spermatozoa motility and male fertility
title_fullStr Tslc1 (nectin-like molecule-2) is essential for spermatozoa motility and male fertility
title_full_unstemmed Tslc1 (nectin-like molecule-2) is essential for spermatozoa motility and male fertility
title_sort Tslc1 (nectin-like molecule-2) is essential for spermatozoa motility and male fertility
dc.creator.none.fl_str_mv Surace, Ezequiel Ignacio
Strickland, Amy
Hess, Rex A.
Gutmann, David H.
Naughton, Cathy K.
author Surace, Ezequiel Ignacio
author_facet Surace, Ezequiel Ignacio
Strickland, Amy
Hess, Rex A.
Gutmann, David H.
Naughton, Cathy K.
author_role author
author2 Strickland, Amy
Hess, Rex A.
Gutmann, David H.
Naughton, Cathy K.
author2_role author
author
author
author
dc.subject.none.fl_str_mv IGSF4
SPERMATOGENESIS
SPERMIOGENESIS
SYN-CAM1
TESTIS
topic IGSF4
SPERMATOGENESIS
SPERMIOGENESIS
SYN-CAM1
TESTIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The nectin-like molecule-2 (TSLC1) is a cell-cell adhesion molecule expressed in testicular germ cells. To directly examine the role of Tslc1 in male fertility, we generated Tslc1+/-mice that have greater than 90% reduction in Tslc1 expression. Tslc1+/- males exhibited reduced fertility and rarely transmitted the Tslc1 mutant allele, whereas Tslc1+/- females were consistently able to transmit the mutant allele. Histologic and electron microscopic analyses of the testes in Tslc1+/- mice demonstrated disruption of the junctional scaffold between germ cells and Sertoli cells. Reduced Tslc1 expression had no effect on germ cell proliferation or apoptosis. While evidence of normal spermatozoal maturation was supported by Fluorescence Activated Cell Sorting (FACS) analysis, spermatozoa from Tslc1+/- mice demonstrated markedly reduced motility without compromised viability. Collectively, these results establish an essential role for Tslc1 in spermatozoal maturation and motility, distinct from other members of the nectin family.
Fil: Surace, Ezequiel Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Washington University in St. Louis; Estados Unidos
Fil: Strickland, Amy. Washington University in St. Louis; Estados Unidos
Fil: Hess, Rex A.. University of Illinois; Estados Unidos
Fil: Gutmann, David H.. Washington University in St. Louis; Estados Unidos
Fil: Naughton, Cathy K.. Washington University in St. Louis; Estados Unidos
description The nectin-like molecule-2 (TSLC1) is a cell-cell adhesion molecule expressed in testicular germ cells. To directly examine the role of Tslc1 in male fertility, we generated Tslc1+/-mice that have greater than 90% reduction in Tslc1 expression. Tslc1+/- males exhibited reduced fertility and rarely transmitted the Tslc1 mutant allele, whereas Tslc1+/- females were consistently able to transmit the mutant allele. Histologic and electron microscopic analyses of the testes in Tslc1+/- mice demonstrated disruption of the junctional scaffold between germ cells and Sertoli cells. Reduced Tslc1 expression had no effect on germ cell proliferation or apoptosis. While evidence of normal spermatozoal maturation was supported by Fluorescence Activated Cell Sorting (FACS) analysis, spermatozoa from Tslc1+/- mice demonstrated markedly reduced motility without compromised viability. Collectively, these results establish an essential role for Tslc1 in spermatozoal maturation and motility, distinct from other members of the nectin family.
publishDate 2006
dc.date.none.fl_str_mv 2006-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39068
Surace, Ezequiel Ignacio; Strickland, Amy; Hess, Rex A.; Gutmann, David H.; Naughton, Cathy K.; Tslc1 (nectin-like molecule-2) is essential for spermatozoa motility and male fertility; American Society of Andrology; Journal of Andrology; 27; 6; 11-2006; 816-825
0196-3635
939-4640
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39068
identifier_str_mv Surace, Ezequiel Ignacio; Strickland, Amy; Hess, Rex A.; Gutmann, David H.; Naughton, Cathy K.; Tslc1 (nectin-like molecule-2) is essential for spermatozoa motility and male fertility; American Society of Andrology; Journal of Andrology; 27; 6; 11-2006; 816-825
0196-3635
939-4640
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.2164/jandrol.106.000398/full
info:eu-repo/semantics/altIdentifier/doi/10.2164/jandrol.106.000398
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2755522/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Andrology
publisher.none.fl_str_mv American Society of Andrology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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